ライフサイエンスコーパス: cutinase

1 was more pronounced for the lipase than the cutinase.

2 ed this activity with putative mycobacterial cutinase.

3 bound efficiently and specifically to LFA-1/cutinase.

4 ceptor that presents the nonmammalian enzyme cutinase.

5 y Rhizopus oryzae lipase and Fusarium solani cutinase.

6 imilar tertiary structure is Fusarium solani cutinase.

7 ut2/3 matched with that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F

8 assemblies can much more effectively retain cutinase 2 activity on a surface after co-incubation and

9 hese hydrophobins and their interaction with cutinase 2 is crucial for the development of novel antif

10 ith that of the peptides from cutinase 1 and cutinase 2, respectively, isolated from F. solani pisi g

11 esion and to direct the action of the enzyme cutinase 2, resulting in penetration of the plant host.

12 ability, and structure of Aspergillus oryzae cutinase and compare it to the well-studied enzyme from

13 Protein fusions between cutinase and five antibodies of three different types (s

14 Here we used the fungal enzyme cutinase and its suicide substrate p-nitrophenyl phospho

15 sity, using the covalent interaction between cutinase and its suicide substrate.

16 Conversely, Fusarium solani Cutinase and lipases from Mycobacterium tuberculosis (Rv

17 t encounter cutin; we demonstrate that known cutinases and MPLA cleave phospholipids in a PLA-type ma

18 The covalent immobilization is specific for cutinase, and the glycol-terminated monolayer effectivel

19 These structural features of A. oryzae cutinase are proposed to result in an improved hydrolyti

20 Cutinases are responsible for hydrolysis of the protecti

21 Cutinases are serine esterases whose primary substrate i

22 mon resonance (SPR) spectroscopy showed that cutinase binds irreversibly to a monolayer presenting th

23 rganization with a catalytic triad common to cutinases, but which contains an additional four-helix d

24 ound to be essential for the inducibility of cutinase by hydroxy fatty acids.

25 dies suggest an alternative use for putative cutinases by the M. tuberculosis group that is likely re

26 e used to immobilize proteins of interest, a cutinase-calmodulin fusion protein was constructed and i

27 ionalized with a novel calmodulin construct, cutinase-calmodulin-cutinase (CutCaMCut), reversibly shi

28 Yet, the cutinase completely hydrolyzed all PBAT films, including

29 el calmodulin construct, cutinase-calmodulin-cutinase (CutCaMCut), reversibly shifts by 2-3 nm.

30 The demonstration of cutinase-directed antibody immobilization with insert SA

31 Based on the in-silico search, a cutinase from Pseudomonas pseudoalcaligenes (PpCutA) and

32 (butylene adipate) films and Fusarium solani cutinase (FsC).

33 c sequence located at -159 base pairs of the cutinase gene in Fusarium solani f. sp. pisi (Nectria he

34 y binding selectively to palindrome 2 of the cutinase gene promoter.

35 n previously to induce the expression of the cutinase gene via a palindromic sequence located at -159

36 We cloned three highly homologous cutinase genes, cut1, cut2, and cut3, from Fusarium sola

37 Asp175-His188-Ser120 of the serine esterase cutinase in the ground state.

38 y the low levels of constitutively expressed cutinase, induce high levels of cutinase that can help p

39 ed here provide insight into engineering new cutinase-inspired biocatalysts with tailor-made properti

40 uronidase gene fused to the promoters of the cutinases integrated into F. solani pisi genome indicate

41 We analyzed cutinase motifs in mycobacteria and found the motif very

42 Action of this cell-surface cutinase on enzyme substrate self-assembled monolayers s

43 SV40 in yeast, it transactivated the native cutinase promoter fused to the chloramphenicol acetyl tr

44 of three microbial lipases: Fusarium solani cutinase, Rv0183, and LipY from Mycobacterium tuberculos

45 ly expressed cutinase, induce high levels of cutinase that can help pathogenic fungi to penetrate int

46 m tuberculosis has maintained seven putative cutinases, though it should not encounter cutin; we demo

47 y is based on binding of the serine esterase cutinase to a self-assembled monolayer presenting a phos

48 cDNA clone encoding a polypeptide designated cutinase transcription factor 1alpha (CTF1alpha) with a

49 flavodoxin family (CheY, apoflavodoxin, and cutinase) using a simple nucleation and growth model tha

50 Cutinase was embedded in the extracellular domain of LFA

51 Cutinase was highly diastereoselective for the (Sp) conf

52 Further changes in the dynamics of cutinase were determined from quasiharmonic mode analysi

53 Nonlocal effects on the structure of cutinase were observed.