ライフサイエンスコーパス: cyan

1 nced GFP (EGFP) spectral variants yellow and cyan.

2 ed, suggesting that these cells might be red-cyan.

3 bunit was tagged with yellow (alpha1-YFP) or cyan (alpha1-CFP) fluorescent protein.

4 coral Montipora capitata consistently emits cyan and red fluorescence across a depth gradient in ree

5 e been responsible for the generation of new cyan and red phenotypes from the ancestral green were fi

6 nce resonance energy transfer (FRET) between cyan and yellow fluorescent (CFP/YFP) fusion proteins of

7 HSP-FRET consists of the cyan and yellow fluorescent protein fluorophores linked

8 Using cyan and yellow fluorescent protein fusion constructs, w

9 of mutant and wild-type (WT) M2 regions, of cyan and yellow fluorescent protein, and of fluorescent

10 at either the N or C termini, and a pair of cyan and yellow fluorescent protein-tagged tau were co-t

11 one another, we have constructed a tandem of cyan and yellow fluorescent proteins (CFP and YFP, respe

12 ce energy transfer (FRET) between the linked cyan and yellow fluorescent proteins (CFP and YFP, respe

13 ance energy transfer (FRET) between enhanced cyan and yellow fluorescent proteins (ECFP, EYFP) in liv

14 While pairs of cyan and yellow fluorescent proteins (FPs) are most comm

15 ains, each of which coexpresses the enhanced cyan and yellow fluorescent proteins as fusions to disti

16 luorescent resonance energy transfer between cyan and yellow fluorescent proteins conjugated at its N

17 e sensor, termed CAY, is a fusion protein of cyan and yellow fluorescent proteins flanking the peptid

18 ive variant of recombinant WNV NS2B-NS3, and cyan and yellow fluorescent proteins fused by a dodecame

19 The cyan and yellow fluorescent proteins were attached to fu

20 orescent proteins (IFPs), such as the green, cyan and yellow fluorescent proteins, have revolutionize

21 r (FRET) glutamine sensors based on improved cyan and yellow fluorescent proteins, monomeric Teal Flu

22 nce resonance energy transfer (FRET) between cyan and yellow fluorescent proteins.

23 ) between alpha- and beta- subunits fused to cyan and yellow fluorescent proteins.

24 ntaining acidic amino acid residues, between cyan and yellow mutants of GFP.

25 by sandwiching the full-length Epac1 between cyan and yellow mutants of GFP.

26 kinase (cGPK), minus residues 1-77, between cyan and yellow mutants of green fluorescent protein.

27 e resonance energy transfer (FRET) between a cyan and yellow variant of GFP fused to the termini of t

28 Next, we create cyan and yellow variants, achieving activity-dependent,

29 sfer between those two proteins labeled with cyan and yellow-green variants of green fluorescent prot

30 ities of coexpressed CFP-C-beta1CFP-N-gamma (cyan) and CFP-C-beta1YFP-N-gamma2 (yellow) complexes wer

31 orescent proteins to generate green, yellow, cyan, and red reporters, paving the way for multiplex pr

32 Cyan- and blue-elicited fluorescence have different sens

33 let], [green/yellow], [blue], [orange], and [cyan], appearing in this order, is recovered, featuring

34 In summary, red-green (or red-cyan) cells, along with blue-yellow and black-white cell

35 orter, NKCC1, we tagged the transporter with cyan (CFP) and yellow (YFP) fluorescent proteins and mea

36 ion of NKCC1, we tagged the transporter with cyan (CFP) and yellow (YFP) fluorescent proteins at two

37 Cyan (CFP) and yellow (YFP) fluorescent proteins were us

38 iving cells, these proteins were tagged with cyan (CFP) and yellow (YFP) mutants of the green fluores

39 Yellow (YFP) and cyan (CFP) fluorescent proteins were linked to the NH(2)

40 This protein is a member of the cyan class of Anthozoa FPs and exhibits broad, double-hu

41 rise to green fluorescence as opposed to the cyan color of emission that is characteristic for the ne

42 transfer (FRET) acceptor protein, emitting a cyan-colored fluorescence with an unusually long excited

43 s expressing 5-HT(2C) receptors labeled with cyan (donor) and yellow (acceptor) fluorescent proteins.

44 of spectrally distinct GFPs such as blue or cyan donors in combination with green or yellow acceptor

45 e transcription and the indirect coupling of Cyan dyes.

46 s with a 15 to 30% increase in the yellow-to-cyan emission ratio because of a phosphorylation-depende

47 e fluorescent proteins, increasing yellow to cyan emission ratios by 10-30%.

48 o gain insight into the structural basis for cyan emission, the crystal structure of amFP486 (lambda(

49 es 20-35% changes in the ratios of yellow to cyan emissions because of phosphorylation-induced change

50 se 25-50% changes in the ratios of yellow to cyan emissions in live cells caused by phosphorylation-i

51 G-protein subunit beta 1 (YFP-beta 1) and a cyan-emitting GFP mutant fused to the N-terminus of the

52 eon' (Ycam2) - comprising a fusion between a cyan-emitting mutant of the green fluorescent protein (G

53 They consist of tandem fusions of a blue- or cyan-emitting mutant of the green fluorescent protein (G

54 izes a newly developed FRET donor, monomeric cyan-excitable red fluorescent protein (mCyRFP1), which

55 in locus control region driving the enhanced cyan fluorescence protein (ECFP) gene.

56 three positive control constructs in which a cyan fluorescence protein and a yellow fluorescence prot

57 cence protein-tagged RGS7.Gbeta5 complex and cyan fluorescence protein-tagged Galphaq, indicating a d

58 inetics of one representative of this class (cyan fluorescence protein/yellow fluorescent protein-flu

59 energy transfer suggests that GAT1-YFP8 and cyan fluorescent (CFP) tagged ezrin (ezrin-CFP) exist wi

60 These mice exhibit cyan fluorescent cells specifically in the erythroid com

61 Finally, C-terminal yellow and cyan fluorescent fusion proteins, AT1R-YFP and BK-CFP, d

62 ts of yellow fluorescent protein (Venus) and cyan fluorescent protein (Cerulean) flank either the ent

63 reticulum Ca2+-ATPase (SERCA) were fused to cyan fluorescent protein (CFP) and coexpressed with PLB

64 maging to detect the proximity between CXCR1-cyan fluorescent protein (CFP) and fluorescence probes t

65 n a pixel-by-pixel basis using EGFR fused to cyan fluorescent protein (CFP) and Grb2 fused to yellow

66 To study this interaction, cyan fluorescent protein (CFP) and yellow fluorescent pr

67 FP-APP-YFP [containing the fluorescent tags, cyan fluorescent protein (CFP) and yellow fluorescent pr

68 The MetN was sandwiched between cyan fluorescent protein (CFP) and yellow fluorescent pr

69 r resonance energy transfer (FRET) pair, the cyan fluorescent protein (CFP) and yellow fluorescent pr

70 sfer (FRET) pairs with distinct spectra: (a) cyan fluorescent protein (CFP) and yellow FP (YFP), and

71 nsfer (FRET) of beta1a subunits labeled with cyan fluorescent protein (CFP) and/or yellow fluorescent

72 The methodology uses the cyan fluorescent protein (CFP) as a biomass indicator an

73 When tPA was imaged simultaneously with cyan fluorescent protein (CFP) as a cytosolic marker, th

74 odel cell system and the standard FRET pair, cyan fluorescent protein (CFP) as the donor and yellow f

75 length Kir6.2 subunits were linked to YFP or cyan fluorescent protein (CFP) at N or C termini, and al

76 nalysis of the subcellular localization of a cyan fluorescent protein (CFP) fusion and a protein-prot

77 ellow fluorescent protein (YFP) and enhanced cyan fluorescent protein (CFP) genes in which recombinat

78 Co-expression of Wld protein and cyan fluorescent protein (CFP) in axons and neuromuscula

79 alphas-CFP, with cyan fluorescent protein (CFP) inserted into an internal

80 epithelial (LLCPK) cells expressing stathmin-cyan fluorescent protein (CFP) or injected with stathmin

81 ignalling (RGS4) proteins were each fused to cyan fluorescent protein (CFP) or yellow fluorescent pro

82 by two nonfluorescent fragments (N and C) of cyan fluorescent protein (CFP) or yellow fluorescent pro

83 of Grb2, Shc, H-Ras, and K-Ras with enhanced cyan fluorescent protein (CFP) or yellow fluorescent pro

84 -length and truncated forms of VacA fused to cyan fluorescent protein (CFP) or yellow fluorescent pro

85 Mice that expressed cyan fluorescent protein (CFP) or yellow fluorescent pro

86 with various vector combinations to express cyan fluorescent protein (CFP) or YFP fused to either bi

87 Fusions of cyan fluorescent protein (CFP) to MldA, MldB, and MldC r

88 otein of 25 kDa (SNAP-25), were used to link cyan fluorescent protein (CFP) to yellow fluorescent pro

89 nal ribosome entry sequence (IRES)-dependent cyan fluorescent protein (CFP) translation were monitore

90 Mice expressing cyan fluorescent protein (CFP) under control of the Thy-

91 strain was used, containing a construct with cyan fluorescent protein (CFP) under Thy-1 promoter cont

92 otein (YFP) was fused to the N terminus, and cyan fluorescent protein (CFP) was fused to the C termin

93 xtended with a transmembrane (TM) domain and cyan fluorescent protein (CFP) were immobilized in the p

94 The reporters consist of fusions of cyan fluorescent protein (CFP), a phosphotyrosine bindin

95 variants of green fluorescent protein (GFP), cyan fluorescent protein (CFP), and yellow fluorescent p

96 ROSA(tdTom), tryptophan hydroxylase 1 (Tph1)-cyan fluorescent protein (CFP), c-Kit(wsh/wsh), and Neur

97 ensor is composed of an end-to-end fusion of cyan fluorescent protein (CFP), chicken metallothionein

98 lly confirmed by measurements on mixtures of cyan fluorescent protein (CFP), citrine ((Cit) a yellow

99 icroscopic measurements of fluorescence from cyan fluorescent protein (CFP), citrine, and linked CFP-

100 Upon excitation of the cyan fluorescent protein (CFP), emission intensities of

101 ter resonance energy transfer (FRET) between cyan fluorescent protein (CFP)- and yellow fluorescent p

102 nance energy transmission (FRET) analysis of cyan fluorescent protein (CFP)-arm-CTD-yellow fluorescen

103 raction between MacMARCKS and dynamitin with cyan fluorescent protein (CFP)-conjugated dynamitin as t

104 RBPMS immunoreactivity is localized to cyan fluorescent protein (CFP)-fluorescent RGCs in the B

105 3)-AChR-yellow fluorescent protein (YFP) and cyan fluorescent protein (CFP)-Ggamma(2).

106 Transfection of C8PA cells with cyan fluorescent protein (CFP)-HSL, yellow fluorescent p

107 Finally, the level and rate of recovery of cyan fluorescent protein (CFP)-M1-5 were lower than thos

108 A functional cyan fluorescent protein (CFP)-tagged lac repressor-ER c

109 Cyan fluorescent protein (CFP)-tagged McpA, a stationary

110 performed following transient expression of cyan fluorescent protein (CFP)-tagged proteins and incub

111 ells, human DAT was fused to yellow (YFP) or cyan fluorescent protein (CFP).

112 -yellow fluorescent protein (YFP) and calnuc-cyan fluorescent protein (CFP).

113 tabilized by insertion into a loop in a host cyan fluorescent protein (CFP).

114 (FRET) between fusion proteins labeled with cyan fluorescent protein (donor) and yellow fluorescent

115 c mice expressing a synaptotagmin 1-enhanced cyan fluorescent protein (ECFP) fusion protein under con

116 llow fluorescent protein (EYFP) and enhanced cyan fluorescent protein (ECFP) variants of green fluore

117 id carrying the gene coding for the enhanced cyan fluorescent protein (ECFP) was also introduced into

118 protein (EYFP), Ht31 was linked to enhanced cyan fluorescent protein (ECFP), and these constructs we

119 rescent protein (GFP), a variant of enhanced cyan fluorescent protein (ECFP), has been determined to

120 yellow fluorescent protein (EYFP) > enhanced cyan fluorescent protein (ECFP), while a GST construct t

121 anced green fluorescent protein- or enhanced cyan fluorescent protein (ECFP)-tagged phospholipase Cde

122 ession of keratocan promoter-driven enhanced cyan fluorescent protein (ECFP).

123 Blue Fluorescent Protein (EBFP) and Enhanced Cyan Fluorescent Protein (ECFP).

124 Two PM markers, the enhanced cyan fluorescent protein (ECFP-Mem) and 3'-dioctadecylox

125 that contained enhanced yellow and enhanced cyan fluorescent protein (EYFP and ECFP, respectively) l

126 ion of tetanus toxin light chain tagged with cyan fluorescent protein (TNTCFP).

127 A recently engineered mutant of cyan fluorescent protein (WasCFP) that exhibits pH-depen

128 protein-Dictyostelium myosin II motor domain-cyan fluorescent protein (YFP-myosin-CFP) and compared t

129 otransfected with a mitochondrially targeted cyan fluorescent protein and an enhanced yellow fluoresc

130 onstrated by podocyte specific expression of cyan fluorescent protein and by electron microscopy.

131 red the interaction between DDR1 tagged with cyan fluorescent protein and DDR1 tagged with yellow flu

132 Fluorescent proteins enhanced cyan fluorescent protein and enhanced yellow fluorescent

133 ged the TAP1 and TAP2 subunits with enhanced cyan fluorescent protein and enhanced yellow fluorescent

134 Enhanced cyan fluorescent protein and enhanced yellow fluorescent

135 our method, we targeted QDs to cell surface cyan fluorescent protein and epidermal growth factor rec

136 human embryonic kidney 293T cells using H1R-cyan fluorescent protein and H2R-yellow fluorescent prot

137 Dual expression of SNAP-25 tagged with cyan fluorescent protein and VAMP-2 tagged with yellow f

138 omain of InsP3 receptors (types 1-3) between cyan fluorescent protein and yellow fluorescent protein

139 ing VP22 and VP13/14 as fusion proteins with cyan fluorescent protein and yellow fluorescent protein,

140 ding the human CrkII1-236 sandwiched between cyan fluorescent protein and yellow fluorescent protein,

141 tes inserted into a linker region separating cyan fluorescent protein and yellow fluorescent protein.

142 ubunits were genetically fused with enhanced cyan fluorescent protein and/or enhanced yellow fluoresc

143 s luciferase, yellow fluorescent protein, or cyan fluorescent protein at the carboxyl terminus of VPA

144 uced intracellular redistribution of an EGFR-cyan fluorescent protein chimera was markedly reduced by

145 Using ACE-fused cyan fluorescent protein donor and B2-fused yellow fluor

146 c for XOPS-mCFP, a membrane-targeted form of cyan fluorescent protein driven by the Xenopus rhodopsin

147 assays in plants that constitutively express cyan fluorescent protein fused to histone 2B provides en

148 an be rescued by overexpression of the PEX12-cyan fluorescent protein fusion protein, which targets t

149 oxisomes, as demonstrated for endogenous and cyan fluorescent protein fusion proteins by fluorescence

150 idate processing enzymes, which localized as cyan fluorescent protein fusions to mucocysts.

151 The advantage over previous constructs using cyan fluorescent protein is that our construct can be us

152 nally, chimeric proteins containing enhanced cyan fluorescent protein linked to wild-type CREB or CRE

153 fluorescent protein (YFP) and CD44 fused to cyan fluorescent protein on K562 cells.

154 s were addressed by tagging tapasin with the cyan fluorescent protein or yellow fluorescent protein (

155 ent colocalization of Akt2 fused with either cyan fluorescent protein or yellow fluorescent protein t

156 E12, E47, E12(NLS), or MyoD(NLS) and either cyan fluorescent protein or yellow fluorescent protein,

157 gion was used to introduce green, yellow and cyan fluorescent protein reporters into B. burgdorferi.

158 Using RD114-MFGS encoding cyan fluorescent protein to allow similar studies of nor

159 een genetically attached enhanced yellow and cyan fluorescent protein to the N or C terminus of the c

160 gitudinal retinal imaging of mice expressing cyan fluorescent protein under control of the Thy-1 prom

161 The retina of mice expressing cyan fluorescent protein under control of the Thy-1 prom

162 t the Trp66 position in the chromophore of a cyan fluorescent protein variant (CFP6) to investigate t

163 used to either yellow fluorescent protein or cyan fluorescent protein we can observe tau fusion prote

164 cell lines stably coexpressing PML-enhanced cyan fluorescent protein with other individual marker pr

165 f Escherichia coli K12 was flanked with CFP (cyan fluorescent protein) and YFP (yellow fluorescent pr

166 and double label (yellow fluorescent protein/cyan fluorescent protein) fluorescence labeling experime

167 e dynamic range and a 10% increase in donor (cyan fluorescent protein) fluorescence upon bleach of ye

168 almodulin, and the FRET donor ECFP (enhanced cyan fluorescent protein) into eNOS at a site adjacent t

169 a fluorescently tagged P-glycoprotein (MDR1-cyan fluorescent protein) permitted the drug-resistant p

170 in cells expressing the fusion protein CFP (cyan fluorescent protein)-dynamitin or CFP-MB (the MacMA

171 tein kinase A (PKA) consisting of fusions of cyan fluorescent protein, a phosphoamino acid binding do

172 nced yellow fluorescent protein and enhanced cyan fluorescent protein, allowing detection of zinc-ind

173 ore, the septin Cdc12p, fused with yellow or cyan fluorescent protein, also colocalized with Myo1p an

174 on of calmodulin within Citrine or fusion of cyan fluorescent protein, calmodulin, a calmodulin-bindi

175 ed from phagocytic cups earlier than did p85-cyan fluorescent protein, indicating that SHIP-1 inhibit

176 cts were used to express SNAP-25 tagged with cyan fluorescent protein, VAMP-2 tagged with yellow fluo

177 uorescence resonance energy transfer between cyan fluorescent protein- and yellow fluorescent protein

178 nce resonance energy transfer (FRET) between cyan fluorescent protein- and yellow fluorescent protein

179 e resonance energy transfer between enhanced cyan fluorescent protein-CaM and Na(V)1.5(4X) channels t

180 mouse liver sections after co-expression of cyan fluorescent protein-CCRP and yellow fluorescent pro

181 Selective immobilization of cyan fluorescent protein-D2 receptors (C-D2Rs) in the pl

182 refrontal cortex (PFC) neurons from enhanced cyan fluorescent protein-expressing mice.

183 uorescence resonance energy transfer between cyan fluorescent protein-fused and yellow fluorescent pr

184 tween Gialpha-yellow fluorescent protein and cyan fluorescent protein-Gbeta chimeras in HeLa cells.

185 al microscopy of coexpressed YFP-hGRbeta and cyan fluorescent protein-hGRalpha in COS-1 cells indicat

186 ne the stability of complexes formed between cyan fluorescent protein-labeled alpha(2A)-adrenorecepto

187 fluorescent protein (YFP) move along tubulin-cyan fluorescent protein-labeled microtubules in respons

188 The targeting of these enhanced cyan fluorescent protein-lac repressor-tagged RARalpha-c

189 We show that an enhanced cyan fluorescent protein-M. tuberculosis CrgA (ECFP-CrgA

190 Enhanced cyan fluorescent protein-Munc18-1 and a citrine variant

191 In addition, enhanced cyan fluorescent protein-Munc18-1 and a citrine variant

192 low fluorescent protein (EYFP)- and enhanced cyan fluorescent protein-NHPX fusions, we show here that

193 FRET between cyan fluorescent protein-PLB and yellow fluorescent prot

194 ciation with SERCA, measured by FRET between cyan fluorescent protein-SERCA and yellow fluorescent pr

195 escent protein-tagged (EYFP) GluCl alpha and cyan fluorescent protein-tagged (ECFP) GluCl beta.

196 sonance energy transfer was detected between cyan fluorescent protein-tagged DAT and yellow fluoresce

197 The cyan fluorescent protein-tagged Galpha and yellow fluore

198 Co-expression of hemagglutinin-tagged and cyan fluorescent protein-tagged UGT1A proteins, followed

199 escent protein with the chloride-insensitive cyan fluorescent protein.

200 tro assays and cells overexpressing stathmin-cyan fluorescent protein.

201 nsgenic mice in which red, green, yellow, or cyan fluorescent proteins (together termed XFPs) were se

202 his upregulation, we incorporated yellow and cyan fluorescent proteins (YFPs and CFPs) into the alpha

203 icistronic mRNAs encoding enhanced green and cyan fluorescent proteins as the first and second cistro

204 ergy transfer (FRET) acceptor for the newest cyan fluorescent proteins.

205 was generated in transgenic mice carrying a cyan fluorescent reporter protein (CFP) gene linked to t

206 The crystal structure of the cyan-fluorescent Cerulean green fluorescent protein (GFP

207 thesis, cDNA constructs were created to fuse cyan-fluorescent protein (CFP) to the N terminus of SERC

208 outer membrane protein A (OmpA) fused to the cyan-fluorescent protein AmCyan.

209 Transplantation of cyan-fluorescent-protein-expressing haematopoietic stem

210 the reversible intermolecular association of cyan-GFP-labelled calmodulin with yellow-GFP-labelled M1

211 The blue or cyan GFPs have the disadvantages of less brightness and

212 responsive to excitation light used to image cyan, green, or red fluorescent protein variants, allowi

213 ted by endogenous biomolecules compared with cyan, green, yellow, and orange FPs.

214 ur spectrally distinct fluorescent proteins (cyan, green, yellow, and red) that are fused to a transc

215 grouped into four color classes by emission, cyan, green, yellow, and red.

216 a sharp detection border was present in the cyan/green spectral region.

217 P538-K66M suggest that natural selection for cyan is an exquisitely fine-tuned and highly cooperative

218 are red shifted to 455 and 460 nm, emitting cyan light and green light, respectively.

219 meric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light.

220 gineered, orange-red FP that is excitable by cyan light.

221 lution for automated scoring of affinity and cyan-magenta-yellow-key (CMYK) color-coding for scoring

222 o express and compare six FPs (blue mTagBFP, cyan mCerulean, green CrGFP, yellow Venus, orange tdToma

223 Fluorescence was excited with cyan or blue LEDs on alternating camera frames, thus pro

224 cells as chimeric proteins fused to enhanced cyan or yellow fluorescent protein (CFP or YFP, respecti

225 FtsZ, FtsA, FtsQ, FtsL and FtsI to enhanced cyan or yellow fluorescent protein (ECFP or EYFP respect

226 f functional Ctr1 monomers fused with either cyan or yellow fluorescent protein resulted in fluoresce

227 receptors and G protein subunits tagged with cyan or yellow fluorescent protein showed that receptors

228 uorescence resonance energy transfer between cyan or yellow fluorescent protein-labeled G protein sub

229 Coexpression of receptors tagged with the cyan or yellow fluorescent proteins (CFP or YFP) resulte

230 HEK293 cells coexpressing IL-17RA fused to cyan or yellow fluorescent proteins (CFP or YFP) were us

231 genes corresponding to the N termini of the cyan or yellow fluorescent proteins were fused to the en

232 alpha(1C)- and beta-subunits were fused with cyan or yellow fluorescent proteins, and functionally co

233 eceptor constructs tagged with luciferase or cyan or yellow fluorescent proteins.

234 opsin1 and found that it absorbs in the blue-cyan range of the light spectrum.

235 olouration efficiency at the band edge (blue-cyan region) are 4.8x10(6) m(-1) and 190 cm(2) C(-1), re

236 from the genome and expression of yellow or cyan reporters is enabled.

237 mera of native PKCdelta fused to yellow- and cyan-shifted green fluorescent protein, which can be exp

238 y transfer (FRET) and increased the ratio of cyan to yellow emissions by up to 1.5-fold with apparent

239 f PSmOrange enable its simultaneous use with cyan-to-green photoswitchable proteins to study four int

240 MP decreased FRET and increased the ratio of cyan-to-yellow emissions by 10-30% in living mammalian c

241 The open fractures (vug) are colored cyan whereas the calcite-filled fractures (high density

242 sses between the fluorescent labels, such as cyan, yellow and monomeric red fluorescent proteins.

243 different fluorescent proteins (FP) (green, cyan, yellow or red FPs) in two different binary plasmid

244 es, and arrival at the plasma membrane using cyan/yellow fluorescent protein-tagged glycosylphosphati

245 PRV-263 genome and conditional expression of cyan/yellow reporters.