ライフサイエンスコーパス: cyanogen

1 f bovine insulin was also investigated using cyanogen.

2 cyanide formed after enzymatic hydrolysis of cyanogens.

3 o investigated for maximum recovery of total cyanogens.

4 n used in transgenic cassava to reduce toxic cyanogens.

5 Trace organic species, including cyanogen and ethane, were found in surface measurements.

6 Because cyanogens and minor metabolites of cyanide have not indu

7 verted to covalent links by ethanedinitrile (cyanogen) and identified using mass spectrometry.

8 0)), and three were generated against larger cyanogen bromide (A) alpha chain derivatives with each e

9 ed metabolites including the natural product cyanogen bromide (BrCN), which exhibits pronounced allel

10 on of oligodeoxyribonucleotides (ODNs) using cyanogen bromide (BrCN).

11 Electrophoresis of a cyanogen bromide (CNBr) (CB) digest of sternal cartilage

12 We have previously shown that cyanogen bromide (CNBr) cleavage of cornified envelopes

13 chemokine were identified by MALDI-MS of its cyanogen bromide (CNBr) cleavage products.

14 homogenized in formic acid and subjected to cyanogen bromide (CNBr) cleavage, and the pattern of che

15 is, mass spectral analysis of the C-terminal cyanogen bromide (CNBr) fragment, and comparison of reco

16 Cyanogen bromide (CNBr) is a common chemical used to hyd

17 raphy or SDS-PAGE followed by digestion with cyanogen bromide (CNBr) or trypsin.

18 First, ADH was bound to GAMP activated with cyanogen bromide (CNBr) or with 1-cyano-4(dimethylamino)

19 toxified polysaccharides were activated with cyanogen bromide (CNBr) or with 1-cyano-4-dimethylaminop

20 abeled sigma-1 receptor using Endo Lys C and cyanogen bromide (CNBr) revealed that the [ (125)I]-N-IA

21 rypsin fragments and further localized using cyanogen bromide (CNBr), which hydrolyzes proteins on th

22 ligand and L-tyrosine as the spacer arm to a cyanogen bromide (CNBr)-activated-Sepharose 4B matrix.

23 ced/alkylated, delipidated, and cleaved with cyanogen bromide (CNBr).

24 palmitoylated alpha-tubulin was cleaved with cyanogen bromide (CNBr).

25 gradient centrifugation and was cleaved with cyanogen bromide (CNBr).

26 specificity, using peptides derived from the cyanogen bromide 11 (CB11) fragment of CII.

27 Cyanogen bromide activation was transient relative to ol

28 ase I was immobilized onto agarose beads via cyanogen bromide activation.

29 Cyanogen bromide also gives high cleavage yields and int

30 on of full-length aptamer in the presence of cyanogen bromide and a 5.9- to 7.6-fold enhancement in t

31 iments reported here, we cleaved VV ATI with cyanogen bromide and determined that the myristoyl moiet

32 lenediamines via successive cyclization with cyanogen bromide and diazotization in the presence of an

33 addition, these proteins were digested with cyanogen bromide and peptide mapping by LC-MS was establ

34 Edman degradation sequencing of radiolabeled cyanogen bromide and skatole digestion products that wer

35 eatment of the 60 kDa cleavage fragment with cyanogen bromide and subsequent MALDI-TOF analysis of th

36 Cleavage of 2-BDB-TAMP-modified enzyme with cyanogen bromide and subsequent separation of peptides b

37 ent, NTS1 was digested with a combination of cyanogen bromide and trypsin and/or chymotrypsin.

38 Cyanogen bromide cleavage analyses of the altered pp60(c

39 Cyanogen bromide cleavage analysis suggests that one or

40 ing region from each tubulin was obtained by cyanogen bromide cleavage and identified by mass spectro

41 Mapping of the site of modification by cyanogen bromide cleavage and peptide sequencing has sho

42 ansmembrane 1 was labeled, as established by cyanogen bromide cleavage and separation by gel and/or h

43 Cyanogen bromide cleavage demonstrated its covalent atta

44 peptides of InlA-MH(6)-Cws were obtained by cyanogen bromide cleavage followed by purification on a

45 rometry and tandem mass spectrometry) of the cyanogen bromide cleavage fragments of the C26A and C15A

46 dues by endoproteinase Lys-C cleavage and by cyanogen bromide cleavage of another receptor construct

47 In addition, cyanogen bromide cleavage of bovine gamma-glutamyl carbo

48 Cyanogen bromide cleavage of the 32P-labeled rat PDE4D3

49 Cyanogen bromide cleavage of the [125I]IACoc photolabele

50 Cyanogen bromide cleavage of the covalently labeled rece

51 Cyanogen bromide cleavage of the phosphorylated beta 1 A

52 or 23-mer triphosphorylated RNA, followed by cyanogen bromide cleavage of the photo-linked enzyme, lo

53 mployed here a novel solid-phase approach to cyanogen bromide cleavage of the photolabeled receptor f

54 Cyanogen bromide cleavage of the precursor protein, foll

55 Cyanogen bromide cleavage of the receptor yielded a majo

56 Cyanogen bromide cleavage of this molecule yielded a sin

57 To examine the bridging, the cyanogen bromide cleavage products of mutacin II and its

58 ced at fixed positions in Sup(1-61) to allow cyanogen bromide cleavage to facilitate subsequent mass

59 Amino acid sequencing after cyanogen bromide cleavage yielded two sequences that are

60 s achieved by purification, deglycosylation, cyanogen bromide cleavage, and sequencing of labeled wil

61 After cyanogen bromide cleavage, His(119) is predicted to be w

62 of several analytical techniques, including cyanogen bromide cleavage, reversed-phase chromatography

63 2 with Met to generate an additional site of cyanogen bromide cleavage, the labeled fragment was redu

64 n comparison to recombinant Tromp1 following cyanogen bromide cleavage, which further confirmed the i

65 ls, with the labeled domain then released by cyanogen bromide cleavage.

66 Furthermore, cyanogen bromide cleaved a series of wild type and mutan

67 Cyanogen bromide cleaved proHNP-1(20-94) at the fortuito

68 y cross-linked to DNA substrates, to partial cyanogen bromide degradation or trypsin proteolysis in o

69 cation, followed by sequencing of an in situ cyanogen bromide digest of membrane bound Hrp12, yielded

70 Microsequencing of peptide fragments from a cyanogen bromide digest of p78 identified this protein a

71 Cyanogen bromide digestion analysis of the cross-linked

72 Mixed peptide sequencing following cyanogen bromide digestion identified the 70-kDa membran

73 Cyanogen bromide digestion of p66shc produced a phosphor

74 Cyanogen bromide digestion of the covalent TnI-TnC compl

75 uding resistance to proteases, resistance to cyanogen bromide digestion, and an ability to form amylo

76 were analyzed by mass spectrometry following cyanogen bromide digestion, and the identity and relativ

77 Cyanogen bromide digestion, immunoaffinity chromatograph

78 nteractions that are sensitive to reduction, cyanogen bromide digestion, or limited acid digestion.

79 try of peptides generated by proteolytic and cyanogen bromide digestion.

80 and subjected matrix fibronectin to acid or cyanogen bromide digestion.

81 When cyanogen bromide digests of matrix 125I-fibronectin and

82 peptides resulting from complete tryptic and cyanogen bromide digests of the latent protease chain of

83 During the chromatographic separation of cyanogen bromide digests, observation of the absorbance

84 se F, followed by cyanogen bromide; Route B, cyanogen bromide followed by endo-N-glycosydase F.

85 the cytochrome from wild-type bacteria with cyanogen bromide followed by trypsin were analysed by on

86 The tolerogenic epitope is contained in cyanogen bromide fragment 11 (CB11) and is structurally

87 A change in mobility for an N-terminal cyanogen bromide fragment accompanied disulfide cross-li

88 rine phosphorylation is thought to be in the cyanogen bromide fragment containing residues 2-65.

89 III collagen and 9 peptides derived from the cyanogen bromide fragment of bovine type III collagen, a

90 The labeled domain was within the cyanogen bromide fragment of the receptor including the

91 en localized to arginine residues within the cyanogen bromide fragment-(341-380) that contains the pr

92 tein encoded by this sequence, its predicted cyanogen bromide fragmentation, and calculated isoelectr

93 hod that quickly and reliably identifies the cyanogen bromide fragments and posttranslational modific

94 unique peptide determinants contained within cyanogen bromide fragments CB10 and CB11 showing the sig

95 ed within the negatively charged, C-terminal cyanogen bromide fragments of alpha- and beta-tubulin su

96 Microsequencing of cyanogen bromide fragments of purified icIL-1RaII provid

97 duct of a single probe molecule with the two cyanogen bromide fragments of the CCK receptor represent

98 hat amino acids within the N- and C-terminal cyanogen bromide fragments of the motor domain formed cr

99 ed to 15 synthetic peptides representing all cyanogen bromide fragments.

100 rformance liquid chromatography, tryptic and cyanogen bromide hydrolysis, amino acid analysis, and ma

101 ed wild-type and methionine mutant DATs with cyanogen bromide identified the sequence between residue

102 Cleavage with cyanogen bromide is beneficial for analysis of membrane

103 In one approach, cyanogen bromide is used to cleave relatively large pept

104 n of residues flanking this site followed by cyanogen bromide mapping of the [(125)I]RTI 82-labeled m

105 Using [(32)P]Glc-6-P coupled with cyanogen bromide mapping, we demonstrated that the phosp

106 residues from the C-terminal end within the cyanogen bromide peptide CB6.

107 A cyanogen bromide peptide containing Glu-14 allows the ex

108 This region is located within the cyanogen bromide peptide fragment alpha1(I) CB6 and is a

109 A large cyanogen bromide peptide was recovered, and its further

110 -terminal amino acid analysis of KAM and its cyanogen bromide peptides firmly correlated its amino ac

111 Mass spectral analysis of cyanogen bromide peptides has established that the cysti

112 Analysis of cyanogen bromide peptides of each 3D preparation showed

113 The cyanogen bromide peptides were isolated and characterize

114 d monomeric receptor bands were cleaved with cyanogen bromide to demonstrate that both of the photola

115 ing ligands also accelerated the addition of cyanogen bromide to these complexes but slowed the addit

116 e of OmpA fragments generated by protease or cyanogen bromide treatment and by competitive inhibition

117 -51, but its fragmentation pattern following cyanogen bromide treatment is incompatible with the line

118 using a metal ion affinity column, and after cyanogen bromide treatment, avidin affinity purification

119 These two forms were cleaved with cyanogen bromide, and both yielded 40-kDa fragments that

120 endoproteinase Glu-C, endoproteinase Lys-C, cyanogen bromide, and hydroxylamine were consistent with

121 te to allow selective chemical cleavage with cyanogen bromide, and rHD-5 was used to elicit polyclona

122 isolated from each section and cleaved with cyanogen bromide, and the peptides were separated and an

123 rotein bands were individually digested with cyanogen bromide, and the resulting peptide fragments we

124 beta-tubulin was digested with formic acid, cyanogen bromide, endoproteinase Glu-C, or endoproteinas

125 inea pig sigma-1 receptor with EndoLys-C and cyanogen bromide, the [(125)I]IAF label was identified b

126 ated [(32)P]phosphate-G6Pase intermediate by cyanogen bromide, the [(32)P]phosphate remains bound to

127 In some experiments, a cyanogen bromide-activated resin column bound with CAT w

128 cid sequence is provided, was crosslinked to cyanogen bromide-activated Sepharose 4B and used to immu

129 lumn (containing the GAATTC motif coupled to cyanogen bromide-activated Sepharose 4B) binds EcoRI in

130 pressor, and Gal4 were chemically coupled to cyanogen bromide-activated Sepharose and the temperature

131 Incubation of denatured and cyanogen bromide-cleaved type XI collagen with radiolabe

132 consistent with both N-terminal and internal cyanogen bromide-derived amino acid sequence.

133 Further proteolysis of the cyanogen bromide-generated fragment containing cysteine

134 Amino acid microsequencing of two cyanogen bromide-generated peptide fragments of the 43-k

135 C-18-derivatized silica gel and cleaved with cyanogen bromide.

136 es of gC1(457t),the protein was cleaved with cyanogen bromide.

137 rp leader carrier protein was performed with cyanogen bromide.

138 sequential in-gel digestion with trypsin and cyanogen bromide.

139 ment was generated by chemical cleavage with cyanogen bromide.

140 se C, then endo-N-glycosidase F, followed by cyanogen bromide; Route B, cyanogen bromide followed by

141 elty of this approach is the ease with which cyanogen can be administered to a protein sample and the

142 rmation and decomposition of chloramines and cyanogen chloride (CNCl) were measured for a range of ch

143 dioxide, sulfur dioxide, hydrochloric acid, cyanogen chloride, and hydrogen cyanide in negative pola

144 hylene oxide, sulfur dioxide, acrylonitrile, cyanogen chloride, hydrogen cyanide, acrolein, formaldeh

145 n SH-functional group; CO2, SO2, and perhaps cyanogen [(CN)2] may be present within the surface mater

146 When cyanogen concentrations were lower, the resulted cyanide

147 Total cyanogen content in plum and almond kernels, as well as

148 A method was developed incorporating cyanogen, enzymatic digestion, one-dimensional gel elect

149 e salt bridge, paired group-specific reagent cyanogen (ethanedinitrile; C(2)N(2)) converts naturally

150 A mild method was developed to remove cyanogens from cassava leaves that involved three consec

151 The traditional method for removing cyanogens from pounded cassava leaves is by boiling in w

152 A cyanogen gas production rate of (1.2 +/- 0.3) x10(26) mo

153 6.45 astronomical units showed emission from cyanogen gas.

154 f a phosphorescence-based sensing system for cyanogen halides.

155 ves is by boiling in water which removed all cyanogens in 10 min.

156 uantification of total cyanogenic compounds (cyanogens) in plants was studied using a novel ninhydrin

157 crease root protein levels result in reduced cyanogen levels in roots.

158 The cyanogen-linked proteins were isolated by polyacrylamide

159 Peptides resulting from cyanogen-mediated intermolecular cross-linking of HEW ly

160 Gas-phase reaction between copper vapor and cyanogen (NCCN) clearly gives CuCN (not CuNC).

161 s product, cyanoacetaldehyde), with cyanate, cyanogen, or urea.

162 measuring ROS accumulation in transgenic low-cyanogen plants with and without cyanide complementation

163 We demonstrate that cyanogens play a central role in cassava nitrogen metabo

164 Cyanogen readily permeates cell walls and membranes.

165 The spatial distribution of cyanogen was considerably more diffuse and extended comp

166 oestrogens, flavonoids, simple phenolics and cyanogens with higher apparent affinities (K(m)) and spe