ライフサイエンスコーパス: cyanogen bromide

1 C-18-derivatized silica gel and cleaved with cyanogen bromide.

2 es of gC1(457t),the protein was cleaved with cyanogen bromide.

3 rp leader carrier protein was performed with cyanogen bromide.

4 sequential in-gel digestion with trypsin and cyanogen bromide.

5 ment was generated by chemical cleavage with cyanogen bromide.

6 specificity, using peptides derived from the cyanogen bromide 11 (CB11) fragment of CII.

7 0)), and three were generated against larger cyanogen bromide (A) alpha chain derivatives with each e

8 In some experiments, a cyanogen bromide-activated resin column bound with CAT w

9 cid sequence is provided, was crosslinked to cyanogen bromide-activated Sepharose 4B and used to immu

10 lumn (containing the GAATTC motif coupled to cyanogen bromide-activated Sepharose 4B) binds EcoRI in

11 pressor, and Gal4 were chemically coupled to cyanogen bromide-activated Sepharose and the temperature

12 Cyanogen bromide activation was transient relative to ol

13 ase I was immobilized onto agarose beads via cyanogen bromide activation.

14 Cyanogen bromide also gives high cleavage yields and int

15 on of full-length aptamer in the presence of cyanogen bromide and a 5.9- to 7.6-fold enhancement in t

16 iments reported here, we cleaved VV ATI with cyanogen bromide and determined that the myristoyl moiet

17 lenediamines via successive cyclization with cyanogen bromide and diazotization in the presence of an

18 addition, these proteins were digested with cyanogen bromide and peptide mapping by LC-MS was establ

19 Edman degradation sequencing of radiolabeled cyanogen bromide and skatole digestion products that wer

20 eatment of the 60 kDa cleavage fragment with cyanogen bromide and subsequent MALDI-TOF analysis of th

21 Cleavage of 2-BDB-TAMP-modified enzyme with cyanogen bromide and subsequent separation of peptides b

22 ent, NTS1 was digested with a combination of cyanogen bromide and trypsin and/or chymotrypsin.

23 These two forms were cleaved with cyanogen bromide, and both yielded 40-kDa fragments that

24 endoproteinase Glu-C, endoproteinase Lys-C, cyanogen bromide, and hydroxylamine were consistent with

25 te to allow selective chemical cleavage with cyanogen bromide, and rHD-5 was used to elicit polyclona

26 isolated from each section and cleaved with cyanogen bromide, and the peptides were separated and an

27 rotein bands were individually digested with cyanogen bromide, and the resulting peptide fragments we

28 ed metabolites including the natural product cyanogen bromide (BrCN), which exhibits pronounced allel

29 on of oligodeoxyribonucleotides (ODNs) using cyanogen bromide (BrCN).

30 Cyanogen bromide cleavage analyses of the altered pp60(c

31 Cyanogen bromide cleavage analysis suggests that one or

32 ing region from each tubulin was obtained by cyanogen bromide cleavage and identified by mass spectro

33 Mapping of the site of modification by cyanogen bromide cleavage and peptide sequencing has sho

34 ansmembrane 1 was labeled, as established by cyanogen bromide cleavage and separation by gel and/or h

35 Cyanogen bromide cleavage demonstrated its covalent atta

36 peptides of InlA-MH(6)-Cws were obtained by cyanogen bromide cleavage followed by purification on a

37 rometry and tandem mass spectrometry) of the cyanogen bromide cleavage fragments of the C26A and C15A

38 dues by endoproteinase Lys-C cleavage and by cyanogen bromide cleavage of another receptor construct

39 In addition, cyanogen bromide cleavage of bovine gamma-glutamyl carbo

40 Cyanogen bromide cleavage of the 32P-labeled rat PDE4D3

41 Cyanogen bromide cleavage of the [125I]IACoc photolabele

42 Cyanogen bromide cleavage of the covalently labeled rece

43 Cyanogen bromide cleavage of the phosphorylated beta 1 A

44 or 23-mer triphosphorylated RNA, followed by cyanogen bromide cleavage of the photo-linked enzyme, lo

45 mployed here a novel solid-phase approach to cyanogen bromide cleavage of the photolabeled receptor f

46 Cyanogen bromide cleavage of the precursor protein, foll

47 Cyanogen bromide cleavage of the receptor yielded a majo

48 Cyanogen bromide cleavage of this molecule yielded a sin

49 To examine the bridging, the cyanogen bromide cleavage products of mutacin II and its

50 ced at fixed positions in Sup(1-61) to allow cyanogen bromide cleavage to facilitate subsequent mass

51 Amino acid sequencing after cyanogen bromide cleavage yielded two sequences that are

52 s achieved by purification, deglycosylation, cyanogen bromide cleavage, and sequencing of labeled wil

53 After cyanogen bromide cleavage, His(119) is predicted to be w

54 of several analytical techniques, including cyanogen bromide cleavage, reversed-phase chromatography

55 2 with Met to generate an additional site of cyanogen bromide cleavage, the labeled fragment was redu

56 n comparison to recombinant Tromp1 following cyanogen bromide cleavage, which further confirmed the i

57 ls, with the labeled domain then released by cyanogen bromide cleavage.

58 Furthermore, cyanogen bromide cleaved a series of wild type and mutan

59 Cyanogen bromide cleaved proHNP-1(20-94) at the fortuito

60 Incubation of denatured and cyanogen bromide-cleaved type XI collagen with radiolabe

61 Electrophoresis of a cyanogen bromide (CNBr) (CB) digest of sternal cartilage

62 We have previously shown that cyanogen bromide (CNBr) cleavage of cornified envelopes

63 chemokine were identified by MALDI-MS of its cyanogen bromide (CNBr) cleavage products.

64 homogenized in formic acid and subjected to cyanogen bromide (CNBr) cleavage, and the pattern of che

65 is, mass spectral analysis of the C-terminal cyanogen bromide (CNBr) fragment, and comparison of reco

66 Cyanogen bromide (CNBr) is a common chemical used to hyd

67 raphy or SDS-PAGE followed by digestion with cyanogen bromide (CNBr) or trypsin.

68 First, ADH was bound to GAMP activated with cyanogen bromide (CNBr) or with 1-cyano-4(dimethylamino)

69 toxified polysaccharides were activated with cyanogen bromide (CNBr) or with 1-cyano-4-dimethylaminop

70 abeled sigma-1 receptor using Endo Lys C and cyanogen bromide (CNBr) revealed that the [ (125)I]-N-IA

71 rypsin fragments and further localized using cyanogen bromide (CNBr), which hydrolyzes proteins on th

72 ligand and L-tyrosine as the spacer arm to a cyanogen bromide (CNBr)-activated-Sepharose 4B matrix.

73 palmitoylated alpha-tubulin was cleaved with cyanogen bromide (CNBr).

74 gradient centrifugation and was cleaved with cyanogen bromide (CNBr).

75 ced/alkylated, delipidated, and cleaved with cyanogen bromide (CNBr).

76 y cross-linked to DNA substrates, to partial cyanogen bromide degradation or trypsin proteolysis in o

77 consistent with both N-terminal and internal cyanogen bromide-derived amino acid sequence.

78 cation, followed by sequencing of an in situ cyanogen bromide digest of membrane bound Hrp12, yielded

79 Microsequencing of peptide fragments from a cyanogen bromide digest of p78 identified this protein a

80 Cyanogen bromide digestion analysis of the cross-linked

81 Mixed peptide sequencing following cyanogen bromide digestion identified the 70-kDa membran

82 Cyanogen bromide digestion of p66shc produced a phosphor

83 Cyanogen bromide digestion of the covalent TnI-TnC compl

84 uding resistance to proteases, resistance to cyanogen bromide digestion, and an ability to form amylo

85 were analyzed by mass spectrometry following cyanogen bromide digestion, and the identity and relativ

86 Cyanogen bromide digestion, immunoaffinity chromatograph

87 nteractions that are sensitive to reduction, cyanogen bromide digestion, or limited acid digestion.

88 try of peptides generated by proteolytic and cyanogen bromide digestion.

89 and subjected matrix fibronectin to acid or cyanogen bromide digestion.

90 When cyanogen bromide digests of matrix 125I-fibronectin and

91 peptides resulting from complete tryptic and cyanogen bromide digests of the latent protease chain of

92 During the chromatographic separation of cyanogen bromide digests, observation of the absorbance

93 beta-tubulin was digested with formic acid, cyanogen bromide, endoproteinase Glu-C, or endoproteinas

94 se F, followed by cyanogen bromide; Route B, cyanogen bromide followed by endo-N-glycosydase F.

95 the cytochrome from wild-type bacteria with cyanogen bromide followed by trypsin were analysed by on

96 The tolerogenic epitope is contained in cyanogen bromide fragment 11 (CB11) and is structurally

97 A change in mobility for an N-terminal cyanogen bromide fragment accompanied disulfide cross-li

98 rine phosphorylation is thought to be in the cyanogen bromide fragment containing residues 2-65.

99 III collagen and 9 peptides derived from the cyanogen bromide fragment of bovine type III collagen, a

100 The labeled domain was within the cyanogen bromide fragment of the receptor including the

101 en localized to arginine residues within the cyanogen bromide fragment-(341-380) that contains the pr

102 tein encoded by this sequence, its predicted cyanogen bromide fragmentation, and calculated isoelectr

103 hod that quickly and reliably identifies the cyanogen bromide fragments and posttranslational modific

104 unique peptide determinants contained within cyanogen bromide fragments CB10 and CB11 showing the sig

105 ed within the negatively charged, C-terminal cyanogen bromide fragments of alpha- and beta-tubulin su

106 Microsequencing of cyanogen bromide fragments of purified icIL-1RaII provid

107 duct of a single probe molecule with the two cyanogen bromide fragments of the CCK receptor represent

108 hat amino acids within the N- and C-terminal cyanogen bromide fragments of the motor domain formed cr

109 ed to 15 synthetic peptides representing all cyanogen bromide fragments.

110 Further proteolysis of the cyanogen bromide-generated fragment containing cysteine

111 Amino acid microsequencing of two cyanogen bromide-generated peptide fragments of the 43-k

112 rformance liquid chromatography, tryptic and cyanogen bromide hydrolysis, amino acid analysis, and ma

113 ed wild-type and methionine mutant DATs with cyanogen bromide identified the sequence between residue

114 Cleavage with cyanogen bromide is beneficial for analysis of membrane

115 In one approach, cyanogen bromide is used to cleave relatively large pept

116 n of residues flanking this site followed by cyanogen bromide mapping of the [(125)I]RTI 82-labeled m

117 Using [(32)P]Glc-6-P coupled with cyanogen bromide mapping, we demonstrated that the phosp

118 residues from the C-terminal end within the cyanogen bromide peptide CB6.

119 A cyanogen bromide peptide containing Glu-14 allows the ex

120 This region is located within the cyanogen bromide peptide fragment alpha1(I) CB6 and is a

121 A large cyanogen bromide peptide was recovered, and its further

122 -terminal amino acid analysis of KAM and its cyanogen bromide peptides firmly correlated its amino ac

123 Mass spectral analysis of cyanogen bromide peptides has established that the cysti

124 Analysis of cyanogen bromide peptides of each 3D preparation showed

125 The cyanogen bromide peptides were isolated and characterize

126 se C, then endo-N-glycosidase F, followed by cyanogen bromide; Route B, cyanogen bromide followed by

127 inea pig sigma-1 receptor with EndoLys-C and cyanogen bromide, the [(125)I]IAF label was identified b

128 ated [(32)P]phosphate-G6Pase intermediate by cyanogen bromide, the [(32)P]phosphate remains bound to

129 d monomeric receptor bands were cleaved with cyanogen bromide to demonstrate that both of the photola

130 ing ligands also accelerated the addition of cyanogen bromide to these complexes but slowed the addit

131 e of OmpA fragments generated by protease or cyanogen bromide treatment and by competitive inhibition

132 -51, but its fragmentation pattern following cyanogen bromide treatment is incompatible with the line

133 using a metal ion affinity column, and after cyanogen bromide treatment, avidin affinity purification