ライフサイエンスコーパス: cyclohydrolase

1 of methylene tetrahydrofolate dehydrogenase/cyclohydrolase.

2 s appear distinct from any characterized IMP cyclohydrolase.

3 constituents confirms their origin as PR-AMP cyclohydrolase.

4 mino acid dopa decarboxylase (AADC), and GTP cyclohydrolase 1 (CH1) in a single transcription unit ha

5 Endothelium-targeted overexpression of GTP cyclohydrolase 1 (GCH), the rate limiting enzyme in BH4

6 We have studied the GTP-cyclohydrolase 1 (GCH-1) gene in 30 patients with the di

7 GTP cyclohydrolase 1 (GCH1) and its product tetrahydrobiopte

8 Here we report that reduction of cardiac GTP cyclohydrolase 1 (GCH1) degradation by genetic and pharm

9 GTP cyclohydrolase 1 (GCH1) is rate limiting in the provisio

10 rate limiting step for BH4 production is GTP Cyclohydrolase 1 (GCH1).

11 GTP-cyclohydrolase 1 (GTP-CH1) catalyzes the first step for

12 BH4, oxidised biopterins, GTP-cyclohydrolase 1 (GTPCH-1, the rate-limiting enzyme in B

13 ssociated viruses expressing human TH or GTP cyclohydrolase 1 (GTPCH1) were injected into the striatu

14 ant degradation of guanosine 5'-triphosphate cyclohydrolase 1 (GTPCH1) with consequent deficiency of

15 eno-associated viruses expressing TH and GTP cyclohydrolase 1 (GTPCH1).

16 n (a BH4 precursor) or overexpression of GTP cyclohydrolase 1 (the rate-limiting enzyme for BH4 biosy

17 ies have revealed an association between GTP cyclohydrolase 1 polymorphisms, which decrease tetrahydr

18 GTP cyclohydrolase 1, encoded by the GCH1 gene, is an essent

19 BH4 is regulated by GTP cyclohydrolase 1, the rate-limiting enzyme in BH4 biosyn

20 of this biopterin increases with age in GTP cyclohydrolase 1-deficient hyperphenylalaninemia-1 (hph-

21 in the gene encoding guanosine triphosphate cyclohydrolase 1.

22 r BH(4) production is guanosine triphosphate cyclohydrolase-1 (GTPCH-1).

23 ference RNA (siRNA)-mediated "knockdown" GTP cyclohydrolase-1 (GTPCH1), the rate-limiting enzyme in B

24 biosynthetic enzymes (guanosine triphosphate cyclohydrolase-1 and dihydrofolate reductase).

25 e expression of an unregulated bacterial GTP cyclohydrolase-1 in plants would increase pterin biosynt

26 The expression of bacterial GTP cyclohydrolase-1 in transgenic Arabidopsis resulted in a

27 The folE gene encoding GTP cyclohydrolase-1 was cloned from Escherichia coli and in

28 We postulated that GTP cyclohydrolase-1, which catalyzes the first committed st

29 ally methylenetetrahydrofolate dehydrogenase-cyclohydrolase 2 (MTHFD2), emerged as a top candidate in

30 e (FTL) and/or 5,10-methenyltetrahydrofolate cyclohydrolase/5,10-methylene tetrahydrofolate dehydroge

31 otide, between the formyltransferase and the cyclohydrolase active sites.

32 osynthesis, the AICAR transformylase and IMP cyclohydrolase activities of the bifunctional enzyme ATI

33 ncompasses both AICAR transformylase and IMP cyclohydrolase activities that are responsible for the c

34 olate-dependent AICAR transformylase and IMP cyclohydrolase activities that catalyzes the last two st

35 ide transformylase and inosine monophosphate cyclohydrolase activities, and exist as homodimers based

36 d elevations in tyrosine hydroxylase and GTP cyclohydrolase activities.

37 CH2-THF dehydrogenase and 5,10-methenyl-THF cyclohydrolase activities.

38 e activity is co-located with a methenyl-THF cyclohydrolase activity as part of bifunctional or trifu

39 at Lys137 is responsible for the increase in cyclohydrolase activity for dimeric ATIC, which was repo

40 We previously reported that deficits in GTP cyclohydrolase activity in Drosophila heterozygous for m

41 The enzyme responsible for observed IMP cyclohydrolase activity in Methanococcus jannaschii was

42 ared to test the hypothesis that the lack of cyclohydrolase activity in yMTD was due to the substitut

43 resented here, a catalytic mechanism for the cyclohydrolase activity is postulated.

44 d for site-directed mutagenesis to study the cyclohydrolase activity of this bifunctional enzyme.

45 e activity requires dimerization whereas the cyclohydrolase activity only slightly prefers the dimeri

46 The pH dependence of the PR-AMP cyclohydrolase activity shows a single titration event i

47 tant retained dehydrogenase activity, but no cyclohydrolase activity was detected.

48 f the interaction include an increase in GTP cyclohydrolase activity, with concomitant protection fro

49 nism of this enzyme and its apparent lack of cyclohydrolase activity.

50 AR) synthase and inosine monophosphate (IMP) cyclohydrolase activity.

51 oxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase).

52 arboxamide ribonucleotide transformylase IMP cyclohydrolase, an enzyme not previously known to be reg

53 that regulate biopterin bioavailability, GTP cyclohydrolase and dihydrofolate reductase exhibited a c

54 nalogues and inhibitors suggest that the GTP cyclohydrolase and pyrophosphate phosphohydrolase activi

55 l protein ATIC (AICAR transformylase and IMP cyclohydrolase) and is responsible for catalyzing the pe

56 al methylene-THF dehydrogenase, methenyl-THF cyclohydrolase, and 10-formyl-THF synthetase activities.

57 10-formyl-THF synthetase, 5,10-methenyl-THF cyclohydrolase, and 5,10-methylene-THF dehydrogenase.

58 ggest that both tyrosine hydroxylase and GTP cyclohydrolase are induced in a coordinate and transcrip

59 tide formyltransferase/inosine monophosphate cyclohydrolase (ATIC) and thereby slows the metabolism o

60 de ribonucleotide (AICAR) transformylase/IMP cyclohydrolase (ATIC) is a bifunctional enzyme with fola

61 arboxamide ribonucleotide transformylase/IMP cyclohydrolase (ATIC) is a bifunctional protein possessi

62 leotide transformylase/inosine monophosphate cyclohydrolase (ATIC) is responsible for catalysis of th

63 leotide transformylase/inosine monophosphate cyclohydrolase (ATIC) is responsible for catalysis of th

64 oxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC), a bifunctional homodimeric enzyme

65 e AICAR transformylase/inosine monophosphate cyclohydrolase (ATIC).

66 Inosine 5'-monophosphate (IMP) cyclohydrolase catalyzes the cyclization of 5-formaminoi

67 exhibits sequence homology to the type I GTP cyclohydrolases characterized by FolE, but contrary to t

68 ibits significant homology to the type I GTP cyclohydrolases characterized by FolE.

69 ssible reactions with a palm fold to include cyclohydrolase chemistry.

70 Both the dehydrogenase/cyclohydrolase (D/C) domain and the synthetase domain co

71 antly inherited guanosine triphosphate (GTP)-cyclohydrolase deficiency, otherwise known as Segawa's d

72 biogenic amine and BH4 metabolism in the GTP cyclohydrolase deficient hph-1 mouse.

73 tead, it uses a new type of thermostable GTP cyclohydrolase enzyme that produces 2-amino-5-formylamin

74 recognizable homologues of the canonical GTP cyclohydrolase enzymes that are required for riboflavin

75 h is much faster than those of canonical GTP cyclohydrolase enzymes.

76 d-product BH(4) via interaction with the GTP cyclohydrolase feedback regulatory protein (GFRP).

77 olate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (MTHFD1

78 olate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (MTHFD1

79 olate dehydrogenase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase (MTHFD1

80 r the rate-limiting BH4 synthetic enzyme GTP cyclohydrolase (GCH) became undetectable in the sweat gl

81 Endothelium-targeted overexpression of GTP cyclohydrolase (GCH) I increased levels of the endotheli

82 GTP cyclohydrolase (GCH) III from Methanocaldococcus jannasc

83 GTP-cyclohydrolase (gch1), the first enzyme in this pathway,

84 We report that GTP cyclohydrolase (GCH1), the rate-limiting enzyme for tetr

85 ces the expression of guanosine triphosphate cyclohydrolase (GCH1), the rate-limiting enzyme in pteri

86 sduction with nitric oxide synthase with GTP cyclohydrolase genes.

87 ether AMPK suppresses the degradation of GTP-cyclohydrolase (GTPCH I), a key event in vascular endoth

88 synthesis is controlled enzymatically by GTP cyclohydrolase (GTPCH), we used GTPCH-depleted mice [hyp

89 ses H4B levels and enzymatic activity of GTP cyclohydrolase (GTPCH)-1, the first step of H4B biosynth

90 pain sensitivity and chronicity, and the GTP cyclohydrolase haplotype is a marker for these traits.

91 onofunctional Methanococcus vannielii PR-AMP cyclohydrolase has been developed, and the first charact

92 5'-Phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a centra

93 Similarly, a GTP cyclohydrolase I (fol2) mutant of yeast (Saccharomyces c

94 similar situation in Escherichia coli: a GTP cyclohydrolase I (folE) mutant, deficient in pterin synt

95 by targeted transgenic overexpression of GTP-cyclohydrolase I (GCH), prevented hypoxia-induced pulmon

96 c l-amino acid decarboxylase (AADC), and GTP cyclohydrolase I (GCH1) transcription; increases striata

97 B1), carbonyl reductase (CBR1 and CBR3), GTP-cyclohydrolase I (GCH1), and 6-pyruvoyltetrahydrobiopter

98 Overexpression of GTP cyclohydrolase I (GCH1), the rate-limiting enzyme for BH

99 GTP cyclohydrolase I (GCHI) mediates the first and committin

100 GTP cyclohydrolase I (GCYH-I) is an essential Zn(2+)-depende

101 GTP cyclohydrolase I (GCYH-I) is the first enzyme of the de

102 the first enzyme of the folate pathway, GTP cyclohydrolase I (GCYH-I), encoded in Escherichia coli b

103 GTP cyclohydrolase I (GTPCH I) is the rate-limiting enzyme f

104 fer of human guanosine 5'-triphosphate (GTP) cyclohydrolase I (GTPCH I), the first and rate-limiting

105 levels, in part through the induction of GTP cyclohydrolase I (GTPCH I), the rate-limiting enzyme for

106 5812 base pairs of rat GTP cyclohydrolase I (GTPCH) 5'-flanking region were cloned

107 Recently the GTP cyclohydrolase I (GTPCH) gene was isolated as the first

108 Inhibition of GTP cyclohydrolase I (GTPCH) has been used as a selective to

109 GTP cyclohydrolase I (GTPCH) is the rate-limiting enzyme for

110 ent degradation of guanosine 5'-triphosphate cyclohydrolase I (GTPCH), a rate-limiting enzyme in the

111 me in catecholamine (CA) biosynthesis of GTP cyclohydrolase I (GTPCH), rate-limiting enzyme in biosyn

112 selective and direct-acting inhibitor of GTP cyclohydrolase I (GTPCH), the first and rate-limiting en

113 ctor alpha (TNF-alpha) without affecting GTP cyclohydrolase I (GTPCH), the rate-limiting enzyme in th

114 Expression of both iNOS and GTP cyclohydrolase I (GTPCH), the rate-limiting enzyme in th

115 4), secondary to decreased expression of GTP cyclohydrolase I (GTPCH).

116 ey enzyme involved in BH(4) synthesis is GTP-cyclohydrolase I (GTPCH-I), which is stimulated by endot

117 s controlled by guanosine triphosphate (GTP) cyclohydrolase I (GTPCHI) and its feedback regulatory pr

118 Guanosine triphosphate cyclohydrolase I (GTPCHI) is a critical enzyme in catech

119 ro-d-neopterin triphosphate catalyzed by GTP cyclohydrolase I (GTPCHI).

120 Furthermore, expression of GTP cyclohydrolase I (the rate-limiting enzyme in de novo BH

121 uman tyrosine hydroxylase (hTH) or human GTP-cyclohydrolase I [GTPCHI, the rate-limiting enzyme for t

122 n (GFRP) mediates feedback inhibition of GTP cyclohydrolase I activity by tetrahydrobiopterin and als

123 , arginine decarboxylase gene activator, GTP cyclohydrolase I and a repressor of purine biosynthesis,

124 HPS, where activities of the key enzyme GTP-cyclohydrolase I are in the normal range, but total biop

125 for the quaternary structure of GFRP and GTP cyclohydrolase I complexes.

126 increased de novo synthesis for 6BH4 via GTP-cyclohydrolase I concomitant with high levels of 6BH4, a

127 ) and the activity of guanosine triphosphate cyclohydrolase I decreased in ihMCs exposed to HG and wa

128 II increased vascular guanosine triphosphate cyclohydrolase I expression and biopterin synthesis in p

129 Conversely, both switching off GTP cyclohydrolase I expression as well as inhibiting its en

130 GTP cyclohydrolase I feedback regulatory protein (GFRP) medi

131 ylalanine through complex formation with GTP cyclohydrolase I feedback regulatory protein (GFRP).

132 olved in 6BH4 biosynthesis/recycling and GTP-cyclohydrolase I feedback regulatory protein were expres

133 iopterin bioavailability by upregulating GTP-cyclohydrolase I gene expression and activity, resulting

134 and those additionally modified with the GTP cyclohydrolase I gene indicate that BH4 is critical for

135 with fibro-blasts possessing both TH and GTP cyclohydrolase I genes displayed biochemical restoration

136 To examine further the importance of GTP cyclohydrolase I in gene therapy for PD, in vivo micro-d

137 single enzyme, as is known to occur with GTP cyclohydrolase I in the Eucarya and Bacteria, but rather

138 The activity of GTP cyclohydrolase I is inhibited by (6R)-L-erythro-5,6,7,8-

139 estoration in a rat model of PD and that GTP cyclohydrolase I is sufficient for production of BH4.

140 s with Tet-regulated expression of human GTP cyclohydrolase I to regulate intracellular BH4 availabil

141 Because GFRP is a pentamer and GTP cyclohydrolase I was shown here by cross-linking experim

142 ne prevented the coordinate induction of GTP cyclohydrolase I with NOS2 after exposure to interleukin

143 used a synthetic gene based on mammalian GTP cyclohydrolase I, because this enzyme is predicted to es

144 ino-6-hydroxypyrimidine, an inhibitor of GTP cyclohydrolase I, decreased endothelium-dependent vasodi

145 nzyme in the cofactor synthesis pathway, GTP cyclohydrolase I, is activated by phosphorylation and in

146 ent increase of iNOS, guanosine triphosphate cyclohydrolase I, tetrahydrobiopterin, NO formation, and

147 line by fruit-specific overexpression of GTP cyclohydrolase I, the first enzyme of pteridine synthesi

148 o fruit up to 140-fold by overexpressing GTP cyclohydrolase I, the first enzyme of pteridine synthesi

149 ro data demonstrate that NAMDA inhibited GTP cyclohydrolase I, the rate-limiting enzyme for BH4 biosy

150 tracycline-regulated expression of human GTP cyclohydrolase I, the rate-limiting enzyme in BH4 synthe

151 (X haplotype) in the GCH1 gene, encoding GTP-cyclohydrolase I, the rate-limiting enzyme in biopterin

152 eric GFRP associate with one molecule of GTP cyclohydrolase I.

153 timulatory complexes is equal to that of GTP cyclohydrolase I.

154 the two outer faces of the torus-shaped GTP cyclohydrolase I.

155 tA (MJ0775 gene product), a new class of GTP cyclohydrolase I.

156 irst enzyme in its biosynthetic pathway, GTP cyclohydrolase I.

157 ignificant (>40%) amino acid identity to GTP cyclohydrolase II (GCH II), which catalyzes the committe

158 Three GTP cyclohydrolase II homologues in the Streptomyces coelico

159 s enzyme is different than the bacterial GTP cyclohydrolase II which catalyzes both reactions.

160 ene encoding a putative dual-functioning GTP cyclohydrolase II-3,4-dihydroxy-2-butanone-4-phosphate s

161 FLU encoding the dual-functional protein GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate s

162 this enzyme confirms the involvement of GTP cyclohydrolase III (ArfA) in archaeal riboflavin and Fo

163 This activity has been reported for a GTP cyclohydrolase III protein from Methanocaldococcus janna

164 posed to begin with an archaeal-specific GTP cyclohydrolase III that hydrolyzes the imidazole ring of

165 hese compounds were potent inhibitors of IMP cyclohydrolase (IMP CHase), a second activity of the bif

166 The inosine monophosphate cyclohydrolase (IMPCH) component (residues 1-199) of the

167 but not the NGF effect, NGF also induced GTP cyclohydrolase in a cAMP-dependent manner, while the EGF

168 Sphingosine induced GTP cyclohydrolase in a protein kinase C-independent manner

169 cetylcholine, which was inhibited by the GTP-cyclohydrolase inhibitor 2,4-diamino-6-hydroxypyrimidine

170 fase substrate AICAR, as well as with an IMP cyclohydrolase inhibitor, XMP, to 1.93 A resolution.

171 methylenetetrahydrofolate dehydrogenase and cyclohydrolase involved in one-carbon metabolism.

172 GTP cyclohydrolase is composed of a highly conserved homodec

173 thetase is encoded by the purP gene, and IMP cyclohydrolase is encoded by the purO gene.

174 he induction of tyrosine hydroxylase and GTP cyclohydrolase is not coordinately regulated.

175 Mammalian GTP cyclohydrolase is subject to end-product inhibition via

176 '-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a central enzyme in histidine biosynt

177 sine-5'-monophosphate cyclohydrolase (PR-AMP cyclohydrolase) is a Zn(2+) metalloprotein encoded by th

178 additionally modified with the gene for GTP cyclohydrolase l; an enzyme critical for BH4 synthesis.

179 thesis, QueF was proposed to be the putative cyclohydrolase-like enzyme responsible for this reaction

180 GTP is the precursor to queuosine and that a cyclohydrolase-like reaction was postulated as the initi

181 Genes encoding methenyl H(4)MPT cyclohydrolase (mch genes) were cloned and sequenced fro

182 n, we focus on the IMPCH active site and the cyclohydrolase mechanism through comparison of crystal s

183 oxamide ribonucleotide formyltransferase/IMP cyclohydrolase-mediated glucose transporter type 4 (GLUT

184 olate dehydrogenase/methenyltetrahydrofolate cyclohydrolase (MTHFD1).

185 '-phosphoribosyl) adenosine-5'-monophosphate cyclohydrolase (PR-AMP cyclohydrolase) is a Zn(2+) metal

186 Whereas canonical GTP cyclohydrolases produce this formylamino-pyrimidine nucl

187 boxamide ribonucleotideformyltransferase/IMP cyclohydrolase (PurH), an enzyme involved in de novo pur

188 noimidazolecarboxamide formyltransferase/IMP cyclohydrolase (PurH, EC 2.1.2.3/3.5.4.10).

189 Comparisons of the two types of IMP cyclohydrolase, PurO and PurH, revealed that there are n

190 an the forward rate (2.9 s(-1)), whereas the cyclohydrolase reaction is essentially unidirectional in

191 PurH but catalyzes a similar intramolecular cyclohydrolase reaction required for chromophore maturat

192 The cyclohydrolase reaction thus draws the overall bifunctio

193 THFD2 enzyme catalyzes the dehydrogenase and cyclohydrolase reactions, but the enzyme responsible for

194 leotide transformylase/inosine monophosphate cyclohydrolase, Steps 9 and 10), were studied in a polye

195 ethylene THF dehydrogenase/5,10-methenyl THF cyclohydrolase that acts upstream of 5-formyl THF format

196 re we report the identification of a new GTP cyclohydrolase that converts GTP to 7,8-dihydro-d-neopte

197 MptA is the archetype of a new class of GTP cyclohydrolases that catalyzes a series of reactions mos

198 Induction of GTP cyclohydrolase (the rate-limiting enzyme for the product

199 e activities of tyrosine hydroxylase and GTP cyclohydrolase, the rate-limiting enzymes in catecholami

200 ata, MTH1020 is confirmed as an archaeal IMP cyclohydrolase, thus designated as MthPurO.

201 0-methenyl tetrahydromethanopterin (H(4)MPT) cyclohydrolase, was constructed in vitro and recombined

202 release, and we found that the gene for GTP cyclohydrolase, which effectively regulates TH through s

203 Expression of GTP cyclohydrolase, which produces tetrahydrobiopterin (H(4)