ライフサイエンスコーパス: cysteine proteinase

1 en phytocystatins and papain, a prototypical cysteine proteinase.

2 ing of the RSL within the active site of the cysteine proteinase.

3 tocystatins are well-known inhibitors of C1A cysteine proteinases.

4 stic lamina by mobilizing tissue-destructive cysteine proteinases.

5 ties for target enzymes, vary with different cysteine proteinases.

6 ns are reversible, competitive inhibitors of cysteine proteinases.

7 en Ariel and with a fluorescent substrate of cysteine proteinases.

8 mino acids of the C1 (papain-like) family of cysteine proteinases.

9 pendent mechanism to inhibit both serine and cysteine proteinases.

10 like serine proteinases, but not papain-like cysteine proteinases.

11 at regulating a powerful array of lysosomal cysteine proteinases.

12 g bone resorption, distinct from that of the cysteine proteinases.

13 lls, but was ineffective against papain-like cysteine proteinases.

14 en shown to be effective against papain-like cysteine proteinases.

15 nd was abrogated by inhibitors of serine and cysteine proteinases.

16 oth chymotrypsin-like serine and papain-like cysteine proteinases.

17 ich is a member of the papain (C1) family of cysteine proteinases.

18 eactive centers to neutralize the serine and cysteine proteinases.

19 -mannosidase, as well as two other lysosomal cysteine proteinases.

20 inases-2 but not by inhibitors of serine and cysteine proteinases.

21 ty of SCCA1 and SCCA2 to inhibit papain-like cysteine proteinases.

22 tes or their released proteinases, including cysteine proteinase 1 (EhCP1), induce intestinal catheli

23 catalyzed in large part by a virally encoded cysteine proteinase (3Cpro) which is highly selective fo

24 ra, we have demonstrated that a unique 33-kD cysteine proteinase accumulates in the whorl in response

25 te that the I7L gene product and its encoded cysteine proteinase activity are responsible for vCPP ac

26 6 from the plant was correlated with loss of cysteine proteinase activity in the intestine thereby su

27 mediated by cysteine proteinases, and total cysteine proteinase activity patterns were identical bet

28 ing either toxopain-1 expression or specific cysteine proteinase activity significantly reduced conge

29 Gut cysteine proteinase activity was higher in beetles consu

30 s believed to be important for inhibition of cysteine proteinase activity.

31 n inhibit catS, as well as other papain-like cysteine proteinases, albeit at a rate 50-fold less than

32 /Zn SOD, adenosine-5'-phosphosulfate-kinase, cysteine proteinase and eIF(4G), thus confirming the inv

33 l antiviral agent against viruses encoding a cysteine proteinase and in particular may be an antiaden

34 virus I7L gene product is predicted to be a cysteine proteinase and is demonstrated in this study to

35 by the mir clones are similar to other known cysteine proteinases and are most closely related to the

36 Although both the cysteine proteinases and neutral collagenases participat

37 ganelle and provides a critical link between cysteine proteinases and parasite invasion for this clas

38 Cathepsin S is one of the most important cysteine proteinases and plays key roles in nematodes an

39 ers may be useful as active site titrants of cysteine proteinases and probes of their biological func

40 e human clade B serpins neutralize serine or cysteine proteinases and reside predominantly within the

41 radation of cathelicidins by amebic released cysteine proteinases and upregulation of proinflammatory

42 enzymes belonging to the serine proteinase, cysteine proteinase, and metallo-proteinase families.

43 Some of them are also lysosomal cysteine proteinases, and all bind to the GlcNAc-alpha-1

44 ts into the active site cleft of papain-like cysteine proteinases, and determine binding affinity.

45 s in a contact-dependent fashion mediated by cysteine proteinases, and total cysteine proteinase acti

46 In addition, treatment with MMP or cysteine proteinase antagonists significantly decreased

47 demonstrate for the first time that amoebic cysteine proteinases are a key virulence factor in amoeb

48 Though cysteine proteinases are acidophilic and normally confin

49 Because different cysteine proteinases are responsible for specific Ii deg

50 odontitis (GAgP), and gingipains, a group of cysteine proteinases, are critical virulence factors exp

51 is an aminopeptidase that shows homology to cysteine proteinases around the cysteine and histidine a

52 e P-8 complex 2 (P-8c2) consists of a 31-kDa cysteine proteinase associated with amastigote glycolipi

53 amily regulate a diverse array of serine and cysteine proteinases associated with essential biologica

54 The barley cysteine proteinase B (EPB) is the main protease respons

55 Bleomycin hydrolase (BH) is unusual among cysteine proteinases because it appears to form multihom

56 DMs exteriorize a complex mix of proteolytic cysteine proteinases, but maintain full elastinolytic po

57 ostly abolished by inhibitors for serine and cysteine proteinases, but not by those for MMPs and aspa

58 chymotrypsin-like serine and the papain-like cysteine proteinases by employing an RSL-dependent inhib

59 IL-1beta and IL-18 through activation of the cysteine proteinase caspase-1.

60 The cysteine proteinase cathepsin B has been implicated in t

61 at treatment of purified alphabetaI with the cysteine proteinase cathepsin B results in the specific

62 Mice lacking the lysosomal cysteine proteinase cathepsin S (catS) demonstrated a pr

63 mediated HA catabolism, HA modification, and cysteine proteinase (cathepsin B) activation resulting i

64 did not induce expression of proatherogenic cysteine proteinase cathepsins from vascular cells in vi

65 -class inhibitor of the archetypal lysosomal cysteine proteinases cathepsins K, L, and S.

66 SCCA1 neutralizes the papain-like cysteine proteinases, cathepsins (cat) S, L, and K; and

67 We focused on the cysteine proteinases, cathepsins B and L, and their inhi

68 , plasminogen activator and plasminogen, the cysteine proteinases, cathepsins L, S, and K, and the ma

69 In contrast to the other amebic cysteine proteinases characterized so far, which have a

70 1 (SCCA1), inhibit different members of the cysteine proteinase class.

71 Lysosomal cysteine proteinase (CP), proteinase-1(CP7), is the majo

72 The cysteine proteinase-deficient amoeba failed to induce in

73 urification of arginine- and lysine-specific cysteine proteinases, designated gingipains, that consis

74 iciently converted to its active form by the cysteine proteinase dipeptidyl peptidase I.

75 Entamoeba histolytica cysteine proteinases (EhCPs) play a key role in disrupti

76 ania contain an abundance of stage-regulated cysteine proteinases encoded by several gene families.

77 therapeutic agents for pathologies involving cysteine proteinase enzymes.

78 o L cells, including processing by lysosomal cysteine proteinases, even though the former particles l

79 The virulence factors gingipains, cysteine proteinases expressed by P. gingivalis, are lik

80 rt of the region that is homologous with the cysteine proteinase family are removed from the TIN2 cDN

81 referred to as periodontain, belongs to the cysteine proteinase family based on inhibition studies a

82 omology within the carboxy terminus with the cysteine proteinase family of enzymes.

83 lso express cathepsin K, a new member of the cysteine proteinase family whose elastinolytic potential

84 ures of the other members of the papain-type cysteine proteinase family.

85 Extracellular cysteine proteinases from amebic trophozoites are key vi

86 of mir1, mir2, mir3 and other representative cysteine proteinases from protozoa, plants and animals w

87 This leads us to propose a C1 cysteine proteinase function for grass group I allergens

88 Analysis of parasites rendered defective in cysteine proteinase function, either through genetic man

89 ngivalis mutant deficient in lysine-specific cysteine proteinase (gingipain K [Kgp]) resulted in an i

90 The effect of two arginine-specific cysteine proteinases (gingipain Rs) from Porphyromonas g

91 The isolated P-8c2 (cysteine proteinase-glycolipid complex) does not provide

92 Amoebic cysteine proteinases have been proposed as important vir

93 eant peptide aldehyde inhibitors of T. cruzi cysteine proteinases have no effect.

94 bserved with members of the papain family of cysteine proteinases, help to stabilize the complex and

95 uggested that the serpin interacted with the cysteine proteinase in a manner similar to that observed

96 d protein-78 and activation of caspase-12, a cysteine proteinase in the ER, mediating caspase-3 activ

97 dies suggest that SCCA1 inhibits papain-like cysteine proteinases in a manner similar to that observe

98 The critical role of amoebic cysteine proteinases in human gut inflammation and tissu

99 cidins is a novel function of E. histolytica cysteine proteinases in the evasion of the innate immune

100 To test the role of amoebic cysteine proteinases in the pathogenesis of amoebic coli

101 rk, we examined the ability of P. gingivalis cysteine proteinases, including Arg-specific gingipains

102 bility of the RSL of SCCA1 are essential for cysteine proteinase inhibition and that serpins are like

103 he reactive site loop (RSL) is important for cysteine proteinase inhibition.

104 nd both were inhibited by treatment with the cysteine proteinase inhibitor (2S,3S)-transepoxysuccinyl

105 t region surface architecture of the soybean cysteine proteinase inhibitor (soyacystatin N, scN) was

106 Recombinant R1 protein had much greater cysteine proteinase inhibitor activity than recombinant

107 sgenic expression of a biosafe, anti-feedant cysteine proteinase inhibitor and an anti-root invasion,

108 Three cysteine proteinase inhibitor cDNA clones (pL1, pR1, and

109 Cystatin E is thus a functional cysteine proteinase inhibitor despite relatively low ami

110 g cells by either the weak base NH4Cl or the cysteine proteinase inhibitor E-64.

111 heir proteolytic activity was blocked by the cysteine proteinase inhibitor N-alpha-p-tosyl-L-lysine c

112 obtained with the prototypical physiological cysteine proteinase inhibitor, cystatin C.

113 Treatment with the cysteine proteinase inhibitor, E-64, did not prevent thi

114 Analogs of the epoxysuccinyl peptide cysteine proteinase inhibitor, EP-475 (2a), in which the

115 ssion of a cysteine proteinase, instead of a cysteine proteinase inhibitor, may be a novel insect def

116 rees C for 30 min or by treating them with a cysteine proteinase inhibitor.

117 ution could convert SCCA2 into a more potent cysteine proteinase inhibitor.

118 ons for three soybean (Glycine max L. Merr.) cysteine proteinase inhibitors (CysPIs) are inferred fro

119 se genes, in turn, reduced the production of cysteine proteinase inhibitors (CystPIs), which are spec

120 Plant cysteine proteinase inhibitors (phytocystatins) have bee

121 The cystatin superfamily of cysteine proteinase inhibitors consists of three major f

122 Treatment of osteoclasts with cysteine proteinase inhibitors did not affect the number

123 Cysteine proteinase inhibitors have been shown to block

124 The incubation of cultured parasites with cysteine proteinase inhibitors inhibited the denaturatio

125 Specific cysteine proteinase inhibitors interrupted invasion by t

126 ost are one-use suicide substrate serine and cysteine proteinase inhibitors that have evolved to fine

127 were similar; however, the concentrations of cysteine proteinase inhibitors were elevated under A- co

128 Verge protein is dramatically increased by cysteine proteinase inhibitors, suggesting rapid turnove

129 Two genes encoding cystatins, cysteine proteinase inhibitors, were cloned from the fle

130 containing structural motifs in common with cysteine proteinase inhibitors.

131 rk of SCCA1 that independently accounted for cysteine proteinase inhibitory activity.

132 The expression of a cysteine proteinase, instead of a cysteine proteinase in

133 ng hydrolytic enzymes such as cathepsin L, a cysteine proteinase involved in lysis of phagocytosed pa

134 Thus, the malarial cysteine proteinase is a promising target for antimalari

135 ltured parasites, indicating that a malarial cysteine proteinase is required for this process.

136 This cysteine proteinase is responsible for the in vitro clea

137 ases, the mechanism by which they inactivate cysteine proteinases is unknown.

138 aragine endopeptidase (AEP), a pH-controlled cysteine proteinase, is activated during ageing and medi

139 asparagine endopeptidase (AEP), a lysosomal cysteine proteinase, is activated during aging and prote

140 Dipeptidyl-peptidase I, a lysosomal cysteine proteinase, is important in intracellular degra

141 y similar to the caspase family of apoptotic cysteine proteinases, is capable of cleaving and activat

142 se (AVP), the first member of a new class of cysteine proteinases, is essential for the production of

143 d-mouth disease virus encodes two forms of a cysteine proteinase (L protease) which bisects the eIF4G

144 irus, a picornavirus, encodes two forms of a cysteine proteinase (leader or L protease) that bisects

145 phoramidon), serine proteinases (aprotinin), cysteine proteinases (leupeptin) and urokinase (amilorid

146 st that cleavage of IgG by the extracellular cysteine proteinase may limit the effectiveness of the h

147 ns to inhibit both serine and/or papain-like cysteine proteinases may not be a recent event in mammal

148 The virus-encoded cysteine proteinase mediated at least five cleavages (Q(

149 llular acidification required for Hyal-2 and cysteine proteinase-mediated matrix degradation and brea

150 bition of bacterial growth and limitation of cysteine-proteinase-mediated tissue damage.

151 A cDNA clone encoding the 33 kDa cysteine proteinase, mir1, was identified.

152 on of mirolysin with karilysin, as well as a cysteine proteinase of another periodontal pathogen, Pre

153 cleavage of IgG by the extracellular neutral cysteine proteinase of E. histolytica trophozoites, one

154 methods, we demonstrate that lysine-specific cysteine proteinase of P. gingivalis (gingipain K, Kgp)

155 PrtP is a major cysteine proteinase of Porphyromonas gingivalis.

156 er inhibitory activities than L1 against gut cysteine proteinases of the third-instar larvae of weste

157 minal extension, an unusual feature of these cysteine proteinases of trypanosomatids.

158 acid sequences are highly homologous to the cysteine proteinases oryzain gamma and aleurain.

159 Moreover, rLEKTI did not inhibit the cysteine proteinase papain or cathepsin K, L, or S.

160 rent from those that affect catalysis by the cysteine proteinase papain.

161 ate spatial context for interaction with the cysteine proteinase papain.

162 nthesized and evaluated as inhibitors of the cysteine proteinases papain and cathepsin B.

163 iles of these two human cystatins on several cysteine proteinases (papain, clostripain, and calpain I

164 to its role in globin hydrolysis, a malarial cysteine proteinase participates in the dissociation of

165 Studies on members of the papain family of cysteine proteinases, particularly papain (EC 3.4.22.2)

166 es and is most closely related to two legume cysteine proteinases (Phaseolus vulgaris EP-C1 and Vigna

167 Cysteine proteinases play a major role in invasion and i

168 SpeB, a potent extracellular cysteine proteinase, plays an important role in the path

169 y be explained by our discovery that amoebic cysteine proteinases possess IL-1B converting enzyme (IC

170 equencing of the gene encoding porphypain, a cysteine proteinase previously isolated from detergent e

171 The cysteine proteinases referred to as gingipains R (gingip

172 required for the expression of the secreted cysteine proteinase (SCP) of the pathogenic Gram-positiv

173 Tests included cysteine proteinases, serine proteinases, aspartic prote

174 nsible for the removal of the dipeptide is a cysteine proteinase since E-64d, a class-specific inhibi

175 ncluding CSD1, CSD3, and GPX, in addition to cysteine proteinases, some transcription factors, and HR

176 e most thoroughly investigated member of the cysteine proteinase superfamily, have contributed substa

177 ystatin C is a broader-spectrum inhibitor of cysteine proteinases than cystatin SN.

178 em, and one of these, PG0026, is a predicted cysteine proteinase that also contains a CTD, suggesting

179 eomycin hydrolase (BH) is a highly conserved cysteine proteinase that deamidates and inactivates the

180 Bleomycin hydrolase (BH) is a cysteine proteinase that inactivates the anticancer drug

181 pressed in mature ovules, and O141 encodes a cysteine proteinase that is expressed in the outer integ

182 thepsin K is a recently identified lysosomal cysteine proteinase that is the major protease responsib

183 ely via the action of bleomycin hydrolase, a cysteine proteinase that is widely distributed in nature

184 Cathepsins are a group of cysteine proteinases that are involved in various aspect

185 ana encode stage-regulated, cathepsin L-like cysteine proteinases that are leishmanial virulence fact

186 e Picornaviridae enterovirus genus are small cysteine proteinases that catalyze essential cleavages i

187 Calpains are cysteine proteinases that selectively cleave proteins in

188 This first evidence for localization of a cysteine proteinase to the unusual rhoptry secretory org

189 reflects the adaptation of cathepsin L-like cysteine proteinases to diverse functions in parasitic p

190 Inhibitors of calpains and other cysteine proteinases, vesicle trafficking, or lysosomal

191 In previous work, a 33 kDa cysteine proteinase was found in callus initiated from m

192 The 33-kD cysteine proteinase was most abundant in the yellow-gree

193 Using an expanded panel of papain-like cysteine proteinases, we now show that SQN-5, like SCCA1

194 nal clones, mir2 and mir3, encoding putative cysteine proteinases were also identified.

195 This indicates that the cysteine proteinases were most likely responsible for th

196 Cysteine proteinases were the predominant digestive prot

197 Cathepsin B is a lysosomal cysteine proteinase whose expression and trafficking are

198 Thus, acting in concert with cosecreted cysteine proteinases whose activities are constrained to

199 Cathepsin C, another cysteine proteinase with a rather different substrate sp

200 Cysteine proteinases with specificities to cleave either