ライフサイエンスコーパス: cytochrome b5

1 in ActA or the endoplasmic reticulum protein cytochrome b5.

2 presence of an N-terminal domain related to cytochrome b5.

3 been purified to homogeneity and is soluble cytochrome b5.

4 to be involved in binding the heme domain of cytochrome b5.

5 ied that exhibited decreased ability to bind cytochrome b5.

6 s reduced differential absorbance spectra of cytochrome b5.

7 entical to the one elucidated for microsomal cytochrome b5.

8 the lyase reaction, even in the presence of cytochrome b5.

9 m the heme propionate carbonyl carbons in OM cytochrome b5.

10 ) is 48% identical to that of rat microsomal cytochrome b5.

11 s and revealed the transmembrane topology of cytochrome b5.

12 g the several reactions that utilize reduced cytochrome b5.

13 e for interprotein electron self-exchange in cytochrome b5.

14 s, catalyzes the NADH-dependent reduction of cytochrome b5.

15 organ interval flanked by genes Cysteine and Cytochrome B5.

16 tified by mass spectrometry as calumenin and cytochrome B5.

17 wo conserved side chain packing motifs in OM cytochrome b5s.

18 rd chemical and thermal denaturation than Mc cytochromes b5.

19 bilizing isomer A relative to isomer B in Mc cytochromes b5.

20 rkably different properties of rat OM and Mc cytochromes b5.

21 o acid sequence of the full-length house fly cytochrome b5 (134 residues) is 48% identical to that of

22 Cytochrome b5 (a soluble form lacking the 20-amino-acid

23 Cytochrome b5, a microsomal electron-transfer protein, e

24 (CYP101) induced by the soluble fragment of cytochrome b5, a nonphysiological effector of CYP101, we

25 oup of CYP101-bound camphor upon addition of cytochrome b5, a phenomenon also associated with the for

26 he presence of an equimolar concentration of cytochrome b5 and a 10-fold molar excess of NADPH-cytoch

27 % similarity to that of the yeast microsomal cytochrome b5 and a 35% identity and 54% similarity to t

28 ple of an animal flavohemoprotein containing cytochrome b5 and chrome b5 reductase cytodomains.

29 physiological interaction between microsomal cytochrome b5 and cytochrome c (recently reviewed) has i

30 ond-order electron transfer reaction between cytochrome b5 and cytochrome c has been studied by cycli

31 sured for the association between microsomal cytochrome b5 and cytochrome c, clearly indicates that t

32 studies indicate that the binding sites for cytochrome b5 and cytochrome P450 reductase are, as pred

33 teins known to be stable without heme (e.g., cytochrome b5 and hemoglobin apoprotein) are significant

34 oups in the electrostatic binding between OM cytochrome b5 and horse heart cytochrome c was investiga

35 atic stabilization of the complex between OM cytochrome b5 and horse heart cytochrome c.

36 itution with the electron transport proteins cytochrome b5 and NADH-cytochrome b5 reductase.

37 -component redox system comprised of soluble cytochrome b5 and P450 reductase.

38 Cytochrome b5 and phosphorylation enhance 17,20 lyase ac

39 The combination of cytochrome b5 and phosphorylation was not additive.

40 at the structures of bovine liver microsomal cytochrome b5 and rat liver OM cytochrome b5 are almost

41 erized with respect to their ability to bind cytochrome b5 and support substrate oxidation.

42 nate from the formation of a complex between cytochrome b5 and the modified electrode surface which (

43 the removal of the membranous segments from cytochrome b5 and the NADH-cytochrome b5 reductase.

44 1 repeats, a structural motif in common with cytochrome b5, and a HECT domain in common with E6-AP ub

45 orming an electron transfer chain with NADH, cytochrome b5, and NADH-dependent cytochrome b5 reductas

46 with insect NADPH cytochrome P450 reductase, cytochrome b5, and NADPH, the purified CYP4C7 metabolize

47 er microsomal cytochrome b5 and rat liver OM cytochrome b5 are almost identical when compared at medi

48 yme systems, we now show that the actions of cytochrome b5 are independent of the state of P450c17 ph

49 Identification of soluble cytochrome b5 as a member of the reductive activation sy

50 egrees C while the dissociation of heme from cytochrome b5 at room temperature occurs 3 orders of mag

51 Cytochrome b5 augments the 17,20-lyase activity of cytoc

52 mals, a flavohemoprotein (b5+b5R) containing cytochrome b5 (b5) and b5 reductase (b5R) domains.

53 Two acidic residues, Glu-48 and Glu-49, of cytochrome b5 (b5) are essential for stimulating the 17,

54 The membrane heme protein cytochrome b5 (b5) can enhance, inhibit, or have no effe

55 the need for the substrate testosterone and cytochrome b5 (b5) for reduction and as to whether reduc

56 ncreasing ionic strength and the presence of cytochrome b5 (b5) on these two systems.

57 Cytochrome b5 (b5) stimulates the rate of the 17,20-lyas

58 amined are enhanced by the accessory protein cytochrome b5 (b5), but the exact role of b5 in P450 17A

59 trates, and binding of the soluble domain of cytochrome b5 (b5).

60 Cytochrome b5 binding to CYP17A1 is also mutually exclus

61 We have found that Dap1p, like cytochrome b5, binds to heme and that Dap1p targets the

62 (rOM b5) and ferric bovine liver microsomal cytochrome b5 (bMc b5).

63 s to synthesize the Saccharomyces cerevisiae cytochrome b5 by using both LCR and assembly PCR.

64 f full-length microsomal cytochrome-P450 and cytochrome-b5 by solid-state NMR spectroscopy.

65 The small heme-containing protein cytochrome b5 can facilitate, inhibit, or have no effect

66 These data indicate that microsomal cytochrome b5 can play a major role in the in vivo metab

67 , and in vitro plant systems have shown that cytochrome b5 can, at least in some circumstances, also

68 A microsomal cytochrome b5 cDNA from the house fly, Musca domestica,

69 ull-length mammalian 72-kDa cytochrome P450-cytochrome b5 complex in lipid bilayers.

70 ) resonances were monitored as a function of cytochrome b5 concentration by 1H-15N TROSY-HSQC experim

71 The effector activity of the particular rat cytochrome b5 construct used for NMR studies was confirm

72 In vitro studies have demonstrated that cytochrome b5 (Cyb5) also modulates P450 function.

73 ins NADH-cytochrome b5 reductase (CYB5R) and cytochrome b5 (CYB5), it catalyzes the reduction of N-hy

74 finity (a Kd of 13 +/- 3 mM, for the reduced cytochrome b5-CYP-S-CO complex compared to a Kd of 26 +/

75 evious molecular dynamics (MD) simulation of cytochrome b5 (cyt b5) at 25 degrees C displayed localiz

76 (Mb) and its physiological partner protein, cytochrome b5 (cyt b5) can be "symmetrized": the [Mb:cyt

77 We report here on two such ET complexes: cytochrome b5 (cyt b5) in reaction with its physiologica

78 Cytochrome b5 (cyt b5) is a membrane-anchored electron-c

79 Mammalian cytochrome b5 (cyt b5) is a membrane-bound protein capab

80 stigate localized dynamics on the surface of cytochrome b5 (cyt b5) that result in the formation of a

81 th membrane-bound P450 and its redox partner cytochrome b5 (cytb5 ) have been found to be important f

82 uentially delivered from its redox partners: cytochrome b5 (cytb5) and cytochrome P450 reductase, bot

83 Microsomal cytochrome b5 (cytb5) is a membrane-bound protein that m

84 ds that produce various heme proteins (e.g., cytochrome b5, cytochrome b562, and hemoglobin) in the p

85 formed on a solution containing a mixture of cytochrome b5, cytochrome c and polylysine, cytochrome b

86 Cytochrome b5 decreases the apparent Km for P450 reducta

87 tes that FA2H protein contains an N-terminal cytochrome b5 domain and four potential transmembrane do

88 de transmembrane sequences and a part of the cytochrome b5 domain are essential for the basal stabili

89 FA2H lacking the N-terminal cytochrome b5 domain had little activity, indicating tha

90 Expression of the S. cerevisiae FAH1 cytochrome b5 domain in Escherichia coli produces a solu

91 aliana FAH1 gene, which does not contain the cytochrome b5 domain, in the fah1Delta strain produced a

92 84-amino acid protein with an amino-terminal cytochrome b5 domain.

93 tion potential (outer mitochondrial membrane cytochrome b5, Eo = -102 mV; microsomal cytochrome b5, E

94 rane cytochrome b5, Eo = -102 mV; microsomal cytochrome b5, Eo = 3 mV) in the presence of another pro

95 Two distinct forms of cytochrome b5 exist in the rat hepatocyte.

96 Suppressing cytochrome b5 expression in human adrenal NCI-H295A cell

97 ochrome b5 reductase provides electrons, via cytochrome b5, for a range of biochemical reactions in c

98 can instead accept the second electron from cytochrome b5, for human steroidogenic CYP17A1, the cyto

99 ectroscopy reveal properties very similar to cytochromes b5 from vertebrates.

100 In erythrocytes, soluble cytochrome b5 functions in methemoglobin reduction.

101 orientation on electrochemical properties of cytochrome b5 have been performed.

102 yed by the outer mitochondrial membrane (OM) cytochrome b5 heme propionate groups in the electrostati

103 Anti-cytochrome b5 IgG decreased NADPH- and NADH-dependent HE

104 ntally, the interaction of cytochrome c with cytochrome b5 immobilized on a negatively charged monola

105 Interestingly, the addition of cytochrome b5 improved the coupling of the mutant, resul

106 at of NADPH, and assess the possible role of cytochrome b5 in HER formation.

107 human P450-oxidoreductase (OR), and/or human cytochrome b5 in Saccharomyces cerevisiae and analyzed t

108 d out the conditional deletion of microsomal cytochrome b5 in the liver to create the hepatic microso

109 The inclusion of cytochrome b5 in the reconstituted enzyme system is with

110 The inclusion of cytochrome b5 in the reconstituted system of 2D6 and 2C9

111 tion of P450c17 and the allosteric action of cytochrome b5 increase 17,20 lyase activity, but it has

112 sociation constant for manganese-substituted cytochrome b5 increased with temperature from 4 nM at 20

113 The resonance Raman spectrum of MnII cytochrome b5 indicated a high-spin pentacoordinate stru

114 ), the dissociation of manganese substituted cytochrome b5 into the apoprotein and free Mn protoporph

115 indicates that the site of interaction on OM cytochrome b5 is almost identical to the one elucidated

116 When the reduction potential of cytochrome b5 is measured with the aid of several differ

117 The cytochrome b5 is reduced by house fly cytochrome P450 re

118 3 (CER3) and endoplasmic reticulum-localized cytochrome b5 isoforms (CYTB5s).

119 conserved NAD(P)H reductase that contains a cytochrome b5-like domain at the N terminus and a cytoch

120 s two membrane-spanning domains as well as a cytochrome b5-like domain that is characteristic of nonm

121 accharomyces cerevisiae genome data base for cytochrome b5-like sequences identified a 1.152-kilobase

122 able effect on expression of CYP1A1, CYP2D6, cytochrome b5, liver or intestinal fatty acid binding pr

123 as much as 30% of the manganese-substituted cytochrome b5 may dissociate to free Mn-protoporphyrin I

124 rs a mechanism whereby allosteric binding of cytochrome b5 might selectively enhance the lyase reacti

125 In vitro, cytochrome b5 modulates the rate of cytochrome P450-depe

126 and which constitutes part of the surface of cytochrome b5, modulates its reduction potential.

127 decreased by 3.6-fold in hepatic microsomal cytochrome b5 null mice.

128 n the liver to create the hepatic microsomal cytochrome b5 null mouse.

129 50 enzymes showed that in hepatic microsomal cytochrome b5 null NADH-mediated metabolism was essentia

130 similar shifts in the reduction potential of cytochrome b5 occur when it forms a complex with physiol

131 mitochondrial membrane isoform of mammalian cytochrome b5 (OM b5) is considerably more stable than i

132 xial ligands in outer mitochondrial membrane cytochrome b5 (OM cyt b5) has been replaced by a methion

133 d in bacteria, human P450c17 required either cytochrome b5 or phosphorylation for 17,20 lyase activit

134 ion of both flavonoid 3',5'-hydroxylases and cytochrome b5 Overall, this work provides new insight in

135 The NAD(P)H cytochrome b5 oxidoreductase, Ncb5or (previously named b

136 onate located on the exposed heme edge of OM cytochrome b5 participates in the electrostatic binding

137 onate located on the exposed heme edge in OM cytochrome b5 participates in the electrostatic stabiliz

138 The house fly cytochrome b5 protein was overexpressed in Escherichia c

139 ow-spin heme similar to those found in other cytochrome b5 proteins.

140 osomal cytochrome P450 reductase and soluble cytochrome b5 reconstitute the activity of the porcine m

141 The structure and stability of cytochrome b5 reconstituted with manganese protoporphyri

142 emia caused by an erythrocytic deficiency of cytochrome b5 reductase (b5R; type I) in African-America

143 Cytochrome b5 reductase (cb5r) (EC 1.6.6.2) catalyzes th

144 Cytochrome b5 reductase (cb5r), a member of the ferredox

145 gle base pair change in a gene encoding NADH:cytochrome b5 reductase (CBR1, At5g17770).

146 er with the electron transport proteins NADH-cytochrome b5 reductase (CYB5R) and cytochrome b5 (CYB5)

147 asured in CHO cell lines overexpressing NADH:cytochrome b5 reductase (NBR), NADPH:cytochrome P450 red

148 ion of long chain fatty acids 7 (Elovl7) and cytochrome B5 reductase 2 (Cyb5r2) and the synaptic regu

149 NADH cytochrome b5 reductase 3 (CYB5R3) is critical for reduc

150 s reduced to the Fe(2+) state by endothelial cytochrome b5 reductase 3 (CYB5R3, also known as diaphor

151 sights into NO-sGC-cGMP signaling and reveal cytochrome b5 reductase 3 as the first identified physio

152 Mechanistically, we show that cytochrome b5 reductase 3 directly reduces oxidized sGC

153 in vascular smooth muscle cells reveal that cytochrome b5 reductase 3 expression and activity is cri

154 n and form protein-protein complexes between cytochrome b5 reductase 3, also known as methemoglobin r

155 NADH:cytochrome b5 reductase activates the mitomycins to alky

156 anide reductase activity (indicative of NADH:cytochrome b5 reductase activity) coupled to inhibition

157 DNA insertional mutants of the gene encoding cytochrome b5 reductase in Arabidopsis thaliana, CBR1.

158 The results implicate NADH:cytochrome b5 reductase in the differential bioactivatio

159 These results indicate that cytochrome b5 reductase is not essential during vegetati

160 terminus of E3 to the N terminus of the NADH-cytochrome b5 reductase lacking the myristoyl group and

161 In all eukaryotes, NADH:cytochrome b5 reductase provides electrons, via cytochro

162 Because NADH:cytochrome b5 reductase resides predominantly in the mit

163 A cytosolic form of NADH:cytochrome b5 reductase was generated.

164 The orientation of the cytochrome b5 reductase was reversed, from cytosolic to

165 y cell transfectants overexpressing rat NADH:cytochrome b5 reductase were generated.

166 hrome b5-like domain at the N terminus and a cytochrome b5 reductase-like domain at the C terminus.

167 An NADH:cytochrome b5 reductase-transfected clone expressed 9-fo

168 ous segments from cytochrome b5 and the NADH-cytochrome b5 reductase.

169 on transport proteins cytochrome b5 and NADH-cytochrome b5 reductase.

170 with NADH, cytochrome b5, and NADH-dependent cytochrome b5 reductase.

171 ced nicotinamide adenine dinucleotide (NADH)-cytochrome b5 reductase.

172 e binding, while membrane binding of control cytochrome b5 remained unaffected.

173 erric rat liver outer mitochondrial membrane cytochrome b5 (rOM b5) and ferric bovine liver microsoma

174 Cytochrome b5 seems to play a role in HER formation, mos

175 The X-ray crystal structure of rat liver OM cytochrome b5 solved to 2.7 A resolution shows that the

176 The results indicate that cytochrome b5 stimulates a step following the first elec

177 Dap1 is a heme-binding protein related to cytochrome b5 that activates Erg11, so that cells lackin

178 In the presence of cytochrome b5 the variant readily metabolized 17EE and w

179 sed amounts of POR, the allosteric action of cytochrome b5 to promote POR-P450c17 interaction, and Se

180 a possible route for electron transfer from cytochrome b5 to the di-iron centre.

181 cytochrome b5, cytochrome c and polylysine, cytochrome b5 undergoes reversible electrochemistry at t

182 catalyzes the reduction of two molecules of cytochrome b5 using NADH as the physiological electron d

183 The resonance Raman spectrum of MnIII cytochrome b5 was consistent with a high-spin hexacoordi

184 ctric constant on the reduction potential of cytochrome b5 was corroborated by preparing the V45L/V61

185 To achieve these aims, 13C-labeled heme OM cytochrome b5 was expressed in Escherichia coli as previ

186 Interestingly, cytochrome b5 was found to dramatically inhibit both P45

187 Data for cytochrome b5 were analyzed and, when the self-associati

188 Mutants of cytochrome b5 were designed to achieve reorientation of

189 rent K(m) for NADH increased; the K(m)'s for cytochrome b5 were unchanged relative to that of the wil

190 Dap1p is related to cytochrome b5, which activates cytochrome P450 proteins,

191 Cytochrome b5, which augments several P450 activities, i

192 which is activated by a di-iron centre, and cytochrome b5, which regenerates the di-iron centre.

193 thionine synthase in the presence of soluble cytochrome b5 with a Vmax of 2.8 +/- 0.1 micromol min(-1

194 Titrations of [13C]heme-labeled OM cytochrome b5 with horse heart cytochrome c were carried

195 The association of cytochrome b5 with the reduced CYP101-camphor-carbon mon