ライフサイエンスコーパス: cytochrome f

1 equent reduction of both cytochrome b(6) and cytochrome f.

2 ecule lies perpendicular to the long axis of cytochrome f.

3 E123, R157, D188, and the heme on Phormidium cytochrome f.

4 oth with photosystem I (this paper) and with cytochrome f.

5 s oriented with its hydrophobic patch toward cytochrome f.

6 nd was much more accessible to protease than cytochrome f.

7 sis at a stoichiometry of approximately 0.9 (cytochrome f)(-1).

8 absorbance spectra showed a red shift of the cytochrome f alpha-band and the Qy chlorophyll a band in

9 effect of electrostatic interaction between cytochrome f and its electron acceptor in vivo is much s

10 een examined in vitro with mutants of turnip cytochrome f and mutants of pea and spinach plastocyanin

11 ombined deficiencies in both plastid-encoded cytochrome f and nucleus-encoded cytochrome c6 have been

12 Cytochrome f and plastocyanin from the cyanobacterium Ph

13 mining the rate of electron transfer between cytochrome f and plastocyanin has been examined in vitro

14 The reaction between cytochrome f and plastocyanin is a central feature of th

15 ) for intracomplex electron transfer between cytochrome f and plastocyanin were studied, defined by 1

16 Here, the complex of cytochrome f and plastocyanin, electron-transfer protein

17 of the overlap of nonpolar surface areas on cytochrome f and plastocyanin.

18 are required for the efficient insertion of cytochrome f and potentially other subunits of pigment-b

19 to two c-type cytochromes, membrane-anchored cytochrome f and soluble cytochrome c6.

20 affinity site, Cd1, which bridges His143 of cytochrome f and the acidic residue, Glu75, of cyt b(6);

21 omain is not essential for the biogenesis of cytochrome f and the cytochrome b(6)f complex.

22 contact of the positively charged region of cytochrome f and the negatively charged regions of plast

23 3-cpSRP pathway for certain substrates (e.g. cytochrome f and the Rieske protein).

24 oforms of plastid c-type cytochromes, namely cytochromes f and c(6).

25 is unable to synthesize functional forms of cytochromes f and c6 owing to a chloroplast genome mutat

26 s(-1)), for the reduction of plastocyanin by cytochrome f, and approximately 1/30 of k(2)((f-PC)) at

27 targeting of a chloroplast-encoded protein, cytochrome f, and in protein targeting in the etioplast,

28 cells have reduced amounts of cytochrome b6, cytochrome f, and light-harvesting complexes.

29 Spin labels were attached to cytochrome f, and the relaxation enhancements of plastoc

30 -harvesting chlorophyll a/b-binding protein, cytochrome f, and the Rieske FeS protein all showed stro

31 n initial position predominantly proximal to cytochrome f, apparently favored by the reduced ISP, to

32 at the lysine residues of the basic patch on cytochrome f are predominantly involved in long-range el

33 using the coordinates of a truncated turnip cytochrome f as a model.

34 the lysine residues in the positive patch of cytochrome f, as accomplished by mutagenesis, exerts a n

35 n physiological partners cytochrome c(6) and cytochrome f, both from Chlamydomonas reinhardtii.

36 mbrane, had a similar stability to wild-type cytochrome f but was not assembled into the complex.

37 structure of the lumen-side domain of turnip cytochrome f, consisting of Arg209 and Lys187, 58, 65, a

38 The lumen segment of cytochrome f consists of a small and a large domain.

39 ive lumen-side C-terminal fragment of turnip cytochrome f, containing the conserved Lys58,65,66 (larg

40 The integral membrane protein cytochrome f contains an amino-terminal signal sequence

41 vailability of seven different structures of cytochrome f (cyt f) from Chlamydomonas reinhardtii allo

42 aminosum plastocyanin (PC) with P. laminosum cytochrome f (cyt f) was studied using Brownian dynamics

43 tated to alanine and their interactions with cytochrome f (cyt f) were modeled.

44 The interaction of Chlamydomonas cytochrome f (cyt f) with either Chlamydomonas plastocya

45 The electrostatic properties of cytochrome f (cyt f), a member of the cytochrome b6f com

46 ic interaction between plastocyanin (PC) and cytochrome f (cyt f), electron transfer partners in phot

47 Mutations within the signal sequence of cytochrome f (cytf) in Chlamydomonas inhibit thylakoid m

48 other abundant Fe proteins, heme-containing cytochrome f, diiron magnesium protoporphyrin monomethyl

49 owed a considerable decrease in the level of cytochrome f early during dehydration, suggesting that i

50 SP-f)) = 1.5 x 10(6) m(-1) s(-1), for ISP to cytochrome f electron transfer was <10(-2) of the rate c

51 Structure and sequence conservation of the cytochrome f extrinsic portion is concentrated at the he

52 A truncated form of cytochrome f from Chlamydomonas reinhardtii (an importan

53 e for 75% of the kinetic differences between cytochrome f from chloroplasts and the cyanobacterium wh

54 conserved throughout the biological range of cytochrome f from cyanobacteria to plants.

55 electrostatic forces in the interactions of cytochrome f from the cyanobacterium Phormidium laminosu

56 Mutagenesis of cytochrome f from the eukaryotic green alga Chlamydomona

57 Cytochrome f from the photosynthetic cytochrome b(6)f co

58 (b) The efficiency of electron transfer to cytochrome f from the Rieske protein is slightly impaire

59 ture of the functional, extrinsic portion of cytochrome f from the thermophilic cyanobacterium Phormi

60 rounding protein, have a significant role in cytochrome f function.

61 Soluble turnip cytochrome f has been purified from the periplasmic frac

62 sulfur protein (ISP) extrinsic domain to the cytochrome f heme across a distance of 26 A, which is to

63 Cytochrome f(III) quenches triplet state (3)Zncyt c(6),

64 n constant K(a) for zinc cytochrome c(6) and cytochrome f(III) remains (5 +/- 3) x 10(5) M(-1) in the

65 lation of properly targeted plastocyanin and cytochrome f in tha1-ref thylakoid membranes is reduced

66 However, the isotope effect for reduction of cytochrome f in the high potential chain undergoes a pH-

67 ambient pH 7.5-8 was twice that, k(fred), of cytochrome f in the high potential chain, as previously

68 6, with a stoichiometry of 1.0:1 relative to cytochrome f in the highly active M. laminosus complex b

69 idium laminosum in the reaction with soluble cytochrome f in vitro was studied using site-directed mu

70 d by the detection of one of its components (cytochrome f) in chromoplasts using immunoblot and immun

71 The structure of cytochrome f includes an internal chain of five water mo

72 same folding and detailed features as turnip cytochrome f, including (a) an unusual heme Fe ligation

73 est that the post-translational insertion of cytochrome f into the thylakoid membrane uses a SecA-dep

74 Azide inhibited the insertion of cytochrome f into the thylakoid membrane, whereas the io

75 Post-translational integration of cytochrome f into thylakoid membranes was observed after

76 This indicates that the C-terminal region of cytochrome f is important for assembly into the complex.

77 ros), but the half-time for the reduction of cytochrome f is increased (t(12) = 32 ms; for wild type,

78 ariable, so that the surface of P. laminosum cytochrome f is much more acidic than that from turnip.

79 Thus, cytochrome f is not an obligatory intermediate for elect

80 This indicates that covalent haem binding to cytochrome f is not necessary for assembly of the protei

81 of the C-terminal membrane anchor, haem-less cytochrome f is recognized by a thylakoid proteolytic sy

82 length traversed by the ISP in reduction of cytochrome f is twice that of cytochrome b(6).

83 Cytochrome f is unrelated to cytochrome c(1), its functi

84 When the region encoding the cytochrome f leader sequence was replaced with more typi

85 of the cytochrome b(6)f complex both at the cytochrome f level and, independently, at another site c

86 tD mutants, suggesting that the synthesis of cytochrome f may be decreased in the absence of cytochro

87 n to structure-function relationships in the cytochrome f molecule.

88 Two cytochrome f molecules are related by a noncrystallograp

89 ts were obtained with the interaction of the cytochrome f mutant K187E with single, double and triple

90 Interaction of the cytochrome f mutant K187E with the pea plastocyanin muta

91 reduction, both in Chlamydomonas reinhardtii cytochrome f mutants and in the wild type, and (b) the s

92 Structural features of cytochrome f necessary for assembly into the cytochrome

93 Cytochrome f of oxygenic photosynthesis has an unprecede

94 the 70 mV redox potential difference between cytochrome f of P. laminosum and C. reinhardtii (E(m7) =

95 of the interaction between plastocyanin and cytochrome f of P. laminosum in vitro are not optimized

96 the visible spectrum and redox potential of cytochrome f on the identity of aromatic residue 4 provi

97 of the chloroplast-encoded thylakoid protein cytochrome f (pCytf).

98 Rates of cytochrome f photooxidation in all strains were similar

99 x, in agreement with a structural model of a cytochrome f-plastocyanin complex determined by NMR.

100 proposal agrees with solution studies on the cytochrome f/plastocyanin electron-transfer reaction tha

101 A cytochrome f polypeptide unable to bind haem because of

102 se 1,5-bisphosphate carboxylase fused to the cytochrome f precursor.

103 small subunit of Rubisco fused to the turnip cytochrome f precursor.

104 33 amino acid residues resulted in a soluble cytochrome f protein in the thylakoid lumen, indicating

105 in chloroplasts and mitochondria, including cytochromes, FeS proteins, and Fe superoxide dismutase (

106 row in HL and had wild-type levels of Lhcb1, cytochrome f, PsaF, and 2-cysteine peroxiredoxin.

107 ron transfer reactions with plastocyanin] to cytochrome f purified from turnip leaves.

108 igatorily in series, the iron-sulfur and the cytochrome f redox centers in the cytochrome b(6)f compl

109 t does not correlate with strongly inhibited cytochrome f reduction kinetics in the mutants.

110 an the millisecond and microsecond phases of cytochrome f reduction observed in vivo.

111 The rate of cytochrome f reduction was observed to be approximately

112 f plastocyanin and P700 are much faster than cytochrome f reduction, both in Chlamydomonas reinhardti

113 d from the pH dependence of the amplitude of cytochrome f reduction.

114 Subsequent reduction of cytochrome f requires the additional movement of the ISP

115 Expression of the gene for cytochrome f resulted in the production of holocytochrom

116 The 1.96 A structure of turnip cytochrome f revealed a linear internal chain of H2O mol

117 hy, and cross-linking consistently show that cytochrome f self-dimerizes at ionic strengths of 200 mM

118 le the reference E(m) for His-Ntr ligands in cytochrome f should be near that of His-Met hemes.

119 des in a p-side interfacial niche bounded by cytochrome f, subunit IV, and cytochrome b(6), is close

120 of the Rieske iron-sulfur protein (ISP) and cytochrome f subunits of the cytochrome b(6)f complex of

121 complex inhibited the thylakoid insertion of cytochrome f, suggesting competition for a component of

122 ured, demonstrating that a large part of the cytochrome f surface area is sampled by plastocyanin.

123 This increase was larger for P. laminosum cytochrome f than for P. laminosumplastocyanin.

124 identify molecular features of P. laminosum cytochrome f that contribute to the increase, the effect

125 The flash-induced oxidation of cytochrome f, the chloroplast analogue of cytochrome c(1

126 As opposed to PSII and cytochrome f, the light-harvesting antenna complexes of

127 f lysine residues Lys58, Lys65 and Lys187 of cytochrome f to neutral or acidic residues resulted in d

128 same function of transferring electrons from cytochrome f to photosystem I.

129 into the hydrophobic core are inhibitory to cytochrome f translocation, and thus render cells non-ph

130 A mutant cytochrome f unable to bind haem and lacking the C-termi

131 esponding mutants of Chlamydomonas, however, cytochrome f was barely detectable in the DeltapetB or D

132 xtrinsic functional domain of C. reinhardtii cytochrome f was expressed in Escherichia coli without t

133 sfer capability of Chlamydomonas reinhardtii cytochrome f was impaired in site-directed mutants of th

134 Reduction of cytochrome b(6) and cytochrome f was not concerted.

135 ults show that the rate for the oxidation of cytochrome f was unchanged (t(12) = approximately 100 mi

136 oteins ribulose bisphosphate carboxylase and cytochrome f were not affected by the loss of the mangan

137 ctures of the chloroplast and cyanobacterial cytochromes f were compared to explain spectral and redo

138 f the complex, and (iii) it colocalizes with cytochrome f, which migrates between stacked and unstack

139 nserved, buried, and extended water chain of cytochrome f, which provides the exit port for transfer

140 The N-terminal tyrosine of cytochrome f, which provides the sixth ligand to the hem

141 The sole exception is cytochrome f, whose cleavable N-terminal cpSecA-dependen

142 ed evidence for the interaction of Lys187 of cytochrome f with Asp51, Asp42 and Glu43 of plastocyanin

143 out 20% of the wild-type amount of processed cytochrome f with heme attached, apparently assembled in

144 o these differences, the reactions of turnip cytochrome f with P. laminosum plastocyanin and P. lamin

145 h P. laminosum plastocyanin and P. laminosum cytochrome f with pea plastocyanin were examined.