ライフサイエンスコーパス: cytometry

1 ry), and infiltrating cell populations (flow cytometry).

2 igher deformation in real-time deformability cytometry.

3 2',7'-dichlorofluorescein diacetate and flow cytometry.

4 performed by using ELISA, ELISpot, and flow cytometry.

5 IV-uninfected controls were analyzed by flow cytometry.

6 many limitations of fluorescence-based flow cytometry.

7 in intact Ce3D-treated tissues via 3D histo-cytometry.

8 iller (NK) cell number were measured by flow cytometry.

9 re minimal residual disease negative by flow cytometry.

10 a2 integrin adhesion molecules by using flow cytometry.

11 d from spleen and liver for analysis by flow cytometry.

12 hallenging to distinguish with standard flow cytometry.

13 ibodies against pan-cytokeratin through flow cytometry.

14 ransitions across discrete states using flow cytometry.

15 ymph nodes were detected dynamically by flow cytometry.

16 des, and bone marrow were quantified by flow cytometry.

17 ing fluorogenic probes, microscopy, and flow cytometry.

18 completely C3-dependent, as detected by flow cytometry.

19 lthy control subjects was analyzed with flow cytometry.

20 imulated HUVECs and quantified by using flow cytometry.

21 ia Annexin V/Propidium iodide stain and flow cytometry.

22 pecific tetramer(+)CD4(+) T cells using flow cytometry.

23 ain reaction, immunohistochemistry, and flow cytometry.

24 l cycle kinetics were measured by using flow cytometry.

25 ing and non-producing cells purified by flow cytometry.

26 ing antibody-avid polystyrene beads and flow cytometry.

27 mes in epithelial cells was assessed by flow cytometry.

28 Engraftment was assessed by flow cytometry.

29 eviously reported based on conventional flow cytometry.

30 ltures were analyzed by using ELISA and flow cytometry.

31 rom a yeast surface display library via flow cytometry.

32 anoparticle tracking analysis (NTA) and flow cytometry.

33 by surface plasmon resonance as well as flow cytometry.

34 and phagocytic ability were assessed by flow cytometry.

35 he expression of CCR7 was determined by flow cytometry.

36 istochemistry, electron microscopy, and flow cytometry.

37 gene-based phylogenetic microarrays and flow cytometry.

38 ecular composition of human synapses by flow cytometry.

39 ntitative polymerase chain reaction and flow cytometry.

40 g Western blot, electron microscopy and flow cytometry.

41 or injured cells and are detectable by flow cytometry.

42 ing tumors with immunohistochemistry or flow cytometry.

43 f T-cell subsets were measured by using flow cytometry.

44 abeled ECL1i in vivo were detected with flow cytometry.

45 HIV-1-inducible reporter T cell line by flow cytometry.

46 Cell images were acquired with imaging flow cytometry.

47 IS algorithms for image-based cell assays in cytometry.

48 taining, and cell cycle was analyzed by flow cytometry.

49 ism that reduces separation resolution in EP cytometry.

50 on of microglial cells were measured by flow cytometry.

51 heral blood DCs was quantified by using flow cytometry.

52 clerosis patients using multiparametric flow cytometry.

53 d neutrophil apoptosis were analyzed by flow cytometry.

54 ation cohort measured the same parameters by cytometry.

55 or expression were assessed by means of flow cytometry.

56 e infection of hepatocyte cell lines by flow cytometry.

57 as assessed by immunohistochemistry and flow cytometry.

58 by quantitative PCR, immunoblotting and flow cytometry.

59 nestrated) and open aortic repair using flow cytometry.

60 cytes subsets from cultured blood using flow cytometry.

61 yTOF) has greatly expanded the capability of cytometry.

62 osphorylation levels were determined by flow cytometry.

63 mistry, quantitative RT-PCR, ELISA, and flow cytometry.

64 or tonsil, or after ILC2 coculture, by flow cytometry.

65 oliferation and cycle were evaluated by flow cytometry.

66 allergic patients was analyzed by using flow cytometry.

67 grams for the purpose of kinetic single cell cytometry.

68 m in the kidney relies predominantly on flow cytometry.

69 ays and from outgrowth assay readout by flow cytometry.

70 els of common endothelial markers using flow cytometry, 3 subpopulations of endothelial cells could b

71 3D-magnetic twisting cytometry (3D-MTC) is a technique for applying local mec

72 Here we used mass cytometry, a technique that combines single cell analysi

73 Like flow and mass cytometry, Abseq uses specific antibodies to detect epit

74 se before CAR-T cells had no disease by flow cytometry after CAR-T cells.

75 fic effector T cells were analyzed with flow cytometry after polyclonal and pathogen-specific stimula

76 Imaging flow cytometry also showed that CD1a and CD1d proteins were r

77 would support three-dimensional (3D) tissue cytometry: an automated quantitation of immune cells and

78 oma cruzi, as evidenced by transcriptome and cytometry analyses in mixed bone-marrow (BM) chimeras.

79 Finally, flow cytometry analyses indicated significantly lower surface

80 performed whole blood transcriptome and flow cytometry analyses on a total of 70 critically injured p

81 cription polymerase chain reaction, and flow cytometry analyses.

82 Flow cytometry analysis after fluorescent antibody labeling r

83 Flow cytometry analysis indicated the cell cycle was arrested

84 of tissue homogenization necessary for flow cytometry analysis introduces bias and results in the lo

85 focal microscopy of kidney sections and flow cytometry analysis of glomerular cells from magnetic bea

86 dated 24 hours after removal of GNPs by flow cytometry analysis of mitochondrial depolarisation.

87 Using flow cytometry analysis of paraffin-embedded colon tissue, we

88 Flow cytometry analysis of the splenic transitional B cell su

89 zi infection by confocal microscopy and flow cytometry analysis, showing a high expression in macroph

90 confocal laser scanning microscopy and flow cytometry analysis, we demonstrated that protein/lipidoi

91 Using flow cytometry analysis, we found that erf74 and erf74;erf75

92 ts exhibited excellent correlation with flow cytometry analysis.

93 es and mediators was performed by using flow cytometry and a multiplex assay with bronchoalveolar lav

94 Flow cytometry and adoptive transfer of purified cells show t

95 method demonstrated good agreement with flow cytometry and automated hematology analyzers.

96 Multiparameter flow cytometry and automated image analysis of immunostaining

97 eled this small molecule with QD620 for flow cytometry and confocal imaging analyses.

98 Flow cytometry and confocal imaging with QD620-IRS further de

99 ssion on HNECs were determined by using flow cytometry and confocal microscopy.

100 Cell internalization was studied by flow cytometry and confocal microscopy.

101 and cytokine content were quantified by flow cytometry and ELISA, respectively.

102 tic patients and control subjects using flow cytometry and ELISA.

103 oglobulin levels were analyzed by using flow cytometry and ELISA.

104 Here, using real-time deformability cytometry and flicker spectroscopy to define biophysical

105 y donors was prospectively enrolled for flow cytometry and functional experiments.

106 Flow cytometry and gene expression studies demonstrated robus

107 e reprogrammed iCPCs by immunostaining, flow cytometry and gene expression; differentiate iCPCs in vi

108 Repopulation was quantified by flow cytometry and histochemical estimation of dipeptidyl-pep

109 Finally, flow cytometry and immunofluorescence studies showed that alp

110 e cellular and tissue levels (n = 6) by flow cytometry and immunohistochemistry.

111 Although expression assays based on flow cytometry and immunostaining have shown that multidrug r

112 f differentiated cells is performed via flow cytometry and immunostaining to assess quantitative expr

113 olid tumor cells was tested in vitro by flow cytometry and in vivo by PET/CT imaging and dosimetry.

114 Using flow cytometry and intracellular cytokine staining after stim

115 action in vitro and in vivo as shown by flow cytometry and intravital imaging.

116 We performed 6-color flow cytometry and linear mixed-effects modeling to define th

117 this dual-functionality probe for both flow cytometry and mass cytometry in a mimetic cell mixture a

118 al and histological studies, as well as flow cytometry and measurements of proinflammatory mediators.

119 mation-related genes, monitored by both flow cytometry and microarray analysis.

120 Flow cytometry and microscopy studies revealed no detectable

121 Nasal lavage for flow cytometry and nasal swabs for viral PCR were performed a

122 Their immunophenotype was assessed by flow cytometry and protein expression; activation of canonica

123 Flow cytometry and quantitative PCR was used to measure type

124 3-dioxygenase (IDO) were analyzed using flow cytometry and quantitative PCR.

125 ne expression patterns were measured by flow cytometry and quantitative polymerase chain reaction.

126 ned before and after challenge by CyTOF mass cytometry and RNAseq.

127 We apply our method to mass cytometry and scRNA-seq datasets, and demonstrate that i

128 activity of MRP1 were determined using flow cytometry and SECM, and our findings show that these par

129 ication of new high-throughput, high-content cytometry and sequencing technologies.

130 Using single-cell flow cytometry and single-molecule RNA-FISH assays, we demons

131 has been evaluated in combination with flow cytometry and turned out to be around 25% (cells enterin

132 emodynamic flow conditions coupled with flow cytometry and Western blot analysis to elucidate the fun

133 inflammation and sepsis, intracellular (flow cytometry) and secreted cytokines (Luminex), were assess

134 r its detection by ELISA, Western blot, flow cytometry, and confocal microscopy.

135 enotype of immune cells was analyzed by flow cytometry, and cytokines by enzyme-linked immunosorbent

136 l allogeneic responses were measured by flow cytometry, and diapedesis was assessed using transwell p

137 Also, mass spectrometry, flow cytometry, and electron microscopy analyses indicated th

138 ed antigen-responsive cells in PBMCs by flow cytometry, and examined cells in whole blood obtained be

139 Using confocal imaging, PCR, flow cytometry, and functional strategies, we addressed these

140 l blood lymphocyte populations by using flow cytometry, and histologic and ultrastructural analysis o

141 We analyzed cell subsets by flow cytometry, and soluble mediators and antibodies by ELISA

142 yme-linked immunosorbent assay (ELISA), flow cytometry, and Western blot are common bioanalytical tec

143 protein fusions are evaluated in ELISA, flow cytometry, and Western blot experiments and compared to

144 ranscription polymerase chain reaction, flow cytometry, and Western blotting-in several nonprostatic

145 sis and efferocytosis by morphology and flow cytometry; and GCS efficacy by apoptosis assays and qPCR

146 B-cell subsets were quantified by flow cytometry; annexin-V status identified apoptotic cells a

147 signaling, such as the western blot and flow cytometry, are limited in three aspects: 1) The perturbi

148 Analyses by flow cytometry as well as the use of Cldn14-lacZ knock-in rep

149 Applying a capsid flow cytometry assay, we identified two 2'-C-methyladenosine

150 CaValpha2delta1, indicated by two-color flow cytometry assays and confocal imaging, and prevented CaV

151 -145 were used in cell permeability and flow cytometry assays for apoptosis and proliferation.

152 cterized by fluorescence microscopy and flow cytometry assays in BXPC-3 and PANC-1 cells, two pancrea

153 poptosis and cell cycle arrest based on flow cytometry assays.

154 Our flow cytometry-assisted susceptibility test (FAST) method com

155 on (CD) proteins used to count cells by flow cytometry, band 3 (CD233), CD71, CD45, and CD41.

156 Existing mass cytometry barcoding approaches require time intensive la

157 e used for both confocal microscopy and flow cytometry based high-throughput quantification of glutat

158 his predicted structure, we developed a flow-cytometry-based assay that measures cytosolic exchange a

159 Methods A prospective and comprehensive flow cytometry-based immunomonitoring program paralleled the

160 We describe a flow-cytometry-based protocol for intracellular mRNA measurem

161 1 locus in HeLa cells and established a flow cytometry-based screening system to identify compounds t

162 Here we couple mass-cytometry-based single-cell analysis with overexpression

163 mmunohistochemistry (PCNA-staining) and flow cytometry (BrdU incorporation) revealed that a discrete

164 fer high fluorescence signal for use in flow cytometry, but also show better performance in mass cyto

165 In this study, we provide evidence from cytometry by time-of-flight analysis and humanized mice

166 d by means of conventional flow cytometry or cytometry by time-of-flight mass spectrometry, and such

167 e stained with a 38-marker immunophenotyping cytometry by time-of-flight panel.

168 Here we demonstrate that mass cytometry by time-of-flight provides a label-free approa

169 nals were investigated using mass cytometry (cytometry by time-of-flight), which demonstrated that Rv

170 being significantly altered by trauma using cytometry by time-of-flight, RNAseq technology, and func

171 Here, we used a novel technology, mass cytometry by time-of-flight, to comprehensively characte

172 Using cytometry by time-of-flight, we were able to identify se

173 Furthermore, mass cytometry can enumerate AuNPs with a lower detection lim

174 Flow cytometry can provide high-throughput quantification of

175 Combined with histo-cytometry, Ce3D provides a comprehensive strategy for vo

176 re quantitatively and qualitatively via flow cytometry characterized ex vivo and after culture with m

177 crease in Ki compared with WT RGS2 in a flow cytometry competition binding assay).

178 Here, we used flow cytometry, confocal microscopy, and pharmacologic and mo

179 Novel techniques such as mass cytometry could help to identify melanoma biomarkers, al

180 ncrease the concordance between SAB and flow cytometry crossmatch (FCXM) results, thus enabling impro

181 Mass cytometry (CyTOF) has greatly expanded the capability of

182 ellular signals were investigated using mass cytometry (cytometry by time-of-flight), which demonstra

183 The Discriminant Analysis of MultiAspect CYtometry (DAMACY) we present here provides a comprehens

184 of the laboratory procedure and of the flow cytometry data analyses, as well as clinical validation

185 iew some frequently used and accessible mass cytometry data analysis tools, including principal compo

186 examining, visualizing, and presenting mass cytometry data has motivated continuous development of d

187 methods on a large collection of public mass cytometry data sets, measuring intra-cellular signaling

188 nthesize low-dimensional projections of flow cytometry data that typically have a high number of dime

189 n comparing biological conditions using mass cytometry data, a key challenge is to identify cellular

190 ions and highlights novel cell types in mass cytometry data.

191 ne learning to facilitate processing of mass cytometry data.

192 gorithm applied to the high-dimensional mass cytometry dataset identified a cross-validated model con

193 ANJAY, for visualizing high-dimensional flow cytometry datasets.

194 f the open microfluidic electrophoretic (EP) cytometry device.

195 Activation was measured by flow cytometry, ELISA of cultured supernatants, and F-actin s

196 erentiation were determined by means of flow cytometry, ELISA, and multiplex immunoassay.

197 n the airways were assessed by means of flow cytometry, ELISA, Luminex, and immunohistochemistry.

198 Flow cytometry experiments confirmed that both peptides perme

199 In flow cytometry experiments the NPs were twice as bright as tw

200 Using flow cytometry expression of CD38, HLA-DR and PD-1 were measu

201 thelial tissue barrier via multi-colour flow cytometry (FACS).

202 ing phenotypic diversity estimates from flow cytometry (FCM) data of minute amounts of sample.

203 ned mature human skin-derived MCs using flow cytometry for expression of antigen-presenting molecules

204 LG) MK pool (defined by side scatter on flow cytometry) gives rise to a mature high granular (HG) poo

205 atography (HPLC), spectrophotometry and flow cytometry have been used to estimate cyt c.

206 telets to agonists in vitro assessed by flow cytometry (high-responder donors) are cleared more quick

207 : BAT-CD63 upregulation was assessed by flow cytometry; HR-released histamine was quantified by a gla

208 ac-specific heterozygous Bin1 deletion, flow cytometry identified 47% less cBIN1-MPs in plasma, suppo

209 Flow cytometry identified cells expressing CD14 and NRP1.

210 l to mesenchymal transition (EMT) using flow cytometry, immunofluorescence, and quantitative reverse

211 lity is demonstrated by validation with flow cytometry, immunohistochemistry and single-cell RNA-Seq

212 Furthermore, flow cytometry immunophenotyping revealed that rat podocytes

213 tensive in silico analyses and comparison to cytometry immunophenotyping, we show that xCell outperfo

214 OXP3 + CD127dim/-) were evaluated using flow cytometry in 32 patients with cGvHD treated by ECP for a

215 ality probe for both flow cytometry and mass cytometry in a mimetic cell mixture and human peripheral

216 were assessed by immunofluorescence and flow cytometry in T and B cells isolated from human PBMCs obt

217 phils were identified by microscopy and flow cytometry in the lungs and paratracheal lymph nodes.

218 Application and utility of mass cytometry in vaccine development.

219 Here, we used mass cytometry including a broad range of surface markers and

220 Moreover, flow cytometry indicated that cells exited the cell cycle in

221 ICCs were studied by flow cytometry, intracellular electrophysiology, isometric co

222 Overall, (19)F NMR cytometry is a rapid and quantitative method to evaluate

223 ategies currently used in polychromatic flow cytometry is not feasible.

224 High-dimensional flow cytometry is proving to be valuable for the study of sub

225 e differential presence of DPP4 allowed flow cytometry-mediated isolation of senescent cells using an

226 Standardized flow cytometry methods were used to characterize the followin

227 Multicolour Flow Cytometry (MFC) produces multidimensional analytical dat

228 We report the use of Microfluidic Impedance Cytometry (MIC) to characterise the AC electrical (imped

229 gle-cell contraction using magnetic twisting cytometry (MTC).

230 80 cell survival, which was detected by flow cytometry, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny

231 immunohistochemical data, combined with flow cytometry (N = 5) which identified a small number of CA1

232 Flow cytometry of both spleen and lymph node samples revealed

233 Transcriptome analysis and flow cytometry of IL-17A(+)Foxp3(+) cells indicate that Folr4

234 Flow cytometry of intrahepatic T cells isolated from liver bi

235 Membranous location was confirmed by flow cytometry of viable non-permeabilized cells using anti-I

236 We performed flow cytometry on CD3+Va7.2+CD161+ MAIT cells in blood of 55

237 ual disease (MRD) levels as measured by flow cytometry on day 28 of induction I.

238 on for the incorporation of single-cell mass cytometry on the experimental pipeline.

239 nical trials, and quantified by 4-color flow cytometry or allele-specific oligonucleotide real-time q

240 Mass cytometry or CyTOF is an emerging technology for high-di

241 d T cells were isolated and analyzed by flow cytometry or cytokine assays.

242 were analyzed by means of conventional flow cytometry or cytometry by time-of-flight mass spectromet

243 tion of MRD assessment techniques, like flow cytometry or polymerase chain reaction-based methods, ha

244 teins were selected for confirmation by flow cytometry or Western blot.

245 tion-quantitative PCR, microarrays, and flow cytometry, or indirectly, by the presence of oncogenes s

246 Mass cytometry presents an exceptional opportunity to interro

247 In conclusion, the use of single-cell mass cytometry provides a systems-level characterization of C

248 Flow cytometry provides highly sensitive multiparameter analy

249 and kinetics of albumin trafficking by flow cytometry, quantitative confocal microscopy, and an albu

250 Echocardiography, immunostaining, flow cytometry, quantitative real-time polymerase chain react

251 tomic force microscopy, high-resolution flow cytometry, real-time quantitative RT-PCR, and mass spect

252 nd healthy controls, were analyzed with flow cytometry regarding levels of CD23, CD44, CD54, CRTH2, F

253 etric data generated via time-of-flight mass cytometry requires novel analytical techniques because t

254 ing, super-resolution microscopies, and flow cytometry reveal almost 100-fold more efficient co-deliv

255 Single cell mass cytometry revealed that c-Jun activates multiple signali

256 Analysis of cell cycle by flow cytometry revealed that inhibition of tumor cell growth

257 Pneumoniae, using flow cytometry, reverse-transcription polymerase chain reacti

258 monstrate the feasibility of this flow-based cytometry screen to identify both small molecule compoun

259 Flow cytometry showed that trastuzumab facilitated uptake of

260 By flow cytometry, single cells within these cell lines simultan

261 Another strategy is the combination of flow cytometry sorting of antigen-binding B lymphocytes and s

262 Flow cytometry studies revealed that heparin increased the am

263 ry, but also show better performance in mass cytometry than the commercially available counterparts.

264 lement technology to the microscopy and flow cytometry, the microfluidic deformability sensor would p

265 the T cell responses were determined by flow cytometry to be Ag specific.

266 the development of rapid methods using flow cytometry to characterize several aspects of the physiol

267 To address these issues, we utilized mass cytometry to comprehensively profile the effects of chec

268 The trapped cells were analyzed with flow cytometry to detect apoptosis and pyroptosis; 26% were p

269 We used flow cytometry to determine the presence of MBL, defined acco

270 Herein, we use single-cell mass cytometry to dissect the effects of graphene oxide (GO)

271 tomography imaging, gamma counting, and flow cytometry to evaluate the biodistribution, nanomedicines

272 we used high-resolution microscopy and flow cytometry to highlight the heterogeneity of drug activit

273 Utilizing flow cytometry to identify circulating, collagen type 1(+) ce

274 We performed flow cytometry to measure six markers of synaptic subtypes, a

275 We used flow cytometry to quantify expression of the inhibitory recep

276 verexpression studies, mutagenesis, and flow cytometry to show that ICAP1 contains a functional nucle

277 w describe experiments in which we used mass cytometry to simultaneously measure multiple surface mar

278 analyzed for immune cell composition by flow cytometry, Toll-like receptor (TLR) expression by quanti

279 line and a T-DNA insertion mutant using flow cytometry, transactivation and electrophoretic mobility

280 Mass cytometry, used together with these innovative analytic

281 d in a cancer cell line was measured by flow cytometry using a fluorescent imaging probe.

282 ximately 300 proteins by multiparameter flow cytometry using multiple aneuploid model systems such as

283 igh-dimensionality of data generated by flow cytometry usually makes it difficult to visualize.

284 luten tetramer-based assay, we combined flow-cytometry variables in a multiple regression model that

285 Flow cytometry was used for the enumeration and characterizat

286 Polychromatic flow cytometry was used to analyze T-cell activation markers

287 Flow cytometry was used to analyze VEGF-R2 expression in corn

288 Flow cytometry was used to examine T-cell development.

289 Flow cytometry was used to measure IFN-gamma, IL-13, IL-9, IL

290 By means of imaging flow cytometry, we demonstrate a strong and quick binding of

291 Using imaging flow cytometry, we found that both T. gondii and IL-10 inhibi

292 by confocal microscopy and analysis by flow cytometry, we synthesized derivatives of Taxol linked to

293 al studies, in vitro assays and ex vivo flow-cytometry were performed.

294 Immunohistochemistry and flow cytometry were used to identify myeloid cells and neuron

295 SA, western blot, mass spectrometry and flow cytometry were used to screen for autoantibodies, identi

296 to loss of gene expression as judged by flow cytometry, Western blot or immunofluorescence.

297 hat are not easily tracked by intensity-only cytometry, which is affected by heterogeneous protein ex

298 ergic patients were quantified by using flow cytometry with QuantiBRITE beads and compared with total

299 This study combined single-cell mass cytometry with the multiplex analysis of relevant plasma

300 Here we describe a flow cytometry workflow to determine carbapenem susceptibilit