ライフサイエンスコーパス: cytosine methylation

1 g that Pol V alone does not specify sites of cytosine methylation.

2 th epigenetic alterations, including genomic cytosine methylation.

3 ed to be associated with increased levels of cytosine methylation.

4 4 in somatic cells but only when there is no cytosine methylation.

5 t loss of Lsh promotes--as well as prevents--cytosine methylation.

6 iRNA production with pre-existing or de novo cytosine methylation.

7 Ligation-mediated PCR (HELP) assay to study cytosine methylation.

8 sion of AL2 or L2 causes global reduction in cytosine methylation.

9 ed TGS suppression is accompanied by reduced cytosine methylation.

10 n an appreciable drop in the level of global cytosine methylation.

11 t-generation sequencing technology to detect cytosine methylation.

12 that there are redundant pathways leading to cytosine methylation.

13 so by a complex and variable distribution of cytosine methylation.

14 ase excision repair pathway to remove excess cytosine methylation.

15 mediate epigenetic inheritance by specifying cytosine methylation.

16 ut assays, including the HELP assay to study cytosine methylation.

17 e rate-limiting product release steps during cytosine methylation.

18 ate and exhibit a 60-80% decrease in genomic cytosine methylation.

19 ic profiling and intergenomic comparisons of cytosine methylation.

20 ding developmental abnormalities and reduced cytosine methylation.

21 exhibited a 60% reduction of global genomic cytosine methylation.

22 our understanding of the biological role of cytosine methylation.

23 ucleotides is enhanced at least eightfold by cytosine methylation.

24 We measured cytosine methylation (5-mC) content in genomic DNA from

25 e approaches, we propose the hypothesis that cytosine methylation (5mC) and histone methylation H3K36

26 We examined two forms of DNA methylation, cytosine methylation (5mC) and hydroxymethylation (5hmC)

27 In mammals, cytosine methylation (5mC) is widely distributed through

28 chromatin found in higher eukaryotes, namely cytosine methylation (5mC), methylation of histone H3 ly

29 enetic modifications, such as H3K9me3 and C5 cytosine methylation (5mC).

30 Cytosine methylation, a common form of DNA modification

31 Mammalian development requires cytosine methylation, a heritable epigenetic mark of cel

32 ing domain proteins mirrors the detection of cytosine methylation abundance and implicates the presen

33 The use of enzymes sensitive to cytosine methylation allows high-throughput analysis of

34 Bisulfite sequencing allows cytosine methylation, an important epigenetic marker, to

35 mplete pipeline for accurate CpG and non-CpG cytosine methylation analysis at single base-resolution

36 d reproducible results as exemplified by the cytosine methylation analysis of the promoter regions of

37 logic studies that can measure site-specific cytosine methylation and adduct formation will improve o

38 arise from BPDE damage and are influenced by cytosine methylation and BPDE stereochemical considerati

39 spacer (IGS) structure, they showed altered cytosine methylation and chromatin condensation patterns

40 gene expression in CD4+ T cells, we compared cytosine methylation and chromatin structure at the IL-2

41 This is accepted as resulting from cytosine methylation and deamination of 5-methylcytosine

42 inactivation, the functional significance of cytosine methylation and demethylation in mouse embryoge

43 Cytosine methylation and demethylation in tracks of CpG

44 mal epigenetic modification of the genome by cytosine methylation and for cellular differentiation, c

45 ysregulated loci with quantitative assays of cytosine methylation and gene expression.

46 xhibited dramatic reductions of both genomic cytosine methylation and genome-wide H3K9 trimethyl leve

47 have identified Cxxc1 as a regulator of both cytosine methylation and histone 3 lysine 4 trimethylati

48 lectively, the data support a model in which cytosine methylation and histone deacetylation are each

49 nger protein 1 (Cfp1) is a regulator of both cytosine methylation and histone methylation.

50 Epigenetic modifications such as cytosine methylation and histone modification are linked

51 s, especially small RNAs that can direct the cytosine methylation and histone modifications that are

52 h proteins are required for normal levels of cytosine methylation and hydroxymethylation in murine em

53 urthermore, base pair resolution analysis of cytosine methylation and hydroxymethylation with oxidati

54 human brain, including the regulation of DNA cytosine methylation and hydroxymethylation, and a subse

55 transgenic plants led to a reduced level of cytosine methylation and increased expression of a targe

56 DNA cytosine methylation and methyl-cytosine binding domain

57 lator of histone modification in addition to cytosine methylation and reveals one mechanism by which

58 all interfering RNAs (24 nt siRNA) guide the cytosine methylation and silencing of transposons and a

59 times faster than other dinucleotides due to cytosine methylation and the subsequent deamination and

60 required for mammalian embryogenesis, normal cytosine methylation, and cellular differentiation.

61 egion (NOR) decondensation, loss of promoter cytosine methylation, and replacement of histone H3 Lys

62 Stochastic changes in cytosine methylation are a source of heritable epigeneti

63 methylome; and (3) TDCIPP-induced impacts on cytosine methylation are localized to CpG islands within

64 The extent and biological impact of RNA cytosine methylation are poorly understood, in part owin

65 Patterns of DNA cytosine methylation are subject to mitotic inheritance

66 ion assay revealed that M. graminicola lacks cytosine methylation, as expected if its MgDNMT gene is

67 used a novel and highly sensitive genomewide cytosine methylation assay to detect and map genome meth

68 que that can be used to assess the levels of cytosine methylation associated with repetitive DNA sequ

69 ical- and chromosome-specific alterations in cytosine methylation at 2 hpf.

70 IUGR changes cytosine methylation at approximately 1,400 loci (false

71 rfering RNAs (siRNAs) are less abundant, and cytosine methylation at both transgenic and endogenous R

72 In these mutants, symmetric cytosine methylation at CG and CHG motifs is reduced, an

73 SE 1 (MET1) controls faithful maintenance of cytosine methylation at CG sites in repetitive regions a

74 Cytosine methylation at CpG dinucleotides is a critical

75 Cytosine methylation at CpG dinucleotides produces m(5)C

76 iana, small interfering RNAs (siRNAs) direct cytosine methylation at endogenous DNA repeats in a path

77 ted cytosine and a corresponding increase in cytosine methylation at key regulatory regions on the vi

78 ing ATPase DDM1 is specifically required for cytosine methylation at linker histone H1-associated het

79 evidence that Lsh also controls genome-wide cytosine methylation at nonrepeat sequences and relate t

80 nto chromocenters, coincident with losses in cytosine methylation at pericentromeric 5S gene clusters

81 CTCF is sensitive to cytosine methylation at position 2, but insensitive at p

82 Despite widespread loss of cytosine methylation at regulatory sequences, including

83 pigenomic state by revealing its genome-wide cytosine methylation at single base resolution.

84 The oligomers exhibited 5-carbon cytosine methylation at the codon 273 location on the bo

85 Analysis of cytosine methylation at the endogenous MEA-ISR, AtREP2 a

86 rked, stable histone acetylation and loss of cytosine methylation at the IL-2 promoter/enhancer.

87 B1 induction in HepG2 cells is ascribable to cytosine methylation at the promoter, which prevents rec

88 s, and this enhancement of binding is due to cytosine methylation at these sequences.

89 e may perturb the methyltransferase-mediated cytosine methylation at this site, thereby interfering w

90 Here we briefly introduce cytosine methylation before reviewing what is currently

91 ty and cytosine methylation, consistent with cytosine methylation being guided by base-pairing of Pol

92 nomic comparison found marked differences in cytosine methylation between spermatogenic and brain cel

93 ntified in the composition and patterning of cytosine methylation between the two genomes.

94 undergo epigenetic modifications, including cytosine methylation by DNA methyltransferases (DNMTs).

95 0) found that genetic changes were rare, and cytosine methylation changes were frequent.

96 Cytosine-methylation changes are stable and thought to b

97 the loss of H3K4me3 and then subsequent DNA cytosine methylation, changes that were heritable across

98 24-nucleotide (nt) siRNA complementarity and cytosine methylation, consistent with cytosine methylati

99 small unmethylated compartment suggests that cytosine methylation constrains the effective size of th

100 y Islands and Columbia strains also showed a cytosine methylation content at the NORs intermediate be

101 Specifically, we examined the cytosine methylation content of the ribosomal RNA genes

102 Columbia and Cape Verde Islands strains, the cytosine methylation content segregated as an additive M

103 Microarray analysis in a cytosine methylation-deficient mutant of E. coli shows i

104 witch in which concerted changes in promoter cytosine methylation density and specific histone modifi

105 duce transcriptional gene silencing in a DNA cytosine methylation-dependent manner in plants (RNA-dep

106 erous modest ( approximately 20%) changes in cytosine methylation depending on paternal diet, includi

107 These mutations had differing effects on cytosine methylation depending on the element and the se

108 entous fungus Neurospora crassa is marked by cytosine methylation directed by trimethylated Lysine 9

109 Cytosine methylation does not reinforce or replace ances

110 it a decreased plating efficiency, decreased cytosine methylation, elevated global levels of histone

111 IV helps produce siRNAs that target de novo cytosine methylation events required for facultative het

112 r this developmental pattern of low juvenile cytosine methylation followed by higher methylation in a

113 Here, we present the genome-wide map of cytosine methylation for two maize inbred lines, B73 and

114 ic reprograming event involving depletion of cytosine methylation from DNA and histone H3 lysine 9 di

115 mtDNMT1 appears to be responsible for mtDNA cytosine methylation, from which 5hmC is presumed to be

116 tent stem cells the genomic distributions of cytosine methylation, H2A.Z, H3K4me1/2/3, H3K9me3, H3K27

117 A reduction in the DNA modification of cytosine methylation has been linked directly to chromos

118 Cytosine methylation has been shown to regulate essentia

119 Although cytosine methylation has key roles in several processes

120 The distribution of cytosine methylation in 6.2 Mb of the mouse genome was t

121 ound that binding of p53 was not affected by cytosine methylation in a majority of cases.

122 Here, we describe that HELLS controls cytosine methylation in a nuclear compartment that is in

123 port high coverage methylomes that catalogue cytosine methylation in all contexts for the major human

124 subtype- and disease-specific alterations in cytosine methylation in ALL that influence transcription

125 st three pathways control maintenance of DNA cytosine methylation in Arabidopsis thaliana.

126 yze patterns of histone modification and DNA cytosine methylation in cancer and matched normal mucosa

127 ocytes, implicating Dnmt3b in maintenance of cytosine methylation in cancer.

128 Deletion of Lsh alters the allocation of cytosine methylation in chromosomal regions of 50 kb to

129 Cytosine methylation in CpG dinucleotides is believed to

130 me but argue against a direct role for sperm cytosine methylation in dietary reprogramming of offspri

131 identified, and that transcription-targeted cytosine methylation in gene bodies contributes to the p

132 these data, we highlight predicted roles of cytosine methylation in global cellular metabolism provi

133 ptible to RIP, resulting in complete loss of cytosine methylation in M. graminicola.

134 erging from these studies is that removal of cytosine methylation in mammalian cells can occur by DNA

135 5' end of genes and normally protected from cytosine methylation in mammals.

136 Bisulfite sequencing showed significant cytosine methylation in metastatic cell lines that corre

137 dingly, the Bdnf promoter exhibited aberrant cytosine methylation in mutant Htt-expressing cortical n

138 Maintenance of cytosine methylation in plants is controlled by three DN

139 des in two specific contexts consistent with cytosine methylation in S. cerevisiae.

140 of the SUNN promoter revealed low levels of cytosine methylation in the 700-bp region proximal to th

141 presents the first comprehensive picture of cytosine methylation in the epitranscriptome of pluripot

142 Cytosine methylation in the genome of Drosophila melanog

143 into the distribution and dynamic nature of cytosine methylation in the genome.

144 dence linking arsenic exposure with aberrant cytosine methylation in the global genome or at specific

145 Mammalian genomes employ heritable cytosine methylation in the long-term silencing of retro

146 ppressed histone H3 acetylation and enhanced cytosine methylation in the Nlgn1 promoter region and de

147 E(3) was identical to E(2) but showed cytosine methylation in the promoter region and was thus

148 and repeated injections with LY379268 reduce cytosine methylation in the promoters of the three genes

149 We explored the role of cytosine methylation in the regulation of iNOS transcrip

150 ession of Rac2, and reveal the importance of cytosine methylation in the repression of Rac2 expressio

151 Here we show the first evidence for cytosine methylation in the S. mansoni genome.

152 that lack Rac2 expression exhibit increased cytosine methylation in the sequences flanking the gene,

153 The role of cytosine methylation in the structure and function of en

154 ation timing and DNase hypersensitivity with cytosine methylation in two human cell types, unexpected

155 The biological functions of cytosine methylation include host defense, genome stabil

156 cells exhibit a 60 to 80% decrease in global cytosine methylation, including hypo-methylation of repe

157 t overlap sites of 24-nt siRNA biogenesis or cytosine methylation, indicating that Pol V alone does n

158 Treatment with a cytosine methylation inhibitor completely suppressed the

159 Design of novel cytosine methylation inhibitors would be advanced by our

160 DNA cytosine methylation is a central epigenetic marker that

161 DNA cytosine methylation is a central epigenetic modificatio

162 Cytosine methylation is a key epigenetic mark in many or

163 Cytosine methylation is a key mechanism of epigenetic re

164 Thus, DNA cytosine methylation is a regulator of stationary phase

165 Cytosine methylation is an ancient process with conserve

166 Cytosine methylation is an epigenetic and regulatory mar

167 Cytosine methylation is an epigenetic mark that plays an

168 Cytosine methylation is an epigenetic mechanism in eukar

169 DNA cytosine methylation is an epigenetic modification invol

170 Cytosine methylation is an important base modification t

171 DNA cytosine methylation is an important epigenetic regulato

172 Cytosine methylation is critical in mammalian developmen

173 DNA cytosine methylation is crucial for retrotransposon sile

174 by both methyltransferases and the effect on cytosine methylation is dependent on the position of 6-t

175 Understanding cell-to-cell variability in cytosine methylation is essential for understanding cell

176 Cytosine methylation is found in the genomes of many pla

177 Cytosine methylation is important for transposon silenci

178 Cytosine methylation is involved in various biological p

179 Cytosine methylation is mediated by a group of proteins

180 Cytosine methylation is not a general cause of enhanced

181 hanges post-LPS stimulation, suggesting that cytosine methylation is one of the dominant mechanisms d

182 DNA cytosine methylation is one of the major epigenetic gene

183 In mammals, cytosine methylation is predominantly restricted to CpG

184 the remaining majority of the genome, where cytosine methylation is targeted preferentially to the t

185 Cytosine methylation is the major covalent modification

186 Cytosine methylation is the most common covalent modific

187 Intriguingly, cytosine methylation is unaffected at the reactivated lo

188 ggestion is compatible with the finding that cytosine methylation is universal among large-genome euk

189 Cytosine methylation is widespread in most eukaryotic ge

190 Whereas methyltransferases mediate cytosine methylation, it is less clear how unmethylated

191 ough a cyclic enzymatic cascade comprised of cytosine methylation, iterative oxidation of methyl grou

192 3 are methylated in infected plants and that cytosine methylation levels are significantly reduced in

193 ween two major genetic loci affecting global cytosine methylation levels in Arabidopsis.

194 Cytosine methylation levels showed a gradual decrease wi

195 an result in changes to both 24 nt siRNA and cytosine methylation ((m)C) levels in the hybrid.

196 odification in any worm species and link the cytosine methylation machinery to platyhelminth oviposit

197 Here, we report a high-resolution cytosine methylation map of the murine genome modulated

198 eraction with SCN, we generated whole-genome cytosine methylation maps at single-nucleotide resolutio

199 lysine 9, lysine 27 (H3K9me3, H3K27me3), and cytosine methylation (mC), in the normal and cancer geno

200 MS/MS method, the impact of 6-thioguanine on cytosine methylation mediated by two DNA methyltransfera

201 Cytosine methylation might be partially responsible, but

202 Non-CpG cytosine methylation occurs in human somatic tissue, is

203 Cytosine methylation of a relevant promoter of the GR ge

204 describe, for the first time, the extent of cytosine methylation of bacterial DNA at single-base res

205 tion of siRNAs that, in turn, direct de novo cytosine methylation of corresponding gene sequences.

206 s transcription, and studies have shown that cytosine methylation of CpG islands in promoter regions

207 This includes covalent cytosine methylation of DNA and its associated oxidation

208 These changes include cytosine methylation of DNA at cytosine-phosphate dieste

209 Cytosine methylation of DNA CpG dinucleotides in gene pr

210 Cytosine methylation of DNA is an epigenetic modificatio

211 Chromatin modifications, such as cytosine methylation of DNA, play a significant role in

212 R-driven RNA silencing also causes extensive cytosine methylation of homologous DNA in both the trans

213 hereby small interfering RNAs (siRNAs) guide cytosine methylation of homologous DNA sequences.

214 y various mechanisms, including high-density cytosine methylation of L1 promoters and DICER-dependent

215 DNA methyltransferase 3A (DNMT3A) catalyzes cytosine methylation of mammalian genomic DNA.

216 MT) inhibitor 5-aza-2'-deoxycytidine reduces cytosine methylation of the Rac2 gene locus and is suffi

217 We recently identified that cytosine methylation of the SULF2 promoter is associated

218 or suppressor, we tested the hypothesis that cytosine methylation of the TIG1 promoter suppresses its

219 We have correlated cytosine methylation of two epialleles, P1-rr and P1-pr,

220 rs resistance to geminiviruses by increasing cytosine methylation of viral genomes, suggestive of enh

221 d methods, allows one to sensitively measure cytosine methylation on a genome-wide scale within speci

222 Cytosine methylation on CpG dinucleotides is an essentia

223 The disruptive effect of cytosine methylation on nucleosome formation was found t

224 In contrast, effects of endogenous cytosine methylation on the distribution of DNA lesions

225 approach was used to analyze the effects of cytosine methylation on the kinetics of AGT repair of O(

226 uence of DNA sequence context and endogenous cytosine methylation on the kinetics of AGT-dependent re

227 The influence of epigenetic cytosine methylation on the reaction with anti-BPDE at a

228 r physical characteristics and the impact of cytosine methylation on these properties.

229 Inheritance of DNA cytosine methylation pattern during successive cell divi

230 ined molecules with nearly every conceivable cytosine-methylation pattern at each locus.

231 Human DNMT3A is responsible for de novo DNA cytosine methylation patterning during development.

232 Widespread perturbation of cytosine methylation patterning now appears to be a hall

233 Alterations in cytosine methylation patterns are usually observed in hu

234 We found that cytosine methylation patterns at Pl-Blotched were indeed

235 The heritability of cytosine methylation patterns from parent to progeny cel

236 Here, we interrogate cytosine methylation patterns in sperm obtained from mic

237 The consequences of altered cytosine methylation patterns include both inappropriate

238 further observed that specific sequence and cytosine methylation patterns surrounding the targeted g

239 nd noncoding repeats, siRNAs specify de novo cytosine methylation patterns that are recognized by MBD

240 DNA damage could cause heritable changes in cytosine methylation patterns, resulting in human tumor

241 ealed an unappreciated complexity of genomic cytosine-methylation patterns in both tissue derived and

242 e major groove of methylated DNA rather than cytosine methylation per se.

243 lly contradict previous observations of high cytosine methylation polymorphism within Arabidopsis tha

244 Single cytosine methylation polymorphisms are not linked to gen

245 -PCR (nanoHELP) assay to compare genome-wide cytosine methylation profiles between highly purified hu

246 These results show that quantitative cytosine methylation profiling can be used to identify m

247 nalysis technology to complete a large-scale cytosine methylation profiling study involving 47 gene p

248 rations and gene expression, and genome-wide cytosine methylation profiling using the HpaII tiny frag

249 Cytosine methylation promotes deamination.

250 developed, and, together with results from a cytosine methylation protection assay, we determined the

251 Cytosine methylation provides an attractive epigenetic m

252 We use our method to infer cytosine methylation rates at several sites within the p

253 Cytosine methylation regulates essential genome function

254 DNA cytosine methylation regulates gene expression in mammal

255 Cytosine methylation regulates the length and stability

256 ionine (SAMe), the principal methyl donor in cytosine methylation, regulates the methylome dynamics d

257 d proportion of the neuronal genome is under cytosine methylation regulation and provide a new founda

258 ver, their recently described sensitivity to cytosine methylation represents a major bottleneck for g

259 Eukaryotic cytosine methylation represses transcription but also oc

260 Perturbations in cytosine methylation signals are observed in the majorit

261 Cytosine methylation silences transposable elements in p

262 methyltransferase-DNA (MTase-DNA) adducts at cytosine methylation sites.

263 he Arabidopsis SAH1 gene that causes loss of cytosine methylation specifically in non-CG contexts con

264 ndicate that the inheritance of differential cytosine methylation states at NOR loci can be modified

265 uencing (WGBS) make it possible to determine cytosine methylation states at single-base resolution ac

266 r that independently established the correct cytosine methylation status at the target CG of each mol

267 pproaches have been developed to interrogate cytosine methylation status genome-wide, however these a

268 quely, MethylViewer can simultaneously query cytosine methylation status in bisulfite-converted seque

269 Accurate determination of cytosine methylation status in promoter CpG dinucleotide

270 activity can be manipulated by altering its cytosine methylation status in vitro.

271 Cytosine methylation status was also followed in in vitr

272 lated mutations and epimutations (changes in cytosine methylation status) in mutation accumulation (M

273 thylation analysis and rapid quantitation of Cytosine methylation suitable for a wide range of biolog

274 th the previous finding of reduced levels of cytosine methylation, these data indicate that cells lac

275 that is critical for normal distribution of cytosine methylation throughout the murine genome.

276 small interfering RNAs (siRNAs), which guide cytosine methylation to corresponding sequences, require

277 Epigenetic modification of DNA by cytosine methylation to produce 5-methylcytosine (5mC) h

278 Large-genome eukaryotes use heritable cytosine methylation to silence promoters, especially th

279 ittle is known of the mechanisms that direct cytosine methylation to specific sequences.

280 Genome losses in cytosine methylation upon SmDnmt2 silencing and the iden

281 lation with trichostatin A (TSA) or blocking cytosine methylation using 5-aza-2'-deoxycytosine (aza-d

282 Summary We conducted genome-wide mapping of cytosine methylation using methylcytosine immunoprecipit

283 matin marks (H3K4me3, H3K36me2, H3K27me3 and cytosine methylation) using publicly available data.

284 hite, allowing genome-wide quantification of cytosine methylation via high-throughput sequencing.

285 ethyltransferase enzymes (DCMTases) catalyze cytosine methylation via reaction intermediates in which

286 While cytosine methylation was assayed in various regions span

287 Variation in cytosine methylation was particularly dramatic over tand

288 ns were similar among all lines studied, but cytosine methylation was slightly more prevalent in the

289 The catalytic mechanism of DNA cytosine methylation was studied by structurally and fun

290 hailandensis, revealed that, upon induction, cytosine methylation was targeted specifically to the ph

291 itioning of nucleosomes can be attenuated by cytosine methylation when a short run of CpG dinucleotid

292 pus, exhibited rapid and dramatic changes in cytosine methylation when DNMT activity was inhibited.

293 t commonly studied epigenetic alterations is cytosine methylation, which is a well recognized mechani

294 rimary effect of aza-C is the prohibition of cytosine methylation, which results in covalent methyltr

295 We conclude that the relationship of cytosine methylation with heterochromatin is not simple

296 ined epigenetic heterogeneity as assessed by cytosine methylation within defined genomic loci with fo

297 In contrast, cytosine methylation within p53 codon 158 slightly incre

298 Cytosine methylation within RNA is common, but its full

299 as cells that express Rac2 exhibit increased cytosine methylation within the body of the Rac2 gene.

300 Cytosine methylation within the gene encoding for FK506