ライフサイエンスコーパス: cytosolic fraction

1 pairing such abnormal proteins mainly in the cytosolic fraction.

2 U/g wet wt or 0.07 +/- 0.03 U/mU CrK in the cytosolic fraction.

3 fraction (72%), but 28% was found within the cytosolic fraction.

4 n of nonisoprenylated Rho and Rac GTPases in cytosolic fraction.

5 duced total p130(Cas) translocation from the cytosolic fraction.

6 Stat3 exhibited a strong requirement for the cytosolic fraction.

7 tion differed depending on the source of the cytosolic fraction.

8 nd Bax were predominantly present in a crude cytosolic fraction.

9 to the NM concomitant with a decrease in the cytosolic fraction.

10 Pex19p localizes predominantly to the cytosolic fraction.

11 uclear fraction and approximately 10% in the cytosolic fraction.

12 pressed low levels that were confined to the cytosolic fraction.

13 he preponderance of p27Kip1 was found in the cytosolic fraction.

14 not result in a concomitant decrease in the cytosolic fraction.

15 type and DeltaF508 CFTR can be found in the cytosolic fraction.

16 lithium very little tau was detected in the cytosolic fraction.

17 ble of cleaving Bax which is absent from the cytosolic fraction.

18 imately 50-kDa FLAG-NMT predominantly in the cytosolic fraction.

19 ilized cells was as efficient as that of the cytosolic fraction.

20 ction (15% of protein; 8% of activity) and a cytosolic fraction.

21 ted by a reaction that depended on ATP and a cytosolic fraction.

22 However, both increased 5LO levels in the cytosolic fraction.

23 on the amount of Pt bound to proteins in the cytosolic fraction.

24 er in the mitochondrial fraction than in the cytosolic fraction.

25 GF stimulated synthesis of 15(S)-HETE in the cytosolic fraction.

26 stern blot analysis was localized within the cytosolic fraction.

27 as redistributed from the particulate to the cytosolic fraction.

28 rendered mtTrx to be solely expressed in the cytosolic fraction.

29 vered essentially equally in particulate and cytosolic fractions.

30 the 68-kDa polypeptide was localized to the cytosolic fractions.

31 ed in membrane-enriched fractions but not in cytosolic fractions.

32 nificantly elevated in both the membrane and cytosolic fractions.

33 increased total PKC activity in membrane and cytosolic fractions.

34 ticulate fraction, but not in the nuclear or cytosolic fractions.

35 e nuclear fraction, not with the membrane or cytosolic fractions.

36 rylated GRK5 is found in plasma membrane and cytosolic fractions.

37 and MsrA was detected in both cell wall and cytosolic fractions.

38 ions and concurrent decreases in PKCalpha in cytosolic fractions.

39 B-crystallin, and MKBP were predominantly in cytosolic fractions.

40 beta-mercaptoethanol cell wall extracts and cytosolic fractions.

41 P3 membrane-enriched fractions and little in cytosolic fractions.

42 as separated at 45,000g into particulate and cytosolic fractions.

43 ent period and partitioned into nuclear, not cytosolic, fractions.

44 o date (54%) compared with proteins from the cytosolic fraction (35%).

45 idence of lineage-specific proteins than the cytosolic fraction (57% and 73% homologue incidence resp

46 a Km for cAMP of 3.3 +/- 0.6 microM, and the cytosolic fraction a Km of 5.4 +/- 2.8 microM.

47 eased in particulate fractions compared with cytosolic fractions after habituation training and was m

48 , the preferential processing of ICAD in the cytosolic fraction allows the translocation of DFF40/CAD

49 h a 50-fold increase in PLA2 activity in the cytosolic fraction, an increase in agonist-stimulated ar

50 ealed a band (15 kDa) of cytochrome c in the cytosolic fraction and a corresponding decrease in the m

51 tive Gbeta5 protein complex from the retinal cytosolic fraction and identified the protein tightly as

52 rome c release from the mitochondrial to the cytosolic fraction and increased cleavage of caspases 3

53 OSBP1 is detected mainly in the soluble or cytosolic fraction and nuclei whereas OSBP2 is detected

54 nd atorvastatin increased COX2 levels in the cytosolic fraction and the membranous fraction.

55 uitinated proteins in both nucleoprotein and cytosolic fractions and decreased unconjugated ubiquitin

56 observation that pgp3 was highly enriched in cytosolic fractions and had a minimal presence in the in

57 Immunoblot analysis of nuclear and cytosolic fractions and immunohistochemistry revealed th

58 CRHSP-28 was present in a 60-kDa complex in cytosolic fractions and in a high molecular mass complex

59 etermine 4F binding IRF5 in endothelial cell cytosolic fractions and to confirm biolayer interferomet

60 ee system (Sph kinase obtained from platelet cytosolic fractions) and much stronger than that by DL-t

61 00 x g soluble and membrane fractions of the cytosolic fraction, and 3) with no significant effects o

62 and involucrin proteins were detected in the cytosolic fraction, and involucrin levels increased afte

63 tions, the betaII isoform was reduced in the cytosolic fraction, and the betaI and gamma isoforms wer

64 al partitioning resulted in a loosely bound (cytosolic) fraction, and a tightly bound (nuclear) fract

65 nate in both a light membrane fraction and a cytosolic fraction; and (iii) MTMR13 membrane associatio

66 an RFC antiserum recognized two bands in the cytosolic fraction (approximately 60 kDa and approximate

67 p532 has been located in the cytosolic fraction as well as in the Golgi apparatus.

68 d the K(+)-induced loss of [14C]ACh from the cytosolic fraction as well as the K(+)-induced gain of [

69 ctions showed selective association with the cytosolic fraction, as documented by copurification with

70 We determined that PR in the cytosolic fraction associated with hsp90 and p23.

71 Further analysis of this cytosolic fraction at 100,000 x g indicates that the COX

72 iol increased P-STAT3 in the ischemic cortex cytosolic fraction at 3 h after MCAO without affecting T

73 nfection and was highly enriched in the host cytosolic fraction but absent in the purified chlamydia

74 Most of the protein was localized in the cytosolic fraction, but a small fraction was detected in

75 tate, RhoA was observed predominantly in the cytosolic fraction, but it was translocated in part to t

76 tate, RhoA was observed predominantly in the cytosolic fraction, but it was translocated in part to t

77 pp37 was purified from the cytosolic fraction by anti-Tyr(P) affinity chromatograph

78 d purified 71.2-fold to homogeneity from the cytosolic fraction by chromatography with concanavalin A

79 ated pool of p190RhoGAP between membrane and cytosolic fractions by a mechanism that requires direct

80 crosomal fractions by inhibiting PDE3 and in cytosolic fractions by inhibiting both PDE3 and PDE1.

81 Western blot analysis of nuclear and cytosolic fractions confirmed that there was no transloc

82 not observed when Ca(2+) was applied to the cytosolic fraction containing sarcoplasmic reticulum.

83 The activity present in the cytosolic fraction could be depleted with antibodies to

84 ectrophoretic mobility shift assay using the cytosolic fractions demonstrated that this flavone elici

85 e ligand-binding assays, using [(3)H]TCDD in cytosolic fractions, demonstrated that 3'-methoxy-4'-nit

86 port along microtubules and diffusion in the cytosolic fraction exhibit anomalous (nonFickian) behavi

87 applied a rapid filtration method to obtain cytosolic fractions for Western blotting.

88 The cytosolic fraction from lung and bronchial alveolar lava

89 Finally, gel filtration analysis of cytosolic fractions from 293 cells that were coexpressin

90 n phosphorylation, tubulin was isolated from cytosolic fractions from anti-IgM-activated B-cells (DT4

91 , synaptic vesicle-enriched and synaptosomal cytosolic fractions from rat brain, as well as the mitoc

92 glucose, was decreased 88% in dialyzed liver cytosolic fractions from selenium-deficient rats.

93 Screening of cytosolic fractions from several cell types and rat brai

94 Cytosolic fractions from SNAP-treated thymocytes cleaved

95 The particulate and cytosolic fractions from the double disruption (lpp1Delt

96 s; however, cytochrome c was not detected in cytosolic fractions from these cells.

97 veloped and used to screen a large number of cytosolic fractions from various tissues.

98 the biochemical analysis of the nuclear and cytosolic fractions further confirmed the nuclear transl

99 The enzyme, which was recovered in the cytosolic fraction, has a neutral pH optimum, and dithio

100 , whereas no aggregation was observed in the cytosolic fraction in a 3-day period.

101 shifted from the membranous fraction to the cytosolic fraction in the cdc43 mutants, and levels of t

102 of NF-kappaB levels in both the nuclear and cytosolic fractions in a time-dependent manner.

103 -type formin is detected in both nuclear and cytosolic fractions; in contrast, the truncated formins

104 y and sedimentation velocity analyses of the cytosolic fraction indicate that HPS1 and HPS4 form a mo

105 levels of cytochrome c were increased in the cytosolic fraction, indicating a decline in mitochondria

106 Partitioning of kinesin between vesicle and cytosolic fractions is highly sensitive to buffer compos

107 -9 and caspase-3 was markedly decreased in a cytosolic fraction isolated from HeLa cells with reduced

108 Titration of the 5'-UTR with a cytosolic fraction isolated from HuH-7 grown in the pres

109 magnesium was maximally demonstrable in the cytosolic fraction; it was markedly reduced by okadaic a

110 The presence of the enzyme in the cytosolic fraction, its dependence on a reducing environ

111 lied by the isolation of the cFTase from the cytosolic fraction, its high affinity for its substrates

112 lpha-synuclein is found predominantly in the cytosolic fractions, membrane-bound alpha-synuclein has

113 icase in a cell extract was inhibited by the cytosolic fraction obtained from gef1Delta yeast.

114 d -epsilon isoforms between the membrane and cytosolic fractions occurred in response to 100 pM AVP.

115 rajectories of microspheres migrating in the cytosolic fraction of a cellular extract.

116 Applying this approach to the crude cytosolic fraction of Arabidopsis thaliana leaves using

117 The cytosolic fraction of both cell types stimulated the act

118 of protein substrates for this enzyme in the cytosolic fraction of brain, heart, liver, and erythrocy

119 ntage of Ras-GRF from the particulate to the cytosolic fraction of cells and renders the protein inse

120 spho-FoxO3a (inactive form) increased in the cytosolic fraction of cells treated with iron in a PI3K-

121 have detected caspases -8 and -3 only in the cytosolic fraction of cells, but caspases -2, -7, and -9

122 The latter two activities are present in a cytosolic fraction of Crithidia fasciculata, which is ea

123 three different column chromatographies of a cytosolic fraction of frog photoreceptor outer segments,

124 the endonuclease is poorly expressed in the cytosolic fraction of healthy SK-N-AS cells.

125 The cytosolic fraction of hT17M Rho retinal cells was used t

126 e-stabilized nNOS.hsp90 heterocomplex in the cytosolic fraction of human embryonic kidney 293 cells s

127 is catalyzed by an enzyme(s) located in the cytosolic fraction of kidneys.

128 tate transaminases, and DNA fragments in the cytosolic fraction of liver homogenates were higher in c

129 e mitochondrial swelling and increase in the cytosolic fraction of mitochondrial intermembrane protei

130 coli and COS-1 cells, respectively, and the cytosolic fraction of mononuclear leukocytes from NAT1*4

131 B56 epsilon PP2A subunits is observed in the cytosolic fraction of mouse retina, the phosducin dephos

132 raction with a corresponding decrease in the cytosolic fraction of myocardial lysates from hearts sub

133 Levels of TGase activity in the cytosolic fraction of old chondrocytes were 7-fold highe

134 of CaMKII-beta but not alpha protein in the cytosolic fraction of Pb2+-exposed rats.

135 as purified approximately 1275-fold from the cytosolic fraction of pig kidney, and the properties of

136 , GFPP) was purified about 560-fold from the cytosolic fraction of pig kidney.

137 It is concluded that the cytosolic fraction of porcine aorta contains a protein f

138 Recent evidence suggests a cytosolic fraction of PrP (cyPrP) functions either as an

139 % proteome coverage, are identified from the cytosolic fraction of S. oneidensis, while requiring <50

140 ntrast, a 38-kDa species was detected in the cytosolic fraction of SY5Y cells.

141 pha was translocated from the particulate to cytosolic fraction of TF-1 cells within 5 minutes of tre

142 fragment of PIDD (PIDD-CC) increased in the cytosolic fraction of the hippocampal CA1 subregion and

143 tly elevated in the membrane, but not in the cytosolic fraction of the hippocampus of AD subjects.

144 nto 5-oxo-ETE was found predominantly in the cytosolic fraction of the macrophage, with an approximat

145 restricted eNOS mutant (myr-) as well as the cytosolic fraction of the palmitoylation-deficient eNOS

146 The cytosolic fraction of the tumor suppressor p53 activates

147 t membrane-associated and thus appear in the cytosolic fraction of thymocyte and splenocyte extracts.

148 also induced the appearance in membrane and cytosolic fractions of a PKCalpha-immunoreactive band th

149 nd syntaxin 2 were found in the membrane and cytosolic fractions of cells, whereas syntaxin 4 was det

150 in mitochondria, and to a lesser extent, in cytosolic fractions of cultured astrocytes.

151 f the BH3 domains requires both membrane and cytosolic fractions of cytoplasm, involves the release o

152 ate levels of Hsp70 in the mitochondrial and cytosolic fractions of different tissues of diabetic rat

153 ncreased in membrane fractions and decreased cytosolic fractions of GK soleus muscles.

154 TIP47 was found primarily in the cytosolic fractions of HeLa cells and murine MA10 Leydig

155 Immunoblots from membrane and cytosolic fractions of isolated PF cells revealed conven

156 GFbeta-IIR was detected both in membrane and cytosolic fractions of MDA-231 tumor cells as well as in

157 sed PKCalpha and PKC in the membrane and the cytosolic fractions of mice hippocampus.

158 levels of phospho-PKC-zeta were lower in the cytosolic fractions of OPN knock-out mice hearts as comp

159 uccinyl-CoA synthetase activity was found in cytosolic fractions of pancreatic islets.

160 chelerythrine stimulated PKC activity in the cytosolic fractions of rat and mouse brain in concentrat

161 edly, FOX activity was noted in membrane and cytosolic fractions of rat enterocytes.

162 CC1 protein levels in the plasma membrane or cytosolic fractions of spinal cords.

163 d to be degraded in both the particulate and cytosolic fractions of TF-1 cells after treatment with a

164 determine the capacity of proteins found in cytosolic fractions of the bovine adrenal medulla to bin

165 ty was associated with both the membrane and cytosolic fractions of the cell, and the membrane-bound

166 s in Mrp2-null mice were observed in DNA and cytosolic fractions of the kidneys.

167 in chelatable iron in mitochondrial but not cytosolic fractions of the rat hippocampus.

168 purified by RNA affinity chromatography from cytosolic fractions of TNF-alpha-stimulated human umbili

169 proteins derived from both the membrane and cytosolic fractions of tumor cell lysates.

170 1 beta were induced by the membrane, but not cytosolic, fraction of M. tuberculosis.

171 hate (GTP)-binding proteins from membrane to cytosolic fractions on Western blots, suggesting that lo

172 In cytosolic fractions, PDE3A-118 and PDE3A-94 comprise >50

173 Endoproteinase LysC digestion of the cytosolic fraction produced a global proteomic sample, c

174 ocalized to detergent-sensitive membrane and cytosolic fractions, respectively.

175 tern blot analysis of the microsomal and the cytosolic fractions revealed that the induced expression

176 s containing nuclei or purified DNA, but not cytosolic fractions, stimulated signaling through TREM2.

177 DR1 protein is not entirely localized in the cytosolic fraction, suggesting that it may interact with

178 the mutants are primarily distributed in the cytosolic fraction, suggesting that these mutations disr

179 pitates from nuclear fractions (but not from cytosolic fractions) the upstream binding factor, which

180 Within the cytosolic fraction, the enzymes of glycolysis, fatty aci

181 inase II were 81 and 19%, whereas within the cytosolic fraction, the relative contributions for hexok

182 ppearance of high-molecular-weight TDP-43 in cytosolic fractions, these findings demonstrate the misl

183 TRADD, FADD, the caspase-8, and TRAF2 in the cytosolic fraction, TNFR1 in the mitochondria was associ

184 ive PAP-1 activity was translocated from the cytosolic fraction to the membrane fraction within 2 min

185 redistribution of TIP47 and adipophilin from cytosolic fractions to the lipid droplet fraction.

186 ignificant portion of TIP47 shifted from the cytosolic fractions to the lipid droplet fractions.

187 NEDD4-1 was identified from Jurkat cytosolic fractions using an enzyme-dead Mdm2 mutant pro

188 rosocysteine, the rate of degradation in the cytosolic fraction was 4.1 +/- 0.3 nmol.min-1.(mg protei

189 However, as expected, p27Kip1 in the cytosolic fraction was greatly reduced following serum s

190 ccordingly, a decrease of PKC content in the cytosolic fraction was observed.

191 ual transcripts in the 'membrane-bound' and 'cytosolic' fractions was quantitated for thousands of ge

192 d Akt and translocation of Galphaq/11 to the cytosolic fraction were maintained in Tg-Y319F hearts.

193 Crude liver homogenates and their cytosolic fractions were assayed for their chymotrypsin-

194 increased oligonucleosomes in the epidermal cytosolic fractions were detected in the diseased mice.

195 informed consent), and cellular nuclear and cytosolic fractions were generated by subcellular fracti

196 1-/- cells, as levels of FGF2 in nuclear and cytosolic fractions were not significantly different.

197 testinal FOXs in which separate membrane and cytosolic fractions were prepared and FOX activity was m

198 oblot analysis, after lysis, the nuclear and cytosolic fractions were separated and analyzed by SDS-P

199 tion with membrane fractions, rather than in cytosolic fractions, where most heterologously expressed

200 d an interaction between 5LO and COX2 in the cytosolic fraction, whereas when H-89 was added, 5LO int

201 Furthermore, pretreatment of the cytosolic fraction with anti-PP2A serum attenuated the g

202 cantly increased in both the nuclear and the cytosolic fractions with denervation.