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1 dAMP and dGMP were found to be inserted opposite these O
2 dAMP is a weak competitive inhibitor with a Ki of approx
5 observed the exclusive formation of [32P]-3'-dAMP and no polymeric ADP-ribose molecules following che
6 the presence of Mg(2+), AMP, 2'-dAMP, and 3'-dAMP are incorporated into sym/sub by 3D(pol) at rates o
7 The position of the phosphate in 5'- and 3'-dAMP is observed to deactivate radical formation at the
9 5'-dAMP > 5'dTMP >> 5'-dGMP and 3'-dGMP > 3'-dAMP approximately equal to 3'-dCMP approximately equal
11 lumination of A(-H)* in dAdo, 3'-dAMP and 5'-dAMP in aqueous glasses at 143 K leads to 80-100% conver
12 of exchange following the trend 5'dCMP > 5'-dAMP > 5'dTMP >> 5'-dGMP and 3'-dGMP > 3'-dAMP approxima
15 o-furan-2-yloxymethyl]-phosphonic acid) is a dAMP (2'-deoxyadenosine monophosphate) analog that maint
17 ition showed a 10-fold preference for adding dAMP to the ends of DNA over that of the other three nuc
20 -dependent phosphorylation of AMP to ADP and dAMP to dADP, can also catalyze the conversion of nucleo
22 2-dG adducts pair preferentially to dCMP and dAMP during translesional synthesis in a process that is
23 567A mutant of RB69pol inserts both dCMP and dAMP opposite 8-oxoG rapidly and with equal efficiency.
24 ol alpha catalyzed incorporation of dCMP and dAMP opposite all four stereoisomers of dG-N2-tamoxifen,
25 both pol kappa and pol eta inserted dCMP and dAMP opposite the 4-OHEN-dC and extended past the lesion
26 g spectra; direct incorporations of dCMP and dAMP were observed, along with lesser amounts of dGMP an
27 Pol beta promoted incorporation of dCMP and dAMP, along with small amounts of one-base and two-base
29 deficient cells can be prevented by dGMP and dAMP supplementation, providing conclusive evidence that
31 incorporated incorrect nucleotides, dGMP and dAMP, opposite the lesion more preferentially than the c
32 und substantial misincorporation of dTMP and dAMP opposite 2-AP-6-SCH3 and 2-AP-6-SO3H, respectively.
33 ol alpha catalyzed incorporation of dTMP and dAMP opposite epsilon dC, accompanied by lesser amounts
38 eoxyadenosine 5'-monophosphoryl group (2'-Az-dAMP) from the analogous 5'-triphosphate (2'-Az-dATP) on
41 ctural identities of the novel BPQ-dGMP, BPQ-dAMP, and BPQ-dCMP adducts were confirmed by acid phosph
45 accompanied by much smaller amounts of dCMP, dAMP, and dGMP and some one- and two-base deletions.
47 cleavage sites for the enzyme by deaminating dAMP and dCMP in DNA to dIMP and dUMP, respectively.
48 bility of bypass polymerases to insert dTMP, dAMP, or dGMP opposite 1,N(6)-gamma-HMHP-dA and detected
49 remely high fidelity, misincorporating dTMP, dAMP, and dGMP opposite a template G target with efficie
51 -DNA adduct, promoted small amounts of dTMP, dAMP, and dGMP misincorporation opposite the lesion (tot
55 h sequence contexts, whereas the Vmax/Km for dAMP incorporation increased 4.7-fold when the base pair
57 nts of HeLa extract, CldAMP substitution for dAMP within the TATA box decreased in vitro pol II trans
58 ra-AMP-terminated DNA (Km = 7.1 pM) than for dAMP-terminated DNA of otherwise identical sequence (Km
61 ns opposite dG-AAF followed the order dCMP > dAMP > dGMP > dTMP; the frequency of dNTP insertion oppo
63 both polymerases preferentially incorporate dAMP opposite the natural abasic site and tetrahydrofura
65 blunt-end additions only if all incorporated dAMPs are extrahelical, leading to predominantly single
67 l kappa bypassed the lesion by incorporating dAMP and dCMP, respectively, opposite the lesion and ext
69 ed the correct dTMP as well as the incorrect dAMP opposite the DE-dA adducts derived from both BaP an
70 gly, oxidation of 8-oxoG was found to induce dAMP and dGMP insertion opposite the lesion by Kf exo- w
72 the 5'-T of the dimer, whereas it can insert dAMP with efficiency comparable to that opposite the 3'-
74 nly Y-family member to preferentially insert dAMP opposite 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxo
76 vicinity of the abasic lesion and inserting dAMP with a frequency of 67% opposite the abasic site.
77 se from bacteriophage RB69 (RB69pol) inserts dAMP and dGMP with low efficiency when situated opposite
78 adduct when C, A, or T was 5' to the lesion; dAMP and dTMP were misincorporated at a frequency of 2-4
79 d(Tp1meApT) and even 5'-phosphorylated 1-me-dAMP were relatively efficiently demethylated, and compe
81 ytic subunit alone primarily misincorporated dAMP and dGMP opposite the BaP DE-dG adducts, and incorp
82 protonated forms of the four mononucleotides dAMP, dCMP, dGMP, and dTMP was studied experimentally by
85 The incorporation of the correct nucleotide, dAMP, by hpol eta opposite cross-linked T was 3-5-fold m
86 enow fragment promotes blunt end addition of dAMP; this reaction was much less efficient than inserti
87 Synthesis starts with aminoethylation of dAMP and continues with rearrangement of N(1)-(2-aminoet
90 dG lesion, accompanied by a small amounts of dAMP and dTMP incorporation and one- and two-base deleti
91 tions catalyzed by pol eta, small amounts of dAMP misincorporation and one-base deletions were detect
92 the biphasic pre-steady-state time course of dAMP insertion opposite an abasic site which indicates t
93 has been tested in the dephosphorylation of dAMP, the hydrolysis of oligo(A)12, and the oligomerizat
94 mechanism of dimer bypass, the efficiency of dAMP and pyrene nucleotide insertion opposite the thymin
95 e found that the incorporation efficiency of dAMP opposite dT decreased 10(2)-10(3)-fold even when on
96 cus kodakaraensis catalyzed the formation of dAMP and two products that were identified as dAMP-glyce
104 adduct promote significant incorporation of dAMP and lesser amounts of dTMP opposite the lesion.
107 ntributing to the selective incorporation of dAMP in full-length products was preferential extension
109 e-directed 8-oxo-dG lesion, incorporation of dAMP opposite 8-oxo-dG was slightly favored over dCMP de
110 ule" reflects the preferred incorporation of dAMP opposite abasic lesions in Escherichia coli in vivo
113 ceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence
114 e cross-links resulted from incorporation of dAMP opposite the template base, in agreement with the s
118 y encode almost exclusively the insertion of dAMP and dGMP (encoding G --> T and G --> C transversion
119 f nucleotide incorporation; the insertion of dAMP and dGMP was favored over that of the correct nucle
123 tate kinetic parameters for the insertion of dAMP opposite dT using primer/templates (P/T)-containing
124 We proposed that indiscriminate insertion of dAMP opposite the 3'-T of each photoproducts takes place
125 lts suggest that the error-free insertion of dAMP opposite the 3'-T of the cis-syn thymine dimer happ
126 utside the active site, whereas insertion of dAMP opposite the 5'-T takes place with the photoproduct
128 In contrast to the biphasic insertion of dAMP, pre-steady-state time courses for the insertion of
132 substantial frequency of misincorporation of dAMP opposite N(6)-CMdA and, to a lesser extent, misinse
136 complementary dNMP decreases in the order of dAMP > dGMP > dTMP > dCMP, from a high of 5.8 when dAMP
140 tide incorporation; the insertion of dGMP or dAMP was slightly favored over the insertion of the corr
142 nucleotide incorporation followed the order: dAMP > dGMP > dTMP > dCMP, which did not correlate with
145 the exo(-)Klenow fragment of DNA polymerase, dAMP (22%), TMP (16%), dGMP (5.3%) and dCMP (1.2%) were
153 the nontemplating nature of the abasic site, dAMP is often preferentially inserted opposite the lesio
154 critical for the stability of the correct T.dAMP base pair when the 5'-CTGG sequence is present in t
157 yme was no longer able to excise 3'-terminal dAMP from a freshly added normal 21-mer annealed to M13m
158 rene nucleotide with greater efficiency than dAMP opposite the 3'-T of an undimerized or dimerized T
160 etic parameters of incorporation showed that dAMP was inserted opposite Ug more efficiently than oppo
161 imase-coupled pol alpha activity showed that dAMP was preferentially incorporated opposite the abasic
162 e two calcium ions of SAP are bridged by the dAMP phosphate group and five hydrogen bonds are formed
163 by electrostatic interactions involving the dAMP phosphate groups; decamerization buries 1000 A2 (2.
164 B69pol) prefers to insert dCMP as opposed to dAMP when situated opposite 8-oxoG by >2 orders of magni
165 echanism, dPMP was inserted in preference to dAMP opposite the 3'-T of all the photoproducts with the
167 dGMP > dTMP > dCMP, from a high of 5.8 when dAMP is to be inserted following a T to a low of 0.5 whe
168 requency of chain extension was highest when dAMP was positioned opposite a natural abasic site.
169 xception of the trans,syn-I product, whereas dAMP was inserted in preference to dPMP opposite the 5'-
170 ion of a series of complex products in which dAMP is incorporated opposite M(1)dG in the 3'-GXT-5' te
171 We recently proposed a mechanism for why dAMP is primarily inserted opposite both T's of photopro
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