ライフサイエンスコーパス: dATP

1 dATP insertion opposite M(1)dG was the most favored inse

2 dATP levels were normalized by treatment with either car

3 dATP was highly elevated in ADA-deficient cultures, and

4 sed with the dATP/ATP ratio (p < 0.01 at 10% dATP).

5 yl conformation (versus 3'-exo in 3'-MANT-2'-dATP).

6 near, with the nonlinearity evident at 2-20% dATP; force increased significantly with only 10% of sub

7 PAPgamma bound to cordycepin triphosphate (3'dATP) and Ca(2+).

8 pre-steady-state kinetic analysis using a 3'-dATP analogue in place of ATP shows that removal of this

9 ibitor (Ki, 10.7 nM), followed by 2'-MANT-3'-dATP [2'-O-(N-methylanthraniloyl)-3'-deoxy-ATP] (Ki, 16.

10 Reasonable binding modes of 2'-MANT-3'-dATP and bis-(M)ANT-nucleotides at sGC alpha1beta1 requi

11 amma-CXN(3) dGTP (5-6) and alpha,beta-CXN(3) dATP (7-8) analogues are described.

12 Labeling with [alpha-32P]dATP followed by a cold dATP chase has demonstrated that

13 eoxyadenosine-5'-O-(1-thiotriphosphate) as a dATP alternative during DNA strand extension.

14 Apaf-1 contains a dATP as a cofactor.

15 t bases ranges from a low of 25 microM for a dATP:A mispair to a high of 360 microM for a dCTP:T misp

16 values for the insertion of dATP opposite A (dATP/A) as well as dATP/G and dGTP/G were decreased grea

17 des including A:dCTP, A:dGTP, A(syn):dGTP, A:dATP, A(syn):dATP, T:dCTP, and T:dGTP to study the struc

18 syn):dATP<T:dCTP<A:dGTP<A(syn):dGTP<A:dCTP<A:dATP.

19 y which pol X can preferentially accommodate dATP opposite template oxoG further underscores the role

20 uently elongated by DNA polymerase and added dATP.

21 transcription completely dependent on added dATP.

22 ican swine fever virus incorporates adenine (dATP) opposite to oxoG with higher efficiency than the n

23 That hpol eta discriminates against dATP exclusively at the insertion stage is confirmed by

24 ion between active-site residue Tyr(271) and dATP that causes an anti to syn change in the 8-oxoG (sy

25 mutant RecA protein also hydrolyzes ATP and dATP at rates up to 10-fold higher than either single mu

26 Moreover, ATP and dATP bind at an additional allosteric site 40 A away fro

27 We determined that ATP and dATP bind to NrdR in a negatively cooperative fashion, s

28 Activity assays show that ATP and dATP, but not ADP or AMP, bind to the processed Csp9 p35

29 ity of the enzyme was only fueled by ATP and dATP.

30 In the presence of cytochrome c and dATP, Apaf-1 assembles into an oligomeric apoptosome, wh

31 Upon binding to cytochrome c and dATP, Apaf-1 oligomerizes into a heptameric complex know

32 the molecular mechanism of cytochrome c- and dATP-mediated activation of Apaf-1.

33 had 100-fold higher dATP concentrations and dATP/TFV-DP ratios, likely reflecting the activated stat

34 h on the incorporation of dCTP (correct) and dATP (incorrect) opposite the G (normal) or 8-oxoG(damag

35 ations in association with elevated dAdo and dATP levels and increased apoptosis in the lung.

36 similar insertion efficiencies for dCTP and dATP.

37 With ddCTP, dCTP, and dATP the phosphodiester bonds were formed even in the pr

38 NSAH depresses dGTP and dATP levels in the dNTP pool causing S-phase arrest, pro

39 F1143 and the protein bound to both dGTP and dATP suggested allosteric regulation of its enzymatic ac

40 d that the Dpo4 polymerase inserted dGTP and dATP when challenged by the PdG adduct.

41 polymerases efficiently polymerize dGTP and dATP when tC and tCo are in the template strand.

42 e-primer DNA reveal non-productive (dGTP and dATP) alignments of incoming nucleotide and 8-oxoG.

43 els of DNA precursors, particularly dGTP and dATP.

44 more than 4-fold normal, and dCTP, dGTP, and dATP concentrations rose 1-2 times normal.

45 Native human DNA polymerase eta, DNA and dATP were co-crystallized at pH 6.0 without Mg(2+).

46 erent rotameric conformations in TFV-DP- and dATP-bound structures that may help explain how K65R RT

47 Levels of dTTP and dATP were significantly reduced in cls8.

48 ansfer other nucleotides (UTP, CTP, GTP, and dATP) to the acceptor RNA in the absence or presence of

49 n to incorporate both dCTP (no mutation) and dATP (G-->T substitution) opposite 8-oxo-G.

50 ation of a Hoogsteen pair between 8-oxoG and dATP less favorable.

51 , dATP insertion opposite an abasic site and dATP misinsertions have common features.

52 ined by reduced levels of the R2 subunit and dATP in p53-deficient cancer cells.

53 gen-bonding interactions between the TTD and dATP than between the TTD and dGTP.

54 ty than ATP, PSTK utilizes GTP, CTP, UTP and dATP as phosphate-donors.

55 oG:anti-dCTP) and Hoogsteen (syn-8-oxoG:anti-dATP) base pairing were clearly visible and were maintai

56 present at fertilization and is activated as dATP is depleted via DNA polymerization.

57 -diphosphate shows a similar binding mode as dATP in the nucleotide-binding site.

58 significantly with only 10% of substrate as dATP.

59 rtion of dATP opposite A (dATP/A) as well as dATP/G and dGTP/G were decreased greater than 10-fold wi

60 Substitution of 2'-deoxy ATP (dATP) for ATP as substrate for actomyosin results in sig

61 ted that cardiac myosin can use 2-deoxy-ATP (dATP) as an energy substrate, that it enhances contracti

62 Replacing ATP with 2 deoxy-ATP (dATP) increased F-actin speed for both groups by a simil

63 Here we show that 2'-deoxy-ATP (dATP), but not 3'-deoxy-ATP, increases the activity of G

64 eoxyribose leads to ligands (mant-deoxy-ATP [dATP], mant-deoxy-ADP) with inverse agonist activity.

65 monstrate that purified cryopyrin binds ATP, dATP, and ATP-agarose, but not CTP, GTP, or UTP, and exh

66 tch, whereas the composite s-site binds ATP, dATP, dTTP, or dGTP and determines which substrate to re

67 igD and its complexes with manganese and ATP/dATP substrates, which reveal a minimized polymerase wit

68 Apaf-1 binds to and hydrolyses ATP/dATP and their analogues.

69 nzyme inhibition based on differences in ATP/dATP consumption.

70 s to cytochrome c and in the presence of ATP/dATP forms an apoptosome, leading to the recruitment and

71 although the Q24A mutant still relied on ATP/dATP for duplex unwinding.

72 P) from the analogous 5'-triphosphate (2'-Az-dATP) onto the tyrosine hydroxyl group of a peptide, whi

73 lar to those observed in the binary Pol beta-dATP complex.

74 ight into the discrimination by Dpo4 between dATP and dGTP opposite DFT and its inability to extend b

75 on the go and that negative feedback between dATP and ribonucleotide reductase ensures tight control

76 2+) directly bridges the interaction between dATP and HBV-RT and the other serves as a coordinator to

77 ed protein lacking this cone could only bind dATP to its s-site.

78 in an N-terminal ATP cone domain that binds dATP or ATP and functions as an on/off switch, whereas t

79 mined using DNA polymerase I-mediated biotin-dATP nick translation (PANT) labeling.

80 ed 2'-deoxyadenosine 5'-triphosphate (biotin-dATP) by terminal deoxynucleotidyl transferase (TdT).

81 in close proximity to the sugar of the bound dATP, whereas Arg-504 makes a hydrogen bridge with the b

82 uctures of the T7 helicase domain with bound dATP or dTTP identified Arg-363 and Arg-504 as potential

83 ive of non-productive complex formation, but dATP:8-oxoG insertion events that do occur are 2-fold mo

84 ts by enhancing allosteric RNR inhibition by dATP.

85 tly compelling to consider elevated cardiac [dATP] as a therapeutic option to treat systolic dysfunct

86 g-term (at least 5-mo) elevation of cardiac [dATP] results in sustained elevation of basal left ventr

87 strate/specificity effector-pairs bound (CDP/dATP, UDP/dATP, ADP/dGTP, GDP/TTP) that reveal the confo

88 ccurs with only minor elevation of cellular [dATP].

89 ling with [alpha-32P]dATP followed by a cold dATP chase has demonstrated that newly synthesized DNA o

90 ease in the specificity constant for correct dATP:T incorporation.

91 300-fold slower incorporation of the correct dATP:T relative to that of the wild type led to a 130-fo

92 The method employs Cy5-dATP incorporation into a DNA primer that has been prela

93 In the presence of cytochrome c (CytC), dATP or ATP binds to Apaf-1 and triggers heptamerization

94 However, RecA protein is also a dATPase; dATP supports RecA-RFP nucleoprotein filament formation

95 results suggest that activating the dAdo-DCK-dATP pathway directly results in increased apoptosis in

96 te base opposite to the incoming dNTP (dCTP, dATP, dGTP) is oxoG.

97 used as substitutes for natural dTTP, dCTP, dATP, and dGTP in PCR.

98 ry hpol eta-DNA complexes and incoming dCTP, dATP, or dGTP opposite 8-oxoG reveal that an arginine fr

99 dition, the rate of incorporation of 1-deaza-dATP opposite guanine was decreased 5-fold.

100 Eliminating N(6) from 3-deaza-dATP now greatly reduced incorporation, suggesting that

101 repared by thiol addition to 7-vinyl-7-deaza-dATP.

102 inhibited by endogenous levels of deoxyATP (dATP) present at fertilization and is activated as dATP

103 second, and among natural deoxynucleotides, dATP was the preferred substrate due to its stronger int

104 with mixtures of nucleotides, including dGTP/dATP, dGTP/dCTP, dGTP/dTTP, and dGTP/dUTP.

105 DNA polymerase beta (pol beta) discriminates dATP from dCTP when processing 8-oxoguanine (8-oxoG), we

106 ructures were observed in a ternary Dpo4-DNA-dATP complex and a ternary Dpo4-DNA-ddATP complex, with

107 e we report crystal structures of RT-RNA/DNA-dATP and RT-RNA/DNA-nevirapine (NVP) ternary complexes a

108 The polymerase region of RT-RNA/DNA-dATP complex resembles DNA/DNA ternary complexes apart f

109 , and GDP, C site) as well as ATP and dNTPs (dATP, dGTP, TTP) allosteric effectors that control enzym

110 e presence of the negative activity effector dATP and provide structural support for an active alpha(

111 characterized RNRs, the binding of effector dATP alters the active site to select for pyrimidines ov

112 Enzyme hydrolyzed all rNTPs efficiently, dATP and dCTP with moderate efficiency, while it showed

113 c studies indicated that the first blunt-end dATP incorporation was 80-fold more efficient than the s

114 we report the effect of chronically enhanced dATP concentration on cardiac function using a transgeni

115 In nearly 10% of events, dATP would be incorporated, and once incorporated dA (op

116 which is subsequently replaced by exogenous dATP.

117 bound nucleotide (ATP and to a lesser extent dATP) might dictate the requirement for accessory factor

118 the presence of ATP and, to a lesser extent, dATP.

119 ed dGTP, whereas Taq demonstrated a bias for dATP.

120 state kinetics shows diminished "bursts" for dATP:8-oxoG and dCTP:8-oxoG incorporation, indicative of

121 tudies, abasic sites preferentially code for dATP insertion (the "A rule").

122 but with a nearly 2-fold longer duration for dATP or dTTP incorporation than for dCTP or dGTP into co

123 The apparent Km for dATP at site 2 is approximately 10 mum for mouse and 1 m

124 an abasic site, with kinetic preference for dATP as the substrate.

125 260-2900-fold and exhibited a preference for dATP incorporation.

126 suggest that the significant preference for dATP insertion observed experimentally can result from t

127 ts affinity for dsDNA and its preference for dATP over ATP.

128 results show that Arg-363 is responsible for dATP, dCTP, and dGTP hydrolysis, whereas Arg-504 and Ser

129 otential determinants of the specificity for dATP and dTTP.

130 ited incorporation, whereas removing N3 from dATP dramatically increased incorporation (32-fold).

131 Similarly, syn-Fapy.G:dATP pairing showed greater stacking in the 5'-TGT seque

132 In pol beta, the syn-8-oxo-G:dATP pair showed greater stacking with the 3'-T:A base p

133 ld higher incorporation efficiency of dCTP > dATP opposite 8-oxoG and 4-fold higher efficiency of ext

134 rimers showed >/=95% incorporation of dCTP > dATP opposite 8-oxoG.

135 catalytic core tetramer in complex with GTP/dATP, revealing the structural basis of GTP-mediated SAM

136 with 1 mm deoxyadenosine accumulates higher dATP levels than mammalian cells but that this effect di

137 ssue, rectal lymphocytes had 100-fold higher dATP concentrations and dATP/TFV-DP ratios, likely refle

138 lity and obtain greater understanding of how dATP substitution results in contractile enhancement, we

139 rison with autoinhibited Apaf-1 revealed how dATP binding triggers a set of conformational changes th

140 complexes revealed incoming non-hydrolyzable dATP or dGTP analogs not pairing with but instead in a s

141 Our results identify dATP/TFV-DP ratios as a possible correlate of protection

142 alog combining the chemical modifications in dATP and P-ATP.

143 -3', a diffracting ternary complex including dATP was obtained.

144 aged in a nascent base pair with an incoming dATP analog.

145 - N (2)-dG ([BP]G*), reveal that an incoming dATP is significantly preferred over the other three dNT

146 complex of the R283K mutant with an incoming dATP-analogue and templating 8-oxoG resembles a G-A mism

147 rming a Hoogstein base pair with an incoming dATP.

148 binding affinity for the incorrect incoming dATP opposite 8-oxoG.

149 preventing Gh from pairing with the incoming dATP or dGTP base.

150 ue interaction between 5ClC and the incoming dATP, which would facilitate mutagenic lesion bypass.

151 s show that both Dpo4 and Poleta incorporate dATP opposite the 5' T of the CPD via Watson-Crick base

152 analysis, hpol eta preferred to incorporate dATP and dGTP, compared with dTTP.

153 BF incorporates dATP opposite 8-oxoG less efficiently, and modifying N1,

154 NA synthesis by preferentially incorporating dATP opposite the noninstructional lesion.

155 al closing for correct dCTP versus incorrect dATP incoming nucleotide opposite a template G.

156 DNA polymerases insert dATP opposite the oxidative damage product 7,8-dihydro-8

157 mpanied by the accumulation of intracellular dATP.

158 hen only 10% of the contractile substrate is dATP.

159 s efficient base stacking between the lesion:dATP pair and the 3'-A:T base pair in the 5'-TGA sequenc

160 f the cell cycle and preferentially lowering dATP levels.

161 n an error-prone fashion by inserting mainly dATP.

162 Inverse agonist activity of mant-dATP was found at the WT human P2Y(12) and half of the c

163 Thus, N-MC-dATP corrects the error-prone nature of hpol iota by pre

164 ternary complex of hpol iota inserting N-MC-dATP opposite dT reveals that the adenine ring is stabil

165 nocarba-2'-deoxyadenosine triphosphate (N-MC-dATP).

166 less catalytically favorable than anti N-MC-dATP.

167 Unmodified dATP and South-MC-dATP each adopt syn glycosyl orientations to form Hoogst

168 with the natural dATP substrate, we measured dATP contents in peripheral lymphocytes, lymphoid tissue

169 that modified nucleotides such as N6-methyl-dATP and O6-methyl-dGTP are incorporated opposite an aba

170 incorporation of matched dCTP or mismatched dATP.

171 At concentrations greater than 1 mM, dATP or ATP also functions as a negative regulator of ap

172 olymerization product with the thio-modified dATP as a substrate.

173 transcription by competing with the natural dATP substrate, we measured dATP contents in peripheral

174 he applicability of the N(6)-(2-nitrobenzyl)-dATP as an ideal RT for CRT sequencing.

175 The 3'-OH group of the N(6)-(2-nitrobenzyl)-dATP remains unblocked, providing favorable incorporatio

176 omplex) and with an incoming nonhydrolyzable dATP analog (ternary complex) were solved.

177 ry is hampered when the incorrect nucleotide dATP is bound opposite 8-oxoG; the unfavorable interacti

178 depending on the availability of nucleotide dATP/ATP.

179 vely), and competing endogenous nucleotides (dATP and dCTP) in 47 healthy women.

180 In the absence of dATP or ATP, Dronc zymogen potently induces formation of

181 ytidine kinase prevented the accumulation of dATP and restored thymocyte differentiation and prolifer

182 osis as a consequence of the accumulation of dATP derived from thymocytes failing the positive/negati

183 ers and hexamers, was induced by addition of dATP or dGTP, but not of dTTP or dCTP.

184 f hpol eta with a non-hydrolyzable analog of dATP or dGTP opposite an abasic site, H-bonding was obse

185 f the R2 subunit extends the availability of dATP in the G(2)/M phase to promote the repair of NER-me

186 he calculations indicate that the binding of dATP to the enzyme-DNA complex is thermodynamically favo

187 osphate to mimic the active-site contacts of dATP may explain its effective inhibition of RT and main

188 s an effector, activating the degradation of dATP and dCTP with dGTP also being consumed in the react

189 is accompanied by simultaneous depletion of dATP pools (through ribonucleotide reductase inhibition)

190 Frequencies of dATP misinsertion and extension beyond mispairs were als

191 es the energy derived from the hydrolysis of dATP in addition to dTTP for mediating DNA unwinding.

192 me c binding to Apaf-1 induces hydrolysis of dATP to dADP, which is subsequently replaced by exogenou

193 orporation, suggesting that incorporation of dATP (analogues) opposite 8-oxoguanine proceeds via a Ho

194 o the reduced efficiency of incorporation of dATP opposite T, and that the autosomal dominant phenoty

195 pol/ K d (dNTP) values for the insertion of dATP and dGTP opposite 7-deazaadenine and 7-deazaguanine

196 the k(pol)/K(d) values for the insertion of dATP opposite A (dATP/A) as well as dATP/G and dGTP/G we

197 bserved for hpol iota-catalyzed insertion of dATP opposite dT.

198 of deoxyadenosine accumulates high levels of dATP in an adenosine kinase-dependent process and dies w

199 ely small (but supraphysiological) levels of dATP increase the number of strongly attached, force-pro

200 Pols differ strikingly in the mechanisms of dATP incorporation, with Dpo4 incorporating opposite an

201 oxanthine base pairs by pol alpha, and N3 of dATP again helps prevent polymerization opposite a templ

202 imately 50% conversion) by polymerization of dATP using yeast PaP, and the short DNA strand appended

203 In the presence of dATP, glycerol, and Tris buffer, the DNA primase isolate

204 bled a large Dark complex in the presence of dATP.

205 olysis of dTTP without affecting the rate of dATP hydrolysis.

206 To determine the minimal ratio of dATP/ATP (constant total NTP) that significantly enhance

207 in of Y115 more than does the ribose ring of dATP, possibly picking up a favorable pi-pi interaction.

208 We used a series of dATP and dGTP analogues to determine how DNA polymerase

209 r NTPs, resulting in the preferential use of dATP and the use of dCTP, a nucleotide not normally used

210 Delivery of 8-oxo-dGTP and 2-OH-dATP to zebrafish embryos was highly toxic in the absenc

211 At pH 6.5, only dATP was inserted, but as the pH increased to 9.0, the a

212 ite 2 and activate SAMHD1, but in cells only dATP or dTTP are present at sufficient concentrations.

213 nzymes, that monitors the decrease of ATP or dATP in real time, allowing detection of enzyme inhibiti

214 ATP or dATP is a required activator of Apaf-1 for formation of

215 in vitro with the addition of CC and ATP or dATP to cell lysates.

216 ed that Mtr4p can, in the presence of ATP or dATP, unwind the duplex region of a partial duplex RNA s

217 nt protein can bind but not hydrolyze ATP or dATP.

218 ty was dependent upon the presence of ATP or dATP.

219 g DNA helicase activity that required ATP or dATP.

220 rotein filaments but do not hydrolyze ATP or dATP.

221 presence of adenosine triphosphate (ATP) or dATP whereas the non-hydrolyzable gamma-S-ATP does not s

222 not modulate a preference for either dCTP or dATP when opposite 8-oxodG in single-nucleotide gapped D

223 g sites, site 1 binding dGTP, site 2 dGTP or dATP.

224 n orientation whether GS-9148-diphosphate or dATP is bound.

225 The binding of either GS-9148-diphosphate or dATP to the binary RT-DNA complex resulted in the finger

226 lexes with tenofovir diphosphate (TFV-DP) or dATP.

227 Dpo4 capable of incorporating dCTP, dTTP or dATP opposite the adduct reasonably well.

228 ATP but not ADP, AMP, GTP, or dATP specifically protected Hbr1p from proteolysis by tr

229 e beta preferentially incorporated dCTP over dATP, DNA polymerase lambda did not modulate a preferenc

230 ATP, while polymerase alpha favors dGTP over dATP by a factor of 30-65.

231 al substrates (8-oxo-dGTP, dUTP, dITP, 2-oxo-dATP), which allows them to select these substrates from

232 cture of the L561A variant forming an 8-oxoG.dATP mispair and show that the propensity for forming th

233 The new analog P-dATP can be not only an invaluable tool to study CFTR ga

234 hesized N(6)-(2-phenylethyl)-2'-deoxy-ATP (P-dATP), an analog combining the chemical modifications in

235 analogue, N6-(2-phenylethyl)-2'-deoxy-ATP (P-dATP), can increase the open probability (Po) to approxi

236 Interestingly, P-dATP completely rectified the gating abnormality of Delt

237 tion effect, suggesting that the action of P-dATP takes place at the interface of both NBDs.

238 We show that P-dATP binds to ABP1 to potentiate the activity of G551D,

239 ctivation of RNR upon commitment to S phase, dATP feedback inhibition ensures that the dNTP concentra

240 strongly discriminated against polymerizing dATP opposite 8-oxoG, and removing N1, N(6), or N7 furth

241 experimentally can result from two possible dATP incorporation modes.

242 it is noteworthy that Dpo4 strongly prefers dATP opposite DFT over dGTP (approximately 200-fold) and

243 TP activates the enzyme solely by preventing dATP from binding.

244 In principle, dATP feedback inhibition should be sufficient to couple

245 alogues (dGTP-PC-Bodipy-FL-510, dUTP-PC-R6G, dATP-PC-ROX, and dCTP-PC-Bodipy-650) (PC, photocleavable

246 In contrast, RecA . dATP efficiently catalyzes strand exchange even in the a

247 cleotide excision repair (NER) and a reduced dATP level in the G(2)/M phase of the cell cycle.

248 48R and E96D single mutant proteins restores dATP hydrolysis to 25% of the wild type rate, with maxim

249 l the atomistic structures of HBV-RT/DNA-RNA/dATP and HBV-RT/DNA-RNA/TFV-DP (tenofovir diphosphate) c

250 dynamics (MD) simulations of HBV-RT/DNA-RNA/dATP complexes revealed strong coupling of the natural n

251 ukaryotic and Escherichia coli class I RNRs, dATP inhibits enzyme activity through the formation of i

252 dependent complex with RNR, which stabilizes dATP in the activity site of RNR and thus inhibits the e

253 more efficiently than the natural substrate, dATP.

254 oupling of the natural nucleotide substrate, dATP, to the active site of the RT, and the differential

255 e system insertions by pol mu: T:dGTP<A(syn):dATP<T:dCTP<A:dGTP<A(syn):dGTP<A:dCTP<A:dATP.

256 A:dCTP, A:dGTP, A(syn):dGTP, A:dATP, A(syn):dATP, T:dCTP, and T:dGTP to study the structure-function

257 es an anti to syn change in the 8-oxoG (syn):dATP complex explains this slow motion, in contrast to t

258 eading to predominantly single non-templated dATP incorporation.

259 dGTP with a 4-9-fold higher probability than dATP, while polymerase alpha favors dGTP over dATP by a

260 Here we demonstrate that dATP or ATP may have an additional role in controlling A

261 The calculations also illustrate that dATP binding is thermodynamically favored over dGTP bind

262 Furthermore, the simulations indicate that dATP and dTTP are better incorporated in the damaged sys

263 bserved, steady-state kinetic data show that dATP insertion opposite DFT is greatly inhibited relativ

264 The dATP can be inserted opposite the T on the 5' side of th

265 The dATP did not pair with PdG, but instead with the 5'-neig

266 The dATP hydrolysis and exchange on Apaf-1 are two required

267 The dATP-induced inactive form is an alpha4 complex, which c

268 The dATP/dGTP insertion ratio opposite the dGh/dIa site as a

269 r ATPase activity than that of wt ABCG2, the dATP-dependent methotrexate transport activities of thes

270 In addition, the dATP can be misincorporated opposite the adduct.

271 These structures reveal that binding of the dATP analog induces a closed polymerase conformation, an

272 Examination of the active site with the dATP substrate suggests an in-line nucleophilic attack o

273 in sliding speed linearly increased with the dATP/ATP ratio (p < 0.01 at 10% dATP).

274 Each alpha polypeptide binds three dATP molecules, and the N-terminal ATP cone is critical

275 Thus, dATP insertion opposite an abasic site and dATP misinser

276 tive concentration (EC90) ratios of TFVdp to dATP and FTCtp to dCTP (alone and in combination) for pr

277 th incorrect deoxyadenosine 5'-triphosphate (dATP).

278 to incorporate deoxyadenosine triphosphate (dATP) at the 3'-OH of an RNA molecule, followed by termi

279 phosphate or 2'-deoxyadenosine triphosphate (dATP), and a post-incorporation structure with GS-9148 t

280 -position of 2'-deoxyadenosine triphosphate (dATP), which, upon incorporation, terminates DNA synthes

281 n IRBIT forms a deoxyadenosine triphosphate (dATP)-dependent complex with RNR, which stabilizes dATP

282 osphorylated to deoxyadenosine triphosphate (dATP).

283 a free deoxyribose nucleotide triphosphate, dATP or dGTP, to Pol eta complexed with undamaged or dam

284 cificity effector-pairs bound (CDP/dATP, UDP/dATP, ADP/dGTP, GDP/TTP) that reveal the conformational

285 Unmodified dATP and South-MC-dATP each adopt syn glycosyl orientati

286 esults in contractile enhancement, we varied dATP/ATP ratio in porcine cardiac muscle preparations.

287 de conformations and contacts for ATP versus dATP are observed in the cocrystals, the functional anal

288 t form in the presence of ATP but do so when dATP is provided.

289 complex in MCF-7 cell lysates activated with dATP/cytochrome c.

290 Hoogsteen edge, which can hydrogen-bond with dATP or dGTP.

291 crystals permit substitution of TFV-DP with dATP at the dNTP-binding site.

292 P is proficient for DNA strand exchange with dATP or at lower pH.

293 is on a dT template) that were extended with dATP by Escherichia coli DNA polymerase I.

294 tension redevelopment (k(TR)) increased with dATP at all Ca(2+) levels.

295 k(TR) increased linearly with dATP/ATP ratio at pCa 4.5 and 5.5.

296 .5), isometric force increased linearly with dATP/ATP ratio, but at submaximal Ca(2+) (pCa 5.5) this

297 nsertion complexes for the Y567A mutant with dATP and dCTP opposite a templating 8-oxoG in a 13/18mer

298 einsertion complex for the Y567A mutant with dATP opposite a templating Gh (R-configuration) in a 13/

299 GTP also being consumed in the reaction with dATP.

300 ions before and after the chemical step with dATP and dCTP opposite an 8-oxoG template started from p