ライフサイエンスコーパス: dT

1 dT formed no cross-links, nor did Arg, Gln, Tyr, or Asn.

2 assessed two therapies in Tk2(-/-) mice: (1) dT+dC and (2) coadministration of the deaminase inhibito

3 ded DNA conjugates with the sequence (dA)10.(dT)10 and hexaethylene glycol linkers at one end (hairpi

4 ntervening residues in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered through MD simul

5 e ( approximately 7-7.5) with poly(dA).2poly(dT) as compared to neomycin ( approximately 6.5).

6 perature (T(m3-->2)) at which poly(dA).2poly(dT) dissociated into poly(dA).poly(dT) and poly(dT) incr

7 y(dT) DNA duplex than for the poly(dA).2poly(dT) DNA triplex.

8 ty of all tested ligands with poly(dA).2poly(dT) increased in the following order: neomycin < 1 < 3 <

9 lator-neomycin conjugates for poly(dA).2poly(dT) increases as a function of the surface area of the i

10 the increment of T(m3-->2) of poly(dA).2poly(dT) induced by neomycin was negligible under the same co

11 r conjugates (1-4) stabilized poly(dA).2poly(dT) much more than its parent compound, neomycin.

12 y to neomycin, 3 destabilizes poly(dA).2poly(dT) triplex but stabilizes poly(dA).poly(dT) duplex, sug

13 -neomycin conjugates (1-4) to poly(dA).2poly(dT) was also confirmed by competition dialysis and a flu

14 binding of compounds 1-4 with poly(dA).2poly(dT) was mostly enthalpy-driven and gave negative DeltaC(

15 0.3) x 10(8) M(-1)] of 2 with poly(dA).2poly(dT) was the highest, almost 1000-fold greater than that

16 ator-neomycin conjugates with poly(dA).2poly(dT) were derived from an integrated van't Hoff equation

17 a DNA polynucleotide triplex [poly(dA).2poly(dT)] were conducted.

18 tL dimer binds to an 18 bp duplex with a 3'-(dT(20)) single-stranded flanking region, with apparent a

19 RecBC or RecBCD to a DNA end with only a 3'-(dT)6 tail).

20 ing noncomplementary twin 5'-(dT)(6) and 3'-(dT)(6) tails, and unwinding can be described by a simple

21 il is extended to 10 nt [5'-(dT)(10) and 3'-(dT)(6)], the DNA end structure for which RecBCD displays

22 DNA, RecBCD unwinding of DNA possessing 3'-(dT)(6) and 5'-(dT)(6) noncomplementary ssDNA tails is we

23 ementary single-stranded (ss) DNA tails [3'-(dT)(6) and 5'-(dT)(6) or 5'-(dT)(10)] on the mechanism o

24 ned to test a previous proposal that the 3'-(dT)n tail can be looped out upon binding RecBC and RecBC

25 However, placing PEG at the end of the 3'-(dT)n tail increases the binding affinities to their maxi

26 xth and the final two nucleotides of the 3'-(dT)n tail.

27 ults showed that BrdU replaced more than 30% dT in genomic DNA after the cells were treated with 10 m

28 reases polymerase activity of gp5 on dA(350)/dT(25).

29 the HB pattern and base pair geometry of 3DA/dT is exactly the same as those of dA/dT, which makes 3D

30 d selectively to the PPT near the 5'(rA)(4):(dT)(4) tract and the 3' PPT-U3 junction.

31 winding of DNA possessing 3'-(dT)(6) and 5'-(dT)(6) noncomplementary ssDNA tails is well described by

32 -stranded (ss) DNA tails [3'-(dT)(6) and 5'-(dT)(6) or 5'-(dT)(10)] on the mechanism of RecBCD and Re

33 tary 5' ssDNA tail is extended to 10 nt [5'-(dT)(10) and 3'-(dT)(6)], the DNA end structure for which

34 DNA tails [3'-(dT)(6) and 5'-(dT)(6) or 5'-(dT)(10)] on the mechanism of RecBCD and RecBC unwinding

35 y of RecBC for DNA ends possessing 3' or 5'-(dT)(n) ssDNA tails with n<6 nt, with the relative enhanc

36 DNA end possessing noncomplementary twin 5'-(dT)(6) and 3'-(dT)(6) tails, and unwinding can be descri

37 After the addition of a dT residue to the DNA primer, which is specified by the

38 rs (preferentially initiating synthesis on a dT template) that were extended with dATP by Escherichia

39 , demonstrating that both KWK6 and the above dT-mers are sufficiently short so that the CEE extends o

40 le in bypassing alpha-dC, alpha-dG and alpha-dT in vivo.

41 ha-dC and triggered T-->A mutation for alpha-dT.

42 One of these dNTP analogs (dT) was demonstrated to fit into DNA polymerase beta (DN

43 es has revealed that the insertion of dA and dT was catalyzed by different polymerases in cells.

44 d tendency of hpol eta to insert both dC and dT opposite the O(6)-MeG lesion with similar efficiencie

45 X-ray crystal structures of both dC and dT paired with O(6)-MeG were solved in both insertion an

46 ed as a contaminant in the commercial dG and dT 3'-monophosphate samples.

47 During preparation of the dG and dT derivatives of alpha,beta-methylene diphosphate, we a

48 es in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered through MD simulation and free en

49 l conduction to this model altered %dLV and %dT to 13.1% and 10.1%, respectively.

50 lar-paced canine model resulted in %dLV and %dT values of -7.1% and 1.5%, in contrast to the experime

51 cardiac anatomy is responsible for %dLV and %dT values of 7.4% and 5.5%, respectively.

52 d as %dLV=100x(LVLATep-LVLATen)/LVLATep and %dT=100x(TLATep-TLATen)/TLATep, respectively).

53 showed a substantial proportion of apoptotic dT cells.

54 n of gal1 as well as periglandular apoptotic dT foci that colocalized with dNKs.

55 pha, beta)-imido]triphosphate (approximating dT) at both the insertion and extension stages with form

56 ave a more stable N-glycosidic bond (such as dT).

57 antly different from that of the cognate ATP/dT scaffold ( approximately 130-fold decrease in kpol).

58 e for UTP/dT incorporation compared with ATP/dT incorporation.

59 ino(bc-araT)-version of bicyclothymidine (bc-dT) has been achieved.

60 ine) and/or have a stable N-glycosylic bond (dT).

61 bstrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT)

62 When the opposing dA is replaced by dT, the activity of the adenine can be rescued by adding

63 ed to values expected from the slopes dB(c2)/dT approximately 2 T K(-1) near T(c), particularly at lo

64 an incorporate A, G, C, or T opposite the C5-dT-conjugated DNA-peptide conjugates, whereas human poly

65 rstanding how decidual CD8(+) T cell (CD8(+) dT) cytotoxicity is regulated and how these cells integr

66 However, CD8(+) dT degranulated, proliferated, and produced IFN-gamma, T

67 xpression analysis of effector-memory CD8(+) dT demonstrated a mixed transcriptional signature of T c

68 ance between transient dysfunction of CD8(+) dT that are permissive of placental and fetal developmen

69 the decidual microenvironment reduces CD8(+) dT effector responses to maintain tolerance to fetal ant

70 vitro stimulation, demonstrating that CD8(+) dT are not permanently suppressed and retain the capacit

71 form two hydrogen bonds with a complementary dT residue, is reported.

72 onucleotide contains fewer than 6 contiguous dT residues, the CspE interactions with single-stranded

73 stranded DNA containing 6 or more contiguous dT residues with high affinity (K(D) < 30 nM).

74 rature for the EXT monolayer, with dPi(crit)/dT approximately 1.5 mN/m/ degrees C, but the mixing pre

75 amounts of 3,5'-cyclo-dG (16) and 2,5'-cyclo-dT (17), respectively.

76 2x binding site (O1) on attL as well as a dA+dT-rich upstream element that is required for Orf2x inte

77 orporation of dCTP with templating bases dA, dT, and dC over correct dNTPs.

78 phabet, comprised of just two base pairs (dA-dT and dG-dC), is conserved throughout all life, and its

79 The poly (dA-dT) tracts affect but do not deplete nucleosomes in S. p

80 le-stranded DNA, such as poly(dA-dT)*poly(dA-dT) [poly(dA-dT)], can also induce IFN-beta, but the und

81 Here, we show that the cytosolic poly(dA-dT) DNA is converted into 5'-ppp RNA to induce IFN-beta

82 for synthesizing 5'-ppp RNA from the poly(dA-dT) template.

83 finity where the binding of HMGA2 to poly(dA-dT)(2) is enthalpy-driven and to poly(dA)poly(dT) is ent

84 B form double-stranded DNA, such as poly(dA-dT)*poly(dA-dT) [poly(dA-dT)], can also induce IFN-beta,

85 Biochemical analyses showed that poly(dA-dT)-activated AIM2 inflammasomes induce autophagy and IL

86 NA, such as poly(dA-dT)*poly(dA-dT) [poly(dA-dT)], can also induce IFN-beta, but the underlying mecha

87 i4) reveal that external binding to [poly(dA-dT)]2 becomes important when a fourth meso substituent i

88 uted porphyrin H2Tri4 combines with [poly(dA-dT)]2, [poly(dG-dC)]2, or salmon testes DNA.

89 ompetitive for a flexible host like [poly(dA-dT)]2.

90 natural base pair, and when combined with dA-dT and dG-dC, it provides a fully functional six-letter

91 Poly(dA.dT) DNA sequence elements are thought to promote transcr

92 e particle containing a 16 base-pair poly(dA.dT) element (A16 NCP) was investigated.

93 vivo and in vitro observations that poly(dA.dT) elements cause only modest changes in DNA accessibil

94 ed a decreased proportion of mutations at dA/dT and showed preferential RGYW/WRCY targeting by mutati

95 of 3DA/dT is exactly the same as those of dA/dT, which makes 3DA an optimal analogue for probing mino

96 The opening and the stability of each rU-dA/dT-dA base pair in the two structures are characterized

97 These results suggest that the central rU-dA/dT-dA base pairs in the adenine tract make the largest e

98 high transcription and long homopolymeric dA:dT tracts.

99 of a hydrogen bond for a halogen bond in dA:dT and dG:dC base pairs, which allows 1 or 2 hydrogen bo

100 production of IFN-beta triggered by poly (dA:dT) or HSV-1 requires IFNAR signaling.

101 t is particularly strong at repeated poly(dA:dT) and poly(dC:dG) tracts.

102 RSC clears promoters by translating poly(dA:dT) into directional nucleosome removal.

103 omplexes loaded onto the locus via a poly(dA:dT) tract in the gene promoter and mediated cohesion bef

104 f the PHO5 promoter that introduce a poly(dA:dT) tract-stimulated gene expression under nonpermissive

105 First, poly(dA:dT) tracts are localized in a strand-dependent manner, w

106 Second, poly(dA:dT) tracts are preferentially "capped" by G:C residues o

107 ng deserves to be singled-out: short poly(dA:dT) tracts are reported in the literature as fundamental

108 cross-linking, we show that internal poly(dA:dT) tracts do not block the engagement of the ATPase mot

109 el in which localized and G:C-capped poly(dA:dT) tracts initiate or facilitate the formation of NFRs

110 nd equilibrium experiments show that poly(dA:dT) tracts perturb remodeling reactions if within one an

111 racterized by positioned patterns of poly(dA:dT) tracts with several novel features.

112 /MARs are preferentially enriched in poly(dA:dT) tracts, sequences that resist nucleosome formation,

113 a closely knit relationship between poly(dA:dT) tracts, their capping patterns, and the central coor

114 tion at promoters is maintained over poly(dA:dT) tracts, whereas internucleosome spacing and all othe

115 , including lipid A, LPS, poly(I:C), poly(dA:dT), and cGAMP, induce cGAS expression in an IFN-I-depen

116 analyzing the dynamic expression of poly(dA:dT)-induced IFN-beta and cGAS transcripts, we have found

117 xpressed DNA sensor DDX41 attenuates poly(dA:dT)-triggered IFN-beta production and cGAS induction.

118 cients (using McMillan-Mayer theory), and dB/dT parameters have also been determined and discussed in

119 The dB/dT values suggest that L-glycine and beta-alanine act as

120 We observed that dC+dT delayed disease onset, prolonged life span of Tk2-def

121 al structures of N7mdG or dG paired with dC, dT, dG, and dA.

122 usly recognizes two decadeoxyoligothymidine (dT(10)) tracts to form triplexes with a peptide-DNA stra

123 DNA containing a template 2'-deoxythymidine (dT) paired with an incoming dNTP or modified nucleotide

124 nucleoside monophosphates to deoxythymidine (dT) and deoxycytidine (dC), we hypothesized that: (1) de

125 , deoxyuridylate (dU), and deoxythymidylate (dT), and have found that the macroscopic rate of translo

126 parison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerf

127 tion dispersion, we show here that wobble dG*dT and rG*rU mispairs in DNA and RNA duplexes exist in d

128 on dispersion recently showed that wobble dG.dT and rG.rU mismatches in DNA and RNA duplexes transien

129 in the order M(1)dG:dC > M(1)dG:dG > M(1)dG:dT approximately M(1)dG:dA, but neither hPol iota nor Re

130 precisely the mutagenic arrangement of dGTP:dT normally preferred by hpol iota.

131 hin pre-formed 3'-single-stranded (ss) DNA ((dT)n) tails was studied.

132 the rates of interannual tropical-mean dOLR/dTs and global-mean dP/dTs, consistent with the muted tr

133 e eclogite facies boundary has a positive dP/dT, cooling from supra-solidus conditions (T > 950 mascu

134 ee with the observation-based interannual dP/dTs all predict dP/dTs under global warming higher than

135 bal warming higher than the ensemble mean dP/dTs from the approximately 20 models analysed in this st

136 al tropical-mean dOLR/dTs and global-mean dP/dTs, consistent with the muted tropical high cloud shrin

137 tion-based interannual dP/dTs all predict dP/dTs under global warming higher than the ensemble mean d

138 n precipitation per unit surface warming (dP/dTs) for both interannual variability and global warming

139 ., no intervening residues in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered through M

140 the MM > PM probes had either a dThymidine (dT) or a dCytidine (dC) at the 13th position of the prob

141 trate a 50% reduction in action potential dV/dT, a 50-75% reduction in SCN5A, KCNJ2, and CACNA1C ion

142 t the 4' carbon (4'C-methyl dT and 4'C-ethyl dT).

143 ucleotide with 5-OH-dC, 5-propyne-dC, furano-dT, 1-(2'-deoxy-beta- d-ribofuranosyl)-2-oxo-7-deaza-8-m

144 tes were dC(6) approximately dA(6) > dG(6) > dT(6), correlating with the gas-phase acidities of nucle

145 atal macaque monkeys exposed to either [(3)H]dT or BrdU as embryos.

146 ers of DNA synthesis, [(3)H]thymidine ([(3)H]dT) and the later developed analog bromodeoxyuridine (Br

147 eplication marker tritiated thymidine ([(3)H]dT, or TdR) at early embryonic ages were killed at diffe

148 ore natural DNA constituent nucleotide [(3)H]dT.

149 3)H]-, [2'S-(3)H]-, [4'-(3)H]-, and [5'-(3)H]dTs provided values of 1.033 +/- 0.002, 1.004 +/- 0.002,

150 d eccentric remodeled anatomies resulted in %dT values of 15.6% and 1.3%, respectively.

151 d the glycosidic bond to syn and incorporate dT via a Hoogsteen pair.

152 wever, pol gamma preferentially incorporated dT opposite the gamma-HOPdA adduct and efficiently exten

153 ment of Arg104 by a smaller residue allows L-dT to bind deeper into the active site, making space for

154 arget nucleotides that are relatively large (dT and bromodeoxyuridine) and/or have a stable N-glycosy

155 ing a negative slope of their melting lines (dT/dP|coexist<0).

156 ligate poly-dT single-stranded DNA and long dT overhangs in a Ku- and XLF-independent manner, but no

157 (i.e., no intervening residues in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered throu

158 urations with either O(6)-MeG:dC or O(6)-MeG:dT bound compared with the corresponding situations in s

159 bose sugar ring at the 4' carbon (4'C-methyl dT and 4'C-ethyl dT).

160 uctures that form readily in unlinked (dA)n.(dT)n sequences, allowing the excited-state dynamics of s

161 On the other hand, the structure of N7mdG:dT shows that the mispair forms three hydrogen bonds and

162 an intercalated conformer, whereas the NarI-dT/-2 deletion duplex exists as multiple conformers.

163 lving the modified S-cdG.dC and 3'- neighbor dT.dA base pairs.

164 ne abolished TLS associated with dA, but not dT, insertion.

165 Arsenolysis of dT by hTP permits kinetic isotope effect (KIE) analysis

166 TDG cleaves the N-glycosylic bond of dT and some other nucleotides, including 5-substituted 2

167 alyzes a slow hydrolytic depyrimidination of dT yielding thymine and 2-deoxyribose (dRib).

168 almost exclusively directed incorporation of dT and dA.

169 This facilitates correct incorporation of dT via a Watson-Crick pair.

170 While insertion of dT can be catalyzed by polymerase eta, kappa, and iota,

171 ristic of complexes of KWK6 with this set of dT-mers, the distribution of binding free energies delta

172 ults also suggest that the high stability of dT-dA base pairs in the DNA provides a signal for the pa

173 Purification protocols using oligo dT's, locked nucleic acid substituted dT's, and tetramet

174 pic translocation rates of 3 nt s(-1) (oligo(dT)), 35 nt s(-1) (oligo(dU)), and 42 nt s(-1) (oligo(rU

175 titis C virus polymerase using poly(A)/oligo(dT(12)) that were or were not preannealed.

176 atures (T(m) values), however, poly(A)/oligo(dT(12-18)) is not expected to form stable duplexes.

177 nizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, terminates 3' oligo(rU) s

178 preannealing reactions for poly(A) and oligo(dT(12)), making it possible to characterize mechanism of

179 In this assay, a biotinylated oligo(dT(12)) primer was immobilized onto streptavidin-coated

180 it enzyme retains activity on poly(dA)/oligo(dT) templates but is impaired in its ability to extend s

181 ) lengths or in the elution profile of oligo(dT)-bound targets.

182 Analyses of the protein composition of oligo(dT)-selected UV photocross-linked ER protein-RNA adducts

183 ency of the thymidine oligonucleotide, oligo(dT)25, and providing a reliable surface for the amplific

184 either template RNA poly(A) or primer oligo(dT(12)) independently.

185 n vivo UV-crosslinking of RNA to RBPs, oligo(dT) capture and mass spectrometry yielded 1,145 differen

186 RNase H can even effectively replace oligo(dT)-based methods for standard RNA-seq.

187 supernatant are separated by a second oligo(dT) selection.

188 h precision and efficiency on a simple oligo(dT) tract, independent of other cis-elements or trans-fa

189 ing ITC, we find that DrSSB binding to oligo(dT)s with lengths close to the determined site size (50-

190 he isolation of polyadenylated RNA via oligo(dT), it will not provide RNA-binding information on prot

191 rotein-RNA complexes are purified with oligo(dT) magnetic beads.

192 An oligo-dT adapter incorporating a dUTP-containing PCR primer pr

193 First, an oligo-dT linked to an adaptor sequence is used to prime cDNA s

194 entify 3' ends, most techniques use an oligo-dT primer to construct deep sequencing libraries.

195 Polyadenylated mRNAs were captured by oligo-dT primers and processed into adapter-ligated cDNA libra

196 features flanking 3' ends derived from oligo-dT-based sequencing, we developed a naive Bayes classifi

197 better identify mis-priming events in oligo-dT primed sequences.

198 mRNA from genomic DNA using a modified oligo-dT bead capture and the respective whole-transcriptome a

199 The first using oligo-dT primers after polyadenylation of the bacterial RNA, t

200 ification, for the cDNA generated with oligo-dT and/or random oligonucleotide primers.

201 Ps were attached with 5'-NH(2)-tagged oligo-(dT)(25) primer and were used to isolate mRNA from breast

202 d (ss) dT(pdT)(absolute value(ZD) oligomers (dT-mers) where ZD = {-6, -10, -11, -14, -15} in the rang

203 e dimers in the all-thymine oligonucleotide (dT)(18) are fully formed in <1 ps after ultraviolet exci

204 Using a set of oligonucleotides (dT) with varying lengths as a molecular ruler and also a

205 binding, (SSB)(35) and (SSB)(65) formed on (dT)(70), with rates of interconversion on time scales th

206 he incorporation efficiency of dAMP opposite dT decreased 10(2)-10(3)-fold even when only one minor g

207 arameters for the insertion of dAMP opposite dT using primer/templates (P/T)-containing 3DA.

208 ex of hpol iota inserting N-MC-dATP opposite dT reveals that the adenine ring is stabilized in the an

209 ol iota-catalyzed insertion of dATP opposite dT.

210 propensity for GTP misincorporation opposite dT, predicting frequent A-->G errors in RNA with rates o

211 ion stage, with either an incorporated dA or dT opposite 1,N(6)-dA and 2'-deoxythymidine-5'-[(alpha,b

212 laminofluorene (FAAF), and N is either dA or dT.

213 nto template-primers containing either dC or dT residues 5' to the adduct, and the template-primers w

214 CG3*CNATC-3')(5'-GATNCGGCCGAG-3'), N = dC or dT] and -2 deletion [(5'-CTCG1G2CG3*CNATC-3')(5'-GATNGCC

215 G2CG3*CNATC-3')(5'-GATNGCCGAG-3'), N = dC or dT] duplexes, in which G* was either AF [N-(2'-deoxyguan

216 favorably with dC and dA but not with dG or dT.

217 complexes as well as heterodimeric peptide.[dT(10)C(10)T(10)] hairpin structures with triplex stems.

218 and drives the formation of ternary peptide.[dT(10)](2) complexes as well as heterodimeric peptide.[d

219 ntaining juxtaposed dC and 5'-phosphorylated dT deoxynucleotides (substrate 1) yielded kcat and kcat/

220 g this combined approach to poly dA and poly dT, we find that the global properties of these sequence

221 xperiments using streptavidin blocks or poly dT sequences located at either end of the ssDNA substrat

222 nwind double-stranded DNA that had a 3'-poly(dT) overhang as compared with double-stranded DNA with a

223 ared with double-stranded DNA with a 5'-poly(dT) or lacking a poly(dT) tail.

224 ded DNA with a 5'-poly(dT) or lacking a poly(dT) tail.

225 oligoriboadenylate is synthesized on a poly(dT) template by a recombinant form of the PRI1 protein a

226 g miRNA-DNA heteroduplexes, antipoly(A)-poly(dT) and anti-S9.6, were used.

227 dissociated into poly(dA).poly(dT) and poly(dT) increased dramatically (>12 degrees C) in the presen

228 imal to transcriptional start sites and poly(dT) tracts lying distal, and collectively define a symme

229 of ssDNA, which binds one tetramer, and poly(dT), which could bind several.

230 he occluded site size for scRPA binding poly(dT), as well as the stoichiometry, equilibrium binding c

231 T)(2) is enthalpy-driven and to poly(dA)poly(dT) is entropy-driven.

232 ively bind to the triplex DNA poly(dA)-[poly(dT)](2).

233 dA).2poly(dT) dissociated into poly(dA).poly(dT) and poly(dT) increased dramatically (>12 degrees C)

234 has a higher affinity for the poly(dA).poly(dT) DNA duplex than for the poly(dA).2poly(dT) DNA tripl

235 oly(dT) triplex but stabilizes poly(dA).poly(dT) duplex, suggesting the major groove as the binding s

236 -stranded DNA hairpin with 4-20-nt-long poly(dT) loops, with DeltaG(nWC) approximately -2.4 kcal/mol

237 22 genes were upregulated for LTA, LPS, Poly(dT), and Poly(I:C), and 12, 142, 249, and 16 genes were

238 genes were downregulated for LTA, LPS, Poly(dT), and Poly(I:C), respectively, with at least a 1-fold

239 s follows: 3.3+/-0.4 microM nucleotide (poly(dT)), 27+/-2 microM nucleotide (poly(dU)), and 36+/-2 mi

240 -Ag OBD crystallized in the presence of poly(dT)(12) is also reported.

241 lymer phosphorothioate oligonucleotide [Poly(dT)], and polyinosinic-polycytidylic acid [Poly(I:C)].

242 that the occluded site size of DrSSB on poly(dT) is approximately 45 nucleotides under low-salt condi

243 y(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface r

244 evisiae RNA using a surface coated with poly(dT) oligonucleotides to capture the RNAs at their natura

245 naturation studies with the poly(dA) x [poly(dT)]2 triplex structure, thioethers showed stabilization

246 bound preferentially to the poly(dA) x [poly(dT)]2 triplex, K(app) = 1.6 x 10(5) M(-1) (40 x K(app) f

247 V has the distinctive ability to ligate poly-dT single-stranded DNA and long dT overhangs in a Ku- an

248 the reverse transcription (RT) reaction-poly-dT priming, random priming and pooled exon-specific prim

249 gle internal mismatch is rC.dG > rG.dC >> rA.dT > rU.dA.

250 ngle internal mismatch is rG.dC > rC.dG > rA.dT > rU.dA.

251 n specific dynamic properties of the poly(rA/dT) segment and help advance our understanding of the me

252 latter results from rigidity of the poly(rA/dT) tract and leads to base-pair slippage of this sequen

253 reference and incompatibility of the poly(rA/dT) tract of the PPT with the nucleic acid conformation

254 ract region immediately 5' to the PPT, an rA:dT-rich sequence constituting the upstream portion of th

255 boxamide]-2'-deoxyuridine (Nap-dU) replacing dT.

256 contributions of rA.dA, rC.dC, rG.dG and rU.dT single internal mismatches were measured for 54 RNA/D

257 the single internal mismatches is rG.dG > rU.dT > rA.dA > rC.dC.

258 Measurements of the stoichiometry of scRPA-(dT)L complexes also show a [NaCl]-dependent change in st

259 ng constants, and binding enthalpy of scRPA-(dT)L complexes as a function of the oligodeoxynucleotide

260 ative cooperativity for binding of a second (dT)(35) molecule that is evident in Ec-SSB.

261 A similar dT-induced conformational heterogeneity was observed for

262 ptide KWK6 (ZL = +8) to single-stranded (ss) dT(pdT)(absolute value(ZD) oligomers (dT-mers) where ZD

263 re-formed 3' and/or 5' single-stranded (ss)-(dT)(n) flanking regions (tails) (n ranging from zero to

264 results are obtained for the single strand (dT)(20) by steady-state and time-resolved optical spectr

265 oligo dT's, locked nucleic acid substituted dT's, and tetramethylammonium chloride salts were charac

266 Decidual T (dT) cells but not peripheral T (pT) cells bound gal1 and

267 is nonlinear with a crossover from dsigma(T)/dT > 0 to dsigma(T)/dT approximately 0 as a function of

268 crossover from dsigma(T)/dT > 0 to dsigma(T)/dT approximately 0 as a function of the source-drain vol

269 rrent was the rate of change of temperature (dT/dt heat shock), not temperature itself.

270 t differ from those formed with a templating dT.

271 ntext, dA-rU base pairs are less stable than dT-rA base pairs.

272 The dT preference of the ligase is interesting given the seq

273 In the template DNA-DNA duplex, the dT-dA base pairs are more stable than the corresponding

274 calated and a carcinogen-exposed SMI for the dT/-2 duplex.

275 x dG-N2-TAM pair was higher than that of the dT x dG-N2-TAM pair in both sequences.

276 Irradiation of the dT-1 and dC-1 cross-linked products at 254 nm leads to a

277 d lethal replication stress after thymidine (dT)-induced inhibition of DNP dCTP synthesis by switchin

278 horylase (hTP) is responsible for thymidine (dT) homeostasis, and its action promotes angiogenesis.

279 horylase (hTP) is responsible for thymidine (dT) homeostasis, promotes angiogenesis, and is involved

280 polymerase eta bypass may lead to M(1)dG to dT and frameshift but likely not M(1)dG to dA mutations

281 ding to base substitutions (mostly M(1)dG to dT and M(1)dG to dA) and frameshift mutations.

282 mutational pattern is consistent with dG to dT transversions in the repetitive guanine tracts.

283 9), whereas 17 was hydrolyzed exclusively to dT.

284 t concentration and type for SSB binding to (dT)(70) under solution conditions that favor the fully w

285 ts of the deltaH(obsd) for scRPA binding to (dT)L at 1.5 M NaCl yield a contact site size of 28 nucle

286 initiate unwinding of blunt-ended and twin (dT)(6)-tailed DNA reflect processes needed to engage the

287 olution of the Pf-SSB tetramer bound to two (dT)(35) molecules.

288 Removal of the wobble hydrogen bonds in U:dT recovers a strong response to methylene substitution

289 ethylene substitution on the non-cognate UTP/dT scaffold ( approximately 3-fold decrease in kpol) is

290 tate for bond formation and cleavage for UTP/dT incorporation compared with ATP/dT incorporation.

291 roportion of -1 deletions were observed when dT was positioned 5' of M1dG.

292 Pharmacological co-targeting of the DNP with dT and the NSP with DI-39 was efficacious against ALL mo

293 A much higher reactivity was observed with dT than dC or dA.

294 the highest aptamer signal was obtained with dT 11mers, with shorter aptamer linkers significantly re

295 ghest hybridization signal was obtained with dT 5mer linkers, and the highest aptamer signal was obta

296 ing patterns of the guanine when paired with dT or dA and suggest that N7 alkylation may alter the ba

297 m of N7mdG involves in its base pairing with dT.

298 ientations to form Hoogsteen base pairs with dT.

299 e dissociation constant for labeled SSB(2) x dT(70) to be 1.1 microM at a high ionic strength (200 mM

300 ctures of the 20 kDa apo-YdbC dimer and YdbC:dT(19)G(1) complex were determined.