ライフサイエンスコーパス: dansyl

1 pten 5-dimethylamino-naphthalene-1-sulfonyl (dansyl).

2 nti-dextran) and one at the C terminus (anti-dansyl).

3 binding to and dissociation from the hapten dansyl.

4 ergy transfer from tryptophans in trypsin to dansyl.

5 onal determinants and by IgG-CD59 binding to dansyl.

6 show a reduced affinity for their target Ag, dansyl.

7 Dansyl cadaverine (N-dansyl-1,5-diaminopentane) was used to study the reactiv

8 the L chain variable region of a murine anti-dansyl Ab was joined to either human kappa or lambda con

9 study we have characterized a family of anti-dansyl Abs with the variable region of the heavy chain o

10 hexamer labeled with a naphthyl donor and a dansyl acceptor at the chain ends when the hexamer was s

11 fluorescence from bacterioopsin lacking the dansyl acceptor, nor did dansyl model compounds undergo

12 RET) as a probe, from tryptophan donors to a dansyl acceptor.

13 erent experiments performed with 850 nM 18:0-dansyl-ACP, binding to the second subunit of resting del

14 311-325 altered the fluorescence emission of dansyl-active site-labeled APC(i) but not a dansyl-activ

15 dansyl-active site-labeled APC(i) but not a dansyl-active site-labeled thrombin control, showing tha

16 measuring the isotope ratio of each isolated dansyl amino acid following hydrolysis and derivatizatio

17 500 nm with MelB-ST in the presence of 2'-(N-dansyl)aminoalkyl-1-thio-beta-D-galactopyranoside and Na

18 measurements with a fluorescent sugar (2'-(N-dansyl)aminoalkyl-1-thio-beta-D-galactopyranoside) demon

19 The product ion spectra of the dansyl and DMIS derivatives were dominated by ions repre

20 en more dramatic difference in depth between dansyl and mansyl (methylanilinonaphthalenesulfonyl) der

21 ionally, the Ab's specificity for the hapten dansyl and the convenient chemistry of dansyl provide a

22 sotropy measurements using acylated forms of dansyl- and fluoresceinyl-ACPs revealed equilibrium diss

23 This change in dansyl anisotropy was not observed when DEGR-Xa(i) was t

24 ate units were labeled with a naphthyl and a dansyl at the chain ends.

25 mbining site with specificity for the hapten dansyl, at the end of either C(H)1 or C(H)3 Ig regions.

26 ns were identified for the isomer containing dansyl attached to Gal 6', whereas only a single extende

27 Using fluorescence microscopy, a synthetic dansyl-avrainvillamide conjugate was observed to localiz

28 release profiles are characterized using 2,6-dansyl azide (2), an H2S specific fluorescent probe.

29 sequent click reactivity with nonfluorescent dansyl azide results in a 70-fold fluorescence increase.

30 imethylaminonaphthalene-5-sulfonyl chloride (dansyl-C1) at Lys75 (dansyl-calmodulin) were characteriz

31 Timed dansyl-C6-choline (DC6C) binding followed by its dissoci

32 Moreover, at low ionic strength, the dansyl-C6-choline affinities were similar in the absence

33 tics of the fluorescent acetylcholine analog dansyl-C6-choline at ionic strengths from 12.5 to 400 mM

34 Analysis of dansyl-C6-choline binding to the desensitized conformati

35 Fluorescent energy transfer measurements of dansyl-C6-choline binding to the nicotinic acetylcholine

36 Small cations did not compete directly for dansyl-C6-choline binding.

37 m fluorescent binding measurements show that dansyl-C6-choline binds with higher affinity to the alph

38 uorescence energy transfer measurements from dansyl-C6-choline bound to the agonist sites and also by

39 ined the affinity of the fluorescent agonist dansyl-C6-choline for the AChR.

40 escence kinetics showed that dissociation of dansyl-C6-choline from the AChR in the desensitized conf

41 ne (glutaminyl substrate) is cross-linked to dansyl cadaverine (amine substrate).

42 se cells, in the presence or absence of mono-dansyl cadaverine (MDC), a TG inhibitor.

43 Dansyl cadaverine (N-dansyl-1,5-diaminopentane) was used

44 caspase-3, in a manner suppressible by mono-dansyl cadaverine.

45 ited by the transglutaminase inhibitor, mono-dansyl cadaverine.

46 augmented by the transglutaminase inhibitors dansyl-cadaverine and cystamine, indicating that apoptos

47 on of N,N-dimethylated casein by Gly-OMe and dansyl-cadaverine suggest a complex kinetic mechanism fo

48 emonstrated that this peptide (694) bound to dansyl-calmodulin in a calcium-dependent fashion.

49 calmodulin, and had an apparent affinity for dansyl-calmodulin of 54 nM.

50 TA-calmodulin and Lys75-labeled dansyl-calmodulin thus distinguish between PDE and MLCK

51 The Km value for dansyl-calmodulin was equal to that of calmodulin, and t

52 ne-5-sulfonyl chloride (dansyl-C1) at Lys75 (dansyl-calmodulin) were characterized as activators of c

53 ges in the fluorescence emission spectrum of dansyl-calmodulin, and had an apparent affinity for dans

54 easured by CaM-Sepharose chromatography or a dansyl-CaM fluorescence assay.

55 BIAcore surface plasmon resonance (SPR) and dansyl-CaM fluorescence.

56 Fluorescence emission spectral analyses of dansyl-CaM showed apparent K(D) values of 65 +/- 30 nM f

57 fts in the fluorescence emission spectrum of dansyl-CaM, with apparent affinities of 87 +/- 23 nm and

58 Therefore, although dansyl can signal the binding event in both molecules, t

59 Comparing to the dansyl chloride (DnsCl) duplex isotope reagents, the new

60 homogenizer, followed by derivatization with dansyl chloride and analysis by liquid chromatography ta

61 ethod based on the derivatization of EE with dansyl chloride coupled with liquid chromatography/tande

62 veloped, using selective derivatization with dansyl chloride followed by HPLC with fluorescence detec

63 Dansyl chloride has been widely used as a derivatizing r

64 of the test compound cholesterol and excess dansyl chloride in CH(2)Cl(2) in the presence of 4-(dime

65 of directly derivatizing the polyamines with dansyl chloride on the ion-exchange resin.

66 solid-phase extractions and derivatized with dansyl chloride prior to reversed-phase LC/MS/MS.

67 phenols through derivatization with isotopic dansyl chloride reagents and on the analysis of the labe

68 ersely, the reaction of 5-cholesten-3-one or dansyl chloride with 2'-O-aminooxymethyl uridine provide

69 dimethylaminonaphthalene-1-sulfonylchloride (dansyl chloride) and chromatographed on a reversed-phase

70 In this study, we compared dansyl chloride, 1,2-dimethylimidazole-4-sulfonyl (DMIS)

71 uronan was combined with partially degraded, dansyl chloride-labeled aggrecan, blue fluorescent aggre

72 of surface water followed by derivation with dansyl chloride.

73 ivatization of estrogens for LC-ESI-MS/MS is dansyl chloride.

74 After pre-column derivatization with dansyl-chloride a Kinetex C18 core-shell particle column

75 etermined by LC-UV after derivatization with dansyl-chloride in both ground coffee and coffee beverag

76 beled products), resulting in a total of 315 dansyl compounds in the library.

77 was labeled active-site specifically with a dansyl (D) dye via a Glu-Gly-Arg (EGR) tether to yield D

78 shown to discriminate between enantiomers of dansyl-D,L-phenylalanine (DPs) by changing the local env

79 otein molecules, e.g., lysines modified with dansyl/dabsyl chloride and cystines/cysteines or phospho

80 labeled with either fluorescein (Fl-APC) or dansyl (DEGR-APC) donor, bound to phosphatidylcholine/ph

81 l photoaffinity reagents iodoacetamide 9 and dansyl derivative 10 were prepared.

82 UV-vis and fluorescence detection through a dansyl derivative.

83 ed and should be equally applicable to other dansyl-derivative amino acid enantiomers.

84 pared with a method based on the analysis of dansyl derivatives by LC and fluorescence detector (FD).

85 Therefore, the facile dansyl derivatization coupled with tandem mass spectral

86 The dansyl derivatization of EE introduced a basic secondary

87 A dansyl derivatized triazole linked glucopyranosyl conjug

88 Dansyl-derivatized, triazole-linked, glucopyranosyl conj

89 how the fluorescence emission properties of dansyl-DHPE can be exploited to characterize lipid extra

90 The ability of GM2AP to extract dansyl-DHPE from vesicles is altered by pH and the speci

91 and their membrane location, the behavior of dansyl (dimethylaminonaphthalenesulfonyl) and related po

92 aphthalene-1-sulfonyl)amino)undecanoic acid, dansyl-DL-alpha-aminocaprylic acid, and parinaric acid)

93 l properties of the CL-bound protein in four dansyl (Dns)-labeled variants of horse heart cyt c.

94 th high affinity for a convenient hapten Ag, dansyl (DNS; N,N-dimethyl-1-aminonaphthalene-5-sulfonyl

95 ose of the other fragment were tagged by the dansyl dye.

96 Fluorescence studies with dansyl-EGR-fIXa supported the high-affinity interaction

97 Fluorescence anisotropy [dansyl-EI-N(r)] and gel filtration of EI-N(r) show that

98 line increased the fluorescence intensity of dansyl emission excited at 280 nm by 10-40%.

99 substrates of the enzyme dansylcadaverine or dansyl-epsilon-aminocaproyl-Gln-Gln-Ile-Val.

100 te specificity, we examined the reactions of dansyl-epsilon-aminocaproyl-GlnGlnIleVal with three chem

101 but not G-actin quenched the fluorescence of dansyl ethylenediamine (DED) attached to Gin-41 by more

102 Cofilin binding enhanced the fluorescence of dansyl ethylenediamine (DED) attached to Gln41 on the DN

103 Actin labeled at Gln-41 with dansyl ethylenediamine (DED) via transglutaminase reacti

104 f residue 6 or 6' using galactose oxidase, a dansyl ethylenediamine acceptor fluorophore was coupled

105 The protein S-dependent dansyl fluorescence change was specific for fXa because

106 in the tenase assay and from the changes in dansyl fluorescence emission signal upon its binding to

107 anism was supported by an enhancement of the dansyl fluorescence of 5-(dimethylamino)-1-(naphthalenes

108 rescence studies showed that the recovery of dansyl fluorescence upon refolding occurred on a submill

109 s determined by time-resolved tryptophan and dansyl fluorescence.

110 scence energy-transfer (FET) kinetics from a dansyl fluorophore (Dns) introduced by derivatization of

111 esolved fluorescence anisotropy, utilizing a dansyl fluorophore attached to the DNA.

112 imer/template) oligonucleotides containing a dansyl fluorophore conjugated to a modified deoxyuridine

113 ar wires, D-4-Ad and D-8-Ad, consisting of a dansyl fluorophore linked to an adamantyl substrate anal

114 nce energy transfer between the naphthyl and dansyl fluorophore pair.

115 me of FXa labeled in its active sites with a dansyl fluorophore showed a similar concentration depend

116 bitor, and we labeled each cysteine with the dansyl fluorophore.

117 nd an intramolecular quencher of an attached dansyl fluorophore.

118 constraints and direct perturbations of the dansyl fluorophores placed the proteinase at the distal

119 Large direct effects on dansyl fluorophores were seen for only two positions in

120 his pattern was in the case of a dialkylated dansyl, for which two populations were observed.

121 Furthermore, Trp --> dansyl FRET measurements with a fluorescent sugar (2'-(N

122 Tryptophan to dansyl FRET was detected by measuring sensitized emissio

123 en plotted against varying concentrations of dansyl-GCIIL and an uncompetitive pattern against GGPP.

124 nt substrate inhibition by dansyl-GCIIL when dansyl-GCIIL was the varied substrate.

125 r GGPP, and K(M)(D) = 1.6 +/- 0.1 microM for dansyl-GCIIL were calculated from initial rates measured

126 P and induced potent substrate inhibition by dansyl-GCIIL when dansyl-GCIIL was the varied substrate.

127 ment that accompanies geranylgeranylation of dansyl-GCIIL.

128 this analogue also inhibits farnesylation of dansyl-GCKVL by FPP.

129 n effective substrate with a fourth peptide (dansyl-GCKVL).

130 GPP gave both analogue and farnesyl modified dansyl-GCVIM but only farnesylated dansyl-GCVLS.

131 de evidence that competitive modification of dansyl-GCVIM by ortho-trifluoromethoxy-AGPP stems from a

132 Competition for dansyl-GCVLS and dansyl-GCVIM peptides by FPP and ortho-trifluoromethoxy-

133 omethoxy-AGPP was efficiently transferred to dansyl-GCVIM.

134 To accomplish this, the pentapeptide N-dansyl-GCVLL was first enzymatically prenylated by human

135 ction, the single-turnover rate constant for dansyl-GCVLS alkylation was found to be the same for all

136 Competition for dansyl-GCVLS and dansyl-GCVIM peptides by FPP and ortho-

137 as iodo and cyano increased reactivity with dansyl-GCVLS and provided analogues that were effective

138 tho-substitutions on the aniline were potent dansyl-GCVLS modification FTase inhibitors (Ki in the 2.

139 Analysis of transfer kinetics to dansyl-GCVLS peptide revealed that AGPP analogues with s

140 3-6 are efficiently transferred to target N-dansyl-GCVLS peptide substrate by mammalian FTase.

141 ara-trifluoromethoxy-AGPP are transferred to dansyl-GCVLS while the ortho-substituted isomer was a po

142 modified dansyl-GCVIM but only farnesylated dansyl-GCVLS.

143 lowing a previous report suggesting that 6-O-dansyl GlcNAc is biosynthetically incorporated into chit

144 active 5-dimethylaminonaphthalene-1sulfonyl (dansyl) Glu-Gly-Arg-(DEGR)-chloromethylketone-factor Xa

145 each 5-dimethylaminonaphthalene-1-sulfonyl (dansyl)-Glu-Gly-Arg-IXa (dEGR-IXa) with the A2 subunit w

146 and the peptide quenched the fluorescence of dansyl-Glu-Gly-Arg active site-modified factor Xa, sugge

147 of substrate or to the active site inhibitor dansyl-Glu-Gly-Arg-chloromethyl ketone (DEGR-CMK) in a s

148 Active-site labeling with dansyl-glutamyl-glycyl-arginyl chloromethyl ketone or im

149 mine moiety serves as electron donor and the dansyl group acts as the acceptor.

150 was slightly affected by the presence of the dansyl group at the N-terminus.

151 This shows the dansyl group has a strong tendency to seek a shallow loc

152 Even more strikingly, the dansyl group in Dns-Gly-His-Gly (3) exhibited a profound

153 e calix[4]arenebis(crown-6) (1) containing a dansyl group in the proton-ionizable side arm has been e

154 h copper, while in Dpr(Dns)-His-Lys (2), the dansyl group is also on a side branch but the close plac

155 In Gly-His-Lys(Dns) (1), the dansyl group is on a side branch and does not interact w

156 energy transfer (FRET) from tryptophan to a dansyl group on Lys 41.

157 -substituted azacrown ethers attached with a dansyl group, in which the p-phenylenediamine moiety ser

158 naphthyl sulfonic acid (AANS), acrylodan, or dansyl groups as fluorophores.

159 ansfer from the introduced tryptophan to the dansyl groups decreased dramatically upon unfolding.

160 The rate of thiol-disulfide exchange of dansyl groups mediated by dithiothreitol depends on the

161 To further probe this partial ordering, dansyl groups were introduced via cysteine residues at t

162 Dantyl groups were located deeper than dansyl groups, 13-16 A from the bilayer center.

163 pentamers were end-labeled with naphthyl and dansyl groups.

164 Quenching was measured for dansyl groups: (1) attached to the polar headgroup of PE

165 mino)-1-naphthalenesulfonyl-Gly-Ser-Arg-NH2 (dansyl-GSR-NH2) has emerged as one of the compounds in t

166 ing GM2, we find that GM2AP readily extracts dansyl-headgroup-labeled lipids as well as other phospho

167 -NP (4-hydroxy-3-nitrophenylacetyl) and anti-dansyl IgE-mediated passive cutaneous anaphylaxis in tra

168 passive cutaneous anaphylaxis; and attenuate dansyl IgE-mediated systemic anaphylaxis in human Fcepsi

169 2)(mu-O(2)CR)(4)(L)(2)], with R = Me and L = dansyl-imidazole (Ds-im) or dansyl-piperazine (Ds-pip).

170 To see if the polar sulfonamide group of dansyl influences depth, a structurally related probe su

171 ics of the binding of a fluorescent peptide (dansyl-KLIGVLSSLFRPK, fVSV13) to the Escherichia coli mo

172 casein (NMC) and N-Boc-Lys-NH-CH(2)-CH(2)-NH-dansyl (KXD), respectively.

173 two different fluorescent amino acids (beta-dansyl-L-alpha,beta-diaminopropionic acid (dnsDap4) or b

174 Concentration changes in the FIA system of dansyl-L-arginine over the range of 0.38-3.04 mM are als

175 rombin preparation methods and on the use of dansyl-l-arginine-(3-methyl-1,5-pantanediyl)amide and a

176 hat MS-325 can displace dansyl sarcosine and dansyl-L-asparagine from HSA with inhibition constants (

177 addition of a 1.52 mM solution of N epsilon-dansyl-L-lysine, the sample duration and time of analyte

178 5-dimethylamino-1-naphthalenesulfonamide and dansyl-l-proline from the two major drug-binding sites o

179 ddition, in the analysis of a (13)C2-/(12)C2-dansyl labeled human urine, IsoMS detected 2044 peak pai

180 e/phenol submetabolome from the (12)C-/(13)C-dansyl labeled lysates of 100, 1000, and 10000 cells, re

181 ble for data processing, five (13)C2-/(12)C2-dansyl labeled metabolite standards were analyzed by LC-

182 Because dansyl labeled metabolites can be captured on a reversed

183 mpoundID.org for automated identification of dansyl labeled metabolites in a sample based on matching

184 peak pairs could be either identified using dansyl labeled standard library or mass-matched to chemi

185 A calibration curve of a mixture of 17 dansyl-labeled amino acid standards is used to determine

186 5, and the ability of these peptides to bind dansyl-labeled calmodulin and the calcium dependence of

187 However, when targeted to a dansyl-labeled cell membrane, C(H)1-DAF was significantl

188 59 fusion proteins all bound specifically to dansyl-labeled Chinese hamster ovary cells and provided

189 Using fluorescence anisotropy of dansyl-labeled GroES, intrinsic fluorescence, bis-ANS bi

190 dan, Laurdan, pyrene-labeled fatty acid, and dansyl-labeled phospholipid) were used to assess changes

191 ce anisotropy measurements were performed on dansyl-labeled primer-templates bound to the mutant enzy

192 escence anisotropy decay measurements with a dansyl-labeled primer/template indicate that the Q419A,

193 alkyl linker, the fluorescence intensity of dansyl-labeled S-nitrosothiols could be enhanced up to 3

194 In addition, one of the dansyl-labeled S-nitrosothiols was used to explore the k

195 Mixing StAR with dansyl-labeled vesicles composed of phosphatidylcholine

196 UMS of human urine was prepared by (13)C2-dansyl labeling of a pooled sample from 20 healthy indiv

197 As an example, (12)C-/(13)C-dansyl labeling of the metabolites in lysates of 100, 10

198 A self-aggregated 48-membered dansyl library was screened against a series of metaboli

199 These analogues incorporate anthranylate or dansyl-like groups anchored to the terpenoid skeleton th

200 bimolecular (noncooperative) process between dansyl-linked disulfides and dithiothreitol.

201 ioopsin lacking the dansyl acceptor, nor did dansyl model compounds undergo a similar transition.

202 cle-bound DEGR-fXa(i), the anisotropy of the dansyl moiety was altered from 0.219 +/- 0.002 to 0.245

203 xible linker that incorporates a fluorescent dansyl moiety was synthesized and used to connect two hi

204 acid fragment was labeled at the 3'-end by a dansyl molecule prone to be included into the macrocycle

205 r a low molecular weight substrate, N(alpha)-dansyl-N(omega)-(1,N(6)-etheno-ADP-ribosyl)-arginine met

206 as demonstrated by fluorescence titration of dansyl-ND8 with G-actin.

207 pproximately 64 A were estimated between the dansyl on DEGR-APC and rhodamine in PC/PS/GlcCer(OR) and

208 ligodeoxynucleotides were tagged with either dansyl or eosin fluorophores at their 5' termini and ann

209 lycerol (DAG), determined from tryptophan to dansyl-PE resonance energy transfer (RET) measurements,

210 lfonyl]dipalmitoylphosphatidyleth anolamine (dansyl-PE) decreased, indicative of an increased water c

211 The interactions of GroEL with six dansyl peptides were investigated by means of our previo

212 The labeling procedure with dansyl-PGGQQIV probe revealed that Lys157, Lys503, Lys62

213 ine (dansylcadaverine), [14C]putrescine, and dansyl-PGGQQIV.

214 eaved 5-dimethylaminonaphthalene-1-sulfonyl (dansyl)-Phe-Ala-Arg with a pH optimum of 5.5-6.5.

215 eaves 5-dimethylaminonaphthalene-1-sulfonyl (dansyl)-Phe-Ala-Arg, although the level of enzyme activi

216 ing that the third domain is inactive toward dansyl-Phe-Ala-Arg.

217 e activity (<0.004% of wild-type CPE) toward dansyl-Phe-Ala-Arg.

218 Dansyl-Pro-Ala-Arg and dansyl-Phe-Phe-Arg were cleaved with Km values of 17 and

219 us StAR tryptophan residues as the donor and dansyl-phosphatidylethanolamine in the bilayer as the ac

220 h R = Me and L = dansyl-imidazole (Ds-im) or dansyl-piperazine (Ds-pip).

221 nd even with a moderate increase compared to dansyl-PQR-NH2 in its predicted ability to penetrate the

222 ith 5-(dimethylamino)-1-naphthalenesulfonyl (dansyl-PQR-NH2), antagonizes the central anti-opioid act

223 Dansyl-Pro-Ala-Arg and dansyl-Phe-Phe-Arg were cleaved w

224 actant were utilized to attach a fluorescent dansyl probe and/or atom-transfer radical polymerization

225 A dansyl probe attached to the DNA was used to measure the

226 esolved fluorescence anisotropy decay of the dansyl probe was analyzed in terms of two local environm

227 In all cases, the dansyl probes located in the polar headgroup region, 19-

228 The dansyl probes were used to determine proximity of the pr

229 apten dansyl and the convenient chemistry of dansyl provide a means to link IL-2 to virtually any mol

230 alene-1-sulfonyl)dioleoylphosphatidylserine (dansyl-PS), which probes the lipid environment of PKC, a

231 e that was modified to include an N-terminal dansyl reporter group.

232 the "overlap" between the SNO moiety and the dansyl ring is favored because of their stabilizing inte

233 cement studies show that MS-325 can displace dansyl sarcosine and dansyl-L-asparagine from HSA with i

234 c degranulation; suppress peanut-, cat-, and dansyl-specific IgE-mediated passive cutaneous anaphylax

235 extended these studies by producing the same dansyl-specific IgG1 in cell lines deficient in attachme

236 several N-sulfonyl-polyamines, including N1-dansyl-spermine (N1-DnsSpm) and N1-(n-octanesulfonyl)-sp

237 ed by mass and retention time matches to the dansyl standard library and 2988 pairs with their masses

238 Using a dansyl standard library, we were able to identify 83 met

239 ziridine and a fluorescent alkylating agent, dansyl sulfonate aziridine, inactivated three different

240 scence resonance energy transfer between 2,6-dansyl tethered to the active site of Xa and eosin tethe

241 Dansyl-to-heme distance distributions [P(r)] during fold

242 ive sites of vimentin and, by sequencing the dansyl-tracer-labeled segments of the protein, we have s

243 mol of zinc/mol of enzyme for maximal (S)-N-dansyl-Tyr-Val-alpha-hydroxyglycine dealkylation activit

244 i-dextran V(H) CDR2 replaced CDR2 of an anti-dansyl V(H), the glycosylation site was used, but H chai

245 Cleavage of dansyl-VIP by tryptase was completely inhibited by DesPr

246 nded population (19.7 A) was determined when dansyl was attached to Gal 6.

247 n complex formation, the microenvironment of dansyl was modified in such a way that the fluorescent i

248 rgy transfer (FRET) from the naphthyl to the dansyl was observed in 2:1 hexane/ethyl acetate (EA) con

249 which have an extra phenyl group relative to dansyl, were found to locate deeply within the acyl chai

250 From titrations of MyoD-dansyl with E12 at 100 mM KCl, a free energy of heterodi

251 The titrations of E12-dansyl with MyoD yielded a free energy of 8.3 kcal/mol w

252 hen the oxidized enzyme was reacted with the dansyl-YVG substrate and H(2)O(2), the alpha-hydroxyglyc

253 ence of the PHM-catalyzed monooxygenation of dansyl-YVG was studied in two different buffer systems i

254 s found to be dominated by k(cat), with K(m)(dansyl-YVG) remaining pH-independent over the pH range o