ライフサイエンスコーパス: deacylate

1 tions can occur only when the P-site tRNA is deacylated.

2 post-transfer editing a misacylated tRNA is deacylated.

3 PLAP was acylated and then progressively was deacylated.

4 bated with increasing concentrations of NaOH-deacylated 1291 LOS.

5 ed [3H]LPS, animals expressing the transgene deacylated a larger fraction of the [3H]LPS taken up by

6 as an independent protein, it was capable of deacylating a mischarged Ala-microhelixPro variant.

7 action known as pretransfer editing, and (3) deacylating a mischarged Ala-tRNA(Pro) variant via a pos

8 This enzyme also is capable of rapidly deacylating a mischarged Ala-tRNA(Pro) variant.

9 Providing recombinant AOAH restored LPS deacylating ability to Aoah(-/-) mice and prevented LPS-

10 aining purified LPS-binding protein, the LPS-deacylating activity of MNC greatly exceeded that of PMN

11 o the prokaryotic INS domain, was capable of deacylating Ala-tRNAPro and Ala-microhelixPro variants b

12 e ProRS editing domain, is capable of weakly deacylating Ala-tRNAPro.

13 n GC and lactosylceramide (LacCer) and their deacylated analogues.

14 ng the association and dissociation rates of deacylated and aminoacylated tRNAs to the A-site and P-s

15 that acylate the enzyme but are only slowly deacylated and can therefore act also as potent inhibito

16 Deacylated lipid A, deacylated and palmitoylated lipid A, palmitoylated lipi

17 treatment of a ribosomal complex containing deacylated and peptidyl-tRNAs bound in the A/P and P/E s

18 AOAH) is an eukaryotic lipase that partially deacylates and detoxifies Gram-negative bacterial lipopo

19 ressed by antigen (Ag)-presenting cells that deacylates and thereby inactivates LPS in host tissues.

20 oxysuccinimidyl carbamate, ester lipids were deacylated, and the reaction mixtures were subjected to

21 formed by HPLC-PDA-ESI-MS(n) and extended to deacylated anthocyanins and aglycones, obtained, respect

22 The 95-kDa protein also deacylated arachidonoyl groups from 1-O-hexadecyl-2-arac

23 y SDS-polyacrylamide gel electrophoresis and deacylated arachidonoyl-lysophosphatidylcholine (ara-lys

24 and 5-methylcytidine is rapidly degraded and deacylated at 37 degrees C in a trm8-Delta trm4-Delta st

25 bstituted lysoPC, since palmitoyl-lysoPC was deacylated at a much lower rate (7 micromol/(min mg)).

26 by acyloxyacyl hydrolase, a host enzyme that deacylates bacterial lipopolysaccharides.

27 RNALys, and Met-tRNALys were essentially not deacylated by LysRS, indicating that the enzyme does not

28 tructurally elucidated, and then shown to be deacylated by recombinant Tem25.

29 Ceramidases deacylate ceramides, important intermediates in the meta

30 CmaE will only recognize deacylated CmaA for initial complexation.

31 Under identical settings, deacylated cyanin, ferulic and sinapic acids had kinh of

32 In contrast, the INS domain is unable to deacylate Cys-tRNA(Pro), which is hydrolyzed exclusively

33 A or DNA containing dG-C8-AAF or the related deacylated dG-C8-AF adduct.

34 Unlike deacylated elongator tRNAs, N-acetyl-aminoacyl-tRNAs and

35 In this study, we demonstrate that ceramide-deacylating enzyme acid ceramidase (AC) was preferential

36 OS generated by treatment of wt LOS with the deacylating enzyme, acyloxyacylhydrolase.

37 s that lacked acyloxyacyl hydrolase, the LPS-deacylating enzyme, prolonged drainage of fully acylated

38 the C7 hydroxyl of baccatin III must remain deacylated for enzyme function.

39 on is accelerated when the protein is in its deacylated form (dSP-C).

40 y of conversion from the hexaacyl to the 3-O-deacylated form by heterologous lipid A 3-O-deacylase (P

41 ophosphocholine and is specific for the sn-2-deacylated form of plasmalogen.

42 ines is caused by a rapid degradation of the deacylated form of the abnormal mt-tRNA(Val).

43 the conversion of acylated peptides to their deacylated forms.

44 zed an enzyme acyl-protein thioesterase that deacylates Galpha proteins and at least some other thioa

45 mechanical and physicochemical properties of deacylated gellan matrices is presented.

46 iesterase (GDPD) catalyzes the hydrolysis of deacylated glycerophospholipids to glycerol phosphate an

47 They also fail to deacylate Gpa1p, the yeast G alpha subunit, in metabolic

48 M1 protein, while the stunting of fusion by deacylated HA acting in isolation may be balanced by oth

49 Compared to wild-type HA, deacylated HA is correlated with released particles with

50 ed cardiolipin progressing to the completely deacylated headgroup.

51 The role of 3-O-deacylated lipid A among nitrogen-fixing endosymbionts,

52 in production of significant amounts of 3'-O-deacylated lipid A in growing cultures.

53 bility to add aminoarabinose to lipid A, 3-O-deacylated lipid A species were not detected, despite th

54 Finally, deacylated lipid A species were not observed in some cli

55 lso contains a significant proportion of 3-O-deacylated lipid A species.

56 Deacylated lipid A, deacylated and palmitoylated lipid A

57 y, we demonstrated that the mutant lacks 3-O-deacylated lipid A.

58 Further analysis of internally radiolabeled deacylated lipids from the SmT morphotype, by high-perfo

59 Mass spectrometry analysis of the O-deacylated LOS of the R127K point mutation confirmed the

60 Analysis of HF-treated and O-deacylated LOS revealed three major components present i

61 AH treatment of LOS-sCD14 produced partially deacylated LOS still complexed with sCD14.

62 When the O-deacylated LOS were analyzed by mass spectrometry, both

63 AOAH-deficient mice did not deacylate LPS and, whereas their inflammatory responses

64 the cells' ability to kill Escherichia coli, deacylate LPS, and degrade bacterial protein.

65 AOAH and secrete it into urine, where it can deacylate LPS.

66 H can then be used by the recipient cells to deacylate LPS.

67 n this current study, we have analyzed the O-deacylated LPS and free oligosaccharides from three tran

68 Enzymatically deacylated LPS is much less potent than LPS at inducing

69 Transgenic mice deacylated LPS more rapidly than did wild-type controls.

70 ponsive; and 2) substoichiometric amounts of deacylated LPS that block LPS signaling at a site distal

71 The immunomodulatory properties of PagL-deacylated LPS were compared with another pentaacyl form

72 h only one acyl chain (LTA-1) and completely deacylated LTA (LTA-0).

73 -tRNA synthetase is unable to hydrolytically deacylate misacylated tRNA(Val) terminating in 3'-pyrimi

74 (Val) terminating in 3'-pyrimidines but does deacylate mischarged tRNA(Val) terminating in adenosine

75 ited earlier studies demonstrated that AlaXp deacylated mischarged tRNA(Ala) in vitro, the significan

76 Ss possess an amino acid editing domain that deacylates mischarged Ala-tRNAPro, editing of Cys-tRNAPr

77 iposomes with either a low or high dose of 3-deacylated monophosphoryl lipid A (MPL) and administered

78 glycoprotein D from HSV-2 with alum and 3-O-deacylated monophosphoryl lipid A as an adjuvant; contro

79 oprotein-D-subunit vaccine with alum and 3-O-deacylated-monophosphoryl lipid A in subjects whose regu

80 t charged with the starved amino acid became deacylated more rapidly than tRNAs charged with the star

81 ectrospray ionization mass spectrometry of O-deacylated MS11mkC LOS produced ions consistent with kno

82 ylation interrupted HA-M1 interactions since deacylated mutant HA failed to incorporate an M1 layer w

83 lso exhibits an acylase activity, capable of deacylating N-acetyl-Met-Leu-p-nitroanilide and N-triflu

84 In contrast, ValRS can deacylate non-cognate amino acids from the 2'-OH.

85 RRF flexibility plays a role in removing the deacylated P-site tRNA during termination of translation

86 The deacylated P-site tRNA has moved into a partly transloca

87 ents increases the synthesis of enzymes that deacylate, palmitoylate, hydroxylate, and attach aminoar

88 and 6-fold above those of the commercial and deacylated PEI25s, respectively; moreover, PEI87 deliver

89 ation of the potential of such linear, fully deacylated PEIs in gene therapy for lung diseases, syste

90 In other bacteria, GlpQ hydrolyzes deacylated phospholipids to glycerol-3-phosphate (G3P) w

91 G3P exist among borreliae: (i) hydrolysis of deacylated phospholipids, (ii) reduction of DHAP, and (i

92 vestigated purified and, more importantly, O-deacylated pnLTA, which is most suitable for NMR spectro

93 The deacylated products of the soluble derivatives of phosph

94 ,4-P2, as determined by HPLC analysis of the deacylated products.

95 ther, these abnormalities suggest that SIRT5 deacylates protein substrates involved in cellular oxida

96 ted with 1 M hydroxylamine which is known to deacylate proteins.

97 oxyacyl hydrolase, the leukocyte enzyme that deacylates purified LPS, to attack LPS residing in the b

98 APT1) was identified as an enzyme capable of deacylating some thioacylated proteins in vitro.

99 Deacylated SP-C (dSP-C), unchanged in composition and se

100 ic G proteins cycle between thioacylated and deacylated states in a receptor-regulated fashion.

101 K(m) values of 0.42 mM and 0.40 mM for the N-deacylated taxoid and benzoyl-CoA, respectively.

102 ntermediate and subsequently employ water to deacylate the beta-lactam and release product.

103 rty of dendritic cells (DCs), the ability to deacylate the lipid A moiety of gram-negative bacterial

104 NA synthetases have an editing activity that deacylates the mischarged amino acid before capture by t

105 Phospholipids are extracted and deacylated to glycero-head groups, which are then separa

106 diolipin (CL) that is synthesized de novo is deacylated to monolysocardiolipin (MLCL), which is reacy

107 almitoylglycerophosphocholine, which is then deacylated to provide substrates for chain elongation an

108 During protein synthesis, deacylated transfer RNAs leave the ribosome via an exit

109 therefore be recycled by releasing mRNA and deacylated tRNA and by dissociating ribosomes into subun

110 igatin and MCT-1/DENR can promote release of deacylated tRNA and mRNA from recycled 40S subunits afte

111 the termination step of protein synthesis, a deacylated tRNA and mRNA remain associated with the ribo

112 e post-peptidyl transferase translocation of deacylated tRNA and peptidyl tRNA to the ribosomal E- an

113 at the rate-limiting step is dissociation of deacylated tRNA and/or amino acid product and highlights

114 tRNA binds in the classical P site, whereas deacylated tRNA binds mostly in an intermediate P/E posi

115 k(on)) of tRNA dramatically, suggesting that deacylated tRNA binds the P site of the ribosome via the

116 acilitates binding, movement, and release of deacylated tRNA by remodeling the structure of the 50S s

117 unit E site that interacts with the elbow of deacylated tRNA during protein synthesis.

118 le movements coupled to the translocation of deacylated tRNA during protein synthesis.

119 s been proposed to facilitate the removal of deacylated tRNA from the E-site.

120 ed by two tRNA molecules and that release of deacylated tRNA from the exit (E) site is uncoupled from

121 binding of EF-G would trigger the removal of deacylated tRNA from the P site by moving RRF toward the

122 and is translocated to the P-site, releasing deacylated tRNA from the P- and E-sites.

123 ribosomal subunits, and release of mRNA and deacylated tRNA from the PoTC, are unclear.

124 , a function performed by eEF1A, by removing deacylated tRNA from the ribosomal Exit site.

125 ation ribosomes containing peptidyl-tRNA and deacylated tRNA in the classical P/P and E/E states, res

126 te constants reveals that destabilization of deacylated tRNA in the E-site, rearrangement of peptidyl

127 Movement of the acceptor stem of deacylated tRNA into the 50S E site and EF-G binding to

128 state of binding that precedes the entry of deacylated tRNA into the F (final) site from which it di

129 between the ribosomal L1 stalk and the newly deacylated tRNA is established spontaneously upon peptid

130 is at initiation in response to increases in deacylated tRNA levels in the cell.

131 Deacylated tRNA may be a factor negatively regulating p7

132 ter two cycles of elongation, when the first deacylated tRNA reached the E-site after translocation f

133 r translational termination, mRNA and P site deacylated tRNA remain associated with ribosomes in post

134 regulatory role of the exit (E) site, where deacylated tRNA spontaneously dissociates from the trans

135 We show that the 3'-end of the deacylated tRNA that is formed after transpeptidation do

136 This reduction in deacylated tRNA was accompanied by decreased synthesis o

137 ad domain and contact of the acceptor arm of deacylated tRNA with helix 68 of 23S rRNA.

138 the absence of editing lowered the amount of deacylated tRNA(Phe) in the cell.

139 ibosomes remain associated with mRNA, P-site deacylated tRNA, and release factor eRF1 and must be rec

140 PoTC), composed of the ribosome, mRNA, and a deacylated tRNA, is processed by the concerted action of

141 h is activated by amino acid deprivation via deacylated tRNA, was found to be active in rodent brain,

142 Gcn2p is activated by deacylated tRNA, which accumulates when tRNA aminoacylat

143 y the eIF2alpha kinase, GCN2, in an apparent deacylated tRNA-independent fashion.

144 tivity of elongation factor-2 and removal of deacylated tRNA.

145 lipid-independent trans editing factor that deacylates tRNA.

146 usly unidentified hybrid state in which only deacylated-tRNA adopts its hybrid (P/E) configuration.

147 c ATPase proposed to catalyze the release of deacylated-tRNA from the ribosomal E-site.

148 Aminoacyl-tRNA, peptidyl-tRNA, and deacylated-tRNA were bound in various combinations to th

149 The position of deacylated tRNAfMet depends on the buffer condition used

150 Either deacylated tRNAfMet or fMet-tRNAfMet were bound to the 7

151 ed accumulation of Tyr-tRNAPhe (5%), but not deacylated tRNAPhe during amino acid starvation, limitin

152 When mRNA and deacylated tRNAs are bound to the 70S ribosome and EF-G-

153 nthetase (HisRS) postulated to bind multiple deacylated tRNAs as a general sensor of starvation.

154 inhibit protein synthesis by competing with deacylated tRNAs for E site binding.

155 s region is involved in the translocation of deacylated tRNAs from the P to the E site.

156 onditions favoring movement of the resulting deacylated tRNAs into the P/E hybrid state, leads to sim

157 Pairing of amino acids and deacylated tRNAs is catalyzed by aminoacyl-tRNA syntheta

158 cordance with this model, GCN2 bound several deacylated tRNAs with similar affinities, and aminoacyla

159 se (IleRS) and valyl-tRNA synthetase (ValRS) deacylate Val-tRNA(Ile) and Thr-tRNA(Val), respectively.

160 s A beta-lactamases, it is proposed that the deacylating water molecule is deactivated by interaction

161 Specifically, Asn170 that coordinates the deacylating water molecule is misaligned, in both the fr

162 so as to block the approach of a potentially deacylating water molecule.

163 roup interacts with Asn132, but not with the deacylating water molecule.

164 ation and prevent their interaction with the deacylating water molecule.

165 e binding groove) to the active site and the deacylating water.

166 K84, with implications for activation of the deacylating water.

167 24S) is a relatively unreactive acyl enzyme, deacylating with a pKa of 7.1 and a rate constant of 0.0