ライフサイエンスコーパス: deacylation

1 n, aminoacylation, and posttransfer editing (deacylation).

2 nt for thiolation (acylation) or hydrolysis (deacylation).

3 conserved aspartic acid abolished tRNA(Leu) deacylation.

4 marins were efficiently produced without any deacylation.

5 to activate the hydrolytic water molecule in deacylation.

6 ns accelerate leaving group dissociation and deacylation.

7 ions have been shown to increase the rate of deacylation.

8 a stable tyrosinate as the general base for deacylation.

9 cing the E166A mutation that greatly retards deacylation.

10 er are all associated with the first step of deacylation.

11 be exposed to hydrolyzing water and aqueous deacylation.

12 a ypk1Delta mutant curtails the increased PC deacylation.

13 ge in the rate-limiting transition state for deacylation.

14 ted by acylation with some contribution from deacylation.

15 This substitution also had no effect on deacylation.

16 with almost no apparent effect on binding or deacylation.

17 cus aureus results in a protein incapable of deacylation.

18 n characteristic of His-57-catalyzed complex deacylation.

19 distort the proteinase active site and slow deacylation.

20 conformational change and the rate of enzyme deacylation.

21 ed-pathway kinetic scheme, and rate-limiting deacylation.

22 duced rapid and extensive CD14-dependent LPS deacylation.

23 ociation affinity, but not to a fast rate of deacylation.

24 ot released from the membrane after chemical deacylation.

25 adenosinediphospho(ADP)-ribosylation, and/or deacylation.

26 a-diketones followed by an unexpected tandem deacylation.

27 e on-rate for acylation and the off-rate for deacylation.

28 ivities of key metabolic enzymes via protein deacylation.

29 hat were attributable at least in part to PC deacylation.

30 olecule that likely is involved in substrate deacylation.

31 bstrate, reflecting both rapid acylation and deacylation.

32 by substrate but is impaired with respect to deacylation.

33 highlight its specific role in acylation and deacylation.

34 at coordinate a hydrolytic water involved in deacylation.

35 he hydrolytic water and allows for efficient deacylation.

36 Fluoride effects on cell deacylation ([3]H-C20:4 release) and PLA2 activity were

37 Indeed, during Zemplen deacylation, a chloroacetamide chlorine atom was displac

38 was shown to require both aminoacylation and deacylation activities.

39 binding to K70, which in turn correlates to deacylation activity.

40 t that, besides phosphorylation, S-acylation/deacylation also regulates SLN activity.

41 ophil elastase (HNE) showed similar rates of deacylation and enhanced susceptibility to proteolysis b

42 hat a ypk1Delta mutant exhibits increased PC deacylation and glycerophosphocholine production compare

43 ectrum compared with that of the LOS after O-deacylation and hydrogen fluoride treatment.

44 We now demonstrate that 3-O-deacylation and palmitoylation of lipid A decreases its

45 te from Ha-Ras, implying that SNC stimulated deacylation and permitted subsequent reacylation of Ha-R

46 32 to orient bound carbapenems for efficient deacylation and prevent their interaction with the deacy

47 Es and gammaKA-PEs using CH(3)NH(2)-mediated deacylation and quantitation of the resulting glyceropho

48 remodeled through the Lands cycle, i.e. the deacylation and reacylation of PC to attain the final an

49 The cycle of deacylation and reacylation of phospholipids plays a cri

50 o evidence for His-57 involvement in complex deacylation and was instead characteristic of a hydroxid

51 th initiator caspases 8 or 1, (ii) very slow deacylation, and (iii) loss of the caspase p10 subunit f

52 A2-VIA as an important enzyme in cardiolipin deacylation, and as a potential target for therapeutic i

53 ly proteases but also enzymes that perform N-deacylation, and enzymes that catalyze N-desuccinylation

54 he deletion of the omega-loop eliminates the deacylation apparatus comprising Glu166 and its associat

55 site of acylation or the nucleophile site of deacylation, appears to be hydrogen-bonded to the hydrox

56 sis involves inositol acylation and inositol deacylation as discrete steps at the beginning and end o

57 acetate into fatty acids, we quantitated LPS deacylation as the loss of radiolabeled secondary (laura

58 d by k(3)) or that neither the acylation nor deacylation became the sole rate-limiting step.

59 d radiolabeled LPS underwent inactivation by deacylation before it left the footpad; in animals that

60 ial and selective acyloxyacyl hydrolase-like deacylation, both after phagocytosis of intact bacteria

61 cyl group has little effect on the rate of N-deacylation but increases the N/O selectivity ratio.

62 -exchange LTA and loses 500 mass units after deacylation by alkali hydrolysis.

63 Deacylation by AOAH greatly reduces the ability of LPS t

64 d differences in the efficiency of endotoxin deacylation by AOAH were observed, with the following ra

65 rrier higher than that for the first step of deacylation by approximately 4 kcal/mol.

66 Deacylation by K70A, but not K70D or K70E, can be partia

67 Deacylation by NAD(+)-dependent sirtuin reactions yields

68 order of magnitude, but affects the rate of deacylation by over 5 orders of magnitude.

69 ng group P must dissociate before hydrolytic deacylation can occur.

70 by which human sirtuins function as protein deacylation catalysts.

71 l, we propose a substrate-assisted acylation/deacylation-catalytic mechanism in which the amino group

72 acids such as arachidonic acid requires sn-2-deacylation catalyzed by a class of enzymes known as pho

73 cules acquire palmitic acid via an acylation/deacylation cycle and that this profile changes during d

74 We also show that an acylation-deacylation cycle is important for the steady-state loca

75 S-acylation/deacylation cycles and vesicular transport are critical

76 of the ligand-free mutant enzyme and of the deacylation-defective wild-type and mutant cephalexin ac

77 compared to those of wild-type (WT) or even deacylation deficient forms of the enzyme.

78 trapped as a trans-enamine intermediate in a deacylation deficient SHV variant, we designed a novel p

79 y that the reaction between tazobactam and a deacylation deficient variant of SHV-1 beta-lactamase, E

80 By using the E166A deacylation deficient variant of the enzyme, we were abl

81 pretation of experimental data obtained with deacylation-deficient beta-lactamases to make mechanisti

82 s sulbactam and clavulanic acid bound to the deacylation-deficient E166A variant of SHV-1 beta-lactam

83 data and computer modeling indicate that the deacylation-deficient Glu166Arg/Met182Thr mutant of TEM(

84 ephalosporin bound to the active site of the deacylation-deficient Q120L/Y150E variant of the class C

85 e same reactions are then reexamined using a deacylation-deficient variant, SHV E166A, that has been

86 ms, we have determined the structures of two deacylation-deficient variants (K84D and V130D) of the c

87 ey are active for acylation by substrate but deacylation-deficient.

88 s is serine acylation followed by hydrolytic deacylation, destroying the beta-lactam.

89 We therefore developed an assay to measure deacylation directly by pulse-chase incorporation of H(2

90 Gs) undergo rounds of inositol acylation and deacylation during GPI biosynthesis and the deacylation

91 However, the biological significance of LPS deacylation during infection of the mammalian host is un

92 he water molecule responsible for hydrolytic deacylation during normal hydrolysis.

93 carbonyl conjugation as a mechanism to avoid deacylation emerges despite that the penem and penam sul

94 th IleRS are consistent with a post-transfer deacylation event initiating formation of an editing-act

95 e of catalysis during both the acylation and deacylation events.

96 alog of the tetrahedral transition state for deacylation exhibits a very different binding geometry i

97 Both the acylation and deacylation follow a double-proton-transfer mechanism.

98 rase, these groups were removed by enzymatic deacylation followed by rapid chemical cyclization to 4,

99 -thiazepine derivative in OXA-1, and undergo deacylation followed by the decarboxylation of Lys-70, r

100 pproximately 60 min for K70A to undetectable deacylation for K70D.

101 gated, and a possible mechanism for the slow deacylation from KPC-2 is proposed.

102 The subsequent deacylation half-reaction is rate-limiting, with proton

103 microscopic events of both the acylation and deacylation half-reactions have not been studied.

104 from the relative rates of the acylation vs deacylation half-reactions of cathepsin C.

105 Methylamine (CH(3)NH(2))-mediated deacylation has previously been used in headgroup analys

106 gh "covalent trapping" of the substrate by a deacylation impaired enzyme with a lower K(m).

107 ld-type protein that was not detected in the deacylation impaired Glu166Asn mutant.

108 e, but our results do not support a role for deacylation in activity-dependent Galphas internalizatio

109 was more slowly subject to CD14-independent deacylation in AF.

110 s the enzyme responsible for G alpha subunit deacylation in S. cerevisiae and presumably other eukary

111 The faster rate of deacylation in the beta-lactamase is attributed to a mor

112 We show here that LPS undergoes deacylation in the liver and spleen by acyloxyacyl hydro

113 of the bacterial envelope is also subject to deacylation in the same inflammatory setting.

114 was used to confirm the position of lipid A deacylation in vitro and the release of the intact 3'-ac

115 Deacylation in vitro decreased activity of GPI-PLC 18-30

116 roduce AOAH and are required for hepatic LPS deacylation in vivo.

117 Deacylation interrupted HA-M1 interactions since deacyla

118 We find that long-chain deacylation is a general feature of mammalian sirtuins,

119 AOAH-mediated deacylation is a previously unappreciated mechanism that

120 sition of an active site water important for deacylation is altered compared with the wild-type enzym

121 complexes, this supports the hypothesis that deacylation is blocked by the continued presence of the

122 r, these results suggest that PagL enzymatic deacylation is posttranslationally inhibited by membrane

123 Ala-D-Ala peptide substrate, indicating that deacylation is rate determining for both amide and ester

124 for ceftazidime hydrolysis by KPC-2, whereas deacylation is rate-limiting in the R164S variant, leadi

125 on and acylation are viscosity-dependent but deacylation is viscosity-independent.

126 constants for both the acylation (k(2)) and deacylation (k(3)) of the extended-spectrum beta-lactama

127 inetic model where the acylation (k(ac)) and deacylation (k(dac)) half-reactions are very fast and si

128 active-site serine (k(acyl)), and hydrolytic deacylation (k(deacyl)).

129 ied when characterizing an unusual precursor deacylation mechanism during telomycin maturation.

130 over substrates, presumably via an acylation-deacylation mechanism.

131 Further, the selective biocatalytic deacylation methodology has been utilized for the effici

132 5, which upon Vorbruggen glycosylation, O2'-deacylation, O2'-activation and C2'-azide introduction y

133 Thus the different rates of deacylation occurred even though oncogenic and cellular

134 during incubation with ascitic fluid, no LPS deacylation occurred.

135 y accommodate a model of Tyr-tRNA(Phe) where deacylation occurs from either the 2'- or 3'-OH.

136 but also can be protein-dependent, and (iii) deacylation occurs in the endoplasmic reticulum immediat

137 (iii) Finally, deacylation occurs through a process involving a rate-li

138 Deacylation occurs with the formation of a tetrahedral i

139 ly of proteins catalyze the NAD(+)-dependent deacylation of acyl-lysine residues.

140 After deacylation of bovine brain sulfatide under mild alkalin

141 cylation or lactonization but did induce the deacylation of butyryl-acyl carrier protein.

142 14(+) MNC and a secondary role for AF in the deacylation of cell-free LPS at extravascular inflammato

143 transcriptional regulation of Cld1-mediated deacylation of CL influences energy metabolism by modula

144 10, were calculated and compared to that for deacylation of FAAH acylated by the substrate oleamide.

145 The subsequent inositol deacylation of fully mannosylated GPI intermediates is i

146 Experiments monitoring deacylation of Ile-tRNA(Leu) and misactivated adenylate

147 ner caspases, so that any free p20 formed by deacylation of initiator caspases cannot reassociate to

148 In this study, deacylation of isolated radiolabeled LPS by both cellula

149 Until now, the gene responsible for the 3-O-deacylation of lipid A among nitrogen-fixing endosymbion

150 xpression of PagL, an enzyme responsible for deacylation of lipid A, reducing its pro-inflammatory pr

151 provides support for the hypothesis that the deacylation of lipids on the Salmonella-containing vacuo

152 Deacylation of lipopolysaccharide occurred over several

153 Partial deacylation of LPS by the enzyme acyloxyacyl hydrolase (

154 Enzymatic deacylation of LPS is an intrinsic, regulated mechanism

155 Deacylation of LPS was always restricted to the secondar

156 ower than those of pre-ion-exchange LTA, and deacylation of LTA-10.5 by alkali hydrolysis reduces the

157 f RAT1 and XRN1 prevent both degradation and deacylation of mature tRNA(Val(AAC)) in a trm8-Delta trm

158 activities clear errors of aminoacylation by deacylation of mischarged tRNAs.

159 otential errors in protein synthesis through deacylation of mischarged tRNAs.

160 lation of 1-acylglycerol-3-P followed by the deacylation of monoacylglycerol.

161 or producing NAEs that involves the double-O-deacylation of N-acyl phosphatidylethanolamines (NAPEs)

162 hrough the serine hydrolase-catalyzed double-deacylation of NAPE to generate glycerophospho-NAE, foll

163 strate that C6orf130 catalyzes the efficient deacylation of O-acetyl-ADP-ribose, O-propionyl-ADP-ribo

164 We propose a catalytic mechanism for deacylation of O-acyl-ADP-ribose by C6orf130 and discuss

165 Novozyme-435-mediated diastereoselective deacylation of one of the two diastereotopic acyloxymeth

166 We propose that deacylation of PE and PC by phospholipase A(2) to genera

167 hocholine (GPC), the product of the complete deacylation of phosphatidylcholine (PC), was long though

168 se superfamily of enzymes which catalyze the deacylation of phospholipids.

169 MA-8H9D4 does not cause deacylation of preformed PAI-1/proteinase complexes and

170 Deacylation of purified lipopolysaccharides (LPS) marked

171 r measuring rate constants for acylation and deacylation of soluble penicillin binding protein (PBP)

172 a suggest a direct role for the SXN motif in deacylation of the acyl-enzyme complex and imply that th

173 talysis by these enzymes, namely, hydrolytic deacylation of the acyl-enzyme species, takes place effe

174 The rate constants k3 for deacylation of the acyl-PBP2a complexes, the third step

175 , PBP 5 is distinguished by its high rate of deacylation of the acylenzyme complex (t(1/2) approximat

176 ey are acylaminohydrolases that catalyze the deacylation of the amide-linked saturated fatty acid fro

177 ctamases, due in the class A enzymes to slow deacylation of the covalent acylenzyme intermediate, car

178 posed to solutions with no carbapenem, rapid deacylation of the Delta (2) species was observed by kin

179 rotein, and RNA, but also includes extensive deacylation of the envelope LPS.

180 Deacylation of the enzyme is rate-determining under subs

181 Deacylation of the enzyme is slow, e.g., 1.24 x 10(-)(3)

182 Inositol deacylation of the fully mannosylated GPI intermediate a

183 f inositol acylation and subsequent inositol deacylation of the GPI intermediates.

184 cal machinery required for the acylation and deacylation of the lipid A domain of H. pylori lipopolys

185 the ester, which would lead to unprogrammed deacylation of the peptidyl-tRNA.

186 Glycerophosphocholine is formed via the deacylation of the phospholipid phosphatidylcholine.

187 he peptide substrate, followed by hydrolytic deacylation of this acyl-enzyme intermediate to complete

188 he amino acid binding pocket failed to block deacylation of tRNA, indicating that the architecture of

189 yl-L-cysteine-, and dithiothreitol-dependent deacylations of aminoacyl-tRNA yield corresponding amino

190 The deacylation off-rate was 0.045 min(-1), which allowed in

191 vious studies investigated sirtuin-catalyzed deacylation on peptide substrates only.

192 all preferentially protonated at the oxygen, deacylation or dealkylation was observed in the collisio

193 mediate is fast relative to some later step (deacylation or N-terminal product release).

194 These data indicate that either deacylation or rearrangement of the enzyme-product compl

195 that are deficient in either aminoacylation, deacylation, or both, total editing (the sum of pre- and

196 choline kinase alpha (CHKA) and an activated deacylation pathway, as indicated by upregulated express

197 ate labeling, or on detecting only the final deacylation portion of the transglutaminase reaction.

198 rate-limiting at pH values less than 5.5 and deacylation principally rate-limiting above pH 8.5.

199 Using NMR and MS, we showed that deacylation proceeds through regeneration of intact avib

200 The rates of this deacylation process vary greatly depending on the serpin

201 rate constants (k(2)) of 1-26 s(-1), yet the deacylation process was essentially irreversible within

202 ydrolysis presumably by the normal acylation-deacylation process.

203 of lyso-PC and oleic acid, which constitute deacylation products of PC.

204 early 30-fold greater than the rate-limiting deacylation rate ( k dac = 13.95 +/- 0.013 s (-1)) and t

205 k2) is approximately 10-fold higher than the deacylation rate (k3).

206 h acetyl analog had a profound impact on the deacylation rate between deacetylase classes.

207 The deacylation rate constant for the PBP2a-penicillin G cov

208 Deacylation rate constants (k(3)) for the third step of

209 o the peripheral site decrease acylation and deacylation rate constants and/or decrease substrate aff

210 First-order deacylation rate constants could also be measured, as de

211 orresponding to over 70-fold increase of the deacylation rate for the resistant PBP2x(R).

212 p in wild-type PBP 5 markedly diminished the deacylation rate of penicillin G with a minimal impact o

213 Similarly, over 80-fold enhancement of the deacylation rate was found for cefotaxime-PBP2x(R) compl

214 at P(1) and the absence of an effect on the deacylation rate without involving mobility of the S(1)

215 Interestingly, BOCILLIN FL deacylation rates (t(1/2)) vary depending on the identit

216 is study, we characterized the acylation and deacylation rates and membrane trafficking of monoacylat

217 The origin of the substantial difference in deacylation rates for acyl-enzyme intermediates in penic

218 ane vesicular carriers, and 2) the different deacylation rates of single-acylated H-Ras influence dif

219 Complex deacylation rates were all slow, suggesting effective ki

220 In this work acylation and deacylation rates were measured against the clinically i

221 mplex and plasma membrane, but also in their deacylation rates, which we showed to be due to differen

222 ion, with opposite effects on acylation and deacylation rates.

223 itro approaches, we demonstrated that the PC deacylation reaction catalyzed by the reverse action of

224 icyclic intermediate for a sirtuin-catalyzed deacylation reaction that has been captured in a crystal

225 release of P1, and a general base-catalyzed deacylation reaction.

226 ck trajectory of the hydrolytic water in the deacylation reaction.

227 sitioned to act as the catalytic base in the deacylation reaction.

228 sp2) bond formation followed by a subsequent deacylation reaction.

229 s used to measure the rate constant for this deacylation reaction.

230 deacylation during GPI biosynthesis and the deacylation reactions are inhibited by diisopropylfluoro

231 sed to model the relative reaction rates for deacylation reactions for aliphatic and aromatic ester s

232 kely to be the active catalyst for the ester deacylation reactions under ammonium acetate mediated co

233 hin 2h at 260 degrees C), dechlorination and deacylation reactions.

234 orporation into islet phospholipids involved deacylation-reacylation and not de novo synthesis, as in

235 part through a remodeling mechanism via the deacylation-reacylation cycle mediated by phospholipase

236 of phospholipid molecules, or remodeling by deacylation-reacylation may be important contributors in

237 in alveolar type II cells de novo or by the deacylation-reacylation of existing phosphatidylcholine

238 tate metabolic integration into triglyceride deacylation-reacylation pathways.

239 biochemical incorporation into triglyceride deacylation-reacylation pathways.

240 he sphingosine backbone of C(6)-ceramide via deacylation/reacylation and not due to the elongation of

241 The successful operation of this deacylation/reacylation cycle is important for Giardia b

242 ids in dual-labeled cells indicated that the deacylation/reacylation cycle was the major route of AA

243 ts suggest that Giardia is able to carry out deacylation/reacylation reactions (the Lands cycle) to g

244 well as PLA2 (in doses simulating Fe-induced deacylation) recapitulated Fe's ceramide-generating effe

245 min(-1), which allowed investigation of the deacylation route from TEM-1.

246 stead characteristic of a hydroxide-mediated deacylation similar to that observed for the hydrolysis

247 the 2'-OH for both enzymes, IleRS catalyzes deacylation specifically from the 3'-OH and not from the

248 rolysis pathway, consisting of acylation and deacylation stages similar to those for ester hydrolysis

249 t the rate-limiting step in turnover was the deacylation step (governed by k(3)) or that neither the

250 These data indicate that the deacylation step also plays a role, which is much more i

251 y of the tetrahedral intermediate during the deacylation step in elastase-catalyzed hydrolysis of a s

252 The free energy of activation for the deacylation step is 16.7 kcal/mol in cathepsin K and 17.

253 assignment as the 'hydrolytic water' in the deacylation step of serine protease catalysis.

254 glutamate residue that has a key role in the deacylation step of the catalytic mechanism, allowing th

255 the transition-state intermediate during the deacylation step of the enzyme-catalyzed reaction with p

256 itions of tRNA isolation include an alkaline deacylation step that also causes hydrolysis of glutamyl

257 amine, to act as the hydrolytic water in the deacylation step.

258 of 1.6 on the acylation step and 3.4 on the deacylation step.

259 Both the acylation and deacylation steps are of equal magnitude.

260 vealed large SKIEs on both the acylation and deacylation steps of 3.8 and 4.0, respectively.

261 an important role in both the acylation and deacylation steps of the catalytic mechanism.

262 eral base residue for both the acylation and deacylation steps of the enzyme.

263 act as a general base in both acylation and deacylation steps of the reaction.

264 is slow enough to resolve the acylation and deacylation steps on the catalytic pathway.

265 l free energy surfaces of both acylation and deacylation steps to characterize the reaction mechanism

266 can readily form Cys- and Ala-tRNA(Pro), and deacylation studies confirmed that these species are cle

267 why the sirtuin-dependent protein acylation/deacylation system (SDPADS) controls the activity of Acs

268 ntial-energy surface for the collapse of the deacylation tetrahedral species gives a 24 kcal x mol(-1

269 ubstrate-assisted mechanism of Cys-tRNA(Pro) deacylation that prevents nonspecific Pro-tRNA(Pro) hydr

270 se data suggest a mechanism of catalysis for deacylation that uses a hydrogen-bonding network, involv

271 Therefore, in addition to impairing deacylation, the acylation machinery has been altered co

272 the latter results from general catalysis of deacylation, the former originates purely from the react

273 haracteristic of His-57 catalysis of complex deacylation, the pH dependence of k(diss, app) for the s

274 e rate-limiting step for ALDH1, and enhanced deacylation, the rate-limiting step for ALDH2.

275 olibactins are converted to colibactins by N-deacylation; the latter are postulated to be genotoxic a

276 inity and kinetics of binding to the chiral, deacylation transition state inhibitor.

277 s the need for general base catalysis in the deacylation transition state of the Streptomyces R61 DD-

278 o inhibit by preventing the formation of the deacylation transition state through steric hindrance.

279 tion state to its acyl enzyme complex to the deacylation transition state.

280 on transition state and those that mimic the deacylation transition state; they also suggest how TEM-

281 Differences in deacylation transition states (V) between the two enzyme

282 Deacylation transition states of both enzymes appear to

283 of catalysis (acyl-enzyme and acylation and deacylation transition states), whereas the beta-lactama

284 eta-lactamase in complex with a boronic acid deacylation transition-state analogue.

285 d nucleoside derivatives undergo selective N-deacylation upon heating at elevated temperatures (oil b

286 varying either the rates of RCL insertion or deacylation using a library of serpin RCL mutants substi

287 PagL-dependent deacylation was detected in sonically disrupted membrane

288 being changed from coenzyme dissociation to deacylation was finding that chloroacetaldehyde was oxid

289 g step for cephalosporin substrates, whereas deacylation was rate-limiting for penicillin substrates.

290 s was used to determine whether acylation or deacylation was rate-limiting.

291 red anchoring and decreased occupancy of the deacylation water explain the lower k(cat) values of Pen

292 donated to the boronic oxygen mimicking the deacylation water.

293 drolysis of (+)-cocaine is the first step of deacylation, whereas for (-)-cocaine the change from the

294 rface proteins is regulated via posttransfer deacylation, which in general is cell-specific but also

295 Extended heating is required for N-deacylation with arylcarboxylic acid derivatives.

296 5 --> Asp mutation in PBP 5 markedly impairs deacylation with only minor effects on acylation, and ab

297 gested that dechlorination is preferred over deacylation with the conditions applied in this study.

298 Deacylations with Li+ -OCH2CF3/TFE proceed at ambient te

299 -10 displayed the longest residence time for deacylation, with a half-life of greater than 5 days.

300 ons, almost all MNC-associated LPS underwent deacylation within 1 h, a rate greatly exceeding that pr