ライフサイエンスコーパス: deadenylation

1 ich is initiated by poly(A) tail shortening (deadenylation).

2 stimulates removal of its poly(A) tail (i.e. deadenylation).

3 rotects RNA(3'-rA) from ligation and further deadenylation.

4 domain of PAB1 blocked both their effects on deadenylation.

5 caf1b, AtCAF1b targets AtPI4Kgamma3 mRNA for deadenylation.

6 bunits CAF1 and CCR4 as factors required for deadenylation.

7 capping/5'-to-3' or 3'-to-5' direction after deadenylation.

8 RNAs, premature termination codons, and mRNA deadenylation.

9 larization, correspondingly accelerated CCR4 deadenylation.

10 ors involved in translation are required for deadenylation.

11 PAB1 oligomerization is a required step for deadenylation.

12 binding protein (PAB1) as necessary for CCR4 deadenylation.

13 4p and Pop2p deadenylases in Mpt5p-regulated deadenylation.

14 ntially regulate transcripts at the level of deadenylation.

15 zyme to the target mRNA, thereby stimulating deadenylation.

16 d mRNA decay initiated by miRNA-guided rapid deadenylation.

17 anced mt-mRNA decay by translation-dependent deadenylation.

18 residue predicted to bind magnesium disrupts deadenylation.

19 ynthetic RNA substrate in a process known as deadenylation.

20 transcripts, apparently by initiating their deadenylation.

21 vo for controlling the rate of specific mRNA deadenylation.

22 ns, contacting SAGA and contributing to mRNA deadenylation.

23 A specific mechanism triggers this deadenylation.

24 cal for determining the rate of ARE-mediated deadenylation.

25 stabilized mCRD-containing mRNA by impeding deadenylation.

26 of mRNA turnover to elucidate mechanisms of deadenylation.

27 ciated factor 1 (CAF1), is a major player in deadenylation.

28 xpression stabilized TNF mRNA by suppressing deadenylation.

29 3'-termini, consistent with protection from deadenylation.

30 g evidence indicates that TOBs regulate mRNA deadenylation.

31 contains multiple enzymes that catalyze mRNA deadenylation.

32 nanos1 and TDRD7 from miR-430b-mediated RNA deadenylation.

33 3 acts through a general mechanism to affect deadenylation.

34 F1 also represses translation independent of deadenylation.

35 is sufficient for repression, independent of deadenylation.

36 nd to be required for eIF4E effects on COX17 deadenylation.

37 tibility to mutations inhibiting cytoplasmic deadenylation, a process required for both 5'-to-3' and

38 trolled by a member of the Pumilio family of deadenylation activators Puf3p, requires an active Ccr4p

39 quitination of CNOT7 by MEX-3C regulates its deadenylation activity and is required for MHC-I mRNA de

40 This 3'-deadenylation activity is metal-dependent and requires a

41 n extensively characterized exonuclease with deadenylation activity that controls mRNA stability in p

42 crystal structures are essential for the 3'-deadenylation activity, suggesting that 3'-adenosine may

43 evaluation of patient cells revealed reduced deadenylation activity.

44 Deadenylation, also called poly(A) tail shortening, is t

45 Deadenylation, also called poly(A) tail shortening, is t

46 niaD transcript are effected at the level of deadenylation and are dependent on its 3' UTR.

47 results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during ear

48 mRNA is sufficient for both protection from deadenylation and deadenylation-independent decapping an

49 ch suggests a possible link between the mRNA deadenylation and decapping machinery.

50 en established as a target of Puf3p-mediated deadenylation and decapping.

51 The ENE inhibits deadenylation and decay in nuclear extract and prevents

52 scription-pulsing approaches to monitor mRNA deadenylation and decay kinetics, we demonstrate the exi

53 rameric DST element from soybean accelerated deadenylation and decay of a reporter transcript.

54 Drosophila Tis11 promoted both deadenylation and decay of a target transcript in this h

55 the beta-globin reporter mRNA promoted rapid deadenylation and decay of hypo-adenylated reporter mRNA

56 TTP is known to stimulate the deadenylation and decay of mRNAs possessing one or more

57 Accelerated deadenylation and decay of the transcript follows riboso

58 We observed that during the biphasic deadenylation and decay process of this mRNA, dTIS11 enh

59 m whereby TTP targets inflammatory mRNAs for deadenylation and decay.

60 elements in target mRNAs, and promote their deadenylation and decay.

61 ch regions in target mRNAs, leading to their deadenylation and decay.

62 eorganizes the complex, leading to rapid RNA deadenylation and decay.

63 sed binding to RNA as well as inhibited mRNA deadenylation and decay.

64 ich elements in mRNAs and promote transcript deadenylation and decay.

65 nitiation of target mRNAs, followed by their deadenylation and decay.

66 ional repression and/or messenger RNA (mRNA) deadenylation and decay.

67 slational inhibition, followed by effects on deadenylation and decay.

68 They target mRNAs for rapid deadenylation and degradation and may enhance decapping.

69 d alterations in activities that govern both deadenylation and degradation of the mRNA body.

70 as the mRNA encoding TNF, and promotes their deadenylation and degradation.

71 ession and/or promoting messenger RNA (mRNA) deadenylation and degradation.

72 many maternal proteins are downregulated by deadenylation and destabilization of their encoding mRNA

73 taining AU-rich elements (ARE), resulting in deadenylation and destabilization of these transcripts.

74 elements within certain mRNAs, resulting in deadenylation and destabilization of those mRNAs.

75 in certain cellular mRNAs, leading to their deadenylation and destabilization.

76 h elements (AREs) in mRNAs, leading to their deadenylation and destabilization.

77 s, leading to increases in the rates of mRNA deadenylation and destruction.

78 rveillance complex can target mRNAs to rapid deadenylation and exosome-mediated degradation.

79 This transition occurs after deadenylation and includes loss of Pab1p, eIF4E, and eIF

80 s reducing the ability of the TTP to promote deadenylation and instability of the mRNA.

81 the major enzyme complex that catalyzes mRNA deadenylation and is conserved among eukaryotes.

82 ping and distinct roles with respect to mRNA deadenylation and mediation of stress responses.

83 with a functional assay directly monitoring deadenylation and mRNA decay to characterize the effects

84 a Caf1 catalytically inactive mutant impairs deadenylation and mRNA decay.

85 rent levels by regulating pre-mRNA splicing, deadenylation and mRNA stability.

86 We find that TGEs accelerate the rate of deadenylation and permit the last 15 adenosines to be re

87 upregulated after infection but degraded by deadenylation and progressive 3'-to-5' degradation.

88 While required for target mRNA deadenylation and silencing, this site is not sufficient

89 or bcd NRE mutations caused delayed bcd mRNA deadenylation and stabilization, resulting in protracted

90 decapping-independent NMD pathway involving deadenylation and subsequent 3'-->5' exonucleolytic deca

91 stabilizes host stress response mRNAs after deadenylation and subsequent cleavage near the adenylate

92 rated to direct target RNA reduction through deadenylation and subsequent degradation of target trans

93 translated region of mRNAs, leading to their deadenylation and subsequent degradation.

94 Exit from M phase seems to require deadenylation and subsequent translational silencing of

95 decay of this mRNA by enhancing the rate of deadenylation and subsequent turnover.

96 Furthermore, transcript deadenylation and the consequent dissociation of poly(A)

97 ls and is thus a potential regulator of mRNA deadenylation and translation during early development.

98 that the interplay between polyadenylation, deadenylation and tumour-suppressor factors might preven

99 t encoding TNF, and increases their rates of deadenylation and turnover.

100 ed regions (UTRs) and subsequently promoting deadenylation and ultimate destruction of those mRNAs.

101 unique properties and is both protected from deadenylation and undergoes deadenylation-independent de

102 Because translation, deadenylation, and decay are closely linked processes, i

103 nslational repression occurs before complete deadenylation, and disrupting deadenylation with use of

104 untranslated region and recruits decapping, deadenylation, and exonucleolytic enzymes to PBs for RNA

105 ranslational repression precedes target mRNA deadenylation, and identify GW182, PABP, and deadenylase

106 mRNAs that affects translation termination, deadenylation, and mRNA decay.

107 Aly appears to protect the poly(A) tail from deadenylation, and REF/Aly-stabilized transcripts are fu

108 We also find that TOBs' actions in deadenylation are independent of the phosphorylation sta

109 role for ubiquitin in regulating MHC-I mRNA deadenylation as ubiquitination of CNOT7 by MEX-3C regul

110 Deadenylation assays confirm the functional importance o

111 tion assays and in cell-free RNA binding and deadenylation assays, suggesting that it may play roles

112 P domain) of PAB1 substantially reduces CCR4 deadenylation at non-PUF3-controlled mRNA and correspond

113 ression through translational repression and deadenylation but not cleavage.

114 PABPs accelerate miRNA-mediated deadenylation, but this contribution can be modulated by

115 structure in the 5' UTR dramatically reduces deadenylation by interfering with cap-DAN interactions.

116 The regulation of deadenylation by p38 MAPK was found to be specific becau

117 nhibition of 3' cleavage, strongly activates deadenylation by PARN in the presence of CstF-50, and th

118 ent model was proposed in which TOBs promote deadenylation by recruiting CAF1-CCR4 deadenylase comple

119 so implicated in mediating the repression of deadenylation by the 3' UTR of another alphavirus, Venez

120 trate polyA RNA, facilitating efficient mRNA deadenylation by the intact Pan2-Pan3 complex.

121 hat reduced translation, while not affecting deadenylation by themselves or when combined with ccr4De

122 -adenylated intermediates are substrates for deadenylation by yeast 5'Deadenylase.

123 mRNA was stabilized in mutants defective in deadenylation (ccr4Delta), mRNA decapping (dcp1), and th

124 CCR4-CAF1-NOT complex is a major cytoplasmic deadenylation complex in yeast and mammals.

125 The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA

126 tin ligases, associates with the cytoplasmic deadenylation complexes and ubiquitinates CNOT7(Caf1), t

127 the predominant effect of microRNA-mediated deadenylation concurrently shifts from translational rep

128 em zinc finger domain; it then promotes mRNA deadenylation, considered to be the rate-limiting step i

129 y in Saccharomyces cerevisiae occurs through deadenylation, decapping, and 5' to 3' degradation of th

130 he major mRNA turnover pathway that requires deadenylation, decapping, and 5'-to-3' exonucleolytic de

131 that inhibit translation and stimulate mRNA deadenylation, decapping, and decay.

132 of mRNA decay enzymes, and help explain how deadenylation, decapping, and exonucleolytic decay can a

133 ough a large variety of mechanisms including deadenylation, decapping, and P-body targeting.

134 AREs have been shown to directly activate deadenylation, decapping, or 3'-to-5' exonucleolytic dec

135 Using an in vitro RNA deadenylation/decay assay, mRNA decay intermediates were

136 anding/assembly" platform for formation of a deadenylation/decay mRNA-protein complex on an mCRD-cont

137 This deadenylation deficiency caused an early DNA damage resp

138 tion of even a small excess of ePAB inhibits deadenylation, demonstrating that the ePAB concentration

139 A degradation and repress expression by both deadenylation-dependent and -independent mechanisms, usi

140 Thus, human PUMs are repressors capable of deadenylation-dependent and -independent modes of repres

141 or pathway of eukaryotic mRNA decay involves deadenylation-dependent decapping followed by 5' to 3' e

142 antly, both yeast and mammalian AREs promote deadenylation-dependent decapping in the yeast system.

143 the major mRNA decay mechanism in yeast, the deadenylation-dependent decapping pathway.

144 nger RNA degradation in eukaryotes occurs by deadenylation-dependent decapping which leads to 5'-to-3

145 The ENE protects PAN RNA from a rapid deadenylation-dependent decay pathway via formation of a

146 h1 specifically affects mRNA turnover in the deadenylation-dependent decay pathway, but does not act

147 s the 5' cap-structure of mRNA and initiates deadenylation-dependent decay.

148 ound that while Olfr mRNAs are degraded by a deadenylation-dependent mechanism, they are largely prot

149 een studied extensively and is degraded by a deadenylation-dependent mechanism.

150 nd other components of the Lsm1p-Lsm7p/Pat1p deadenylation-dependent mRNA decapping complex were also

151 ody-associated 5' to 3' mRNA decay pathways, deadenylation-dependent mRNA decay (DDD) and nonsense-me

152 Therefore, our data uncover a new, deadenylation-dependent mtRNA maturation pathway in huma

153 e virus has evolved a way to avoid the major deadenylation-dependent pathway of mRNA decay.

154 res a 5' cap structure on the mRNA; however, deadenylation does not.

155 TOE1 displays a functional deadenylation domain and has been shown to participate i

156 teraction with PABPC1 is necessary for TOB's deadenylation-enhancing effect.

157 pathways of eukaryotic mRNA decay occur via deadenylation followed by 3' to 5' degradation or decapp

158 yotic mRNA decay proceeds through an initial deadenylation followed by 5' end decapping and exonucleo

159 f eukaryotic mRNA degradation initiates with deadenylation followed by decapping and 5' to 3' degrada

160 t, a major pathway of mRNA decay begins with deadenylation followed by decapping and 5'-3' exonucleas

161 major pathway for mRNA decay is initiated by deadenylation followed by decapping and 5'-3' exonucleol

162 sequence-specific manner, resulting in mRNA deadenylation followed by exonucleolytic decay, mRNA end

163 e generalized process of mRNA decay involves deadenylation followed by release from translating polys

164 ic messenger RNA (mRNA) turnover begins with deadenylation, followed by decapping and 5' to 3' exonuc

165 athway of mRNA decay in yeast initiates with deadenylation, followed by mRNA decapping and 5'-3' exon

166 ivity, this activity is not required for its deadenylation function in vivo, and CCR4 is the primary

167 vo effect of AU-rich elements (AREs) on mRNA deadenylation has been developed from Xenopus activated

168 Since deadenylation has been reported to be required for P bod

169 e major pathway of RNA degradation following deadenylation in HeLa cytoplasmic extracts.

170 that OsCAF1 proteins may be involved in the deadenylation in rice.

171 Stabilization occurs as a result of slower deadenylation in the ssa1(ts) strain, suggesting that Hs

172 The rate of deadenylation in vitro by yCCR4 and mCAF1 were both stro

173 ) interact with the polyA tail, (ii) inhibit deadenylation in vitro, and (iii) stabilize transcripts

174 he CCF-1/Pop2p deadenylase and can stimulate deadenylation in vitro, and that CCF-1 is partially resp

175 reases stability in vivo but does not affect deadenylation in vitro, comparable to the effects of del

176 es the rate of both ARE-mediated and default deadenylation in vitro.

177 nuclease involved in the mCRD-mediated rapid deadenylation in vivo and also associated with UNR.

178 s support the model that the control of CCR4 deadenylation in vivo occurs in part through the removal

179 AF1-specific functional regions required for deadenylation in vivo, we targeted for mutagenesis six r

180 ferative transcription factor, enhances mRNA deadenylation in vivo.

181 om the poly(A) tail may be rate limiting for deadenylation in vivo.

182 Ccr4p and Caf1p are required for normal mRNA deadenylation in vivo.

183 roteins are required for full repression and deadenylation in vivo; their removal dramatically stabil

184 oupled from mRNA body decay, and the rate of deadenylation increases with the number of tandem AUUUAs

185 A second, deadenylation independent mechanism was revealed by the

186 t for both protection from deadenylation and deadenylation-independent decapping and an extended poly

187 cp2p to the complex appears to ensure rapid, deadenylation-independent decapping of the mRNA.

188 pts are efficiently recognized, targeted for deadenylation-independent decapping, and show NMD trigge

189 h protected from deadenylation and undergoes deadenylation-independent decapping.

190 ion codons (nonsense mRNAs) are targeted for deadenylation-independent degradation in a mechanism tha

191 BP delta mRNA degradation, which suggested a deadenylation-independent pathway.

192 egradation of the mRNA body, TPA activates a deadenylation-independent pathway.

193 The deadenylation-independent repression requires a 5' cap s

194 mRNA deadenylation is a key process in the regulation of tran

195 P-bodies are not detected when deadenylation is blocked and are restored when the block

196 When deadenylation is impaired, P-body formation is not resto

197 Transcript is stabilized when accelerated deadenylation is impeded by blocking translation initiat

198 In Saccharomyces cerevisiae, deadenylation is primarily carried out by the Ccr4p and

199 We demonstrate that deadenylation is required for mammalian P-body formation

200 Deadenylation is the first and rate-limiting step in the

201 One consequence of deadenylation is the formation of nontranslatable messen

202 Deadenylation is the major step triggering mammalian mRN

203 ARE-mediated deadenylation is uncoupled from mRNA body decay, and the

204 mRNA deadenylation is under the control of cis-acting regulat

205 , the main catalytic subunit of the CCR4-NOT deadenylation machinery.

206 RNA binding by preventing recruitment of the deadenylation machinery.

207 s suggest that inhibition of splicing and/or deadenylation may be effective therapies for Lsm1-over-e

208 protein-were implicated in the repression of deadenylation mediated by the SINV 3' UTR.

209 We found that degradation occurs via mRNA deadenylation, mediated by the CCR4-NOT complex.

210 ly(A) tail exacerbate dependency on PABP for deadenylation, more potent miRNA-binding sites partially

211 se results support a dynamic interplay among deadenylation, mRNP remodeling, and P-body formation in

212 RNase H treatment indicated that rapid deadenylation occurred concomitant with degradation of t

213 uration of mutated RNA14 was unaffected, but deadenylation occurred rapidly.

214 binds to RNAs containing AREs, and promotes deadenylation of a model ARE transcript in a cell-based

215 We conclude that TTP can promote the deadenylation of ARE-containing, polyadenylated substrat

216 TTP and its related proteins stimulated the deadenylation of ARE-containing, polyadenylated transcri

217 TTP may remain poised to rapidly reactivate deadenylation of bound transcripts to downregulate gene

218 hypothesis where mTOR is the tag, preventing deadenylation of CaMKIIalpha mRNA, whereas HuD captures

219 sults strongly suggest that zygote-dependent deadenylation of cyclin A1 and cyclin B2 mRNAs is respon

220 4p proteins are required for Mpt5p-regulated deadenylation of HO.

221 ne hydrolase (MTAN) catalyzes the hydrolytic deadenylation of its substrates to form adenine and 5-me

222 These findings indicate that following deadenylation of mammal mRNA, degradation proceeds by a

223 to CAF1, plays an essential role in the CCR4 deadenylation of mRNA.

224 nopus oocytes and early embryos and prevents deadenylation of mRNAs, suggesting its importance in the

225 on and decay in nuclear extract and prevents deadenylation of naked RNA by a purified deadenylase, li

226 p38 MAPK activation inhibited the deadenylation of reporter mRNAs containing either the cy

227 do not directly participate in the catalytic deadenylation of ribosomal RNA, play a critical role in

228 ) that directly participate in the catalytic deadenylation of RNA resulted in greater than 3 logs of

229 Destabilization and deadenylation of RP transcripts were impaired in an rpb4

230 Regulated polyadenylation and deadenylation of specific mRNAs is involved in oogenesis

231 TTP can direct the deadenylation of substrate mRNAs when tethered to a hete

232 of TTP and its family members to promote the deadenylation of such transcripts in intact cells.

233 cytoplasmic deadenylases and promotes rapid deadenylation of target mRNAs both in vitro and in cells

234 We also show that miR-430 accelerates the deadenylation of target mRNAs.

235 by p38 MAPK was found to be specific because deadenylation of the beta-globin reporter mRNA either la

236 PUF3 was shown to affect PAN2 deadenylation of the COX17 mRNA independent of the prese

237 In addition, the rapid deadenylation of the COX17 mRNA, which is controlled by

238 PUF3 in Saccharomyces cerevisiae accelerates deadenylation of the COX17 mRNA.

239 s, and this has been shown to be mediated by deadenylation of the mRNA and inhibition by the Bruno re

240 ion intermediates revealed that the complete deadenylation of the mRNA triggers its decapping and dec

241 caused in part by a decrease in the rate of deadenylation of the mRNA.

242 binding of TTP to the RNA and the subsequent deadenylation of the poly(A) tail.

243 o be due to inhibition of polyadenylation or deadenylation of the transcript, followed by exosomal de

244 A1 and B2 proteins is preceded by the rapid deadenylation of their mRNAs.

245 em CCCH zinc finger protein family, promotes deadenylation of tumor necrosis factor-alpha and granulo

246 responsible for mediating the repression of deadenylation of viral mRNAs.

247 This block in deadenylation of viral transcripts was recapitulated in

248 unction as microRNA (miRNA) mimics in either deadenylation or guided mRNA cleavage (RNAi).

249 ails prematurely either as a result of rapid deadenylation or reduced polyadenylation.

250 To investigate whether it regulates deadenylation or the decay of the mRNA body, we used a t

251 nscripts, thereby causing their degradation, deadenylation, or inhibiting their translation.

252 decay process of this mRNA, dTIS11 enhances deadenylation performed by the CCR4-CAF-NOT complex whil

253 The mRNA deadenylation process, catalyzed by the CCR4 deadenylase

254 gement in mRNP organization and suggest that deadenylation promotes mRNA decapping by both the loss o

255 unclear whether TOBs' antiproliferative and deadenylation-promoting activities are connected.

256 l a link between TOBs' antiproliferative and deadenylation-promoting activities.

257 and correspondingly blocked eIF4E effects on deadenylation, PUF3 essentially bypassed this P domain r

258 o shape maternal mRNA stability by affecting deadenylation rate in a translation-dependent manner.

259 of the NOT genes can lead to defects in mRNA deadenylation rates.

260 the p38 MAPK pathway predominantly regulates deadenylation, rather than decay of the mRNA body, and t

261 ecapping, and show NMD triggered accelerated deadenylation regardless of the position of the nonsense

262 Since deadenylation regulates mRNA decay and/or translational

263 molecular mechanism underlying TOB-promoted deadenylation remains unclear.

264 cludes three distinct steps: a rate-limiting deadenylation, removal of the 5'-7-methyl-G (decapping),

265 reverses DNA adenylation but the context for deadenylation repair is unclear.

266 MicroRNAs regulate gene expression through deadenylation, repression, and messenger RNA (mRNA) deca

267 The ability of TTP to promote transcript deadenylation required Mg(2+), but not ATP or prior capp

268 nuclease activity resides in Pan2, efficient deadenylation requires Pan3.

269 hrough its contact to CCR4, has functions in deadenylation separate from its contact to CCR4.

270 In yeast, the NMD pathway bypasses the deadenylation step and directly targets PTC-containing m

271 The regulation of the critical deadenylation step and its relationship with RNA-process

272 e fate of mammalian mRNA is modulated at the deadenylation step by a protein that recruits poly(A) nu

273 d for and regulates global mRNA decay at the deadenylation step in Saccharomyces cerevisiae.

274 mediated by the mCRD, demonstrating that the deadenylation step is coupled to ongoing translation of

275 ii) the hCaf1z subunit, in addition to rapid deadenylation, subjects substrate RNAs to slow exonucleo

276 thway, a nonsense codon triggers accelerated deadenylation that precedes decay of the PTC-containing

277 ntify a new role for ubiquitin in regulating deadenylation, the initial and often rate-limiting step

278 y conserved PUF proteins stimulate CCR4 mRNA deadenylation through binding to 3' untranslated region

279 ese results imply that CAF1, while affecting deadenylation through its contact to CCR4, has functions

280 ed for P body formation, viral inhibition of deadenylation, through Pan3 degradation, is a potential

281 quired for binding CCR4, reduced the rate of deadenylation to a lesser extent and resulted in in vivo

282 -bodies, suggesting a role of TOB in linking deadenylation to the P-bodies.

283 he genes in vertebrates, where they regulate deadenylation, translation, and decay of the target mess

284 inhibition of 3' cleavage and activation of deadenylation upon DNA damage.

285 ochondrial protein synthesis showed that the deadenylation was dependent on translation.

286 Transcript deadenylation was not stimulated when a mutant TTP prote

287 Finally, deadenylation was shown to enhance mRNA decay, explainin

288 tion initiation factor known to control CCR4 deadenylation, was shown to affect PAN2 activity in vivo

289 Both eIF4E and PUF3 effects on deadenylation were shown, in turn, to necessitate a func

290 en combined with ccr4Delta, severely blocked deadenylation when coupled with a caf1 deletion.

291 hanisms: repression by Puf4p is dependent on deadenylation, whereas repression by Mpt5p can occur thr

292 which binds only to monomeric Orb2, promotes deadenylation, whereas the putative poly(A) binding prot

293 In mammalian cells, mRNA decay begins with deadenylation, which involves two consecutive phases med

294 However, little is known about rice mRNA deadenylation, which is an important regulation step of

295 mRNA degradation is often initiated by deadenylation, which leads to decapping and 5'-3' decay.

296 ne major pathway for mRNA turnover occurs by deadenylation, which leads to decapping and subsequent 5

297 ge abundance is a consequence of accelerated deadenylation, which leads to rapid mRNA decay.

298 Following deadenylation with nicotinamide mononucleotide, the puri

299 F1b target shared and unique transcripts for deadenylation with temporal specificity.

300 efore complete deadenylation, and disrupting deadenylation with use of an internal polyadenylate tail