ライフサイエンスコーパス: deamidate

1 tion for the peptide in which the residue is deamidated.

2 The deamidated 33-mer peptide efficiently exchanges with a p

3 At pH 5.5 and 7.3, dissociation of the deamidated 33-mer peptide from DQ2 is much slower than d

4 In addition, the deamidated 3C protease was found to be present in vivo,

5 a coli, acts on Rho-GTPases and specifically deamidates a single glutamine residue (Gln-63 in RhoA) r

6 ytic triad, which inactivates Rho GTPases by deamidating a conserved asparagine in the GTPase switch-

7 activates small GTPases of the Rho family by deamidating a single amino acid within these target prot

8 -tTG(553-564)-NH2 sequence cross-linked with deamidated Ac-alpha2-Glia(63-71)-NH2, was able to identi

9 Human WT-alphaA and human deamidated alphaA (alphaA-N101D, alphaA-N123D, alphaA-N1

10 deletion of the C-terminal extension in the deamidated alphaA mutants, but on N-terminal domain dele

11 xchange rate than heteroaggregate containing deamidated alphaA- and WT-alphaB subunits.

12 alphaA-crystallin, the lenses containing the deamidated alphaA-crystallin also showed an aggregation

13 idence for the first time that expression of deamidated alphaA-crystallin caused disruption of fiber

14 the heteroaggregate containing WT-alphaA and deamidated alphaB subunit showed lower chaperone activit

15 Deamidated alphaB-crystallin mutants (N78D, N146D, and N

16 The triply deamidated analog also aggregated into amyloid fibrils f

17 ic enzyme activity, recruit cellular PFAS to deamidate and activate RIG-I.

18 o play a role in the repair or metabolism of deamidated and isomerized proteins that are spontaneousl

19 Pup (prokaryotic ubiquitin-like protein) is deamidated and isopeptide linked to proteins by a mechan

20 luten-reactive T cells in children recognize deamidated and native gluten epitopes, whereas T cells f

21 laevis oocytes were microinjected with both deamidated and nondeamidated forms of recombinant chicke

22 dic (lower pI) peaks were found to represent deamidated and sialyated species.

23 a highly conserved cysteine proteinase that deamidates and inactivates the anticancer drug bleomycin

24 physiological roles for the two alternative, deamidating and nondeamidating, routes of nicotinamide s

25 The deamidated antibody variants were less potent in antigen

26 esidues at positions 79, 141 and 187 and one deamidated Asn residue at position 176, were detected at

27 irmed, by mass spectroscopy, the presence of deamidated (Asp(64)) and native (Asn(64)) COMP epitopes

28 L-ASP from Escherichia coli deamidates asparagine (Asn) and glutamine, with an ~10(4

29 MM28, MM50, and Mel202 cells failed to deamidate Bcl-x(L) in response to radiation-induced DNA

30 Additionally, we show that degradation of deamidated Bcl-xL is mediated at least in part by calpai

31 71 and Asn94 react; these are more than half deamidated before Asn34 reacts, and its deamidation is e

32 of ubiquitination and degradation of WT and deamidated betaB1 crystallins were rapid and showed litt

33 ive 10-mer peptides were inactive, even when deamidated, but V96F substitution of deamidated TFIIA am

34 aragine residues 246 and 259 were completely deamidated by age 7 years.

35 Second, they are recognized and deamidated by human tissue transglutaminase (tTGase) wit

36 astrointestinal digestion and that have been deamidated by tissue transglutaminase.

37 TG2 deamidates certain gluten peptides, increasing their aff

38 d native (Asn(64)) COMP epitopes (mean 0.95% deamidated COMP (D-COMP) relative to native COMP) in car

39 Wild-type (WT) and deamidated crystallins were expressed and (125)I-radiola

40 d residue Asn55 in vivo and the ratio of the deamidated derivatives, i.e., isoAsp55 and Asp55.

41 In conclusion, both Cp NGR sites can deamidate during aging under oxidative conditions, likel

42 ns are crystallins, and they are extensively deamidated during aging and cataracts.

43 he yeast Saccharomyces cerevisiae, which can deamidate either N-terminal asparagine or glutamine.

44 mportant component of the disease, both as a deamidating enzyme that can enhance the immunostimulator

45 We believe that enrichment of deamidated epitope in hip OA cartilage indicates a lesse

46 Recombinant deamidated ErA mutants were also demonstrated to migrate

47 In contrast, the hidden (962)NGR site can deamidate exclusively when aging occurs under oxidative

48 s protein (which is not associated with DNA) deamidated fairly readily in spores (t(1/2), approximate

49 is indicated that the pI 8.3 protein was the deamidated form of 3C, and it displayed approximately 10

50 ne immunogenicity, we created a "genetically deamidated" form of rPA using site-directed mutagenesis

51 ), and gammaS (52-71)) and their potentially deamidated forms as model peptides.

52 HPLC isolation of various deamidated forms followed by peptide mapping and mass sp

53 identify and quantitate the amidated versus deamidated forms of each tryptic fragment of alpha-A cry

54 of tryptic fragments containing amidated and deamidated forms using high pressure liquid chromatograp

55 tic-tryptic digest of gliadin (in native and deamidated forms) before T-cell collection.

56 ntified omega- and gamma-gliadins, and their deamidated forms, as immunodominant B-cell epitopes in w

57 n of stem cell factor dimer, a total of five deamidated forms, including two homodimers and three het

58 or the initiation of celiac disease in which deamidated free human peptides with T-cell epitope homol

59 ELISA, all the sera displayed IgE binding to deamidated gamma- and omega2-gliadins and deamidated tot

60 TG and total IgA, or IgA-TTG and IgG against deamidated gliadin (IgG-DGL) could identify patients wit

61 tests against tissue transglutaminase (TG2), deamidated gliadin peptide (DGP) and endomysium (EMA).

62 subset of study participants, mean levels of deamidated gliadin peptide autoantibodies were 7.46 (6.9

63 bodies against tissue transglutaminase-2 and deamidated gliadin peptide), symptom frequencies, and sa

64 tibodies against tissue transglutaminase and deamidated gliadin peptide, greatly facilitate diagnosis

65 The detection of IgG against deamidated gliadin peptides (DGP) has high specificity a

66 ased on the detection of serum antibodies to deamidated gliadin peptides (DGPs).

67 antibodies against native gliadin (ngli) and deamidated gliadin peptides (dpgli), as well as for IgA

68 Novel deamidated gliadin peptides antibodies have better diagn

69 utamic acid residues of the TG2 epitope with deamidated gliadin peptides could be a structural basis.

70 or TGA-IgA, 1 for TGA-IgG, 6 for IgG against deamidated gliadin peptides, and 1 for EMA, from 5 diffe

71 c antigens tissue transglutaminase (tTG) and deamidated gliadin, and the classifier accuracy was inde

72 lmark antigens tissue transglutaminase-2 and deamidated gliadin, exhibiting 71% sensitivity and 99% s

73 mysium, as well as IgA and IgG antibodies to deamidated gliadine peptide and TG6 and performed HLA an

74 mmunofluorescence assay, and antibodies to a deamidated gliadine peptide using an immunofluorometry a

75 ve to IgA antibodies to TG2, endomysium, and deamidated gliadine peptide.

76 It can be evidenced by strong IgE binding to deamidated gliadins or peptides of the type QPEEPFPE.

77 By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bact

78 glutamine-50, and glutamine-147 residues, or deamidated glutamic acid residues at the same positions.

79 the consumption of food products containing deamidated gluten (DG) in subjects tolerant to wheat.

80 characterized by CD4(+) T cells recognizing deamidated gluten and by antibodies reactive to gluten o

81 ted from cultures with reactivity to complex deamidated gluten antigen or by sorting with gluten pept

82 , whereas T cells from adults only recognize deamidated gluten peptides.

83 tive and heteroclitic (stronger) response to deamidated gluten peptides.

84 Levels of T-cell responses were higher to deamidated gluten than to native gluten in children and

85 -adenosylhomocysteine, increases the rate of deamidated HA-CaM degradation.

86 The experiments indicate that deamidated HA-CaM is degraded after microinjection, whil

87 Enzymatic methylation of deamidated HA-CaM with purified PIMT prior to injection

88 An asparagine known to be deamidated in human cataract is located in a highly orde

89 ase A, and a tryptic peptide from calmodulin deamidated in its native state.

90 Total spore core protein also deamidated in vivo, although the rate was two- to threef

91 phaAN101D-transgene and is considered to be "deamidated" in this study.

92 dation accelerates amyloid formation and the deamidated material is able to seed amyloid formation by

93 se CheD deamidates Q609 in McpC and does not deamidate McpB.

94 owing posttranslationally phosphorylated and deamidated, modified sites in the bovine MBP components

95 A selectively deamidated multivalent peptide from gluten (LQLQPFPQPELP

96 inantly expressed wild-type betaB1 (WT), the deamidated mutant (Q204E), an N-terminally truncated mut

97 llins, and (iii) WT-alphaA:individual alphaB-deamidated mutant crystallins.

98 on deletion of the N-terminal domain in the deamidated mutant proteins, but opposite effects were ob

99 Our results indicated that the deamidated mutant was significantly destabilized relativ

100 These structural changes in the deamidated mutants led to decreased stability during unf

101 ected mutagenesis was used to generate three deamidated mutants of alphaB-crystallin: N78D, N146D, an

102 ompared with the WT-alphaA, the three alphaA-deamidated mutants showed reduced levels of chaperone ac

103 T-alphaB crystallins, (ii) individual alphaA-deamidated mutants:WT-alphaB crystallins, and (iii) WT-a

104 In addition, we present evidence that CheD deamidates other B. subtilis chemoreceptors including Mc

105 -acid peptide corresponding to the partially deamidated peptide of A-gliadin amino acids 57-73 (Q65E)

106 structs containing cross-linked tTG and Glia deamidated peptides have been synthesized.

107 epitopes and many have higher reactivity to deamidated peptides.

108 residues 140-173) deleted alphaA mutants and deamidated plus N-terminal domain or C-terminal extensio

109 w that fibers formed by samples that contain deamidated polypeptide contain reduced amounts of beta-s

110 sensitive signature peptide (IYPTNGYTR), its deamidated products (IYPTDGYTR and IYPTisoDGYTR), and a

111 1D patient could only be generated against a deamidated proinsulin peptide, but cross-reacted with na

112 cable to defining the isomerization state of deamidated proteins.

113 cts even for those peptides showing multiple deamidated proteoforms.

114 e between the chemoreceptors is because CheD deamidates Q609 in McpC and does not deamidate McpB.

115 ponses, tissue transglutaminase specifically deamidated Q65 in the peptide of A-gliadin amino acids 5

116 A gamma-type SASP of B. subtilis also deamidated readily in vitro (t(1/2) for one net deamidat

117 CheD not only deamidates receptor glutamine residues contained within

118 The characterization of Asn deamidated residues by LERLIC-MS/MS also uncovered novel

119 oop deletions or wild-type CNF1-C is able to deamidate RhoA containing Asn-63 instead of Gln-63, sugg

120 quired for enzymatic activity of the toxins, deamidates RhoA and Cdc42 better than CNF2.

121 hrome c, a tryptic peptide from unfolded and deamidated ribonuclease A, and a tryptic peptide from ca

122 arboxyl-terminal fragment were sufficient to deamidate RIG-I in vitro.

123 Purified PFAS and viral homolog thereof deamidate RIG-I in vitro.

124 cotinate was depleted and metabolites of the deamidated salvage pathway were reduced but intracellula

125 approximately 1 h at 70 degrees C), and the deamidated SASP no longer bound to DNA effectively.

126 the CheC/CheD/CheYp adaptation system and to deamidate selected residues to activate the chemorecepto

127 ned positions into gluten T-cell epitopes by deamidating specific glutamine residues on the basis of

128 midation, the sequence Asn(45)-Gly(46) being deamidated spontaneously at near-neutral and basic pH an

129 d WT-alphaB, the heteroaggregates containing deamidated subunits of either alphaA- or alphaB-crystall

130 en when deamidated, but V96F substitution of deamidated TFIIA amino acid residues 91-100 stimulated I

131 r was much less efficiently demethylated and deamidated than intact receptor, but essentially was unp

132 iting factors (Cifs) into mammalian cells to deamidate the ubiquitin-like protein NEDD8.

133 Dop (deamidase of Pup; Mtb Rv2112c/MT2172) deamidates the C-terminal glutamine of Pup to glutamate,

134 ues in Cu, Zn superoxide dismutase (SOD1) to deamidate to aspartate remains uncharacterized; its occu

135 a variant form of beta(2)M in which Asn17 is deamidated to Asp (N17D).

136 pectrometry demonstrated that ~23% of N26 is deamidated to aspartate (iso-aspartate was undetectable)

137 ealed that a single asparagine, Asn-164, was deamidated to aspartic acid in the pI 8.3 isoform.

138 thesized with a terminal glutamine, which is deamidated to glutamate by Dop (deamidase of Pup) prior

139 NMN produced by either route 2 or route 3 is deamidated to nicotinic acid mononucleotide and converte

140 ing residues, in that they are enzymatically deamidated to yield secondary destabilizing residues asp

141 to deamidated gamma- and omega2-gliadins and deamidated total gliadins, frequently with high concentr

142 artyl detection in peptides from proteins; a deamidated tryptic peptide of cytochrome c, a tryptic pe

143 observed that the exposed (568)NGR site can deamidate under conditions mimicking accelerated Asn agi

144 in the helicase 2i domain of RIG-I that were deamidated upon UL37 expression or HSV-1 infection.

145 volved in the first step of the two-step non-deamidating utilization of nicotinamide (NMN shunt).

146 trate for the carboxylmethyltransferase, but deamidated variants of calmodulin act as substrates for

147 D4/DQ8 mice also responded well to modified (deamidated) versions of the gliadin peptides, whereas HC

148 that confer loss of function phenotypes when deamidated via site-specific mutation.

149 it is bound to DNA, the alpha/beta-type SASP deamidated with a t(1/2) of 2 to 3 h at 95 degrees C.