ライフサイエンスコーパス: debranching

1 phosphate dissociation, ADF/cofilin promotes debranching.

2 the release of free glucose through glycogen debranching.

3 hat GMF depends on two separate surfaces for debranching.

4 2/3 complex at branch junctions and promotes debranching.

5 ddressed which factors promote actin network debranching.

6 requires manganese as the metal cofactor for debranching.

7 tween the roles of DBR1 in transposition and debranching.

8 loidin, to stabilize actin filaments against debranching.

9 of 70 patients (24%): chimney, 3; open iliac debranching, 1; coiling, 8; onyx, 3; and chimney plus on

10 Finally, we show that this debranching activity and mechanism are conserved for mam

11 e branchpoint junction were shown to inhibit debranching activity and, hence, represent "decoys" for

12 3 to alanine abolishes or greatly diminishes debranching activity in vitro.

13 SI N-terminal subunit has an additional exo-debranching activity on the alpha-1,6-linkage.

14 n accumulation when the only isoamylase-type debranching activity present is ISA1 homomer, but not in

15 a human data set acquired in the presence of debranching activity, LaSSO identified both canonical an

16 e investigated which enzymes are involved in debranching amylopectin during transient starch degradat

17 ts of Northern, ribozyme, RT-PCR, and lariat debranching analyses indicate that the two species are c

18 amma phosphate, ADF/cofilin proteins promote debranching and depolymerization.

19 GMF is implicated in both Arp2/3 debranching and inhibition of Arp2/3 activation.

20 cal feature of dendritic nucleation in which debranching and subsequent actin-filament remodelling an

21 entity of the molecular mechanism underlying debranching and whether this activity extends to mammali

22 prevented annealing of short filaments after debranching and, with profilin, allowed filaments to dep

23 at alpha-glucosidase activity, i.e. glycogen debranching and/or lysosomal glycogen breakdown, contrib

24 d into pre-miRNA hairpins after splicing and debranching, and miRNAs can also be excised by Dicer cle

25 iogenesis pathway involving splicing, lariat debranching, and RNA exosome-mediated "trimming," follow

26 Debranching appears to be a rate-limiting step for the t

27 Network disassembly and debranching appears to be linked to actin-bound ATP hydr

28 sing oligomers, indicating that severing and debranching are important steps in the disassembly proce

29 diester bonds was verified using an in vitro debranching assay.

30 op structure that is hypothesized to prevent debranching by cellular enzymes.

31 These data suggest that debranching by GMFbeta plays an important role in branch

32 that the lariat-intron is not accessible to debranching by purified Dbr1 when it is held in the T123

33 RS3 yields can be substantially enhanced by debranching cassava starch using pullulanase followed by

34 When the T7 gene 3-encoded DNA debranching endonuclease is absent during in vitro T7 DN

35 ensive network forms when the gene 3-encoded debranching endonuclease is present.

36 unction-specific resolvase can evolve into a debranching endonuclease tailored to the requirements of

37 of BE together with the starch synthases and debranching enyzmes were able to create crystallization-

38 D-III) is caused by a deficiency of glycogen debranching enzyme (AGL) activity.

39 investigations of group II intron splicing, debranching enzyme (Dbr) activity, and other biochemical

40 spliceosome must be hydrolyzed by the intron debranching enzyme (Dbr1) before they can be metabolized

41 tudies in our laboratory showed that the RNA debranching enzyme (DBR1) is not required for early step

42 rpin RNA (shRNA) knockdown of the RNA lariat debranching enzyme (DBR1) led to a decrease in the produ

43 ive disease, is caused by deficient glycogen debranching enzyme (GDE) activity.

44 Glycogen debranching enzyme (gene symbol, AGL) is a multifunction

45 Here, we report that the human RNA debranching enzyme (hDBR1), when inappropriately regulat

46 Functions of isoamylase-type starch-debranching enzyme (ISA) proteins and complexes in maize

47 ubunit of heteromeric isoamylase-type starch-debranching enzyme (ISA1/ISA2 heteromer).

48 tein body and found to be enriched in starch debranching enzyme (pullulanase).

49 been named AtDBR1 (for Arabidopsis thaliana Debranching enzyme 1).

50 pes of alpha(1-->6) glucan hydrolase (starch-debranching enzyme [DBE]).

51 n accumulation when the only isoamylase-type debranching enzyme activity present is ISA1/ISA heterome

52 Although the ex vivo activities of debranching enzyme and lysosomal acid maltase, two major

53 osin binding protein C (C-protein), glycogen debranching enzyme and ryanodine receptor 2 were also id

54 These data indicate that isoamylase-type debranching enzyme and SSIII work in a coordinated fashi

55 structure of the complex between the starch debranching enzyme barley limit dextrinase (LD), hydroly

56 t an enzyme of analogous nature to the plant debranching enzyme but of a different bacterial origin w

57 Saccharomyces cerevisiae, the absence of the debranching enzyme causes these lariat RNAs to accumulat

58 he 2',5'-bonds must be hydrolyzed by the RNA debranching enzyme Dbr1 before spliced introns can be de

59 A new study shows that loss of the lariat debranching enzyme Dbr1 suppresses TDP-43 toxicity.

60 Using fission yeast cells lacking the debranching enzyme Dbr1, LaSSO not only accurately ident

61 We now report the implication of starch debranching enzyme in the aggregation of semicrystalline

62 Lariat debranching enzyme is also necessary for siRNA productio

63 Because the debranching enzyme is conserved among eukaryotes, this a

64 indirect evidence that the heteromultimeric debranching enzyme ISA1-ISA2 is not involved in starch b

65 SS2, and SS3 (to vary chain lengths) and the debranching enzyme ISOAMYLASE1-ISOAMYLASE2 (ISA; to alte

66 genes on SBEIIa, starch synthase, or starch-debranching enzyme isoforms were observed.

67 This involved the recruitment of a debranching enzyme of chlamydial pathogen origin.

68 This enzyme was identified as a debranching enzyme of the isoamylase type.

69 that gene expression of LIMIT DEXTRINASE1, a debranching enzyme that cleaves branch points within sta

70 yces cerevisiae Dbr1 is a 405-amino acid RNA debranching enzyme that cleaves the 2'-5' phosphodiester

71 ells lacking both SpPrp18 and SpDbr1 (lariat debranching enzyme), a genetic background suitable for d

72 haracterized by a deficiency in the glycogen debranching enzyme, amylo-1,6-glucosidase,4-alpha-glucan

73 haracterized by a deficiency in the glycogen debranching enzyme, amylo-1,6-glucosidase,4-alpha-glucan

74 G (type 1 phosphatase-targeting subunit), or debranching enzyme, making it unlikely that these protei

75 -fold increase in the activity of the starch debranching enzyme, pullulanase (limit dextrinase), the

76 I, SBEIIb, and sugary1, the putative starch-debranching enzyme, were each highly enriched in the amy

77 The csrA gene did not affect glycogen debranching enzyme, which is now shown to be encoded by

78 action of the splicing machinery and lariat-debranching enzyme, which yield pre-miRNA-like hairpins.

79 cleavage of the 2',5' linkage by recombinant debranching enzyme.

80 eletion of DBR1, which encodes an RNA lariat debranching enzyme.

81 or to genes up-regulated by deletion of the debranching enzyme.

82 Pullulanase is a well-known starch-debranching enzyme.

83 tered by mutations of isoamylase-type starch-debranching enzymes (DBE), although how these proteins a

84 ific isoamylase- and pullulanase-type starch-debranching enzymes (DBEs) present in developing maize (

85 Isoamylase-type starch debranching enzymes (ISA) play important roles in starch

86 Conserved isoamylase-type starch debranching enzymes (ISAs), including the catalytic ISA1

87 e to alpha-(1-->6) glucan hydrolases (starch-debranching enzymes [DBEs]).

88 tide diversities, while pyrophosphatases and debranching enzymes are most conserved.

89 ity is that specific SSs, BEs, and/or starch debranching enzymes associate physically with each other

90 eals from a need for effective inhibition of debranching enzymes having characteristic open active si

91 r abundance of oligosaccharide degrading and debranching enzymes in buffalo rumen metagenome and that

92 our goal was to evaluate the role of starch debranching enzymes in the determination of the structur

93 The starch debranching enzymes isoamylase 1 and 2 (ISA1 and ISA2) a

94 h/glycogen synthases, branching enzymes, and debranching enzymes) are differentially expressed in Mul

95 zyme families (oligosaccharide degrading and debranching enzymes) in digestion of coarse feed.

96 ene with sequence similarity to higher plant debranching enzymes, and both mutants lacked a chloropla

97 Previous studies identified two debranching enzymes, isoamylase 3 (ISA3) and limit dextr

98 rotein with domains characteristic of lariat debranching enzymes, which has been named AtDBR1 (for Ar

99 pyrophosphorylase, and starch branching and debranching enzymes.

100 , starch branching enzymes (BEs), and starch debranching enzymes; however, the molecular explanation

101 and provide a molecular understanding of the debranching events associated with optimal starch mobili

102 Arp2/3 complex inhibitor and actin filament debranching factor, regulates lamellipodial protrusion d

103 d dbr1 mutant alleles are also deficient for debranching, further supporting a role for 2'-5' phospho

104 When debranching is blocked, these splicing intermediates can

105 Effective debranching is nevertheless achieved, as a result of coo

106 ester bond found in excised intron lariats ("debranching") is essential for turnover of intronic sequ

107 by a novel mechanism and that branching and debranching may play roles in Ty1 reverse transcription

108 ison of tiling array signals of RNA from the debranching mutant to the wild-type parent strain, and t

109 along with mutagenesis studies, suggest that debranching (not inhibition of Arp2/3 activation) is a p

110 we demonstrated that GMF potently stimulates debranching of actin filaments produced by Arp2/3 comple

111 One of the only factors known to promote debranching of actin networks is the yeast homolog of gl

112 plex plays a physiological role by promoting debranching of aged branch junctions without interfering

113 The results reveal the high debranching of arabinan side chains of RG I as compared

114 Strikingly, cortactin potently inhibited the debranching of filament networks.

115 h can arise by either hydrolytic splicing or debranching of lariat RNA, cannot carry out both reverse

116 Debranching of OS starches was incomplete compared with

117 ge (canonical substrates) or by splicing and debranching of short introns (mirtrons).

118 ackaging is thought to be dependent upon DNA debranching or other repair processes, and such events c

119 with a need for terminal repeat duplication, debranching, or damage repair concomitant with DNA packa

120 tin chains at similar rates and with similar debranching patterns, producing monoubiquitin species.

121 TP state, and nucleotide hydrolysis promotes debranching, suggesting that the higher affinity of GMF

122 Debranching this RNA in vitro with Dbr1p creates an unca

123 ma-phosphate from ADP-Pi-actin filaments and debranching to 30 seconds.