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1 ependent Escherichia coli cells growing in a defined medium.
2 did not affect the growth rate in amino acid-defined medium.
3 were overlain with collagen in a serum-free defined medium.
4 rnQ1 during S. aureus growth in a chemically defined medium.
5 spase-9 activation when cells were placed in defined medium.
6 Salmonella enterica serovar Typhimurium on a defined medium.
7 entiated into oligodendrocytes in serum-free defined medium.
8 elop in rat neural crest cultures grown in a defined medium.
9 asitone-based complex medium or a chemically defined medium.
10 rg, Gln/Glu, His, Leu, and Trp in chemically defined medium.
11 zyme activity than cells from the chemically defined medium.
12 ains incubated in an insulin free/serum free defined medium.
13 vive--when plated in mitogen-free chemically defined medium.
14 eta2 or TGFbeta3 was added to the chemically defined medium.
15 ight junctions when maintained in hormonally defined medium.
16 ggered apoptosis in high serum as well as in defined medium.
17 ic cell death, which occurs spontaneously in defined medium.
18 d effects were more striking in a chemically defined medium.
19 Surface, for culture of hMSCs in serum-free, defined medium.
20 oints were cultured in serum-free chemically defined medium.
21 ile l-alanine-induced spore germination in a defined medium.
22 tured as monolayers in serum-free chemically defined medium.
23 a gel-based extracellular matrix (ECM) and a defined medium.
24 with continuous flowthrough of a dynamically defined medium.
25 orrhoeae in response to iron availability in defined medium.
26 layer over 72 h in control cultures grown in defined medium.
27 d ouabain resistant) feeders in a serum-free defined medium.
30 n of the initial SC population in chemically defined medium; (3) a magnetic-activated cell sorting pu
31 the enzyme copy numbers in cells cultured in defined medium (3517 +/- 1578) and in complex medium (47
32 plemented with excess glucose and chemically defined medium allowed survival for less than 1 week.
33 ing growth factor-beta 1 (TGF-beta 1), or in defined medium alone showed no morphological or biochemi
34 BCG mutants grow only on lysine-supplemented defined medium and are unable to form colonies on comple
35 nt was auxotrophic for purines when grown in defined medium and exhibited significant attenuation in
36 roth medium but strongly inhibited growth in defined medium and host cells and impaired the generatio
37 acellular matrix (ECM) coatings in xeno-free defined medium and in traditional conditions on tissue c
38 sses transcription of PTS components in both defined medium and LB broth and that E. coli Mlc is able
39 ns were able to form robust biofilms both in defined medium and on tomato plant roots and exhibited s
40 N: 180 and 400 mg N/l) in a simil grape must defined medium and two S. cerevisiae strains commonly us
41 r >150 population doublings in a serum-free, defined medium and with a doubling time of approximately
42 NCAM immunoreactive, undergo self-renewal in defined medium, and differentiate into multiple neuronal
43 Compared to in vitro biofilm growth on a defined medium, approximately 14% of the A. actinomycete
44 hyl sulfoxide (DMSO)-supplemented chemically defined medium are an excellent model system for studyin
45 ylsulfoxide (DMSO) culture, fed a chemically defined medium, are highly differentiated and an excelle
46 o growth defect during in vitro culture in a defined medium at 37 degrees C and appeared to have norm
48 grown in continuous culture in a chemically defined medium at fast mass doubling time (t(d) = 1.8 h)
49 ns group streptococci, growing in chemically defined medium at pH 6.8, released HlpA into the milieu
51 rium mineralized all three models rapidly in defined medium, but C. subvermispora showed appreciable
52 have been reported to lack LiP when grown on defined medium, but it is not clear whether they exhibit
53 rat embryonic stem cells can be sustained in defined medium by dual inhibition (2i) of the mitogen-ac
54 Klebsiella pneumoniae capsule expression in defined medium by nearly 90%, which exposed subsurface s
55 arative transcriptomics of mycelium grown on defined medium, casing-soil, and compost revealed genes
56 oteases were recovered from spent chemically defined medium (CDM) and fractionated by ammonium sulfat
58 of competence was examined in the chemically defined medium (CDM) described by van de Rijn and Kessle
60 The mtaR mutant grew poorly in chemically defined medium (CDM) prepared with methionine at a conce
61 aerobically grown in a low-iron, chemically defined medium (CDM), L. pneumophila secretes a substanc
62 develop in silico and in vitro a chemically defined medium (CDM), which was validated experimentally
64 uced approximately in half in the chemically defined medium compared with the same medium supplemente
68 differentiation strategy, using a chemically defined medium consisting of just three components: the
69 C2C12 mouse myoblasts were cultured in a defined medium containing 0 or 70 micromol choline/L for
70 c abnormalities whereas growth in chemically defined medium containing 5 mM KCl more closely resemble
71 agar, and only poor growth was obtained in a defined medium containing albumin as the sole protein so
73 We describe a reproducible protocol, using defined medium containing bone morphogenetic protein 4 b
74 The medium was exchanged for a serum-free defined medium containing corresponding steroids +/- tum
75 ed cells could not proliferate in chemically defined medium containing CSF-1 and continued to require
76 ococcus neoformans NIH 409 was cultured in a defined medium containing D-[1-13C]xylose (Xyl), D-[1-13
77 protein substrates (collagen or laminin) and defined medium containing epidermal growth factor and ni
79 ased growth of H. influenzae in a chemically defined medium containing Neu5Ac, supporting an addition
80 Under more physiological conditions, in a defined medium containing purified LPS-binding protein,
81 sendoderm reporter MIXL1-GFP in a chemically defined medium containing the canonical WNT signaling ac
82 enatal donor eyes and cultured in serum-free defined medium containing the commercially formulated su
83 rved when microbes are grown in a chemically defined medium containing two sugars (for example glucos
84 taneously gave rise to differentiated OLs in defined medium, cultures of multipotent progenitors isol
85 erozygous female mice with preCGG repeats in defined medium display shorter dendritic lengths and few
86 osphate defined medium or in a low-phosphate defined medium during exponential growth, a growth condi
87 ability of the mutant strain to acidify the defined medium during growth in the presence of glucose
88 When adult SCG explants were cultured in defined medium, endogenous VIP immunoreactivity was rele
90 howed that H. pylori grows in the chemically defined medium F-12, but not in other tissue culture med
91 lso show that reprogramming using chemically defined medium favors formation of fully reprogrammed ov
93 o-cultured in vitro in serum-free chemically defined medium, functional anchoring junctions such as c
96 in combination with a serum-free, hormonally defined medium (HDM) tailored for the adult cell type of
97 nd control rats were treated with hormonally defined medium (HDM), base medium conditioned by RCS or
101 retina from newborn rabbits were cultured in defined medium in the absence of serum or soluble growth
103 rains were cultured in Ham's F-12 chemically defined medium in the presence or absence of cholesterol
104 , and zinc to the DMSO-containing chemically defined medium induced DNA synthesis and cell replicatio
106 The fucose phenotype, shown in chemically defined medium, is strain specific and linked to an 11-o
110 as significantly decreased when a chemically defined medium lacking N-acetylneuraminic acid (sialic a
112 n in the complex laboratory medium PYE or in defined medium (M2G) supplemented with casamino acids or
113 TVKHRPDALHPQ or LTTAPKLPKVTR in a chemically defined medium (mTeSR), they expressed markers of plurip
116 successful field test, to the cultivation in defined medium of the organisms responsible for extensiv
118 y addition of sigX-inducing peptide (XIP) in defined medium or by competence-stimulating peptide (CSP
119 matis mutant can grow on lysine-supplemented defined medium or complex rich medium, while the BCG mut
120 either throughout growth in a high-phosphate defined medium or in a low-phosphate defined medium duri
121 Lens epithelial explants were cultured in defined medium or medium supplemented with FGFs or vitre
123 Growth of the yfu mutants in a deferrated, defined medium (PMH2) at 26 and 37 degrees C failed to i
124 he addition of pectin and glucan to a simple defined medium resulted in dramatic changes in X. fastid
125 at hemoglobin supplemented into a chemically defined medium significantly and specifically induced fi
126 d biological activity were spiked into (1) a defined medium simulating the composition of human vagin
127 sis induction medium, but also in chemically defined medium specifically for osteogenesis, and concur
130 il collagen coated dish and fed a chemically-defined medium supplemented with 2% dimethylsulfoxide (D
131 ng after bacterial cultivation in chemically defined medium supplemented with [U-(13)C(6)]- or [1,2-(
132 tes biofilm formation in LB broth but not in defined medium supplemented with either pyruvate or gluc
133 previously described the use of a chemically defined medium supplemented with epidermal growth factor
135 cer of FN receptor expression and describe a defined medium supplemented with hemoglobin that greatly
136 multocida was grown in iron-free chemically defined medium supplemented with hemoglobin, transferrin
137 , cells of L. casei ATCC 334 were grown in a defined medium supplemented with various sugars, includi
138 moter, we found that a serum-free chemically defined medium supports formation of embryoid bodies (EB
140 ne auxotroph requires 25-fold more lysine on defined medium than do the other mutants and is dependen
141 Because RPMI is a more stable and chemically defined medium than M3, the determination at 24 h of the
142 CBPs were derived by adapting bacteria to a defined medium that substituted ethanolamine for choline
143 role for these proteins, a simple chemically defined medium that supports growth of adult Drosophila
145 ntricular myocytes maintained in serum-free, defined medium that were continually paced at 3 Hz for u
147 When control hESCs were grown in chemically defined medium, their expression of markers of pluripote
149 nal cords by immunoselection and cultured in defined medium, they all differentiated into oligodendro
150 glycosaminoglycans and could be used with a defined medium to culture hES cells for more than 3 mont
151 )O(2)) at multiple time points in a nutrient defined medium to identify a transcriptional signature a
152 the wild type when the strains are grown in defined medium unless additional serine is provided, sug
153 mplex medium, but did not grow in chemically defined medium unless supplemented with the metals coppe
154 d failed to grow aerobically in a chemically defined medium, unless supplemented with acetate which p
155 Addition of single amino acids to vaginal defined medium (VDM) was stimulatory to the growth of P.
156 unction mutant strain grown in low-phosphate defined medium was significantly higher than expression
159 oli K-12 MG1655 during exponential growth in defined medium, we separated soluble proteins of E. coli
160 ta pathway activity in a minimal, chemically defined medium, we show parallel, robust, and reproducib
161 ssessed by monitoring growth in a chemically defined medium where the only source of the essential am
162 urface ectoderm, in a serum-free, chemically defined medium with 10-50 ng/ml of FGF-2 induced gross c
163 reatment (2 mm) of cells containing no PUFA (defined medium with 18:0/stearic acid) produced 6.5 M1dG
164 the human stomach but does not survive in a defined medium with a pH <4.0 unless urea is present.
165 h conditions, being increased by growth on a defined medium with either L-lactate or pyruvate as the
166 ty, but the activity is induced by growth in defined medium with L-lactate as the carbon source.
167 n abolish Pseudomonas aeruginosa growth in a defined medium with malonate as the sole carbon source.S
169 with purified salivary amylase grew well in defined medium with potato starch as the sole carbohydra
171 crest from naive neural plates in vitro in a defined medium without added factors, whereas BMPs requi
174 ontinuously grown in a completely chemically defined medium without serum supplementation, it was pos
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