ライフサイエンスコーパス: degenerate primer

1 sequences will be amplified by a particular degenerate primer.

2 ponse genes, such as c- fos, using partially degenerate primers.

3 re based on multiplex PCR using consensus or degenerate primers.

4 m the VP7 gene segment using a pair of novel degenerate primers.

5 of AAV and adenovirus sequences by PCR using degenerate primers.

6 aZ1 and these genes were then used to design degenerate primers.

7 onnexins using reverse transcriptase-PCR and degenerate primers.

8 mens without the use of sequence-specific or degenerate primers.

9 was used to detect the virus using exact and degenerate primers.

10 erase chain reaction with specially designed degenerate primers.

11 u open reading frame) from C. burnetii using degenerate primers.

12 Pto-like sequences that were amplified with degenerate primers.

13 reaction (RT-PCR) with specifically designed degenerated primers.

14 Using degenerate primers, a cDNA clone encoding a putative mem

15 base scanning, and RT-PCR amplification with degenerated primers against transmembrane regions conser

16 The use of degenerate primers allows the simultaneous amplification

17 indodicarbocyanine-dCTP and another pair of degenerate primers also broadly specific for all genotyp

18 transcription-polymerase chain reaction with degenerate primers and by 5'- and 3'-rapid amplification

19 We constructed degenerate primers and conducted PCR analysis to identif

20 sing a PCR strategy based on short minimally degenerate primers and R. eglanteria (clearnose skate) s

21 ned by nested polymerase chain reaction with degenerate primers, and full-length ps20 cDNA clones wer

22 through gene cloning, PCR amplification with degenerate primers, and genome sequencing projects.

23 ranscription-polymerase chain reaction using degenerate primers, and the entire open reading frame wa

24 clones encoding this proteinase, we used two degenerate primer approaches to amplify part of the codi

25 is technique involves the use of a partially degenerate primer as a walker primer and a set of nested

26 f chemokine receptor messages by RT-PCR with degenerate primers at embryonic day 7.5 (E7.5) or by RNa

27 amplification of cDNA ends) strategy with a degenerate primer based on the conserved cytochrome P450

28 olymerase chain reaction amplification using degenerate primers based on a conserved region of previo

29 screening with a PCR product obtained using degenerate primers based on an amino acid sequence from

30 ied by polymerase chain reaction (PCR) using degenerate primers based on conserved regions of known p

31 Degenerate primers based on conserved sequences in these

32 erprints produced by PCR amplification using degenerate primers based on repetitive extragenic palind

33 cDNAs encoding LIP-36 have been cloned using degenerate primers based on the amino acid sequences.

34 A panel of degenerate primers based on the L1 (major capsid protein

35 Using degenerate primers based on the partial internal sequenc

36 dentify the schistosome co-Smad, we designed degenerate primers based on the sequence of the conserve

37 Genomic polymerase chain reaction using degenerate primers based on these exons led to two disti

38 Using degenerate primers based on these sequences, the polymer

39 Degenerate primers, based upon regions that are conserve

40 The use of degenerate primers combined with topoisomerase I treatme

41 own and novel genes in a family by using one degenerate primer complementary to a gene family-specifi

42 Polymerase chain reaction (PCR) using degenerate primers complementary to genes encoding d-Ala

43 eaction amplification of cDNA libraries with degenerate primers corresponding to conserved regions of

44 Degenerate primers corresponding to the codons derived f

45 Using degenerate primers corresponding to the highly conserved

46 Employing the degenerate primer-dependent polymerase chain reaction ap

47 ii WVU45 was initially amplified by PCR with degenerate primers derived from conserved amino acid seq

48 y other enteric bacteria; therefore, we used degenerate primers derived from conserved bacterial F1F0

49 efore, a duplex probe was PCR amplified with degenerate primers derived from internal sequences.

50 (RT)-PCR products were synthesized with two degenerate primers derived from the conserved motifs of

51 lic segment were isolated using PCR based on degenerate primers derived from the CYP52 family of alka

52 GBV-C RNA by polymerase chain reaction using degenerate primers derived from the NS3 helicase and 5'-

53 DNA by polymerase chain reaction (PCR) using degenerate primers derived from the sequences of two con

54 the user to quickly and effectively address degenerate primer design considerations.

55 OP intelligently guides the user through the degenerate primer design process including uploading seq

56 user can quickly scan over an entire set of degenerate primers designed by the program to assess the

57 By using degenerate primers designed from the partial amino acid

58 , obtained by PCR amplification of cDNA with degenerate primers designed using the amino acid sequenc

59 e conventional practice is to create several degenerate primers each of which variably encode the pep

60 Using PCR amplification with targeted and degenerate primers followed by cloning and sequencing, w

61 enes by Polymerase Chain Reaction (PCR) with degenerate primers followed by phage library screening.

62 n of the target region of the ent genes with degenerate primers, followed by characterization of the

63 16S rRNA gene polymerase chain reaction with degenerate primers, followed by high-throughput sequenci

64 Reverse transcriptase PCR (RT-PCR) using degenerate primers for both the rat liver Na+-dependent

65 ols exist that allow comprehensive design of degenerate primers for large groups of related targets b

66 duct with P. shumwayae DNA or cDNA, and (iv) degenerate primers for NRPS genes failed to amplify any

67 Degenerate primers for PCR, based on the VP14 predicted

68 leukemic cell lines were investigated using degenerate primers for reverse transcription-PCR followe

69 d using polymerase chain reaction (PCR) with degenerate primers for the active site of calpains.

70 We used degenerate primers for the amino- and carboxyl-terminal

71 SRB to arsenic biomethylation, and developed degenerate primers for the amplification of arsM genes t

72 polymerase chain reaction amplification with degenerate primers for the DUB-2 complementary DNA, 3 mu

73 cloned and sequenced using sequence specific degenerate primers from the seeds of Lablab purpureus co

74 d DNA polymerase (EC 2.7.7.7) and which uses degenerate primers in a nested format was developed.

75 Inosine-containing degenerate primers may be widely applicable to the ident

76 countered by (1) targeting loci with highly degenerate primers or conserved priming sites, (2) incre

77 mplification bias is reduced considerably by degenerate primers or targeting amplicons with conserved

78 PCR products (verifiable by sequencing) with degenerate primer pairs designed within the butyrate kin

79 t of algorithms designed to select and match degenerate primer pairs for the amplification of viral g

80 entification of the gerbil C5aR cDNA using a degenerate primer PCR cloning strategy.

81 ter for the kutzneride family, identified by degenerate primer PCR for halogenating enzymes postulate

82 Using degenerate primer polymerase chain reaction (PCR) on hum

83 lated from mouse embryonic kidney by using a degenerate primer polymerase chain reaction and cloning

84 utilized published peptide sequence data and degenerate primer polymerase chain reaction to clone two

85 d for >40 HPV types with use of MY09/MY11 L1 degenerate primer polymerase chain reaction-based method

86 ene was isolated from a cDNA library using a degenerate primer/polymerase chain reaction approach.

87 By using a PCR-based procedure with two degenerate primers representing sequences of these pepti

88 ll it the Markov Chain Monte Carlo Optimized Degenerate Primer Reuse (MCMC-ODPR) algorithm.

89 rformed with V. cholerae chromosomal DNA and degenerate primers, revealing six novel and distinct cod

90 We followed a degenerate-primer reverse transcriptase-PCR (RT-PCR) app

91 Through the use of a series of degenerate primers selected from consensus sequences of

92 acids was cloned from rice bean seeds using degenerate primer set.

93 onal reverse transcription-PCR (RT-PCR) with degenerate primer sets targeting conserved regions of th

94 PDX2 was isolated by a degenerate primer strategy based on conserved sequences

95 By using degenerate primers targeting a conserved region of a her

96 Since this method does not involve degenerate primers targeting HPV genomic regions, PCR bi

97 as a universal marker, we employed consensus-degenerate primers targeting the Proteobacteria.

98 nces (Sasa CaSR1-6), isolated using the same degenerate primers that amplified SVR sequences, cluster

99 polymerase chain reaction amplification with degenerate primers that amplified the conserved kinase d

100 ilized cultures are not toxic to fish, (iii) degenerate primers that amplify PKS genes from several p

101 that would implement primer reuse to design degenerate primers that could be designed around SNPs, t

102 guanylyl cyclase family as our templates and degenerate primers that discriminate this family from ot

103 nt to the 3'-end of the primer, allowing the degenerate primer to bind yet preventing extension by DN

104 uenced from a Z. rouxii cDNA library using a degenerate primer to the N-terminal sequence of the puri

105 olymerase chain reaction (PCR) approach with degenerate primers to amplify potential VAMP-like v-SNAR

106 nscription-PCR of Arabidopsis leaf RNA using degenerate primers to be an early nodulin (ENOD)-like pr

107 We used RT-PCR and degenerate primers to clone homologues of the tomato kin

108 s property is the basis for synthesizing the degenerate primers to clone several cDNAs of the ALDH is

109 ranscription polymerase chain reaction using degenerate primers to conserved amino acid sequences fro

110 PCR amplification using degenerate primers to conserved motifs of the predicted

111 Combinations of axonemal-specific and degenerate primers to conserved regions around the catal

112 mploying the polymerase chain reaction using degenerate primers to conserved regions of the sialylmot

113 us Neurospora crassa based on screening with degenerate primers to conserved regions of these signali

114 f a novel zinc finger protein, Zfp289, using degenerate primers to specifically amplify cDNAs from Id

115 Degenerate primers to two conserved regions of the Bcl-2

116 ines nested, insertion-specific primers with degenerate primers, to amplify DNA flanking the transpos

117 An HSP70 gene family-specific degenerate primer was used as a 5' primer in place of th

118 btain the corresponding cDNA clone, a set of degenerate primers was constructed based on consensus se

119 obtain a germacrene synthase cDNA, a set of degenerate primers was constructed based on conserved am

120 mooth muscle, reverse transcription-PCR with degenerate primers was performed on mouse intestine and

121 PCR using degenerate primers was used to clone genes from Streptom

122 Polymerase chain reaction using degenerate primers was used to identify genes encoding p

123 se chain reaction analysis, using a panel of degenerate primers, was negative for papilloma family vi

124 In PCRs with five different pairs of degenerate primers, we always obtained alpha1D products,

125 Using PCR amplification with degenerate primers, we identified and cloned a sigma fac

126 Using degenerate primers, we obtained a PCR fragment of the ch

127 ntified by database searches, plant-specific degenerate primers were derived and used to amplify a co

128 Degenerate primers were designed against these genes.

129 Degenerate primers were designed based upon an alignment

130 Consensus degenerate primers were designed from a conserved VP3 mo

131 Two specific degenerate primers were synthesized based on consensus s

132 herent weakness of this approach is that the degenerate primers will amplify previously identified, i