ライフサイエンスコーパス: deglycosylated

1 hERG protein accumulated in the cytosol was deglycosylated.

2 2D)beta2gamma2S receptors, endoglycosidase H deglycosylated 91 +/- 4% of alpha1(A322D) subunits, cons

3 In particular, ITs constructed with the deglycosylated A chain prepared from native ricin were t

4 together with estimation of the size of the deglycosylated A subunit relative to a series of C-termi

5 ase or decrease in C3b deposition on the two deglycosylated Abs was magnified when complement activat

6 irst glycosylated in the ER and subsequently deglycosylated after retrotranslocation into the cytosol

7 ition experiments conducted using native and deglycosylated aggrecan provided direct evidence for int

8 Apoptolidin H, the C27 deglycosylated analogue of apoptolidin A, displayed sub-

9 tructures of a PME from Aspergillus niger in deglycosylated and Asn-linked N-acetylglucosamine-stub f

10 vity for glucose oxidation compared with the deglycosylated and glycosylated (gPDH) forms of PDH.

11 dem mass spectrometry (LC/MS/MS) analysis of deglycosylated and intact glycopeptides from tryptic dig

12 e wild type US2 and US11 proteins as well as deglycosylated and polyubiquitinated class I degradation

13 a dimeric oxidized complex and then becomes deglycosylated and reduced to monomers.

14 d from the ER to the cytosol, where they are deglycosylated and subsequently degraded by the proteaso

15 sgenic mouse hearts produced both the 35-kDa deglycosylated and the 40-kDa glycosylated mobility form

16 s were observed with both intact gp120 and a deglycosylated and truncated form of gp120 protein that

17 ze the LC binding site on THP, human THP was deglycosylated and underwent limited trypsin digestion i

18 rst generated hypersialylated, desialylated, deglycosylated and untreated serum IgG and found that th

19 To prolong avidin clearance, avidin was deglycosylated and/or neutralized by acetylation of its

20 14 is endocytosed, extracted from membranes, deglycosylated, and degraded by proteasomes.

21 om the endoplasmic reticulum to the cytosol, deglycosylated, and degraded by the proteasome.

22 polonica converted stilbenes to ring-opened, deglycosylated, and dimeric products.

23 Our data for glycosylated, partially deglycosylated, and fully deglycosylated molecules furth

24 Ephrin-A1 was enzymatically deglycosylated, and its activity was evaluated in severa

25 SO-treated, and the protein was pulled down, deglycosylated, and resolved by electrophoresis.

26 ic mice receiving either anti-Abeta (2H6) or deglycosylated anti-Abeta (de-2H6) showed significant im

27 Deglycosylated anti-Abeta antibodies may be preferable t

28 Humanized, deglycosylated anti-HPA-1a mAbs may represent a novel tr

29 tilage (without additional purification), if deglycosylated appropriately, can be used as arthritogen

30 Previous studies suggested that deglycosylated beta1-LAP can not form this latent comple

31 s, and PS.beta2GPI binding experiments using deglycosylated beta2GPI, suggested that carbohydrate res

32 experiments conducted with an enzymatically deglycosylated Bla g 2 and a N268Q mutant showed that th

33 A deglycosylated breakdown intermediate of the heavy chain

34 In vitro phosphorylation of native and deglycosylated BSPs by casein kinase II identified seven

35 During this process, the Class I MHC HCs are deglycosylated by an N-glycanase-type activity.

36 lycans, or membrane-associated and partially deglycosylated by cytosolic N-glycanase.

37 rprised to find both molecules that had been deglycosylated by peptide N-glycanase and a large number

38 Glycopeptides thus selected were mixed, deglycosylated by PNGase F, and fractionated by reversed

39 N-deglycosylated C1INH also failed to suppress fluorescein

40 an enzyme linked immunosorbent assay, the N-deglycosylated C1INH bound to LPS very poorly.

41 veral in vitro and in vivo models, whereas N-deglycosylated C1INH loses such activities.

42 As with intact LPS, binding of N-deglycosylated C1INH to dLPA and mLPA was diminished in

43 We analyzed both native and enzymatically deglycosylated C4b2 and C2 and showed that the resulting

44 Purified deglycosylated CD147 failed to induce MMP-1 or MMP-2, bu

45 Difference maps between the glycosylated and deglycosylated CD155 complexes determined the sites of t

46 Both glycosylated and fully deglycosylated CD155 exhibited similar binding sites and

47 etry on purified and peptide N-glycosidase F-deglycosylated CD36 and also by comparing the electropho

48 enhanced binding; (c) O-linked glycans on N-deglycosylated CD44 enhanced binding; and (d) N-acetylga

49 Our data show that glycoforms of CD66e and deglycosylated CD66e are recognized by gonococci in an O

50 Analysis of deglycosylated cell lysates indicated that up to 40 kDa

51 g deglycosylation of cell membrane proteins (deglycosylated cells Bmax: 6.83 Hz; glycosylated cells B

52 Both the level and stability of cytosolic, deglycosylated CFTR are increased by proteasome blockade

53 (4 microM, 1 h) stabilizes the presence of a deglycosylated CFTR intermediate for up to 5 h without i

54 Deglycosylated CFTR is present under the same conditions

55 The mass differences between the deglycosylated chain a (17, 524.0) and a1 to a4 correspo

56 nt and the single-channel conductance of the deglycosylated channels was slightly reduced relative to

57 Deglycosylated channels were significantly more active (

58 spectral differences between the native and deglycosylated channels, thereby indicating that no net

59 differences in stability between native and deglycosylated channels.

60 olated, purified by gradient centrifugation, deglycosylated, characterized, and tested for arthritis

61 he 2.9-A-resolution structure of a partially deglycosylated complex of human FSH bound to the extrace

62 n at their predicted molecular masses in the deglycosylated complex.

63 the existence of several naturally occurring deglycosylated components of DLIF in human serum.

64 The presence of deglycosylated congeners of DLIF in human serum, includi

65 ortion of these molecules shows DLIF and its deglycosylated congeners to be 10(3)-fold less reactive

66 beled that molecule within its M(r) = 45,000 deglycosylated core.

67 roversial folded-back crystal structures for deglycosylated CR2 SCR 1-2.

68 he integrity of the protein, as truncated or deglycosylated Cripto-1 lacked BMP-4 binding activity.

69 in are upregulated after SCI and degrade the deglycosylated CSPG proteins.

70 Proteasome inhibitors can yield deglycosylated cytoplasmic intermediates that are the re

71 esized (AO)7 and anhydrous hydrogen fluoride-deglycosylated d(AO)51.

72 ) acceptor and a transgenically produced and deglycosylated d[AO](51) acceptor.

73 Oleuropein and its deglycosylated derivatives have high pharmaceutical inte

74 tion of the existence of naturally occurring deglycosylated derivatives of DLIF and establishes the l

75 subunit preparations and their alphaL2 loop-deglycosylated derivatives were combined with either eLH

76 to-apyrase, in contrast to the enzymatically deglycosylated ecto-apyrase and the tunicamycin-treated

77 c SIVmac239 and a live-attenuated, quintuple deglycosylated Env mutant (Delta5G) virus target CD4(+)

78 mented deglycosylated PDH is formed from the deglycosylated enzyme (dgPDH) when it spontaneously lose

79 ized glucose-oxidizing anode, prepared using deglycosylated enzyme coimmobilized with [Os(4,4'-dimeth

80 All the glycosylated and deglycosylated enzymes show direct electron transfer (DE

81 d by surface plasmon resonance confirms that deglycosylated ephrin-A1 does not bind EphA2 with high a

82 We found that deglycosylated ephrin-A1 does not efficiently induce Eph

83 doglycosidase-catalyzed glycosylation of the deglycosylated Fc domain (GlcNAc-Fc homodimer) with the

84 Similar studies of partially deglycosylated FH suggested that oligosaccharides were n

85 ndoplasmic reticulum and can be recovered as deglycosylated fluorescent intermediates in the cytosol.

86 Moreover, the deglycosylated form of both wild type and DeltaF508 CFTR

87 s in complex with an enzymatically partially deglycosylated form of the 3-domain ectodomain of Pvr at

88 tained from enzyme electrodes containing the deglycosylated form of the enzyme.

89 cells comigrates with both the native and a deglycosylated form of the radiolabeled receptor.

90 treated or pretreated with gp120 or with the deglycosylated form of this protein.

91 this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing four Asn-linked N

92 pastoris and at 1.40 A for the mutagenically deglycosylated form produced from Sf9 cells, show that t

93 The recombinant enzyme and a deglycosylated form, both expressed in Pichia pastoris,

94 injection of soluble egg Ag, but not by its deglycosylated form, suggesting a role for carbohydrates

95 itical since gangliosides are unable to bind deglycosylated forms of alpha(5)beta(1) from SCC12 and i

96 netic studies with native, desialylated, and deglycosylated forms of both enzymes indicate that the m

97 ity and recognized the glycosylated and both deglycosylated forms of gamma-glutamyl hydrolase.

98 zes the gI protein directly since completely deglycosylated forms of gI are efficiently recognized.

99 comparing exchange into the glycosylated and deglycosylated forms of the antibody.

100 tricted epitope, but not the glycosylated or deglycosylated forms.

101 insect cells) < pTF for the glycosylated and deglycosylated forms.

102 model predicts that FSHR binds Asnalpha(52)-deglycosylated FSH at a 3-fold higher capacity than full

103 rs, the binding of glycosylated FSH, but not deglycosylated FSH, would increase 3-fold, and that the

104 We also show that deglycosylated, full-length allergen is not activated ef

105 M) and that it did not bind to enzymatically deglycosylated gB2.

106 Deglycosylated Gc protein cannot be converted to macroph

107 Deglycosylated glycodelin was equipotent to intact glyco

108 ecific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and int

109 One PEG variant was created by modifying deglycosylated GO with short PEG chains (average of 350

110 udies have focused on the heavily truncated, deglycosylated gp120 core, leaving fundamental aspects o

111 lved structure of a mutant truncated, highly deglycosylated gp120 to propose a structural model for t

112 Pretreatment of CNS cultures with deglycosylated gp120, anti-T4A or anti-GC antibodies did

113 was displaced by unlabeled gp120 but not by deglycosylated gp120, suggesting that the binding was sp

114 the crystal structure of CD4 with truncated deglycosylated gp120.

115 omplexes were also produced of enzymatically deglycosylated gp160e and of gp160e variants with deleti

116 ered a strategic thrombin cleavage site into deglycosylated, HA-tagged human VMAT2.

117 - 2.0 Da) are isoforms of L1 (25, 837.5 in N-deglycosylated Hb) with glycan side-chains (GlcNAc)2 (Ma

118 with Au-hCG, LH receptors labeled with gold-deglycosylated hCG, an hCG antagonist, also exhibit rest

119 Recombinant and deglycosylated HCII bound heparin with dissociation cons

120 Binding of recombinant and deglycosylated HCII to DS, both with K(D) 4+/-1 microM,

121 for thrombin inactivation by recombinant and deglycosylated HCII were comparable, at optimal GAG conc

122 gG(1-4)), and recognize hIgG(1-4) as well as deglycosylated hIgG in western blotting assays.

123 46 kDa), similar to that of enzymatically N-deglycosylated hNET protein, which confirms the use of c

124 species, HO2-Co(III)-PEP (denoted CoPEP) and deglycosylated HO2-Co(III)-PEP (denoted CodPEP) have bee

125 we report the three-dimensional structure of deglycosylated, human erythrocyte AQP1, determined at 7

126 sent a three-dimensional (3D) density map of deglycosylated, human erythrocyte aquaporin 1 (AQP1) det

127 cR recognition, the receptor affinities of a deglycosylated IgG1 and its Fc fragment were determined

128 complex mixture from human plasma were fully deglycosylated in 20 min, without any apparent adverse a

129 om corresponding glycan oxazolines to the Fc-deglycosylated intact IgGs without product hydrolysis.

130 asome-inhibited US11 cells demonstrated that deglycosylated intermediates continued to be integrated

131 pecific immunoreactivity of glycosylated and deglycosylated LeAGP-1 with an antibody developed agains

132 Unexpectedly, these deglycosylated Lfs exhibited no loss in their ability to

133 dditionally, in cells, calreticulin binds to deglycosylated major histocompatibility complex class I

134 In addition, the deglycosylated MAL-bound proteins still interact with Om

135 N-linked carbohydrates and had an estimated deglycosylated mass of 64,000 Da.

136 correlated with enhanced calnexin binding to deglycosylated MHC class I heavy chains.

137 hogenic IgGs bound recombinant mouse MOG and deglycosylated MOG in myelin (tested by Western blot), b

138 sylated, partially deglycosylated, and fully deglycosylated molecules further revealed the criticalit

139 mulate in the cytosol as already reduced and deglycosylated molecules.

140 genic SIVmac239 virus and a live-attenuated, deglycosylated mutant Delta5G virus infected distinct CD

141 on their surfaces, the SF162-derived V2 loop-deglycosylated mutant viruses replicate as efficiently a

142 A deglycosylated mutant, beta(2-DeltaN), had no effect on

143 Molecular mass determinations of deglycosylated mZP2 and mZP3 suggest that their polypept

144 Both of the deglycosylated, nonreduced HA receptor proteins still bi

145 ha1beta2gamma2S receptors, endoglycosidase H deglycosylated only 26 +/- 5% of alpha1 subunits, consis

146 ntly inhibited if the antigen was chemically deglycosylated or if mannose receptors were blocked with

147 7 (77%) had IgE to both the glycosylated and deglycosylated Ory c 3 heterodimer.

148 Graphite electrodes modified with deglycosylated PcCDH (dPcCDH) and CsCDH (dCsCDH) have a

149 Fragmented deglycosylated PDH is formed from the deglycosylated enz

150 d except proline) motif were identified from deglycosylated peptide data as potentially atypical glyc

151 The deglycosylated peptide fractions were interrogated by ES

152 For grape invertase, in parallel with deglycosylated peptides analysis, different enzymatic di

153 y (MS)-based glycoproteomic approach wherein deglycosylated peptides are analysed by LC-MS/MS, while

154 ion that glycopeptides and the corresponding deglycosylated peptides share very similar MS/MS pattern

155 ternal standard technology, fractionation of deglycosylated peptides with reversed-phase chromatograp

156 alpha1-acid glycoprotein, the corresponding deglycosylated peptides, and the glycans released by the

157 ms of individual N-linked glycopeptides, the deglycosylated peptides, and the released, deutero-reduc

158 Dephosphorylation of deglycosylated PHF tangles results in increased tau rele

159 ed that recognition was mediated only by the deglycosylated phosphoantigen.

160 K-293/C-21 cells) was similar to that of the deglycosylated photo-cross-linked receptor (obtained by

161 tivities of recombinant HCII, and native and deglycosylated plasma HCII, and their affinities for hep

162 Peanut Ag (PNAg), but not deglycosylated PNAg, activated monocyte-derived dendriti

163 From these digests the completely deglycosylated polypeptide corresponding to RNase A in w

164 The crystal structure of deglycosylated pro-TPP1 was determined at 1.85 angstroms

165 of glycopeptides and glycoproteins and their deglycosylated products.

166 termined at 1.85 A for the endoglycosidase H-deglycosylated protease domain produced from P. pastoris

167 The expressed deglycosylated protein is the same size as SIRPalpha1, b

168 ha, the yield, purity and homogeneity of the deglycosylated protein resulting from this expression sy

169 N-Glycans, deglycosylated proteins, and intact glycoproteins from h

170 followed by separate analysis of glycans and deglycosylated proteins, which limits the ability to der

171 hout one of the carbohydrate chains, and the deglycosylated proteins-are detected by ProteinChip arra

172 tablished that mHR23B acts as a receptor for deglycosylated proteins.

173 o their enzymatic properties and mass of the deglycosylated proteins.

174 lar dynamics simulations of glycosylated and deglycosylated proteins.

175 n of calreticulin contributes to binding non/deglycosylated proteins.

176 ified PMCAb conditions that employ partially deglycosylated PrP(C) (dgPMCAb), an alternative transmis

177 mes and the sizes of the protease-resistant, deglycosylated PrP(Sc) fragments.

178 al rabbit antibodies were raised against the deglycosylated purified protein (dPELP) and purified ant

179 was expressed inPichia pastoris.Mo-MnLOX was deglycosylated, purified to homogeneity, and subjected t

180 The present study focuses on fragmented deglycosylated pyranose dehydrogenase (fdgPDH) from Agar

181 orylation, detected by the mobility shift of deglycosylated receptor, was unaffected by beta-arrestin

182 ple bands between 50 and 75 kDa, whereas the deglycosylated receptors migrated at 32 kDa, correspondi

183 HMW-BNP, when deglycosylated, was similar to deglycosylated recombinant proBNP.

184 With time, N-cadherin in clone-YH becomes deglycosylated, resistant to detergent extraction, and z

185 It allowed us to crystallize a fully deglycosylated rhodopsin (N2C/N15D/D282C).

186 IgG-HD37-SMPT-dgA is a deglycosylated ricin A chain (dgA)-containing immunotoxi

187 l antibody SN7 (IgG1) and its conjugate with deglycosylated ricin A chain (dgRA).

188 MoAb) has been linked chemically with either deglycosylated ricin A chain or truncated versions of Ps

189 ICs containing deglycosylated ricin A chain prepared from ricin toxin e

190 degraded to any significant extent; however, deglycosylated S(3)-RNase is degraded as efficiently as

191 The deglycosylated sample was further fractionated by revers

192 When the AGPs were partly or fully deglycosylated, SE-promoting activity was not diminished

193 lar dichroic spectra of the glycosylated and deglycosylated (Ser-Pro(2))(n), (Ser-Pro(3))(n), and (Se

194 -treated SP-A still enhanced uptake, whereas deglycosylated SP-A had significantly diminished ability

195 Furthermore, incubation of deglycosylated SP-A with M. tuberculosis failed to suppr

196 y recognizes LeAGP-1 in its glycosylated and deglycosylated states, and (4) immunolocalization of a s

197 ature TC II-R (62 kDa) and the enzymatically deglycosylated TC II-R (45-47 kDa) demonstrated optimal

198 de ((1)H(6)/(2)D(6)) reagents, enzymatically deglycosylated the differentially labeled peptides in th

199 were able to be reduced to a 46-kDa form and deglycosylated to a 38-kDa protein.

200 MS/MS experiment, so the tryptic digest was deglycosylated to confirm the presence of the glycopepti

201 inal subcellular compartments, where Nrf1 is deglycosylated to yield an active 95-kDa isoform.

202 The partially deglycosylated trimers are stable, and neither abnormall

203 on microscopic analysis shows that partially deglycosylated trimers have a structure similar to fully

204 Moreover, the deglycosylated trimers retain or increase their ability

205 Two unique deglycosylated tryptic peptides, (21)EAENITTGCAEHCSLNENI

206 lated and ubiquitinated species as well as a deglycosylated US11 intermediate.

207 y constant for the binding of neutravidin, a deglycosylated variant of avidin, to surface-immobilized

208 ino acid BNP standard, whereas HMW-BNP, when deglycosylated, was similar to deglycosylated recombinan

209 vative Hbs (reduced, carbamidomethylated and deglycosylated) were analysed by electrospray ionization

210 vative Hb (reduced, carbamidomethylated, and deglycosylated) were analyzed by electrospray ionization

211 egrees C for 90min and could then be further deglycosylated when mixed with almond or flax seed.

212 1 interacts with the O-GlcNAcase and becomes deglycosylated, which is likely to be important for expo

213 neously glycosylated IgG N-glycans are first deglycosylated with a wild-type endoglycosidase.

214 NPR-ECD deglycosylated with endoglycosidase F2 and endoglycosida

215 LAP produced in Chinese hamster ovary cells, deglycosylated with N-glycosidase F, forms a 80-90 kDa c

216 The purified enzyme could be deglycosylated with peptide N-glycosidase-F to a core mo

217 were differentially labeled, mixed, and then deglycosylated with the enzyme PNGase F.

218 esized protein into the cytosol, where it is deglycosylated, with conversion of the asparagines to as