ライフサイエンスコーパス: deglycosylation

1 (e.g., overnight enzymatic treatment for Fc deglycosylation).

2 migration in reducing gels before and after deglycosylation.

3 ng effector interactions of the antibody via deglycosylation.

4 version to a polyproline II-like helix after deglycosylation.

5 OF mass spectrometry after partial enzymatic deglycosylation.

6 However, LPS did not cause Sp1 deglycosylation.

7 Mr = 19,000 that shifted to Mr = 9,000 after deglycosylation.

8 ic population was promoted through enzymatic deglycosylation.

9 s demonstrated differential sensitivities to deglycosylation.

10 G-protein coupling of CCR5, or partial gp120 deglycosylation.

11 to a sharp band of approximately 20 kDa upon deglycosylation.

12 ivation by thrombin was not affected by mild deglycosylation.

13 vage of the rabbit AE2 Z-loop required prior deglycosylation.

14 ve for oxidation and disulfide exchange upon deglycosylation.

15 chymotrypsin, and papain, and to enzymatic N-deglycosylation.

16 n ring size and sensitivity to acid-promoted deglycosylation.

17 bstrate are required for subsequent antibody deglycosylation.

18 for which fluorescence depends on enzymatic deglycosylation.

19 ic proficiencies for pseudoglycosylation and deglycosylation.

20 -glycosylation site through PNGaseF-mediated deglycosylation.

21 hich are readily observed following N-linked deglycosylation.

22 und to have altered exchange properties upon deglycosylation.

23 mperature direct arylations, which minimizes deglycosylation.

24 Substrate hydrolysis was unaltered by deglycosylation.

25 n (1) migration on SDS-PAGE before and after deglycosylation, (2) the lack of a Kunitz-3 domain, and

26 Chemical deglycosylation abolished immunoreactivity with MAb 1B5

27 The various PrP species were resolved after deglycosylation according to their electrophoretic mobil

28 es suitable for transport and sequestration, deglycosylation activates them to carry out biological f

29 With this method, rutin-deglycosylation activity in buckwheat flour and a commer

30 A significant discrepancy in deglycosylation activity was observed between the ammoni

31 enzymes but most likely not by extracellular deglycosylation activity.

32 ctive biocatalysts with glycosylation and/or deglycosylation activity.

33 Because deglycosylation affects the function of many host protei

34 lycosylation plus dephosphorylation, but not deglycosylation alone, of AD P-tau and tau from PHF tang

35 of specific carbohydrate labelling, chemical deglycosylation and by introducing galE mutations; the l

36 suggesting that this complex couples protein deglycosylation and degradation.

37 performing enzymatic desialylation prior to deglycosylation and derivatization, leading to a more di

38 Moreover, using enzymatic deglycosylation and DTT derivatization combined with mas

39 arrive in the cytosol, where ubiquitination, deglycosylation and finally proteasomal proteolysis disp

40 markers of enveloped viruses using on-target deglycosylation and matrix-assisted laser desorption/ion

41 st clearance resulting from a combination of deglycosylation and neutralization.

42 Based on deglycosylation and nonreduced/reduced two-dimensional S

43 quired in the downstream events of substrate deglycosylation and proteasomal degradation.

44 slocation from the ER, followed by cytosolic deglycosylation and proteolysis by the proteasome.

45 ptide fragment of PrP(Sc) was found based on deglycosylation and PrP(Sc)-specific immunoprecipitation

46 N-Linked deglycosylation and reduction and alkylation of the 1-h

47 able triglycosides, thereby preventing their deglycosylation and release from tomato fruit upon tissu

48 ck rhodopsin maturation, thus inhibiting the deglycosylation and rhabdomeric targeting of newly synth

49 After deglycosylation and separation by SDS-PAGE, excised Coom

50 of envelope glycoprotein gp120 to enzymatic deglycosylation and the effects of enzyme treatment on i

51 modeling using the endoglycosidase-catalyzed deglycosylation and transglycosylation approach is emerg

52 mally ER-bound, and its release requires its deglycosylation and ubiquitination.

53 s of retrotranslocation, ubiquitination, and deglycosylation and, thereby, route misfolded glycoprote

54 on a combination of nonspecific proteolysis, deglycosylation, and matrix-assisted laser desorption/io

55 g to extensive proteolytic cleavage, partial deglycosylation, and protein-protein complex formation.

56 suggest that 1) partial immunopurification, deglycosylation, and SDS-PAGE separation of FPRs is suff

57 tination, retrotranslocation to the cytosol, deglycosylation, and targeting to the proteasome for deg

58 lycosylation pattern, their mobilities after deglycosylation are identical.

59 Glycosylation and deglycosylation are impressive mechanisms that allow pla

60 strong evidence substantiating glycosylation/deglycosylation as a likely self-resistance mechanism of

61 Deglycosylation assays and IgE inhibition tests confirme

62 Preliminary deglycosylation assays indicate that the epitope recogni

63 tibody access, proteinase K sensitivity, and deglycosylation assays.

64 ling; therefore, it could be speculated that deglycosylation at Asn(191) might be responsible for the

65 Molecular dynamics simulations indicate that deglycosylation at Asn-163 of CD16 removes the steric hi

66 dichroism spectroscopy showed that complete deglycosylation, but not the removal of peripheral sugar

67 Native virus deglycosylation by endo- and exoglycosidases dramaticall

68 e endoplasmic reticulum (ER), and subsequent deglycosylation by peptide-N-glycanase in the cytosol.

69 N-deglycosylation by peptide-N-glycosidase F digestion res

70 ial for optimal IL-13 inhibitory activity as deglycosylation by PNGase F showed lower activity.

71 y be coordinated with deamination by AID and deglycosylation by UNG2 to produce single-strand breaks

72 After enzymatic deglycosylation, channels were reconstituted into planar

73 on a combination of specific proteolysis and deglycosylation combined with two different mass spectro

74 ar weight proteins are degradation products; deglycosylation confirmed that the Mr 55, 000 sperm plas

75 EndoS deglycosylation converts pathogenic NMO-IgG autoantibodi

76 INTERPRETATION: EndoS deglycosylation converts pathogenic NMO-IgG autoantibodi

77 This was achieved by purification, deglycosylation, cyanogen bromide cleavage, and sequenci

78 might occur naturally within the cell due to deglycosylation/deamidation of this amino acid by the cy

79 ometry, antibody reactivity, and kinetics of deglycosylation, demonstrates that subtle structural and

80 Furthermore, although these deglycosylation-dependent fluorescent proteins are thems

81 Deglycosylation did not alter its electrophoretic mobili

82 olayer (SAM), in contrast to PcCDH for which deglycosylation did not exhibit any different electrocat

83 Digoxin is known to undergo deglycosylation during metabolism in humans.

84 ied in their stability and susceptibility to deglycosylation during thermal processing at pH 3, 5, or

85 the HDX-MS workflow with integrated PNGase A deglycosylation enables analysis of the native HDX of pr

86 Yeast peptide:N-glycanase (Png1p; PNGase), a deglycosylation enzyme involved in the proteasome depend

87 eras and analysed their glycan content using deglycosylation enzymes.

88 CC monoclonal antibodies in conjunction with deglycosylation experiments suggested that the 160- and

89 reonine or tyrosine; immunoprecipitation and deglycosylation experiments using anti-phosphotyrosine a

90 Deglycosylation experiments with three different glycosi

91 Unfortunately, inhibition of Fc function by deglycosylation failed to prevent this inflammatory resp

92 , which was reduced to 58,000 M(r) following deglycosylation, findings comparable with amino acid ana

93 inant and rat striatal receptors shifts upon deglycosylation from 43-48 to 42 kilodaltons.

94 a of the acid/base for the glycosylation and deglycosylation half-reactions.

95 e protease-resistant fragment of PrPSc after deglycosylation has a size of 19 kilodaltons, whereas th

96 Deglycosylation has been demonstrated to occur at the ER

97 The chemistry used by these enzymes for deglycosylation has been largely considered as the chemi

98 Using enzymatic deglycosylation, immunofluorescence, and quantitative ce

99 s indicated to be solely responsible for the deglycosylation in BER enzymes, however our results sugg

100 ration protocol involving microwave-assisted deglycosylation in conjunction with an automated sample

101 been associated with PKD, and we found that deglycosylation in cultured pancreatic beta cells altere

102 nts have suggested that such a glycosylation/deglycosylation is only a secondary self-defense mechani

103 PNGase A-based deglycosylation is thus performed after labeling (post-H

104 es involved in proteolysis and glycosylation/deglycosylation located in both compartments.

105 nging from yeast to mammals, suggesting that deglycosylation may play a central role in some cataboli

106 mouse meprin A using chemical and enzymatic deglycosylation methods and electrospray ionization mass

107 demonstrated by proteolytic peptide mapping, deglycosylation, micropurification, and Edman degradatio

108 gel at Mr = 19,000 that shifted to 10 after deglycosylation, most consistent with either of two glyc

109 ation by APC, factor V was subjected to mild deglycosylation (neuraminidase plus N-glycanase) under n

110 locyte FR-beta lacked mutations, and neither deglycosylation nor detergent solubilization restored fo

111 region is integrated in the ER membrane, and deglycosylation occurs before complete dislocation.

112 We then showed that transferrin deglycosylation occurs in vivo when B. fragilis is propa

113 f the activation parameters measured for the deglycosylation of 1 indicates that the half-life for de

114 ycosylation procedure that achieves complete deglycosylation of a diverse set of glycoproteins in app

115 These results demonstrate that deglycosylation of a human serum glycoconjugate(s) by th

116 ssing abasic sites generated by UDG-mediated deglycosylation of AID-effected dU, by extending DNA pas

117 Deglycosylation of an alpha-Gal-containing protein, bovi

118 Deglycosylation of antibodies abrogates the Fc-related e

119 that the loss of function is not because of deglycosylation of Asp-39 per se but rather is likely be

120 Deglycosylation of aspartoacylase or mutation at the gly

121 accessibility of HER2 to Herceptin following deglycosylation of cell membrane proteins (deglycosylate

122 expression in US2-expressing cells inhibits deglycosylation of Class I MHC HC molecules.

123 Deglycosylation of CneF-Cap67 did not diminish its DTH a

124 ilar components are produced by acid-induced deglycosylation of DLIF isolated from bovine adrenal cor

125 No increase in current was observed after deglycosylation of extracellular domains of ENaC.

126 lation of 1 indicates that the half-life for deglycosylation of Fapy-dA at 37 degrees C is approximat

127 Deglycosylation of Fapy-dA in the monomer follows first-

128 The rate constants for deglycosylation of Fapy-dA in the monomeric and oligonuc

129 Analysis of the rate constant for deglycosylation of Fapy-dG in an oligonucleotide, reveal

130 ed by autoclave treatment, which also caused deglycosylation of flavonol glycosides.

131 transferase I gene inactivation nor PNGase F deglycosylation of fully processed GC-B reduced GC activ

132 We propose that this deglycosylation of glycoproteins containing high-mannose

133 imity to the proteasome and is available for deglycosylation of glycoproteins destined for degradatio

134 Deglycosylation of glycoproteins using alpha(1-3,4)-fuco

135 onsistent with a role for each in catalyzing deglycosylation of glycoproteins.

136 Moreover, deglycosylation of HepG2 membrane preparations did not a

137 ond pneumococcal neuraminidase, NanB, in the deglycosylation of host glycoconjugates and have demonst

138 Furthermore, deglycosylation of IgG1 resulted in a 40-fold loss in Fc

139 Deglycosylation of immunoprecipitates obtained using the

140 The balance of glycosylation and deglycosylation of ion channels can markedly influence t

141 oteins that utilizes the enzyme PNGase A for deglycosylation of labeled peptic N-linked glycopeptides

142 Deglycosylation of Lamp-1 and Lamp-2 resulted in their r

143 specifically of BaEV and HERV-W, and that N-deglycosylation of mASCT1 activates it as a receptor for

144 y RD114 and type D retroviruses is caused by deglycosylation of mASCT1, which unmasks previously buri

145 Partial deglycosylation of membrane associated FR from either ce

146 Based on this finding, we propose that after deglycosylation of misfolded glycoproteins by PNGase, th

147 Deglycosylation of mouse, human, and bovine LLPLs yielde

148 A. marginale to tick cells because chemical deglycosylation of MSP1a significantly reduced its adhes

149 We show here that deglycosylation of MUC1 results in >75% reduction in CES

150 es mannose trimming, polyubiquitination, and deglycosylation of mutant channels.

151 m neuroligin-1 enhance its activity, whereas deglycosylation of neurexin-1beta did not alter its asso

152 ies in ANP binding was examined by enzymatic deglycosylation of NPR-ECD followed by binding assay.

153 Finally, deglycosylation of p98 did not alter its migration, sugg

154 Deglycosylation of PHF tangles by endoglycosidase F/N-gl

155 nked oligosaccharides on the proteoglycan, N-deglycosylation of phosphacan had no effect on its bindi

156 Prior deglycosylation of pig AE2 unmasked novel, ionic strengt

157 he precursor activity was found to be due to deglycosylation of plasma Gc protein by alpha-N-acetylga

158 Chemical deglycosylation of protein fractions from the 81-176 wil

159 Enzymatic deglycosylation of proteins in the TM media converted th

160 We conclude that deglycosylation of proteins within the first few hours o

161 Following deglycosylation of purified ASGP-R, we detected the H2b

162 analyzed by native gel electrophoresis, and deglycosylation of rat liver lysate had no effect on eit

163 Complete deglycosylation of REJ followed by Western blotting yiel

164 emperatures (130-165 degrees C) promoted the deglycosylation of rutin to produce isoquercetin and sub

165 While interactions strengthen with deglycosylation of SIRPalpha1, low copy numbers explain

166 Deglycosylation of solAPPcyt showed that it contained bo

167 Here, we describe the partial deglycosylation of soluble, cleaved recombinant Env trim

168 Deglycosylation of Sp1 protein also reduced Sp1 binding

169 o complex N-glycans, and following enzymatic deglycosylation of surface N-glycans.

170 Deglycosylation of SZ21 abrogates Fc-effector functions

171 rporation of A opposite OG by catalyzing the deglycosylation of the aberrant adenine.

172 Deglycosylation of the C(H)2 domains abrogated sFc gamma

173 By using deletion constructs and enzymatic deglycosylation of the C-terminal PSGL-1 fragments, the

174 Deglycosylation of the flagellin proteins with trifluoro

175 Deglycosylation of the full-length homotrimer with pepti

176 ient growth is dependent upon the sequential deglycosylation of the glycoconjugate substrate by pneum

177 The method encompasses the enzymatic deglycosylation of the glycoprotein, the permethylation

178 for maturation of the propeptide, enzymatic deglycosylation of the mature wild-type GGT does not sub

179 Enzymatic deglycosylation of the membrane preparations converted b

180 oxo-G:A and G:A mismatches and catalyzes the deglycosylation of the mismatched 2'-deoxyadenosine.

181 stingly, no evidence was found for gas-phase deglycosylation of the N-linked sugar in ribonuclease B,

182 rotein coupling seen with complete enzymatic deglycosylation of the native receptor previously report

183 The enzymatic deglycosylation of the plant flavonoid rutin (quercetin-

184 that the V-shaped conformation is induced by deglycosylation of the protein, and that physiologic gly

185 The binding was inhibited by mannose and by deglycosylation of the proteins prior to the overlay ass

186 Deglycosylation of the purified hEGP completely disrupte

187 Deglycosylation of the receptor with peptide N:glycosida

188 Deglycosylation of the recombinant aggrecan fragment red

189 However, deglycosylation of the recombinant and fungal phytase yi

190 Deglycosylation of the recombinant proteoglycans and cor

191 Gentle partial deglycosylation of the SIgA secretory component enhanced

192 using a bradykinin analog as substrate, and deglycosylation of the wild-type protein resulted in los

193 Following deglycosylation of these biosynthetic precursors, the re

194 the less polar components, suggests in vivo deglycosylation of these factors.

195 eptide product expected from PNGase-mediated deglycosylation of this glycopeptide, namely, R-Asp-X-Th

196 cyclic AMP (cAMP) results in nearly complete deglycosylation of this protein.

197 The possibility of extracellular deglycosylation of type IV collagen was investigated, bu

198 In vitro dephosphorylation, but not deglycosylation, of AD P-tau inhibits its self-associati

199 In this work, the effect of deglycosylation on the electrochemical properties of CDH

200 Specific enzymatic deglycosylation on the intact protein identified the two

201 er molecular weight species as did enzymatic deglycosylation or blockade of glycosylation, and all th

202 Enzymatic deglycosylation or mutation of the N-linked glycosylatio

203 Deglycosylation plus dephosphorylation, but not deglycos

204 ne residues by iodoacetamide and enzymatic O-deglycosylation prior to DTT reaction.

205 crystallizable region followed by overnight deglycosylation prior to LC-HRMS analysis.

206 ing has been integrated with a fast PNGase F deglycosylation procedure that achieves complete deglyco

207 We have developed a deglycosylation procedure that allows the precise identi

208 protein secondary structure results from the deglycosylation procedure.

209 Deglycosylation proceeds via a product-assisted mechanis

210 and free in the cytosol, which suggests the deglycosylation process can proceed at either site depen

211 nium ion intermediate is responsible for the deglycosylation process if the integrity of the pyrimidi

212 2'-deoxyribose isomerization and subsequent deglycosylation processes in two pyrimidine lesions: 5,6

213 yric type A receptor (GABAAR) subunits using deglycosylation protein shift assays.

214 available HPLC-chip to perform glycoprotein deglycosylation, protein removal, glycan capture, glycan

215 The deglycosylation rate was first examined by SDS-PAGE at t

216 igosaccharides from large-scale glycoprotein deglycosylation reactions is the time-consuming chromato

217 bject to proteolysis, Asn glycosylation, and deglycosylation reactions.

218 ffect on activity in solution, but enzymatic deglycosylation reduced Hyal1 activity in a time-depende

219 l MP-mediated protection, because chemical O deglycosylation reduced the protective efficacy of MP im

220 nsistent with the demonstrated glycosylation/deglycosylation requirement, the cytosolic deglycosylati

221 Enzymatic N-deglycosylation resulted in an Epo-Epo species that migr

222 ation site at asparagine 297 of the Fc, with deglycosylation resulting in a complete loss of hFcgamma

223 mpared with traditional protocols (overnight deglycosylation, sample cleanup by graphitized carbon or

224 Chemical and enzymatic deglycosylation show that the differences revealed by th

225 Enzymatic deglycosylation, site-directed mutagenesis, and lectin p

226 Further purification, deglycosylation, specific chemical and enzymatic cleavag

227 es an up-front complete or partial enzymatic deglycosylation step before trypsin digestion to charact

228 A deglycosylation step using Peptide-N-Glycosidase F (PNGa

229 pellet with 60% aqueous methanol after each deglycosylation step.

230 ia-based beta-elimination and hydrazinolysis deglycosylation strategies.

231 Immunocytochemistry, ELISA, and deglycosylation studies indicated that accumulation of m

232 Deglycosylation studies showed that MT4-MMP is modified

233 Deglycosylation studies showed that the recombinant enzy

234 ests that DLIF is 8-fold more susceptible to deglycosylation than is digoxin.

235 of discoidin domain receptor 2, or collagen deglycosylation that prevents discoidin domain receptor

236 A receptor cDNA; following receptor membrane deglycosylation, the antibody detected an additional 40

237 downstream analysis requires relatively long deglycosylation times (from several hours to overnight)

238 an analysis of biopharmaceuticals with rapid deglycosylation times.

239 Deglycosylation treatment attenuated high molecular weig

240 Importantly, deglycosylation treatment with neuraminidase inhibits na

241 Each sLRP was purified to homogeneity after deglycosylation using a combination of anion-exchange an

242 Controlled deglycosylation using peptide : N-glycosidase F and endo

243 Deglycosylation using peptide-N-glycosidase F and site-d

244 The elution pattern and sequence of DLIF-deglycosylation was identical to that of digoxin suggest

245 On-target deglycosylation was performed to confirm the detection o

246 ), time (tau(50)) in which 50% of isoflavone deglycosylation was reached, and time (tau(complete)) re

247 Changes to IgG1 conformation as a result of deglycosylation were determined by comparing exchange in

248 te)) required to achieve complete isoflavone deglycosylation, were 0.16+/-0.02 min(-1), 4.54+/-0.32 m

249 hese aggrecan preparations following enzymic deglycosylation, were compared.

250 or rTF(1-263)-FVIIa remained unchanged after deglycosylation, whereas the k(cat) decreased slightly.

251 d, in k(cat) was observed for pTF.FVIIa upon deglycosylation, whereas the K(m) was minimally altered.

252 terminal glycopeptides and was unaffected by deglycosylation, whereas the smaller rabbit AE2 C-termin

253 fector function by selective IgG heavy-chain deglycosylation with bacteria-derived endoglycosidase S

254 d of identical size to CNS-type NMDAR1 after deglycosylation with endoglycosidase F/peptide-N-glycosi

255 6 activity was not specifically inhibited by deglycosylation with peptide N-glycosidase F.

256 S-7 cells transiently transfected with GPVI, deglycosylation with peptide-N-glycosidase F (PNGase F;

257 A and beta 4 subunits in Xenopus oocytes and deglycosylation with peptide-N-glycosidase F.

258 Blocking required IgG glycosylation because deglycosylation with peptide:N-glycanase eliminated bloc

259 Deglycosylation with protein-N-glycosidase F (PNGase F)

260 ng region between the domains and subsequent deglycosylation yielded the individual EGF-like domains.