ライフサイエンスコーパス: degradosome

1 unlikely to be extensively folded within the degradosome.

2 lytic "machine" that has been termed the RNA degradosome.

3 3'-exoribonuclease and component of the RNA degradosome.

4 nd for the regeneration of ATP by PPK in the degradosome.

5 d RhlB are two other major components of the degradosome.

6 r the organization and mechanisms of the RNA degradosome.

7 exoribonuclease, DSS1, to function as an RNA degradosome.

8 ate the destruction of such RNAs by the mRNA degradosome.

9 for the complex to serve as an effective RNA degradosome.

10 rapid decay possibly facilitated by the mRNA degradosome.

11 ode of action, and its assembly into the RNA degradosome.

12 rganizes the other protein components of the degradosome.

13 ll-sized mRNAs at (UUC)n runs by the E. coli degradosome.

14 of a large RNA-catalysis machine termed the degradosome.

15 er proteins to form a complex termed the RNA degradosome.

16 arget specific classes of mRNAs for decay by degradosomes.

17 - a major component of E. coli RNase E-based degradosomes.

18 erties of recombinant and cell-extracted RNA degradosomes.

19 s into the structure and dynamics of the RNA degradosome, a fascinating and complex macromolecular as

20 the carboxy-terminal half organizes the RNA degradosome, a multi-enzyme complex that degrades mRNA c

21 ) is a component of the Escherichia coli RNA degradosome, a multiprotein complex that also includes R

22 that the functional requirements of SUV3 for degradosome activity and maintenance of mtDNA stability

23 le in mtDNA maintenance independently of its degradosome activity.

24 RhlB, the RNA helicase component of the degradosome, also contributes to ribosome binding, and t

25 ized RNase RnpA interacts with the S. aureus degradosome and that this interaction is conserved in ot

26 ffecting both the protein composition of the degradosome and the endonucleolytic activity of RNase E.

27 d to poly(A) tails may link the functions of degradosomes and ribosomes.

28 Thus, PPK in the degradosome appears to maintain an appropriate microenvi

29 We conclude that the constituents of the RNA degradosome are components of the E. coli cytoskeleton,

30 observations for the organization of the RNA degradosome are discussed.

31 he principal components of the C. crescentus degradosome are the endoribonuclease RNase E, the exorib

32 the isolation and characterization of an RNA degradosome assembly from the alpha-proteobacterium Caul

33 A similar RNA degradosome assembly has been described in the alpha-pro

34 Disruption of degradosome assembly in mutants lacking the polynucleoti

35 many bacterial species, the multi-enzyme RNA degradosome assembly makes key contributions to RNA meta

36 e RhlB is a component of the multienzyme RNA degradosome assembly, and its interaction with the core

37 cterized as the central component of the RNA degradosome assembly.

38 uction, depending on context, within the RNA degradosome assembly.

39 ease RNase E as part of the multi-enzyme RNA degradosome assembly.

40 portant role in the maintenance of the PAP I-degradosome association during stationary phase.

41 tial and stationary phases and find that the degradosome can contain up to 10 different proteins unde

42 and RNA helicase B (RhlB) components of the degradosome can each independently form coiled structure

43 We present evidence that the degradosome can form a stable complex with the 70S ribos

44 The key component of the degradosome complex is the endoribonuclease RNase E, a m

45 s a scaffold for formation of a multiprotein degradosome complex modulates the inhibition of RNase E

46 gulation of manX did not require the RNase E degradosome complex, suggesting that the primary mechani

47 r their 5'-end and is a component of the RNA degradosome complex, which also contains the 3'-exonucle

48 which is thought to exist with RNase Y in a degradosome complex.

49 nd integral component of the multienzyme RNA degradosome complex.

50 Here we report that an additional degradosome component is the essential exoribonuclease R

51 udies showed that RNaseE and the other known degradosome components (RNA helicase B, polynucleotide p

52 ted in vitro the binary interactions between degradosome components and have characterized interactio

53 e E may thus act as a flexible tether of the degradosome components.

54 ng proteins that modulate the remodelling of degradosome composition in bacteria and argue that such

55 distinct, and inhibitor-specific changes in degradosome composition--and that these are in turn asso

56 It was recently shown that the major degradosome constituents are organized in a coiled cytos

57 lution in bacteria carrying mutations in the degradosome constituents RNase E, polynucleotide phospho

58 ins, affected differentially by mutations in degradosome constituents, or not detectably altered by d

59 kely to comprise a basic core element of the degradosome cytoskeletal structures.

60 evidence that PAP I interacts with the mRNA degradosome during both exponential and stationary phase

61 h the RNA helicase RhlB independently of RNA degradosome formation in Escherichia coli.

62 nsuspected effects of RNase E deficiency and degradosome formation on nutrient utilization by E. coli

63 lyses to an E. coli strain with impaired RNA degradosome formation, we investigated the biological co

64 eveloped a procedure to reconstitute the RNA degradosome from recombinant components using modular co

65 n mRNAs is abolished by dissociating the RNA degradosome from the membrane.

66 s the implications for the regulation of RNA degradosome function in vivo.

67 We show that the degradosome has an ATP-dependent activity that aids the

68 The recruitment of enolase by the RNA degradosome has been implicated in the turnover of certa

69 processing of structural precursors, the RNA degradosome has differential activities on a spectrum of

70 hydratase), which is a component of the RNA degradosome, has been determined at 2.5 A.

71 ll-length RNase E, which allows formation of degradosomes, have nutritional requirements different fr

72 was found to inhibit RNA degradation by the degradosome in vitro.

73 ase RNase E and its associated proteins (the degradosome) in negative regulation by these sRNAs.

74 mily and for the assembly of the E. coli RNA degradosome, in which RNase E is the central component.

75 When the enolase-RNase E/degradosome interaction is disrupted, the anaerobically

76 endent assembly of the RhlB component of the degradosome into polymeric filamentous structures in vit

77 The RNA degradosome is a multi-enzyme assembly that contributes

78 The Escherichia coli RNA degradosome is a multi-enzyme complex that contains the

79 The Escherichia coli RNA degradosome is a multienzyme assembly that functions in

80 The Escherichia coli degradosome is a multienzyme complex with four major pro

81 The RNA degradosome is a multiprotein macromolecular complex tha

82 The Escherichia coli RNA degradosome is a protein complex that plays a critical r

83 The putative role of enolase in the RNA degradosome is discussed; although it was not possible t

84 s the fact that the presence of a functional degradosome is essential for RNase E to detect increased

85 However, the role of enolase in the RNase E/degradosome is not understood.

86 Here we demonstrate that the RNase E-based degradosome is required for poly(A) polymerase I (PAP I)

87 that of the core complex of the E. coli RNA degradosome lacking RNase E but very different from that

88 Recently, a degradosome-like complex identified in Bacillus subtilis

89 Recent studies have provided evidence for a degradosome-like complex inB. subtilisthat is built arou

90 sis identified the Staphylococcus aureus RNA degradosome-like complex to include RNase J1, RNase J2,

91 matic activity, and suggest the existence of degradosome-like complexes in Gram-positive bacteria.

92 that enolase is recruited into putative RNA degradosome machinery in these bacilli, where it plays c

93 ts indicate that RNase E associated with the degradosome may contribute to the removal of poly(A) tai

94 n the coding sequences in both wild type and degradosome mutant strains.

95 y located within the coding sequences in the degradosome mutants compared to the wild type control wh

96 yhB is dependent on RNase E and is slowed in degradosome mutants.

97 e constituents, or not detectably altered by degradosome mutations.

98 component of the Pseudomonas aeruginosa RNA degradosome, occluding them from substrates and resultin

99 The mitochondrial RNA degradosome of budding yeast (mtEXO) has been recently s

100 The RNA degradosome of Escherichia coli is a multiprotein comple

101 here that the assembly of the RNase E-based degradosome of Escherichia coli is not required for norm

102 RNaseE is the main component of the RNA degradosome of Escherichia coli, which plays an essentia

103 inct from those found in proteobacteria, the degradosomes of which are assembled around the unstructu

104 n of PNPase because association with the RNA degradosome or with RNA helicase B (RhlB) is not require

105 icing through RNA degradation as part of the degradosome, or has a direct role in splicing as a chape

106 donuclease RNase E and components of the RNA degradosome, particularly enolase, a glycolytic enzyme w

107 The degradosome plays a key role in RNA processing and degra

108 , we identify an additional component of the degradosome, polyphosphate kinase (PPK), which catalyses

109 The results indicate that the RNA degradosome proteins are components of the bacterial cyt

110 The Escherichia coli RNA degradosome proteins are organized into a helical cytosk

111 degradosomes, whereas others are acted on by degradosome proteins functioning independently of the co

112 , the cytoskeletal organization of the other degradosome proteins required the presence of the RNaseE

113 ndance were affected congruently by multiple degradosome proteins, affected differentially by mutatio

114 f-recognition and interaction with the other degradosome proteins.

115 oiled structures in the absence of the other degradosome proteins.

116 composition in bacteria and argue that such degradosome remodelling is a mechanism for the different

117 3 may serve in part as a component of an RNA degradosome, resembling its yeast ancestor.

118 Defects in the degradosome result in accumulation of aberrant transcrip

119 ctionally and physically, respectively, with degradosome ribonucleases.

120 f ribosomal proteins and the scaffold of the degradosome, RNase E.

121 To investigate the degradosome's proposed role as an RNA decay machine, we

122 When both the degradosome scaffold region and the ARRBS are missing, m

123 h segments resemble the C-terminally located degradosome scaffold region of E. coli RNase E.

124 he rates of capture of the limited number of degradosomes, so that they become recruited to sites of

125 bic conditions, enolase bound to the RNase E/degradosome stabilizes the small RNA (sRNA) DicF, i.e.,

126 tant role in the membrane association of the degradosome system.

127 nent ribonucleolytic complexes known as "RNA degradosomes." These complexes consist of four major com

128 to a multi-enzyme assembly, known as the RNA degradosome, through an interaction with the scaffolding

129 of the ribonucleolytic activities within the degradosome to 3' attack since previous studies had only

130 t that polyadenylation requires a functional degradosome to maintain an equilibrium between free PNPa

131 Suv3p acts indirectly as a component of the degradosome to promote aI5beta splicing.

132 mechanism by which Ecoli uses enolase-bound degradosomes to switch from rod-shaped to filamentous fo

133 processing and degradative activities of the degradosome, to regulate their access to important cellu

134 port that presence of enolase in the RNase E/degradosome under anaerobic conditions regulates cell mo

135 ctive PNPase protein that still forms intact degradosomes, we show that a catalytically active PNPase

136 s in vivo depends on the action of assembled degradosomes, whereas others are acted on by degradosome

137 is a critical component of the mitochondrial degradosome, which also includes a 3' --> 5' exonuclease

138 tic enzyme enolase is a component of the RNA degradosome, which is an RNase E mediated assembly invol

139 Incubation of the degradosome with affinity-purified antibody against RhlB