ライフサイエンスコーパス: denaturation

1 ed by analyzing nanomechanically induced DNA denaturation.

2 t results concerning the effects of chemical denaturation.

3 ed for extensive oxidation-dependent protein denaturation.

4 common unfolding event that reflects global denaturation.

5 lpha-Gal IgE epitopes were resistant to heat denaturation.

6 age or stress-induced protein misfolding and denaturation.

7 ater and in influencing chain expansion upon denaturation.

8 et structures, all of which indicate protein denaturation.

9 inhibition activity, which was removed upon denaturation.

10 s of SEE in preventing enzyme aggregation or denaturation.

11 ility of both serum albumins against thermal denaturation.

12 s of samples down to 1.9 mN/m due to protein denaturation.

13 proteins, resulting from stress such as heat denaturation.

14 at is resistant to both pH- and heat-induced denaturation.

15 ting of amylose-lipid complexes plus protein denaturation.

16 arbohydrate-protein interaction, and protein denaturation.

17 ltodextrin using the effect of pH on protein denaturation.

18 or activity and is very resistant to thermal denaturation.

19 teractions relevant in urea-assisted protein denaturation.

20 raditionally explored in chemical or thermal denaturation.

21 tion of the protein with respect to chemical denaturation.

22 a co-solute, recorded via a delayed thermal denaturation.

23 ned by resistance to thermal and/or chemical denaturation.

24 ble-helical structure of DNA against thermal denaturation.

25 tects myosin against heat stress and thermal denaturation.

26 they first undergo proteolytic processing or denaturation.

27 uate conformational changes as a function of denaturation.

28 r or sliding clamp) obtained during chemical denaturation.

29 can lead, possibly irreversibly, to protein denaturation.

30 c activities after heat/guanidinium chloride denaturation.

31 e and protein solubility, indicating protein denaturation.

32 hat the fluorescence of GFP is quenched upon denaturation.

33 how markedly enhanced sensitivity to thermal denaturation.

34 cording to Privalov's interpretation of cold denaturation.

35 ation treatment for 10min indicating protein denaturation.

36 from that of Post-F-XC, as shown by chemical denaturation.

37 l conditions (room temperature) without heat denaturation.

38 lower protein yield as well as less protein denaturation.

39 erved that adsorbed proteins undergo greater denaturation.

40 h these epitopes are revealed with guanidine denaturation.

41 n a sequence-specific manner without causing denaturation.

42 enature proteins, i.e., thermal and chemical denaturation.

43 east frataxin, a protein that undergoes cold denaturation above zero degrees, because the unfolded fo

44 ability in bulk solution, as well as thermal denaturation, aggregation state, and biological activity

45 Starch swelling and denaturation/aggregation of proteins occurring at divers

46 eir different stability against urea-induced denaturation allowed us to discriminate them, even when

47 e. duration or temperature) induced complete denaturation, an increase of surface hydrophobicity and

48 ular dichroism spectra in far UV and thermal denaturation analyses demonstrate that axial ligand chan

49 y, and we have mapped genomic features using denaturation analysis.

50 terminal domain of the protein and cause its denaturation and aberrant cytoplasmic translocation.

51 The patterns of denaturation and aggregation varied with pH.

52 noclonal antibodies (mAb) to prevent protein denaturation and aggregation.

53 e heat stability, and decreased whey protein denaturation and aggregation.

54 cedure included sodium dodecyl sulfate (SDS) denaturation and chemical reduction of serum proteins to

55 zwitterionic gel coating protected BChE from denaturation and degradation under harsh conditions.

56 ature of proteins with strong resistances to denaturation and degradation.

57 on allows for natural expansion upon protein denaturation and dissociation.

58 were used to evaluate the effects of protein denaturation and disulfide bonds on zein's ability to fo

59 gested by the Lumry-Eyring model for protein denaturation and enzyme inactivation.

60 t activity; however, processing leads to its denaturation and hence reduced hydrolysis.

61 reduces disulfide bonds and can drive mucin denaturation and microbial access to the mucus layer.

62 overcome the instability issues from thermal denaturation and nonspecific electrostatic binding while

63 formation using UV- and CD-monitored thermal denaturation and on nucleoside conformation using (1)H N

64 CP addition prevented freeze-induced denaturation and oxidation in all extracted proteins, as

65 stals growth in NAM to reduce protein freeze-denaturation and oxidation similarly to the commercial c

66 ivo complete protein digestion requires acid denaturation and pepsin, trypsin, and/or chymotrypsin cl

67 xtremely stable against thermal and chemical denaturation and proteolytic degradation.

68 Denaturation and refolding behavior of the allergen conf

69 c acid amplification methods, controlled DNA denaturation and renaturation is particularly essential

70 lectrochemical control of five cycles of DNA denaturation and renaturation, under otherwise constant

71 to drive cyclic electrochemically controlled denaturation and renaturation.

72 Denaturation and subsequent aggregation of beta-lactoglo

73 d in SDS-stabilised emulsions due to protein denaturation and subsequent exposure of previously burie

74 ways when pH is decreased from 7 to 5: Urea denaturation and temperature stability assays show that

75 Equilibrium denaturation and temperature-jump relaxation experiments

76 extremely resistant to chemical and thermal denaturation and that resist proteolysis by common prote

77 exceptionally stable to thermal and chemical denaturation, and 12 experimentally determined X-ray and

78 nates the need for enzymatic reactions, heat denaturation, and complicated instruments during the det

79 ght of morphological alterations and protein denaturation, and it is concluded that LF NMR relaxometr

80 cts such as non-specific adsorption, protein denaturation, and steric hindrance.

81 to dye or protease, more stable to chemical denaturation, and widely stabilized against NMR-detected

82 changes in Trp fluorescence induced by urea denaturation are used to construct denaturation curves f

83 ultant flours seems to be related to protein denaturation as shown by decrease in nitrogen solubility

84 ities of these membrane proteins to chemical denaturation, as judged by their m values, was consisten

85 degrade at room temperature over time due to denaturation, as proteins unfold with loss of secondary

86 clic polyamides were measured by DNA thermal denaturation assays and compared to the corresponding ha

87 Herein, we use electrochemically driven denaturation assays monitored by surface-enhanced Raman

88 exhibit their highest stability against heat denaturation at pH 3.75.

89 Contrary, denaturation at pH 6.8 and 8.0 led to formation of non-n

90 ential scanning calorimetry indicated myosin denaturation at the surface of the sample treated with t

91 DeltaC1/2%, we compared relative changes of denaturation between peptides.

92 ueous solution, pushing the solution thermal denaturation beyond the boiling point of water.

93 Results from thermal denaturation, binding energy, and recognition experiment

94 structures, MANT-GTPgammaS binding, thermal denaturation, biochemical assays and sequence homology a

95 p and retain carbon monoxide, which prevents denaturation both in vitro and in vivo and explains the

96 a novel conformational regime for DNA, where denaturation bubbles form at the tips of plectonemes, an

97 e double helix and the presence of transient denaturation bubbles.

98 , non-modified Cyt c was more susceptible to denaturation by a water-organic solvent interface than i

99 nprotein matrix components, and the thorough denaturation by both surfactant and precipitation enable

100 the change in heat capacity and enthalpy of denaturation by differential scanning calorimetry, and t

101 can resist heat, pressure, proteolysis, and denaturation by reagents such as urea or sodium dodecyl

102 abilization of the Skp trimer and similar to denaturation by the nonnatural detergent lauryldimethyla

103 nomic fragmentation by ultrasounds and dsDNA denaturation by thermal treatment were also investigated

104 The large effect of PEG 20 on cold denaturation can be explained by a change in water activ

105 enomic loci fluorescently without global DNA denaturation (Cas9-mediated fluorescence in situ hybridi

106 ge 20-50%, a sharp increase in the degree of denaturation, compared to the native protein solution, w

107 d by urea denaturation are used to construct denaturation curves from which unfolding free energies c

108 While denaturation curves obtained after exposure of PrP(Sc) t

109 The thermal denaturation data were analyzed according to the Lyubare

110 ecule force spectroscopy or existing thermal denaturation data.

111 present study demonstrated that serum sample denaturation decreases the test specificity due to relea

112 e-heated WPI had a significant impact on the denaturation degree and on the surface hydrophobicity, r

113 trophotometric data for determining the heat denaturation degree of bovine serum albumin (BSA) was as

114 well as increased stability against chemical denaturation [DeltaCm (GndHCl) = 0.53 M], without alteri

115 colour from Chromametry, enthalpy change of denaturation (DeltaHcal) from DSC and molar ellipticitie

116 riment also supports the picture of chemical denaturation described in our simulations, driven by wea

117 iciency, which is not alleviated by altering denaturation/digestion stringency and indicates why prot

118 Denaturation disrupts the conformation of albumin so tha

119 spatial extent and the degree of the tissue denaturation due to the cauterization.

120 ion times indicated a lower degree of muscle denaturation during ageing in the chitosan dry-ageing be

121 lureas such as N-ethylurea provide increased denaturation during cIEF.

122 The proteins of the IPPC underwent denaturation during extraction and some of the starch su

123 00 W/cm(2), 2078 J/mL) significantly reduced denaturation enthalpies, whereas no change in secondary

124 e then characterized with respect to thermal denaturation, enzymatic stability, and fluorescence prop

125 enough to detect changes in the protein heat denaturation even at short temperature-time intervals.

126 Therefore, isothermal chemical denaturation expands the available toolkit of biophysica

127 The reason for this shift is that chemical denaturation experiments are labour-intensive, sample-co

128 in the presence of dextran 20, and chemical denaturation experiments at different fixed temperatures

129 Use of dynamic light scattering and thermal denaturation experiments delineates the compaction of th

130 Differential scanning fluorometry and urea denaturation experiments demonstrate the impact of parti

131 ans of surface plasmon resonance and thermal denaturation experiments finding that the position of th

132 This was confirmed with pH and urea denaturation experiments in conjunction with electron mi

133 ed in oligonucleotide sequences, and thermal denaturation experiments indicate that it is destabilizi

134 Consistently, urea denaturation experiments indicate the stability of the W

135 Thermal denaturation experiments of oligonucleotide sequences co

136 This interpretation is supported by urea denaturation experiments performed on both PrP variants

137 Thermal denaturation experiments revealed that hm(5)rC increases

138 D-ring photoflip in canonical phytochromes, denaturation experiments showed conclusively that the sa

139 Their DNA affinity was evaluated by thermal denaturation experiments using salmon sperm DNA.

140 Invader probes are characterized by thermal denaturation experiments, analysis of thermodynamic para

141 tion mechanism of 1-3 has been determined by denaturation experiments, which reveal that the formatio

142 rative transition observed by classical urea denaturation experiments.

143 which was evaluated by means of DNA thermal denaturation experiments.

144 opy in combination with thermal and chemical denaturation experiments.

145 The first strategy, using off-chip heat denaturation followed by sequential on-chip incubation o

146 se-2 is a dimeric enzyme that undergoes cold denaturation following a highly cooperative N2 2I mechan

147 Isothermal chemical denaturation has been shown to be a valuable biophysical

148 Thermal denaturation has irreversible destabilizing effects on b

149 occuring AR domain of human IkappaBalpha to denaturation; however, only the YLTA mutations stabilize

150 ew of the non-locality of sequence-dependent denaturation in highly bent and supercoiled DNA loops, e

151 s range, which correlated with the extent of denaturation in the adsorbed state.

152 e epitope was sensitive to reduction and SDS denaturation in the isolated ricin domain and the larger

153 rt, by ConA's instability due to its thermal denaturation in the physiological environment (37 degree

154 of each lysine as a function of acetonitrile denaturation in the present study support that PARP-C ex

155 amic stability of each domain by equilibrium denaturation in urea.

156 Protein denaturation increased the reaction rate and reduced the

157 proteins decreased in the treated-milk, and denaturation increased with the treatment intensity.

158 both prion types to guanidine hydrochloride denaturation indicated this occurs because NAPA preclude

159 biological fluids can be compromised through denaturation induced by physiological salt concentration

160 efficiency, which is shown to correspond to denaturation-induced looped conformations that remain st

161 alysis reveal that the first step of thermal denaturation involves dissociation of subunits III, VIa,

162 This low degree of heat denaturation is probably caused by the aggregation of th

163 of several individual domains due to thermal denaturation is reduced upon their fusion into multi-dom

164 Although chemical-induced denaturation is the gold-standard method, it has been re

165 f newly synthesized protein or after protein denaturation, is a substrate for binding by many cellula

166 We obtain protein denaturation kinetics as a function of temperature, and

167 ovel method to measure protein stability and denaturation kinetics, on unprecedented timescales, thro

168 for heat stability, rheological properties, denaturation level of beta-lactoglobulin (beta-LG) and a

169 tate has a higher susceptibility to pressure denaturation (lower p1/2 and larger DeltaVu); thus, urea

170 onstrating DNA nanochannel-based stretching, denaturation mapping, and partitioning/trapping of singl

171 Thermal denaturation measurements and structural comparisons of

172 cy therefore challenges our understanding of denaturation mechanism and more generally the accuracy o

173 create a high-throughput label-free chemical denaturation method that is capable of generating replic

174 itration calorimetry and equilibrium thermal denaturation methods and were found to be significantly

175 -controlling effect of CCH on protein freeze-denaturation, NAM samples were subjected to 7 freeze-tha

176 al antibodies and to detection of partial pH denaturation of a monoclonal antibody illustrates the pr

177 as found to exhibit a maximum degree of heat denaturation of about 17% compared to the complete unfol

178 rmally activated processes contribute to the denaturation of adsorbed proteins.

179 present fully atomistic simulations of cold denaturation of an alpha-helical protein, the widely stu

180 tical model was developed to explain how the denaturation of an individual, adsorbed protein molecule

181 The reversible thermal denaturation of apo alpha-lactalbumin (alpha-LA) and lys

182 HHT at pH 6.6 resulted in denaturation of approximately 67% of total whey protein,

183 cooking with wood ashes and Ca(OH)2 produced denaturation of bean proteins, decreasing the second tra

184 Hence, in this study total denaturation of beta-lactoglobulin was performed at defi

185 hydryl-disulfide interchange reaction during denaturation of beta-LG and alpha-LA.

186 tor the temperature-dependent adsorption and denaturation of bovine serum albumin (BSA) protein onto

187 escribe the complex mechanism of the thermal denaturation of CcO, the obtained results were utilized

188 all fraction of polypeptides could lower the denaturation of crayfish myosin heavy chain when compare

189 Thermal denaturation of dodecyl maltoside solubilized CcO procee

190 ibrium reached between the hybridization and denaturation of double-stranded DNA (ds-DNA) is opened u

191 N-ethylurea and 8M urea provided sufficient denaturation of ErA, resulting in a main peak with a pI

192 on Gr through the FAD-domain and/or partial denaturation of HMP.

193 -MS methodology was used to monitor the mild denaturation of horse heart myoglobin by acetonitrile, a

194 lded (denatured) state, we study the thermal denaturation of hydrated lysozyme that occurs when the t

195 Notably, the denaturation of MBD1 disassembled the DMA and accordingl

196 Ionic-liquid-induced denaturation of one of the enzymes is indicated over the

197 sed in the process of crystallization causes denaturation of pathological tissue proteins (metastasis

198 ential conformational changes due to partial denaturation of PPIs.

199 inization of starch in the flour and partial denaturation of proteins.

200 However, in vivo degradation or denaturation of surface constituents often limits the lo

201 nd omega-3 oil did not interfere with normal denaturation of surimi proteins.

202 e complete starch gelatinization and protein denaturation of the bean powders and thus changed their

203 Denaturation of the chimeric collagen increased its affi

204 measurement cycles is attributed to partial denaturation of the enzyme.

205 haw damage by preventing the aggregation and denaturation of the enzyme.

206 ated brine, increasing the risk of microbial denaturation of the fillets during storage.

207 1 is demonstrated by monitoring the thermal denaturation of the following double and triple helical

208 ading to stronger networks following thermal denaturation of the globular protein.

209 haracterized by the states of ionization and denaturation of the immobilized urease, as such the mode

210 g test without the need for purification and denaturation of the PCR products.

211 Denaturation of the protein causes a sharp drop in the c

212 Monitoring fluorescence during thermal denaturation of the protein in the presence of the dye i

213 and fluorescence spectroscopy confirmed that denaturation of the proteins during the acid precipitati

214 sting real-time RT-PCR assays, which require denaturation of the RVA double-stranded RNA (dsRNA) prio

215 t of the acceptor as an iron-sulfur cluster, denaturation of the SAM-treated protein with acid result

216 Interestingly, there is no concomitant denaturation of the secondary structural elements of Rsn

217 tranded plasmid (ds-Pl) without the need for denaturation of the target plasmid sample using a peptid

218 control at temperatures 60 degrees C caused denaturation of the whey proteins and aggregation of the

219 ottled white wines is attributed to the slow denaturation of unstable proteins, which results in thei

220 heat, pH, ultrasonication and ethanol on the denaturation of whey protein isolate.

221 The enthalpy change (DeltaH of denaturation) of whey proteins decreased in the treated-

222 ion of the therapeutic CD38 antibody or CD38 denaturation on reagent red blood cells mitigates daratu

223 the effect of temperature-dependent protein denaturation on the corresponding adsorption kinetics.

224 in and the influence of pH value and protein denaturation on this reaction have been examined in the

225 idual ribbons is realized either via thermal denaturation or by addition of a DNA separator strand.

226 ion technique without the need of additional denaturation or cross-linking steps for stabilization.

227 , NFs are resistant to nuclease degradation, denaturation or dissociation at extremely low concentrat

228 Food processing causes decomposition, denaturation or polymerization of protein, which may alt

229 an interface is an arrested state along the denaturation pathway.

230 e findings suggest that thermal and chemical denaturation perturb the structure of prion protein aggr

231 Although pressure denaturation perturbs secondary structure less than temp

232 her derive from our observations a schematic denaturation phase diagram together with energy landscap

233 e which, in conjunction with a short thermal denaturation, prevents renaturation of the duplex nuclei

234 igh-resolution structural models of the urea denaturation process of an alpha-helical membrane-associ

235 the unfolding and aggregation phases of the denaturation process, whereas such a discrimination coul

236 ovide valuable insights into details of silk denaturation processes that might be active during spinn

237 gnificant strain-specific differences in the denaturation profile of the protein, treatment with guan

238 ding kinetics and thermodynamics (e.g., melt/denaturation profile) in a single experiment.

239 The individual fit of the pressure denaturation profiles gives rise to first order, GNMR, a

240 using fluorescence polarization and thermal denaturation profiling.

241 bs secondary structure less than temperature denaturation, protein refolding after a fast P-jump is n

242 h rasburicase (known to be very sensitive to denaturation), proving the structural stability of the p

243 nvolve bacterial culture, protein isolation, denaturation, purification and finally protein assembly.

244 ing at this pH value, the supplementary heat denaturation rate was lower compared to that at neutral

245 The highest heat denaturation rates were observed at pH values higher tha

246 likely because surfactant permits extensive denaturation/reduction/alkylation of proteins and inacti

247 was confirmed using limited proteolysis and denaturation/refolding.

248 itive because traditional models of chemical denaturation rely on changes in solvent-accessible surfa

249 -PCR) strategy by exploiting ion-induced DNA denaturation/renaturation cycles.

250 to thermal cycling, 30 cycles of pH-induced denaturation/renaturation were used to amplify protein D

251 , and ultraviolet-visible (UV-vis) monitored denaturation/renaturation.

252 imitations in quantitative protein transfer, denaturation, solubility, or stability.

253 ea that accounts for both the ionization and denaturation states of the urease subject to the environ

254 ause it does not require an antecedent dsRNA denaturation step, this assay reduces the possibility of

255 Thermal denaturation studies and footprinting experiments confir

256 According to the thermal denaturation studies by UV spectroscopy, the effect of t

257 Denaturation studies demonstrate that the stability of C

258 The use of thermal denaturation studies in conjunction with UV spectrophoto

259 erent duplexes that were analyzed by thermal denaturation studies.

260 f fat melting (Tmelting) or xanthine oxidase denaturation (Tdenaturation), whereas thermal treatments

261 ed SCRII was much more thermostable with its denaturation temperature (Tm) increased to 60 degrees C.

262 ed over a temperature range that spanned the denaturation temperature (Tm) of hybrids by applying a s

263 former decreased sigmoidally at the onset-of-denaturation temperature (TOD (FTIR)) of 69.5 degrees C,

264 lly active E. coli DHFR mutant with measured denaturation temperature 7.2 degrees C higher than WT.

265 t was found that PBPI obtained a much higher denaturation temperature of 110.2 degrees C compared to

266 The denaturation temperature of FPI was 105 degrees C.

267 The connective tissue denaturation temperature was lower for the FP group, coi

268 energy of the native state and experimental denaturation temperature was much weaker, indicating the

269 urant, source of enzyme, sample cleanup, and denaturation temperature, among others.

270 alpha1-PI showed high thermal stability with denaturation temperatures of 65.43 and 73.31 degrees C,

271 olo-Lf in aqueous solution displayed thermal denaturation temperatures of 71+/-0.2 and 91+/-0.5 degre

272 The reduced thermal denaturation temperatures of the T61A, S93A, and H266A F

273 cular dichroism spectroscopy to measure half-denaturation temperatures, our results show that protein

274 CPI was more susceptible to thermal denaturation than GCPI as determined by turbidity and su

275 eared meat tended to show less oxidation and denaturation than outdoor-reared meat and was characteri

276 were more sensitive to oxidation and thermal denaturation than those from beta-red fibres.

277 By thermal denaturation, the MOSP(N) and MOSP(C)-like domains of Tp

278 with very high firmness without whey protein denaturation; the firmness was similar to gels from heat

279 Upon refolding after chemical denaturation, this protein produced equimolar amounts of

280 able with the human eye as a function of the denaturation time.

281 We exploit chemical denaturation to determine the unfolding free energy of L

282 esonance energy transfer (FRET) and chemical denaturation to observe and manipulate RNA dynamics.

283 eriments (ultraviolet absorption for thermal denaturation) to analyze the differential stability of d

284 mounts of isobaric label (levels that induce denaturation) to enhance the sensitivity and resolution

285 we observed either two-state or three-state denaturation transitions.

286 tant was designed to allow the study of cold denaturation under near native conditions.

287 odifications, reflected in decreased thermal denaturation values (DeltaTm, ca. 2.5-11.5 degrees C).

288 tudy, the application of isothermal chemical denaturation was applied to an integral membrane protein

289 Xanthine oxidase denaturation was approximately 13% less after PEF treatm

290 ary model based on the mechanisms of protein denaturation was then added to take into account the eff

291 copper protein azurin (Az), from 80 K until denaturation, whereas for apo-Az ETp was temperature dep

292 alternative approach is the use of chemical denaturation, which can be implemented at any temperatur

293 a diverse behavior when subjected to thermal denaturation, which is exceptional as all lasso peptides

294 ction takes place without detectable protein denaturation, which might be revealed by heme loss or an

295 es in the treated samples related to protein denaturation, which was probably responsible for the dec

296 , i.e. 2.5, they exhibited considerable cold denaturation, while after heating at this pH value, the

297 No evidence is observed for denaturation with exposure to any of the organic vapors

298 of duplex formation using ITC and UV thermal denaturation with RNA duplexes containing internal s(2)U

299 proteins is observed in the case of chemical denaturation, with the Cys-less mutant exhibiting lowere

300 ques to study the sequence-dependence of DNA denaturation within DNA minicircles, we have observed th