ライフサイエンスコーパス: denature

1 io-receptors that interact with Cu(2+) to be denatured.

2 tic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions.

3 mor treated with unlabeled alphaGPC3 or heat-denatured (89)Zr-alphaGPC3 demonstrated tumor activity (

4 Denatured AChE was eluted with 1% trifluoroacetic acid.

5 e adapted and used a highly stringent Tandem Denaturing Affinity Purification (TDAP) method, coupled

6 Apple PPO was thermolabile, denaturing after 10min at 70 degrees C.

7 3 activity on full-length VWF arises without denaturing agent as evidenced by FCS and gel-based multi

8 We show that denaturing agents can alter digestion by reducing protea

9 Use of alkylureas as denaturing agents in capillary electrophoresis and cIEF

10 proaches studying the effect of proteases or denaturing agents on the physicochemical properties of P

11 high inherent susceptibility to reducing and denaturing agents, it is very stable upon immobilization

12 t during demineralization or with the use of denaturing agents.

13 from its structured state in the absence of denaturing agents.

14 The trypsin-hydrolyzed denatured albumin exhibited the highest antioxidant acti

15 n, demonstrated by measured KD of native and denatured albumin to gold surafce, 5.8+/-0.2x10(-5) M an

16 albumin is significantly higher than that of denatured albumin, demonstrated by measured KD of native

17 rough the competitive adsorption between the denatured albumins and the native ones, the displacement

18 ning the data from top-down MS of native and denatured aldolase complexes, a total of 56% of the tota

19 2E8, while DDM was additionally added to SDS-denatured alphaLA and betaLG.

20 without its prosegment (PS), is irreversibly denatured and folds to a thermodynamically stable, non-n

21 fied with cow protein isolates that had been denatured and glycated by thermal treatment.

22 GroES assists folding of a broad spectrum of denatured and misfolded proteins.

23 s a sufficiently high barrier separating the denatured and native states that folding appears coopera

24 y populated on parallel pathways between the denatured and native states.

25 SDCPI was the least denatured and VDCPI the most denatured, demonstrating th

26 The AS sodium dodecyl sulfate (SDS) denatures and unfolds globular proteins under most condi

27 the ability to run the samples in different denaturing and nondenaturing buffer allows defining true

28 nformation, binds to a variety of deposited, denatured, and aggregated proteins, in addition to bindi

29 peanut, and pistachio flours using three non-denaturing aqueous (phosphate, borate, and carbonate) bu

30 single and interactive effects of three non-denaturing aqueous (phosphate, borate, and carbonate) bu

31 igomers in vitro, but only the rice isoforms denature at nonpermissive temperatures.

32 purified tAlv-a1-pHCl expressed in Sf9 cells denatured at a higher temperature than the nicotinic ace

33 tructure at high temperatures, Trp-cage cold denatures at 210 K into a compact, partially folded stat

34 elatively unstable three-helical bundle that denatures at pH <6.2 due to protonation of key histidine

35 ed us to assess the rate of folding from SDS-denatured bacteriorhodopsin (bRU) and provides accurate

36 with a sigmoidal increase in intermolecular (denatured) beta-sheets at a TOD (FTIR) of 72.4 degrees C

37 ldimethylglycine, and sodium dodecyl-sulfate denature both RANTES variants at low pH, whereas at neut

38 t bacteria and the probe (detection antibody-denatured BSA labelled with fluorophores) followed by th

39 3 degrees C, the natural collagenous part is denatured, but the C-terminal (GPP)7-NC2 remains intact.

40 aggregates that contain amyloid fibers when denatured, but there is little evidence that ex vivo cat

41 to participate in the refolding of proteins denatured by cytoplasmic stress, thus preventing their a

42 ding allow the chaperone to bind to proteins denatured by low pH, thereby protecting them from aggreg

43 Proteins were denatured by sodium dodecyl-sulphate (SDS) and precipita

44 s of the protein either extended by force or denatured by urea.

45 The extent of protein CSD shifting/denaturing can be tailored by acid identity and concentr

46 We find that MoS2 exhibits robust denaturing capability to HP35, with its secondary struct

47 Denatured class I HLA antibodies are common, but the ant

48 supposedly irrelevant antibodies recognizing denatured class I human leukocyte antigens (HLAs).

49 nstrated as a powerful vehicle for targeting denatured collagen (dColl) produced by disease or injury

50 whereas Fn is unusual in binding strongly to denatured collagen and more weakly to native collagen.

51 phonuclear cells (PMNs) ex vivo to adhere to denatured collagen in serum and polymyxin B independent

52 Severe collagen damage such as denatured collagen was also significantly less in the ir

53 ding sequence led to decreased Fn binding to denatured collagen.

54 not degrade types I, III, or IV collagen or denatured collagens in vitro.

55 fferent cleavage patterns between native and denatured collagens.

56 cus on targeting native collagens as well as denatured collagens.

57 t from studying enzyme conformations under a denaturing condition, it is highly informative to manipu

58 ta2m (hbeta2m) forms amyloid-like fibrils in denaturing conditions (e.g. pH2.5) in the absence of NaC

59 over, the acquisition of full MS scans under denaturing conditions allowed profiling of all abundant

60 ion auxiliaries that are removed under harsh denaturing conditions and have limited applicability.

61 We find that even under strongly denaturing conditions and in the absence of residual sec

62 ization method was developed using native or denaturing conditions by direct infusion into an Exactiv

63 re the behavior of proteins under native and denaturing conditions in the presence of NaCl, CsCl, and

64 This suggests that denaturing conditions mimic good solvents, whereby the p

65 quently, affinity purification is used under denaturing conditions to capture proteins conjugated wit

66 Comparing non-denaturing conditions to FDF-PAGE uncovered extensive se

67 ectric focusing (cIEF) is normally run under denaturing conditions using urea to expose any buried pr

68 or which the SETA reactivity increased under denaturing conditions were found to engage in tertiary p

69 noncyclic SST possess greater resistance to denaturing conditions with decreased monomer releasing p

70 cally disordered proteins, or proteins under denaturing conditions, and studies of amyloid aggregate

71 olded states can be populated under strongly denaturing conditions, but the ensemble which is relevan

72 Under mildly denaturing conditions, CysC forms inactive domain-swappe

73 Using denaturing conditions, the hemoglobin (Hb) tetramer diss

74 their activities under thermal and chemical denaturing conditions.

75 microscopy and biochemical assays under non-denaturing conditions.

76 e RNA interactions, and it is purified under denaturing conditions.

77 yaA has usually been extracted and stored in denaturing conditions.

78 proteins is isolated by centrifugation under denaturing conditions.

79 ntermediate states under a variety of mildly denaturing conditions.

80 proteins from E. coli cell lysates under non-denaturing conditions.

81 as required cofactors and/or unphysiological denaturing conditions.

82 tion), which are not detected under standard denaturing conditions.

83 pe VWF in the absence of shear but partially denaturing conditions.

84 tion of alpha-helices is found in chemically denatured configurations but such helices are absent und

85 depletion kinetics and significant loss from denatured controls.

86 and ATP, the more expanded and unstructured denatured cyclophilin A is not encapsulated but is expel

87 Similar mixing experiments with acid-denatured cytochrome c and the resulting folding during

88 ein isolate was prepared (CPI) and thermally denatured (DCPI).

89 I was the least denatured and VDCPI the most denatured, demonstrating the poorest solubility and foam

90 After extraction with non-denaturing detergents, we affinity-purified 785 endogeno

91 ators across various bottom-up assays (i.e., denaturing/digestion conditions).

92 DCPs prevent reassociation of denatured DNA strands: they make one of the two strands

93 ts renaturation of long stretches (>1 kb) of denatured dsDNA, in which Zn(2+)-tolerant intronic DNA p

94 developed in the past decades, all involving denaturing dsDNA and hybridizing fluorescent nucleic aci

95 erminal MDM2, and p53 DNA binding domain) or denatured due to solvent conditions (ubiquitin, apo hemo

96 cheese protein (WP) components that had been denatured during cooking of the whey.

97 in marine organisms against the detrimental denaturing effects of another naturally occurring osmoly

98 he Tau protein, that TMAO can counteract the denaturing effects of urea by inhibiting protein-urea pr

99 ith nMOMP/A8-35 had a higher ratio of Abs to denatured elementary bodies (EB) over live EB, recognize

100 to denatured rhodanese results in a compact denatured ensemble of the substrate protein.

101 These denaturing environments are thought to be good solvents

102 Therefore, typical denaturing environments cannot be classified as good sol

103 effects lead to conformational statistics in denaturing environments that are congruent with those of

104 then do generic protein sequences expand in denaturing environments?

105 ic species (either reaction side products or denatured enzymes, or both) on the cellulosic substrate

106 th misfolded client proteins, including heat-denatured enzymes, oxidized fibrinogen, oxidized LDL, an

107 In addition, dsRNA is difficult to denature even at elevated temperature, and the extinctio

108 The native and denaturing extraction protocols have captured 1109 orang

109 Treatment of SMVs with urea denatured extramembrane components of complex V, elimina

110 ggregation and resolubilization of thermally denatured firefly luciferase occurred independently of N

111 cAMP binding stabilizes the CBD-A against a denaturing force, and increases the folded state lifetim

112 erapeutic efficacy of a treatment based on a denatured form of the major allergen, phospholipase A2,

113 adhesin binds E. coli B LPS in its native or denatured form.

114 this problem, we established FDF-PAGE (fully-denaturing formaldehyde polyacrylamide gel electrophores

115 ng to a random distribution of unstructured, denatured forms, the results provide evidence for new co

116 Incorporation of extensively heated (heat-denatured) forms of milk and egg into the diets of child

117 ad of oxidative DNA cleavage is confirmed by denaturing gel electrophoresis and surface group pKa mea

118 variants within the gene were determined by denaturing gel electrophoresis or automated capillary-ba

119 l proteins were extracted and resolved using denaturing gel electrophoresis.

120 fy the functional form of TBCD and using non-denaturing gels and immunoblotting, we analyzed lysates

121 on immunoblots of blue-native gels, whereas denaturing gels and immunocytochemistry showed reduced c

122 sured for individual tRNA species using acid denaturing gels, or comparatively at the genome level us

123 ow charging levels were validated using acid denaturing gels.

124 rea-denatured lactate dehydrogenase and heat-denatured glucose-6-phosphate dehydrogenase.

125 tion method was evaluated to visually detect denatured glutathione peptides, utilizing gold nanoparti

126 Denaturing Gradient Gel Electrophoresis (DGGE) analysis

127 Plate counts, Denaturing Gradient Gel Electrophoresis (DGGE), short-ch

128 Denaturing gradient gel electrophoresis (DGGE), which co

129 phyton associated microbial consortium using denaturing gradient gel electrophoresis (DGGE).

130 Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-base

131 etermined by principal component analysis of denaturing gradient gel electrophoresis profiles was evi

132 and Fenton chemistry reactions catalyzed by denatured heme moieties bound to the RBC membrane.

133 mutations were detected by Sanger sequencing/denaturing high performance liquid chromatography (Ss/DH

134 as performed with polymerase chain reaction, denaturing high-performance liquid chromatography, and D

135 single antigen flow beads largely devoid of denatured HLA (iBeads) was also evaluated.

136 undiluted sera and SAB with greater than 30% denatured HLA (r = 0.40) and highest using diluted sera

137 using diluted sera and SAB with 30% or less denatured HLA (r = 0.86).

138 ing the HLA-A and HLA-B antigens targeted by denatured HLA antibodies from unacceptable antigens lowe

139 Denatured HLA antibodies were detected in 39% of the pat

140 Denatured HLA antibodies were identified according to ra

141 Antibody level, complement interference, and denatured HLA class I on SAB may all affect the clinical

142 Increasing levels of denatured HLA interfered with the detection of C1q bindi

143 single-antigen beads (SAB) is influenced by denatured HLA on SAB, antibody titre, and complement int

144 Conformational HLA and denatured HLA protein levels on SAB were determined usin

145 Denatured HLA was expressed as HC-10 binding to untreate

146 exhibited significantly altered mobility in denatured immunoblots of CaValpha2delta1 G1060I and CaVa

147 -exclusion chromatography, and nondenaturing/denaturing immunoblotting techniques.

148 In contrast, the unassembled RIDC3 monomers denature in approximately 30% THF and 50% iPrOH, indicat

149 DNA duplexes are effectively denatured in alkaline solutions; whereas, the denatured

150 Even thermostable RNA structures are often denatured in cells, highlighting the importance of cellu

151 Membrane proteins denatured in SDS can also be refolded by addition of NIS

152 difference highlights the importance of the denatured initial condition in folding experiments and l

153 de; PLGA) microparticles (MPs) encapsulating denatured insulin (key self-antigen in type 1 diabetes;

154 ponded to stimulation by B:11-23 peptide and denatured insulin protein were conclusively shown to rec

155 The strong and usually denaturing interaction between anionic surfactants (AS)

156 dimer dissociation and formation of the cold denatured intermediate occur without appreciable buildup

157 irst native melt conformation, dehydrate and denature into their final solid filament conformation.

158 Upon overexpression, SSA1 targets heat-denatured kinases toward degradation, which could enable

159 stem, HtpG enhanced native refolding of urea-denatured lactate dehydrogenase and heat-denatured gluco

160 ted the ability of Hsp22 and Hsp70 to refold denatured luciferase in vitro.

161 Defects in the reactivation of heat-denatured luciferase were also evident.

162 The heat-denatured malate dehydrogenase that did not refold by th

163 Non-denaturing mass spectrometry revealed AmpR to be a homot

164 a prerequisite before their analysis by the denaturing mass spectrometry technique.

165 Using non-denaturing mass spectrometry, we show that McjD in compl

166 Glu-meso-DAP-d-Ala-d-Ala, as assessed by non-denaturing mass spectrometry.

167 centres on reversible folding of chemically denatured membrane proteins.

168 roups based on the amount and degree of heat-denatured milk protein that they could tolerate.

169 fied Mtb ClpB/DnaK system reactivated a heat-denatured model substrate, but the DnaJ HPD mutants inhi

170 Mixing acid-denatured myoglobin with an aqueous ammonium acetate sol

171 lerate mass transfer and extract protein but denature myosin at high power inputs.

172 ically, peptides and proteins that have been denatured or degraded can lead to adverse patient reacti

173 n turnover or during stress when kinases are denatured or improperly folded.

174 uded from the vicinity of a protein, whereas denaturing osmolytes such as urea lead to protein unfold

175 tides may alter other interactions of partly denatured p53 with receptors.

176 DL with CHO-SR-B1 cells were analyzed by non-denaturing PAGE, size-exclusion chromatography, and the

177 values to enable the generation of different denatured particles.

178 nsition from the native, folded state to the denatured, partly unfolded state was revealed with the a

179 nscription inhibitor and confirmed by freeze-denatured platelets being incapable of replicating the D

180 Denaturing polyacrylamide gel electrophoresis (SDS-PAGE)

181 circular dichroism spectroscopy, native and denaturing polyacrylamide gel electrophoresis, and UV-vi

182 inked duplexes that were readily detected on denaturing polyacrylamide gels.

183 This effect was not impaired by a thermal denaturing process or blocked by the presence of anti-Fh

184 e of the double-stranded product followed by denaturing, produces a higher signal but the signal incr

185 ly found in short amyloidogenic peptides and denatured protein aggregates.

186 small reduction of the fraction of the heat denatured protein aggregates.

187 e no correlation between the conformation of denatured protein and the release of individual peptides

188 imeric protein and the lower affinity of the denatured protein compared with type II collagen.

189 When a denatured protein immersed in electrolyte is driven thro

190 Content of acidic monosaccharides and denatured protein increased with increasing FG extractio

191 This denatured protein matrixes could be in part preventing s

192 be used to detect the primary structure of a denatured protein molecule.

193 oxoid formulation consistently outperforms a denatured protein preparation in all of the metrics stud

194 ained by subtracting spectra of unheated and denatured protein solutions at different temperature-tim

195 digestion conditions and, by inference, from denatured protein structure.

196 The acid denatured protein was digested with pepsin and analyzed

197 were bound covalently to the native and heat denatured protein.

198 nally activated dissociation (CAD) MS of the denatured protein.

199 atrix with concentrated hydrochloric acid to denature proteins and allow the release of all arsenic s

200 compared under conditions typically used to denature proteins, i.e., thermal and chemical denaturati

201 lex interactions between gelatinized starch, denatured proteins and lipids.

202 Denatured proteins are first focused and separated in a

203 he same time, NMR results indicate that cold-denatured proteins are more expanded than heat-denatured

204 stages of protein refolding when chemically denatured proteins are transferred to native conditions.

205 predicted to remain above 3 kcal mol(-1) for denatured proteins as large as 900 residues.

206 rinsically disordered proteins (IDPs) and of denatured proteins based on nuclear magnetic resonance s

207 making it possible to release and refold SDS-denatured proteins by adding sufficient amounts of NIS,

208 nd in vitro it suppresses the aggregation of denatured proteins efficiently.

209 The thermally denatured proteins exposed different functional groups l

210 ment of this inherent dynamics in chemically denatured proteins is extremely challenging due to vario

211 onomous chaperone and associates with stress-denatured proteins to prevent them from aggregation simi

212 experimental values RG and RE For chemically denatured proteins we obtain mutual consistency in our i

213 The denatured proteins were used as substrate for tryptic hy

214 f this remarkable promiscuity by mapping two denatured proteins with very different conformational pr

215 ce data suitable for comparison with data of denatured proteins, we repeated the assignment in 7 M ur

216 natured proteins are more expanded than heat-denatured proteins.

217 rils in microorganisms and animals, and many denatured proteins.

218 can prevent the irreversible aggregation of denaturing proteins.

219 The native and denatured RBP fractions were hydrolyzed with papain and

220 e conjugate are compared to those derived by denaturing reversed phase liquid chromatography using an

221 functional and NMR analyses to show that the denatured RfaH refolds into the native state and that Rf

222 The fact that sonication-denatured rh-HGF had significantly lower uptake in U87MG

223 step the ATP-independent binding of DnaJ to denatured rhodanese results in a compact denatured ensem

224 , leads to a surprisingly large expansion of denatured rhodanese.

225 The results suggest that trypsin-hydrolyzed denatured rice bran albumin might be useful as a natural

226 lations demonstrate that the reversal in the denaturing role of pyridine is specific, and is related

227 he chemical reduction conditions employed to denature SA.

228 Using non-denaturing separations and mass spectrometry identificat

229 enatured in alkaline solutions; whereas, the denatured single-stranded DNA strands readily reform dup

230 Furthermore, the measurements of thermally denatured single-stranded DNA were carried out and the v

231 oup from myoglobin occurs as a result of the denaturing solution used.

232 espectively, when these ions are formed from denaturing solutions (water/methanol/acetic acid).

233 heir branched-cyclic topoisomers prepared in denaturing solvent conditions with and without sulfolane

234 he native state is no longer stable, such as denaturing solvents and high protein concentrations wher

235 However, urea does not completely denature some proteins, such as the tetrameric enzyme Er

236 salt-stimulated lipase (BSSL) and partially denaturing some of its proteins, potentially affecting i

237 ble surface area for unfolding, to probe the denatured state as a function of force, temperature, and

238 expose similar amounts of surface area, the denatured state at high temperature and low urea concent

239 le residues are generally not present in the denatured state ensemble (DSE) or in intrinsically disor

240 ed state and a heterogeneous fast-exchanging denatured state ensemble exhibiting residual secondary s

241 eGFP-promoted entropic stabilization of the denatured state ensemble of EnHD.

242 tion is observed upon transfer of the GdnHCl denatured state ensemble to native-like conditions.

243 s of both the native protein and a realistic denatured state ensemble.

244 the folding nucleus tend to interact in the denatured state in a transient manner and not simultaneo

245 Simulations over longer timescales, when the denatured state is less structured, do not show the anti

246 We thus find that the hot denatured state is more compact and richer in secondary

247 at of the guanidinium hydrochloride (GdnHCl) denatured state is present after ~27 mus of folding.

248 We find that the compaction of the denatured state is related to changing temperature as th

249 ee energy cost to form a trefoil knot in the denatured state of a polypeptide chain of 120 residues i

250 While it is widely appreciated that the denatured state of a protein is a heterogeneous conforma

251 ative and non-native structures in this acid-denatured state of ACBP, we rationalize the effects of s

252 However, whereas the compact denatured state of rhodanese is encapsulated efficiently

253 ensemble of structures representing an acid-denatured state of the 86-residue protein ACBP.

254 ative-state stability and compactness of the denatured state provides further evidence for native-lik

255 high denaturant indicates that any residual denatured state structure minimally affects the stabilit

256 should speed up refolding from the pressure-denatured state, if this hypothesis is correct.

257 richer in secondary structure than the cold denatured state, since water at lower temperatures can f

258 lso present in the nominally unfolded GdnHCl denatured state.

259 nce for native-like contact formation in the denatured state.

260 es to misligate to the heme in the protein's denatured state.

261 wn to be already lowly populated in the urea-denatured state.

262 ed (intermediate) --> irreversible unfolded (denatured) state, we study the thermal denaturation of h

263 ns (urea, force, and temperature) affect the denatured-state ensemble.

264 indicating that this part of the protein has denatured-state-level structure in the transition state.

265 find that while the urea- and force-induced denatured states expose similar amounts of surface area,

266 Whereas in other proteins the cold-denatured states have been found to be slightly more com

267 mpts to refold DsbB and GlpG from chemically denatured states into lipid membranes resulted in extens

268 this goal, we characterise the cold and hot denatured states of a protein by modelling NMR chemical

269 The characterization of denatured states of proteins is challenging because the

270 uctural differences between the hot and cold denatured states result in two alternative folding mecha

271 de series (AAXAA) as a model of unfolded and denatured states to examine the intrinsic propensities o

272 nd to be slightly more compact than the heat-denatured states, these two states occupy similar volume

273 te and was the same in both triple-helix and denatured states.

274 ted sensitively and specifically without any denaturing step.

275 release of components of the fuel (e.g., the denaturing substances) was also detected in the chamber

276 lters the conformational distribution of the denatured substrate protein rhodanese.

277 GrpE in the DnaK2-assisted refolding of the denatured substrates.

278 nhibits Cosmc-assisted in vitro refolding of denatured T-synthase.

279 template amyloid growth at physiological vs denaturing temperatures.

280 n detergents such as octylglucoside are more denaturing than long chain detergents such as dodecylmal

281 mobilized such that specific receptor forces denature the duplex and thus expose a blocked primer.

282 silica "cage", rendering them stable against denaturing thermal treatment and long-term ambient-tempe

283 embles of conformations corresponding to the denatured, transition, and native states in the folding

284 Following each CAPTR event, denatured ubiquitin ions (13+ to 6+) yield products that

285 tions (CAPTR) to reduce the charge states of denatured ubiquitin ions in the gas phase, and ion mobil

286 nditions is comparable to that of the GdnHCl denatured unfolded state.

287 ts suggest that other proteins might also be denatured upon adsorption onto graphene, such that the g

288 Glutathione was denatured using a 70 degrees C water bath to create an a

289 ing the oxygenated form of rHb Kirklareli to denature very rapidly under physiological conditions.

290 Following stringent denaturing washes, the resulting covalently linked prote

291 No separate aggregation of kappa-casein/denatured whey protein complexes or kappa-casein deplete

292 n and the concentrations of kappa-casein and denatured whey protein in the serum, and a reduction in

293 on were characterised and loaded into a heat-denatured whey protein isolate (WPI) solution which was

294 347nm were fabricated and added into a heat-denatured whey protein solution.

295 f total whey protein, partial association of denatured whey protein with the casein micelle, an incre

296 ation to pH 5.4, the serum phase caseins and denatured whey proteins partially reassociated with the

297 At pH below 5.4 the caseins and denatured whey proteins rapidly aggregated.

298 s resulted in different levels of casein and denatured whey proteins to be distributed between the co

299 sical and probiotic yogurt the totals of non-denatured whey proteins were 92.31 and 91.03%.

300 Hydrolysis of denatured WPI favoured the generation of higher levels o