ライフサイエンスコーパス: denaturing

1 tic pressure inside the NMR sample cell from denaturing (2.5 kbar) to native (1 bar) conditions.

2 ure proteins, the molecular mechanism of its denaturing ability is still a subject of considerable de

3 Because of its protein-denaturing ability, urea has played a pivotal role in th

4 e adapted and used a highly stringent Tandem Denaturing Affinity Purification (TDAP) method, coupled

5 Apple PPO was thermolabile, denaturing after 10min at 70 degrees C.

6 NA-capsids are based on two-dimensional, non-denaturing agarose gel electrophoresis, followed by stru

7 3 activity on full-length VWF arises without denaturing agent as evidenced by FCS and gel-based multi

8 We show that denaturing agents can alter digestion by reducing protea

9 Use of alkylureas as denaturing agents in capillary electrophoresis and cIEF

10 proaches studying the effect of proteases or denaturing agents on the physicochemical properties of P

11 ium, Tris), magnesium ions and commonly used denaturing agents such as formamide and DMSO.

12 ng amyloid fibrils formed in the presence of denaturing agents that significantly alter the folding s

13 l denaturation, complex coupling between two denaturing agents, and differential scanning calorimetry

14 ilibrium unfolding, complex coupling between denaturing agents, characteristic DSC thermogram, gradua

15 high inherent susceptibility to reducing and denaturing agents, it is very stable upon immobilization

16 3 microL) with 1.5 microg of trypsin without denaturing agents.

17 from its structured state in the absence of denaturing agents.

18 t during demineralization or with the use of denaturing agents.

19 bolishes the chaperone-like activity against denaturing alcohol dehydrogenase.

20 nto peptides by processes that use salts for denaturing and buffering purposes.

21 les cause structural changes including local denaturing and compaction to DNA under mild conditions.

22 distributions of globular proteins under non-denaturing and denaturing conditions.

23 acid and aspartic acid over time under both denaturing and native conditions at pH 8.2, we found tha

24 Using both denaturing and native gel analysis, we have identified n

25 Immunoprecipitation, cross-linking, and denaturing and native PAGE analyses of Gal80 in vitro an

26 eriments on ubiquitin ions ionized from both denaturing and native solution conditions suggest that r

27 the ability to run the samples in different denaturing and nondenaturing buffer allows defining true

28 0B after oxidation of its thiol groups under denaturing and nondenaturing conditions, respectively.

29 nstrated across pH 3.8-7.0 muIPGs using both denaturing and nondenaturing conditions, without the add

30 In silico analyses, denaturing and nondenaturing gel Western blot, and immun

31 ent PRDC dimers are highly stable under both denaturing and reducing conditions.

32 ing and dissociation under native, partially denaturing, and fully unfolded conditions indicate that

33 peanut, and pistachio flours using three non-denaturing aqueous (phosphate, borate, and carbonate) bu

34 single and interactive effects of three non-denaturing aqueous (phosphate, borate, and carbonate) bu

35 A two-dimension matrix of pH and denaturing buffer conditions was screened to optimize na

36 The extent of protein CSD shifting/denaturing can be tailored by acid identity and concentr

37 We find that MoS2 exhibits robust denaturing capability to HP35, with its secondary struct

38 or large populations may be discovered using denaturing capillary electrophoresis (DCE).

39 dinarily would decompose as a consequence of denaturing chromatographic conditions typically used for

40 ve, ATP-independent chaperones that can bind denaturing client proteins to prevent their irreversible

41 n, and hence imply a reduced risk of protein denaturing compared with gold films or extended surfaces

42 he polymer properties of Gln repeats even in denaturing concentrations of GdnHCl.

43 t from studying enzyme conformations under a denaturing condition, it is highly informative to manipu

44 ta2m (hbeta2m) forms amyloid-like fibrils in denaturing conditions (e.g. pH2.5) in the absence of NaC

45 trast, globular proteins unfolded under less denaturing conditions (via mutations, or transiently unf

46 over, the acquisition of full MS scans under denaturing conditions allowed profiling of all abundant

47 oligoheparin-AT interaction under partially denaturing conditions allowed the conformer specificity

48 immunoprecipitation under nondenaturing and denaturing conditions and detected with antibodies for m

49 ion auxiliaries that are removed under harsh denaturing conditions and have limited applicability.

50 We find that even under strongly denaturing conditions and in the absence of residual sec

51 and purified a recombinant form of p33 under denaturing conditions and optimized conditions for the r

52 ic activity over the same RNAs, purified via denaturing conditions and refolded.

53 mentary procedures, which use strong protein denaturing conditions and yield highly purified core and

54 ization method was developed using native or denaturing conditions by direct infusion into an Exactiv

55 We find that mild denaturing conditions can affect the sensitive balance b

56 g those obtained under traditional native or denaturing conditions can be reversibly obtained simply

57 ha-synuclein isolated and analysed under non-denaturing conditions from neuronal and non-neuronal cel

58 r contacts has been studied under a range of denaturing conditions in single domains of the immunoglo

59 re the behavior of proteins under native and denaturing conditions in the presence of NaCl, CsCl, and

60 ion state predicted to dominate under highly denaturing conditions is only possible by simulation.

61 This suggests that denaturing conditions mimic good solvents, whereby the p

62 Thus, 30% SDS and 3% SDS + 8 M urea are the denaturing conditions of choice to study maximally unfol

63 ORF2 purified from bacteria under denaturing conditions preferentially interacted with dou

64 The sizes of unfolded proteins under highly denaturing conditions scale as N(0.59) with chain length

65 By purifying under denaturing conditions this method not only reduces the r

66 e of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial

67 quently, affinity purification is used under denaturing conditions to capture proteins conjugated wit

68 Comparing non-denaturing conditions to FDF-PAGE uncovered extensive se

69 ectric focusing (cIEF) is normally run under denaturing conditions using urea to expose any buried pr

70 or which the SETA reactivity increased under denaturing conditions were found to engage in tertiary p

71 ints imposed by the gel pores such that more denaturing conditions were required within the gel than

72 noncyclic SST possess greater resistance to denaturing conditions with decreased monomer releasing p

73 d when the samples were loaded in moderately denaturing conditions with multiple loading-washing cycl

74 emperature and the values at 55.0 degrees C (denaturing conditions) are very similar to those at 25.0

75 cally disordered proteins, or proteins under denaturing conditions, and studies of amyloid aggregate

76 common instrumentation, is carried out under denaturing conditions, and takes less than 30min.

77 olded states can be populated under strongly denaturing conditions, but the ensemble which is relevan

78 Under mildly denaturing conditions, CysC forms inactive domain-swappe

79 onjugates by purification of 6His-SUMO under denaturing conditions, followed by western blotting for

80 These techniques, generally run under denaturing conditions, have proven to be only marginally

81 Under denaturing conditions, limited membrane digestion of bov

82 tive conditions at pH 8.2, we found that, at denaturing conditions, succinimide at light chain residu

83 We also found that, under denaturing conditions, succinimide hydrolyzed at an isoa

84 e more stable than the native ensemble under denaturing conditions, such as high temperature.

85 microtubules by paclitaxel, laulimalide, or denaturing conditions, suggesting irreversible binding o

86 Following purification under denaturing conditions, SUMOylated proteins were identifi

87 After exposure to denaturing conditions, the C-terminal fragment of LPL re

88 Surprisingly, even under denaturing conditions, the energy landscape remains high

89 Using denaturing conditions, the hemoglobin (Hb) tetramer diss

90 inguishable from that of amyloid grown under denaturing conditions, where the C-terminal alpha-helica

91 nditions and solvent-exposed under partially denaturing conditions, whereas the region 95-105 was sol

92 conditions and aggregation experiments under denaturing conditions.

93 rmation by MR in aqueous solutions under non-denaturing conditions.

94 yet durably retain their targets even under denaturing conditions.

95 aled a single conformational ensemble at all denaturing conditions.

96 dopt a completely disordered structure under denaturing conditions.

97 as required cofactors and/or unphysiological denaturing conditions.

98 no acids to promote residual structure under denaturing conditions.

99 +) were restored to inactive PS under mildly denaturing conditions.

100 f globular proteins under non-denaturing and denaturing conditions.

101 enhances the stability of that enzyme under denaturing conditions.

102 urant concentrations and deviated under less denaturing conditions.

103 ted by monitoring the change in A(230) under denaturing conditions.

104 criteria, although they were purified under denaturing conditions.

105 iols, amines, and acids in high purity under denaturing conditions.

106 ility to modulate the structure of TTR under denaturing conditions.

107 sulfhydryl (-SH) content on applying mildly denaturing conditions.

108 tion), which are not detected under standard denaturing conditions.

109 essential to retain triplex structure under denaturing conditions.

110 pe VWF in the absence of shear but partially denaturing conditions.

111 y stable at high temperatures and even under denaturing conditions.

112 tion methods that subject proteins to harsh, denaturing conditions.

113 topes was reduced upon exposure to partially denaturing conditions.

114 microscopy and biochemical assays under non-denaturing conditions.

115 their activities under thermal and chemical denaturing conditions.

116 e RNA interactions, and it is purified under denaturing conditions.

117 yaA has usually been extracted and stored in denaturing conditions.

118 proteins is isolated by centrifugation under denaturing conditions.

119 ntermediate states under a variety of mildly denaturing conditions.

120 proteins from E. coli cell lysates under non-denaturing conditions.

121 aggregates they formed, as revealed by semi-denaturing detergent agarose gel electrophoresis.

122 between KS and a protein's resistance to the denaturing detergent SDS, we show here the application o

123 n detergents such as heptylthioglucoside and denaturing detergents such as SDS.

124 After extraction with non-denaturing detergents, we affinity-purified 785 endogeno

125 ators across various bottom-up assays (i.e., denaturing/digestion conditions).

126 to online mass spectrometry or result in the denaturing dissociation of conjugated HC and LC during c

127 site induces directed loop migration toward denaturing domains, a phenomenon that merges destabilizi

128 developed in the past decades, all involving denaturing dsDNA and hybridizing fluorescent nucleic aci

129 n binding on the channel surface and protein denaturing during device assembly.

130 mbrane, provides a rationale for the general denaturing effect of the CL surface and the large-scale

131 nd trimethylamine N-oxide can counteract the denaturing effect of urea.

132 ears sufficient to explain the thermodynamic denaturing effect of urea.

133 in marine organisms against the detrimental denaturing effects of another naturally occurring osmoly

134 he Tau protein, that TMAO can counteract the denaturing effects of urea by inhibiting protein-urea pr

135 wed by separating the cleavage products on a denaturing electrophoresis gel to identify the protein-b

136 ion under excursions in temperature, pH, and denaturing environment.

137 These denaturing environments are thought to be good solvents

138 Therefore, typical denaturing environments cannot be classified as good sol

139 effects lead to conformational statistics in denaturing environments that are congruent with those of

140 then do generic protein sequences expand in denaturing environments?

141 extent that it accumulates even under mildly denaturing equilibrium conditions.

142 simulation in water, we found six transient denaturing events near the melting temperature (323 and

143 pe, which is consistent with the recent urea denaturing experiment.

144 tion at 37 degrees C was detected in thermal denaturing experiments.

145 The native and denaturing extraction protocols have captured 1109 orang

146 cAMP binding stabilizes the CBD-A against a denaturing force, and increases the folded state lifetim

147 this problem, we established FDF-PAGE (fully-denaturing formaldehyde polyacrylamide gel electrophores

148 on of the labeled small RNAs is performed by denaturing gel electrophoresis and autoradiography or ph

149 ad of oxidative DNA cleavage is confirmed by denaturing gel electrophoresis and surface group pKa mea

150 ulation of RNA molecules is separated by non-denaturing gel electrophoresis and the conformers are in

151 xes with linear RNA targets were detected by denaturing gel electrophoresis and were found to be more

152 involves the separation of RNA molecules by denaturing gel electrophoresis followed by transfer and

153 Following EMSA, denaturing gel electrophoresis is implemented to provide

154 variants within the gene were determined by denaturing gel electrophoresis or automated capillary-ba

155 lpha-syn to these standards using native and denaturing gel electrophoresis techniques, size-exclusio

156 ll lysates and intact cells and by using non-denaturing gel electrophoresis without cross-linking, th

157 on the analysis of RNA products separated by denaturing gel electrophoresis, Nsp15 has been reported

158 Here, we present non-denaturing gel electrophoresis, UV melting, and circular

159 f the synapse of two site Is observed by non-denaturing gel electrophoresis.

160 l proteins were extracted and resolved using denaturing gel electrophoresis.

161 spectrometry at approximately 50 kDa and by denaturing gel filtration at approximately 50-61 kDa, wh

162 CSalpha to retain its 32P throughout the SDS denaturing gel process was unique over the entire mitoch

163 fy the functional form of TBCD and using non-denaturing gels and immunoblotting, we analyzed lysates

164 on immunoblots of blue-native gels, whereas denaturing gels and immunocytochemistry showed reduced c

165 Filtration assays and non-denaturing gels demonstrated that Abeta oligomers cross-

166 and of overexpression, sample heating and/or denaturing gels for cell culture and tissue studies.

167 sured for individual tRNA species using acid denaturing gels, or comparatively at the genome level us

168 resulting in a significant mobility shift on denaturing gels.

169 ow charging levels were validated using acid denaturing gels.

170 ring transition-metal ions, inducing protein denaturing, generating air bubbles or making pores in me

171 ers the melting properties of DNA molecules, denaturing gradient gel blots (DGG blots), a gene scanni

172 Denaturing Gradient Gel Electrophoresis (DGGE) analysis

173 munities and T. versicolor was studied using denaturing gradient gel electrophoresis (DGGE) and the c

174 ntial isolation and eubacterium-specific PCR-denaturing gradient gel electrophoresis (DGGE) in conjun

175 Denaturing gradient gel electrophoresis (DGGE) of the cl

176 t bacterial community structure derived from denaturing gradient gel electrophoresis (DGGE) shifted m

177 Plate counts, Denaturing Gradient Gel Electrophoresis (DGGE), short-ch

178 Denaturing gradient gel electrophoresis (DGGE), which co

179 nd sequencing or community fingerprinting by denaturing gradient gel electrophoresis (DGGE).

180 of a cultivation-independent approach called denaturing gradient gel electrophoresis (DGGE).

181 phyton associated microbial consortium using denaturing gradient gel electrophoresis (DGGE).

182 Using PCR-based denaturing gradient gel electrophoresis analyses of oral

183 Gut microbiota profiling by denaturing gradient gel electrophoresis and 454/FLX-base

184 submitted to polymerase chain reaction(PCR)-denaturing gradient gel electrophoresis and real-time qu

185 etermined by principal component analysis of denaturing gradient gel electrophoresis profiles was evi

186 dant with ITS lineage membership (defined by denaturing gradient gel electrophoresis screening), yet

187 Denaturing gradient gel electrophoresis similarly reveal

188 s of the biocides applied, by using cloning, denaturing gradient gel electrophoresis, and culture-dep

189 traviolet hypochromicity methods, as well as denaturing gradient gel electrophoretic methods.

190 mutations were detected by Sanger sequencing/denaturing high performance liquid chromatography (Ss/DH

191 Using denaturing high performance liquid chromatography, massi

192 Denaturing high-performance liquid chromatography (DHPLC

193 Using denaturing high-performance liquid chromatography (DHPLC

194 PCR-amplified samples were pre-screened by denaturing high-performance liquid chromatography and co

195 A (NaV1.5, LQTS type 3) were performed using denaturing high-performance liquid chromatography and di

196 xcluding acute promyelocytic leukemia, using denaturing high-performance liquid chromatography and di

197 riations in the ATM gene by a combination of denaturing high-performance liquid chromatography and di

198 xon, followed by heteroduplex analysis using denaturing high-performance liquid chromatography and di

199 Using denaturing high-performance liquid chromatography and di

200 KIT in exon 17 (mutKIT17) and 8 (mutKIT8) by denaturing high-performance liquid chromatography and di

201 egions were PCR-amplified and analyzed using denaturing high-performance liquid chromatography and DN

202 obtained and screened for SCN5A mutations by denaturing high-performance liquid chromatography and se

203 samples from 470 cases of isolated CHD using denaturing high-performance liquid chromatography and se

204 and FBXW7 were screened by a combination of denaturing high-performance liquid chromatography and se

205 ATM status was analyzed by denaturing high-performance liquid chromatography and wa

206 Denaturing high-performance liquid chromatography was us

207 Denaturing high-performance liquid chromatography was us

208 as performed with polymerase chain reaction, denaturing high-performance liquid chromatography, and d

209 Using polymerase chain reaction, denaturing high-performance liquid chromatography, and D

210 n of ANKRD1 using polymerase chain reaction, denaturing high-performance liquid chromatography, and d

211 Using polymerase chain reaction, denaturing high-performance liquid chromatography, and d

212 Using polymerase chain reaction, denaturing high-performance liquid chromatography, and d

213 as performed with polymerase chain reaction, denaturing high-performance liquid chromatography, and D

214 ombination of ABCR400 genotyping microarray, denaturing high-performance liquid chromatography, and h

215 sets of mutation accumulation lines by using denaturing high-performance liquid chromatography.

216 3 chip and the Surveyor DNA endonuclease and denaturing high-performance liquid chromatography.

217 This analysis was based on a combination of denaturing HPLC mutation screening of all exons of the p

218 A cohort of 202 probands was screened by denaturing HPLC, followed by direct sequencing using a c

219 -exclusion chromatography, and nondenaturing/denaturing immunoblotting techniques.

220 d by cutting the protease site with trypsin, denaturing in guanidine hydrochloride to disrupt the com

221 riate cellular locations, and keep them from denaturing in the face of normal and pathological stimul

222 ogenesis may occur directly from assembly of denaturing insulin monomers rather than by successive tr

223 The strong and usually denaturing interaction between anionic surfactants (AS)

224 5)I]Cry1A toxins under native (dot blot) and denaturing (ligand blot) conditions.

225 This is achieved by introducing a denaturing lysis step (in the presence of sodium dodecyl

226 Non-denaturing mass spectrometry analysis and analytical ult

227 Non-denaturing mass spectrometry revealed AmpR to be a homot

228 a prerequisite before their analysis by the denaturing mass spectrometry technique.

229 Using non-denaturing mass spectrometry, we show that McjD in compl

230 Glu-meso-DAP-d-Ala-d-Ala, as assessed by non-denaturing mass spectrometry.

231 lude that large complex RNAs prepared by non-denaturing means form a homogeneous population and are c

232 lytically more active than those prepared by denaturing methods and subsequent refolding; this facile

233 the peptides, a phenomenon akin to the cold denaturing of some proteins.

234 folding process, indicating that the thermal denaturing of the single mutation is robust and reproduc

235 -syn expressed in E. coli and purified under denaturing or nondenaturing conditions, whether as a fre

236 Urea is known as a common denaturing osmolyte that affects protein function by des

237 g osmolyte trimethylamine-N-oxide (TMAO) and denaturing osmolyte urea for the case of alpha-synuclein

238 Urea, a denaturing osmolyte, shifts the equilibrium toward U; tr

239 uded from the vicinity of a protein, whereas denaturing osmolytes such as urea lead to protein unfold

240 the model correctly predicts that protecting/denaturing osmolytes will be preferentially excluded/acc

241 lex DNA with either intercalator followed by denaturing PAGE allows the detection of mismatches in ol

242 o1, we performed chemical cross-linking, non-denaturing PAGE, and electromobility shift assays, which

243 DL with CHO-SR-B1 cells were analyzed by non-denaturing PAGE, size-exclusion chromatography, and the

244 We generated various hybrids by denaturing pairs of enzymes in 1 M guanidinium chloride

245 Denaturing polyacrylamide gel electrophoresis (SDS-PAGE)

246 Co-affinity purification, non-denaturing polyacrylamide gel electrophoresis and bis(ma

247 sulting products are size-fractionated using denaturing polyacrylamide gel electrophoresis and visual

248 uniquely 3'-radiolabeled DNA substrates and denaturing polyacrylamide gel electrophoresis to provide

249 circular dichroism spectroscopy, native and denaturing polyacrylamide gel electrophoresis, and UV-vi

250 assay (RIKA), a kinase is copolymerized in a denaturing polyacrylamide gel used to resolve a tissue o

251 inked duplexes that were readily detected on denaturing polyacrylamide gels.

252 However, the mechanism behind its denaturing power is still not well understood.

253 This effect was not impaired by a thermal denaturing process or blocked by the presence of anti-Fh

254 e of the double-stranded product followed by denaturing, produces a higher signal but the signal incr

255 micelles are the most common choice, but the denaturing properties make them unsatisfactory for studi

256 ysis, and immunoprecipitation as well as non-denaturing protein affinity purification.

257 duced cell damage by binding and maintaining denaturing proteins in a folding-competent state.

258 independent chaperones to bind and stabilize denaturing proteins that can be later reactivated by ATP

259 bility to behave as an unfoldase, repeatedly denaturing proteins upon binding, which in turn can free

260 These findings suggest that, in denaturing proteins, urea (and perhaps other denaturants

261 urea has been commonly used as an agent for denaturing proteins.

262 one that prevents nonspecific aggregation of denaturing proteins.

263 can prevent the irreversible aggregation of denaturing proteins.

264 n the pH and temperature and the presence of denaturing reagents.

265 -micellar and sub-denaturing to micellar and denaturing, revealed multiple complexes of UBI and SDS o

266 e conjugate are compared to those derived by denaturing reversed phase liquid chromatography using an

267 lations demonstrate that the reversal in the denaturing role of pyridine is specific, and is related

268 formed complexes as detected by reducing and denaturing SDS-PAGE.

269 Upon partial denaturing, seeds consisting of 3-4 monomers quickly app

270 Using non-denaturing separations and mass spectrometry identificat

271 Our thermal denaturing simulations at 400-500 K with both the OPLSAA

272 fied transition states from high-temperature denaturing simulations.

273 oup from myoglobin occurs as a result of the denaturing solution used.

274 espectively, when these ions are formed from denaturing solutions (water/methanol/acetic acid).

275 reater than those obtained from conventional denaturing solutions consisting of water/methanol/acid,

276 ces are less sensitive to solvent quality in denaturing solutions of GdnHCl than Gly-rich sequences.

277 arging appear similar to those obtained from denaturing solutions where charging beyond the total num

278 supercharging from native solutions and from denaturing solutions.

279 heir branched-cyclic topoisomers prepared in denaturing solvent conditions with and without sulfolane

280 when electrospraying unfolded proteins in a denaturing solvent.

281 he native state is no longer stable, such as denaturing solvents and high protein concentrations wher

282 salt-stimulated lipase (BSSL) and partially denaturing some of its proteins, potentially affecting i

283 ve effects on filament assembly: positive by denaturing ssDNA secondary structure, and negative by co

284 At the initial heat-denaturing step and during the PCR sequence, the 3'-prot

285 ted sensitively and specifically without any denaturing step.

286 or dsDNA and eliminates the need for thermal denaturing steps typically required for DNA hybridizatio

287 in synthesis or during cellular responses to denaturing stress.

288 rganic ions and urea, it imposes osmotic and denaturing stresses on bacterial cells.

289 release of components of the fuel (e.g., the denaturing substances) was also detected in the chamber

290 Here, we show that the denaturing temperatures of both hen egg white lysozyme a

291 template amyloid growth at physiological vs denaturing temperatures.

292 n detergents such as octylglucoside are more denaturing than long chain detergents such as dodecylmal

293 eavy chain on an antibody were eliminated by denaturing the protein, revealing that structural elemen

294 free energy of binding by about 30% without denaturing the protein, suggesting that hydrophobic forc

295 silica "cage", rendering them stable against denaturing thermal treatment and long-term ambient-tempe

296 ons of SDS ranging from sub-micellar and sub-denaturing to micellar and denaturing, revealed multiple

297 emains unclear whether in the absence of any denaturing treatment, PrP-lipid interaction is sufficien

298 ational epitopes remain functional following denaturing treatments and indicate that cellular PrP and

299 Following stringent denaturing washes, the resulting covalently linked prote

300 chloride IL was discovered to be capable of denaturing wool and was determined to be the most effect