ライフサイエンスコーパス: dense granule

1 on of only one major platelet organelle, the dense granule.

2 between proteins secreted from rhoptries and dense granules.

3 f the murine ashen (Rab27a) gene in platelet-dense granules.

4 utive secretory vesicles of tachyzoites, the dense granules.

5 cifically regulates the contents of platelet-dense granules.

6 C) transporter, ABCC4, functions in platelet-dense granules.

7 mal system, such as melanosomes and platelet dense granules.

8 organelles, such as melanosomes and platelet dense granules.

9 organelles such as melanosomes and platelet-dense granules.

10 ined to melanosomes, lysosomes, and platelet dense granules.

11 organelles such as melanosomes and platelet dense granules.

12 organelles such as melanosomes and platelet-dense granules.

13 alized to the Golgi of T. gondii, but not to dense granules.

14 t of the role of TgARF1 in release of intact dense granules.

15 esis of melanosomes, lysosomes, and platelet dense granules.

16 ncy, reflecting the malformation of platelet dense granules.

17 es and a reduction in the number of platelet dense granules.

18 tion of melanosomes, lysosomes, and platelet dense granules.

19 , beta-lactamase, that localizes to parasite dense granules.

20 nous dense granule protein GRA4 localized to dense granules.

21 Toxoplasma gondii are derived from parasite dense granules.

22 rofilaments, some mitochondria, vacuoles and dense granules.

23 man HPS patients in melanosomes and platelet-dense granules.

24 ultrastructurally as intracellular electron-dense granules.

25 lted in a marked dose-dependent secretion of dense granules.

26 BCC4), which facilitates ADP accumulation in dense granules.

27 tein-3 are necessary for normal transport to dense granules.

28 ansky-Pudlak syndrome, ruby-eye, which lacks dense granules.

29 eted effectors rhoptry 16 kinase (ROP16) and dense granule 15 (GRA15) activate the JAK-STAT3/6 and NF

30 Lysosomal and platelet dense granule abnormalities, including hyposecretion of

31 Contents released from dense granules after platelet activation promote coagula

32 Dense granules also are targeted by antiplatelet agents

33 ral role in amplifying platelet aggregation, dense granule and alpha-granule secretion, P-selectin ex

34 ated transport vesicles, and microdomains of dense granule and endosomal membranes.

35 herefore, the molecular understanding of the dense granule and its biogenesis is of vital importance.

36 were delivered quantitatively into parasite dense granules and efficiently secreted into the vacuola

37 secrete contents of both alpha-granules and dense granules and generate thromboxane A2 (TXA2), but p

38 inorganic phosphate, is present in platelet dense granules and is secreted on platelet activation.

39 lving abnormalities of melanosomes, platelet dense granules and lysosomes.

40 ian organelles such as melanosomes, platelet dense granules and lysosomes.

41 result from defects of melanosomes, platelet dense granules and lysosomes.

42 ytoplasmic organelles: melanosomes, platelet dense granules and lysosomes.

43 organelles, including melanosomes, platelet dense granules and lysosomes.

44 cretions of another class of organelles, the dense granules and osmiophilic bodies, are proposed to b

45 The synthesis of dense granules and other lysosome-related organelles is

46 The mass-dense granules and the contractile vacuole appeared to c

47 primarily to the Golgi, although staining of dense granules and the intravacuolar network was also de

48 Targeting of secreted proteins to T. gondii dense granules and the plasma membrane uses general mech

49 s was reduced secretion from alpha-granules, dense granules, and lysosomes following CRP stimulation.

50 cytoplasmic organelles-melanosomes, platelet-dense granules, and lysosomes.

51 cytoplasmic organelles-melanosomes, platelet-dense granules, and lysosomes.

52 sma apical secretory organelles (micronemes, dense granules, and rhoptries) play key roles in host ce

53 te organelles, namely micronemes, rhoptries, dense granules, and the apicoplast.

54 appearance, contained abundant intracellular dense granules, and were surrounded by a less-dense matr

55 iation to mature Mks synthesizing alpha- and dense-granules, and forming PPTs without exogenous throm

56 mature protease is concentrated in merozoite dense granules, apical secretory organelles involved in

57 s, CgA-expressing A35C cells showed electron-dense granules approximately 180-220 nm in diameter, and

58 Lysosomes, melanosomes and platelet-dense granules are abnormal in the mouse hypopigmentatio

59 Hermansky-Pudlak syndrome in which platelet dense granules are absent.

60 Dense granules are important in platelet aggregation to

61 der the cytoplasmic membrane, a region where dense granules are known to migrate after maturation.

62 Platelet dense granules are lysosome-related organelles which con

63 Platelet dense granules are members of a family of tissue-specifi

64 Toxoplasma gondii dense granules are morphologically similar to dense matr

65 Thus, essentially normal numbers of platelet dense granules are produced but the granule interiors ar

66 rthermore, the presence of numerous electron-dense granules around the phagosomes indicated that neut

67 ne nucleotides though near-normal numbers of dense granules as enumerated by the dense granule-specif

68 organelles such as melanosomes and platelet dense granules as well as to neurotransmitter vesicles.

69 MRP4 redistributed cAMP from the cytosol to dense granules, as confirmed by increased vasodilator-st

70 This work sheds light on the biogenesis of dense granules at the molecular level and opens the poss

71 ranules, MHC class II compartments, platelet-dense granules, basophil granules, azurophil granules, a

72 arly endosomes as MKs mature or functions in dense granule biogenesis directly from early endosomes,

73 rome is now known to be related to defective dense granule biogenesis due to mutations in any of >/=9

74 (MEG-01) as a model system for the study of dense granule biogenesis using a variety of cell biology

75 w that early endosomes play a direct role in dense granule biogenesis.

76 sed Golgi staining and increased delivery to dense granules but blocked delivery to the intravacuolar

77 MP-3, whereas rVAMP-8 inhibited secretion of dense granules but not alpha granules.

78 dom is the presence of perinuclear, electron-dense granules called nuage.

79 rucial for the fusion of vesicles containing dense granule cargo with the maturing organelle.

80 Ap3A is a platelet-dense granule component released into the extracellular

81 n background have greatly reduced amounts of dense granule components such as serotonin and adenine n

82 he effects of the ashen mutation on platelet-dense granule components, platelet aggregation, and blee

83 g that sporozoites have a different electron-dense-granule composition, we have now found that sporoz

84 g that the impairment is secondary to absent dense granule content release.

85 ha-granule proteins and reduced secretion of dense granule contents critical for platelet activation.

86 e secretagogue which promotes the release of dense granule contents; (2) colocalization with ACTH, an

87 we used SLC35D3, mutation of which causes a dense granule defect in mice, to show that early endosom

88 The platelet-dense granule defect is rescued in BAC transgenic mice c

89 tion promote coagulation and hemostasis, and dense granule defects such as those seen in Hermansky-Pu

90 reased bleeding time in mice and humans with dense granule deficiency.

91 ) who are specifically deficient in platelet dense granules (delta-SPD) have suggested a role for den

92 Centriolar satellites are numerous electron-dense granules dispersed around the centrosome.

93 ing diathesis due to the absence of platelet dense granules, displays extensive locus heterogeneity.

94 equired for efficient secretion of alpha and dense granules downstream of G(13) and G(q).

95 ) platelets demonstrated a partial defect in dense granule exocytosis and impaired aggregation.

96 unc18c with syntaxin enhanced Ca2+-triggered dense granule exocytosis in permeabilized cells.

97 e to release of agonists other than ADP from dense granules, experiments were performed on murine pla

98 sive bleeding, but little is known about how dense granules form in megakaryocytes (MKs).

99 that SLC35D3 is either delivered to nascent dense granules from contiguous early endosomes as MKs ma

100 The composition of these crystals and the dense granules from which they are derived has remained

101 Platelets lacking ABCC4 have unchanged dense-granule function, number, and volume, but harbor a

102 Serotonin, an abundant component of platelet-dense granules, has an Mr of 176, and fibrinogen isolate

103 l portion of the protein constituents of the dense granules have been identified, and little is known

104 side the parasite that are distinct from the dense granules; however, in the encysted bradyzoite stag

105 Pactolus protein is held within the cell in dense granules in a highly glycosylated form.

106 tron microscopy revealed a reduced number of dense granules in affected patients platelets, correlati

107 tivity localized to the cytoplasmic electron-dense granules in ASPS.

108 nalysis revealed a higher number of electron-dense granules in Ctr2(-/-) mast cells than in wild-type

109 strated a combination of ASPS-like features (dense granules in four cases, rhomboid crystals in two c

110 Rab27b localizes to alpha and dense granules in megakaryocytes.

111 ckout mice showed increased numbers of small dense granules in the granular layer with few or no surr

112 Platelet aggregation and secretion from dense granules induced by CRP and thrombin was slightly

113 TgPI-1(41) are secreted constitutively from dense granules into the excreted/secreted antigen fracti

114 PI1 isoforms, both of which are secreted via dense granules into the parasitophorous vacuole shortly

115 The dense granule localization of the single pass transmembr

116 a length similar to that of GRA4 resulted in dense granule localization, whereas lengthening the GRA4

117 w demonstrate that NO inhibits exocytosis of dense granules, lysosomal granules, and alpha-granules f

118 the related subcellular organelles platelet dense granules, lysosomes, and melanosomes.

119 We compared the secretion of the endogenous dense granule marker GRA3 in Toxoplasma gondii with the

120 platelet membrane, and provide evidence that dense granules may be a major source of ADP which can co

121 uggest a close relationship between platelet dense granules, melanosomes of melanocytes and secretory

122 ndent fashion, inducing release of alpha and dense granules, membrane alterations, aggregation, and f

123 elet releasates (the 'secretome'), alpha and dense granules, membrane and cytoskeletal proteins, plat

124 This is consists of dense granules, mitochondria, and specific localised RNA

125 of orthophosphate moieties released from the dense granules of activated platelets, is a procoagulant

126 ogether, these results suggest that the mass-dense granules of D. discoideum are homologous to the ac

127 The mass-dense granules of Dictyostelium discoideum were shown to

128 invasion these components reside within the dense granules of invasive merozoites.

129 finity method detected histamine in electron-dense granules of mast cells in control and injected ski

130 of plants (tonoplast) and the small electron-dense granules of some parasites (acidocalcisomes) where

131 d to the surface of the apical region and to dense granules of sporozoites and merozoites.

132 eriments revealed that TgPSD1 resides in the dense granules of T. gondii and is also found in the par

133 n the biogenesis of melanosomes and platelet dense granules, often referred to as lysosome-related or

134 from exposure to cell extracts that contain dense granule or micronemal proteins.

135 ator of protein transport between post-Golgi dense granule organelles and the Golgi.

136 BD-12 was localized exclusively to the novel dense granules, organelles that also contain precursors

137 rectly from early endosomes, suggesting that dense granules originate from early endosomes in MKs.

138 Platelet dense granules (PDGs) are acidic calcium stores essentia

139 r, Escherichia coli alkaline phosphatase, to dense granules, precluding an in vivo assessment of the

140 predominantly to early endosomes but not to dense granule precursors.

141 mosome X that included the gene encoding the dense granule protein 15 (GRA15).

142 cognize the N-terminal region (aa 41-152) of dense granule protein 6 (GRA6Nt) of the parasite present

143 inases rhoptry proteins 8 and 2 (ROP8/2) and dense granule protein 7 (GRA7).

144 These results show for the first time that a dense granule protein can modulate host signaling pathwa

145 H-2L(d)-restricted T cell epitopes, one from dense granule protein GRA4 and the other from rhoptry pr

146 ain and cytoplasmic tail from the endogenous dense granule protein GRA4 localized to dense granules.

147 Here we show that the dense granule protein GRA7 is phosphorylated but only in

148 ersion of TgPL1 partially colocalized with a dense granule protein in the parasitophorous vacuole spa

149 Within the vacuole, the 28-kDa dense granule protein known as GRA2 is specifically targ

150 tion status of the NTPase, the only parasite dense granule protein that contains disulfide bonds, is

151 lt of the polymorphic protein GRA15, a novel dense granule protein which T. gondii secretes into the

152 port that Toxoplasma secretes GRA24, a novel dense granule protein which traffics from the vacuole to

153 In this report, we identify a novel secreted dense granule protein, GRA14, and show that it is target

154 rophage gene expression, we identify a novel dense granule protein, GRA25.

155 dominant CD8 T cell response to the parasite dense-granule protein GRA6 cannot be generated, leads to

156 y after merozoite invasion and at least some dense granule proteins also use the alternate pathway.

157 in can modulate host signaling pathways, and dense granule proteins can therefore join rhoptry protei

158 MIC2, the cyst matrix protein MAG1, and the dense granule proteins GRA4 and GRA7, were commonly reco

159 We previously demonstrated that secretion of dense granule proteins in permeabilized parasites was au

160 a GDP-bound (Rab6(T25N)) mutant accumulated dense granule proteins in the Golgi and associated trans

161 ic approaches, GTPgammaS enhanced release of dense granule proteins in the permeabilized cell system.

162 By mistargeting of mutant dense granule proteins, we demonstrate that sorting sign

163 extran with both mepacrine and transmembrane dense granule proteins.

164 , however, sporozoites did not exocytose the dense-granule proteins GRA1, GRA2, or GRA4 during PV1 fo

165 Other tachyzoite dense-granule proteins, GRA1, GRA2, GRA4, GRA5, and GRA6

166 An analysis of human platelet dense granules, purified using metrizamide gradient cent

167 SFLLRN- or AYPGKF-induced dense granule release and PKCdelta phosphorylation occur

168 imary and secondary waves of aggregation and dense granule release are strongly induced by nanomolar

169 mechanism of such differential regulation of dense granule release by PKC-delta in platelets.

170 rresponsive CRP and CLEC-2-induced alpha and dense granule release compared with wild-type platelets.

171 These results demonstrate the importance of dense granule release even in the earliest phases of thr

172 a time-dependent manner that coincided with dense granule release in response to protease-activated

173 ombin, collagen, and thromboxane A(2), cause dense granule release independently of thromboxane gener

174 soforms play a differential role in platelet dense granule release mediated by protease-activated rec

175 NSF/SNAP/SNARE/Rab machinery participates in dense granule release using parasite protein components

176 Furthermore, AYPGKF and SFLLRN-induced dense granule release was blocked by rottlerin, a PKCdel

177 However, SFLLRN-induced dense granule release was unaffected in the presence of

178 platelet CD62P expression, alpha-granule and dense granule release, and the classical morphological c

179 hyperresponsiveness to ADP is independent of dense granule release, cyclooxygenase-derived eicosanoid

180 role of individual PKC isoforms in platelet dense granule release.

181 antibodies against proteins from micronemes, dense granules, rhoptries, and plasma membrane showed th

182 Only agonists that caused platelet dense granule secretion activated PKCdelta.

183 hibited the P2Y(12)-mediated potentiation of dense granule secretion and Akt phosphorylation, and did

184 n of 12-LOX or PKC resulted in inhibition of dense granule secretion and attenuation of both aggregat

185 Aggregation, dense granule secretion and calcium mobilisation were si

186 association with a significant reduction in dense granule secretion and impaired aggregation to a pa

187 STX8 therefore specifically contributes to dense granule secretion and represents another member of

188 UNX1 may be common in patients with platelet dense granule secretion defects and mild thrombocytopeni

189 understanding of the disease and the role of dense granule secretion in platelet function.

190 sociations negatively regulate GPVI-mediated dense granule secretion in platelets.

191 haIIbbeta3 activation, and alpha-granule and dense granule secretion in response to the glycoprotein

192 /-) platelets showed a significant defect in dense granule secretion in response to thrombin and CRP.

193 Defective dense granule secretion in RhoG(-/-) platelets limited r

194 on G3 column and able to bind GTP stimulated dense granule secretion in the permeabilized cell secret

195 embryos in which platelet alpha-granule and dense granule secretion is abolished.

196 In contrast, convulxin-induced dense granule secretion was potentiated by rottlerin but

197 In comparison, both aggregation and dense granule secretion were normal following activation

198 ated enhanced agonist-dependent aggregation, dense granule secretion, and fibrinogen binding, compare

199 ith excessive bleeding and impaired platelet dense granule secretion, and highlight transcription fac

200 on triggering adhesion, aggregation, massive dense granule secretion, and thromboxane production.

201 role in protease-activated receptor-mediated dense granule secretion, whereas it functions as a negat

202 PKC-delta positively regulates PAR-mediated dense granule secretion, whereas it negatively regulates

203 duced alpha-granule secretion but stimulated dense granule secretion.

204 d differentially regulates alpha-granule and dense granule secretion.

205 cretion with comparatively modest effects on dense granule secretion.

206 ted peptide, and GPVI/FcRgamma-chain-induced dense granule secretion.

207 ng that they are a consequence of diminished dense granule secretion.

208 receptor signaling, the TxA(2) pathway, and dense granule secretion.

209 hereas it negatively regulates GPVI-mediated dense granule secretion.

210 egree of lysosomes, is secondary to impaired dense granule secretion; and (3) diminished alpha granul

211 measurement of P-selectin) was blocked, and dense-granule secretion (assessed by release of carbon 1

212 activation-dependent increases of alpha and dense-granule secretion and integrin alphaIIbbeta3 activ

213 ence motifs inhibited both alpha-granule and dense-granule secretion in permeabilized human platelets

214 To examine the role of platelet dense-granule secretion in these processes, atherosclero

215 In atherosclerotic mice, reduced platelet dense-granule secretion is associated with marked protec

216 re, we examined the hypothesis that platelet dense-granule secretion modulates thrombosis, inflammati

217 production, adenosine diphosphate (ADP) and dense-granule secretion, and alpha(IIb)beta(3)-mediated

218 l2(-/-) mice, which lack platelet alpha- and dense-granule secretion, show no signs of hemorrhage in

219 trate that cancer cells can promote platelet dense-granule secretion, which is required to augment pl

220 3 gene (HPS3(-/-)) markedly reduces platelet dense-granule secretion.

221 etions are potentiated, whereas PAR-mediated dense granule secretions are inhibited.

222 atelets lacking Lyn or SHIP-1, GPVI-mediated dense granule secretions are potentiated, whereas PAR-me

223 TgLCAT is stored in a subpopulation of dense granule secretory organelles, and following secret

224 n rhoptry proteins, but not in proteins from dense granule secretory organelles; (c) when mutated in

225 or GTP-activated Rab6(Q70L) rerouted soluble dense granule secretory proteins to the Golgi and endopl

226 surrounding the parasite is remodeled by the dense granules, secretory organelles that release an arr

227 me ultrastructure, and in levels of platelet dense granule serotonin, the corresponding phenotypes of

228 t adenosine 5'-diphosphate (ADP) released by dense granules serves as an autocrine signal to potentia

229 ferent organelles--micronemes, rhoptries and dense granules--serves to establish and maintain a paras

230 mbers of dense granules as enumerated by the dense granule-specific fluorescent dye mepacrine.

231 We found no role of MRP4 in ADP dense-granule storage, but MRP4 redistributed cAMP from

232 Human platelet dense granules strongly resemble acidocalcisomes, and we

233 The role of dense granule substances in mediating platelet adhesion

234 anules (delta-SPD) have suggested a role for dense granule substances, in all likelihood adenosine di

235 nd associated with micronemes, rhoptries, or dense granules, the three identified secretory organelle

236 in mice diminishes polyphosphate in platelet dense granules, thereby reducing hemostasis and protecti

237 tein release from secretory vesicles, called dense granules, to maintain the parasite's intracellular

238 All of these characteristics of the platelet dense granules, together with their known acidity and hi

239 adenosine 5'-diphosphate (ADP) secreted from dense granules, trigger platelet activation.

240 through site-specific serotonin release from dense granules, triggering proliferative signaling in he

241 s confirmed by visualization of polyP in the dense granules using 4',6-diamidino-2-phenylindole and b

242 ll as release of adenosine triphosphate from dense granules was also defective in Akt-1-null platelet

243 Release from dense granules was completely ablated and that from alph

244 +)-pyrophosphatase activity of isolated mass-dense granules was stimulated by potassium ions and inhi

245 Dense granules were also shown to contain large amounts

246 tural alterations other than those involving dense granules were detected.

247 sion of serotonin concentrations of platelet dense granules were likewise more severe in double than

248 ive mature protease (p47) is concentrated in dense granules, which are secretory organelles located t

249 Rhoptries, micronemes, and dense granules, which are secretory organelles unique to

250 merous proteins delivered from rhoptries and dense granules, which are secretory organelles unique to

251 urons containing distinctive large, electron-dense granules, which could reliably be used to identify

252 ctron dense deposits at E15 to more electron dense granules with complex patterns of internal structu

253 , known as polyphosphate (polyP), which form dense granules within the cell.