ライフサイエンスコーパス: deoxyhypusine

1 a Lys-50 mutant that blocks modification by deoxyhypusine.

2 pusine or selectively to hypusine but not to deoxyhypusine.

3 educed enzyme intermediate was identified as deoxyhypusine and was shown to occur at a single locus.

4 ural (2S,9R)-hypusine, (2S,9S)-hypusine, and deoxyhypusine- and hypusine-containing peptides are desc

5 imide; KLH conjugates are also made with the deoxyhypusine- and lysine-containing hexapeptides.

6 rain with deletion of YJR070C contained only deoxyhypusine but no hypusine, indicating that YJR070C w

7 hypusine synthase catalyzes the formation of deoxyhypusine by conjugation of the butylamine moiety of

8 Synthetic hypusine and deoxyhypusine can be generated from these reagents.

9 The synthesis of deoxyhypusine catalyzed by this enzyme involves transfer

10 maleimide conjugates, as well as against the deoxyhypusine-containing and lysine-containing hexapepti

11 due in the eIF5A precursor protein to form a deoxyhypusine-containing eIF5A intermediate, eIF5A(Dhp).

12 isplayed a strong preference for binding the deoxyhypusine-containing form of eIF5A, over the eIF5A p

13 were determined in complex with hypusine- or deoxyhypusine-containing peptides.

14 The availability of synthetic hypusine and deoxyhypusine has made it possible to develop analytical

15 The enzyme deoxyhypusine hydroxylase (DOHH) catalyzes the activatio

16 Deoxyhypusine hydroxylase (DOHH) catalyzes the final ste

17 ization of nero, the Drosophila melanogaster deoxyhypusine hydroxylase (DOHH) homologue.

18 Deoxyhypusine hydroxylase (DOHH) is a novel metalloenzym

19 sttranslational modification event requiring deoxyhypusine hydroxylase (DOHH), an enzyme that can be

20 ow that PCBP1 and PCBP2 also deliver iron to deoxyhypusine hydroxylase (DOHH), the dinuclear iron enz

21 involving deoxyhypusine synthase (DHPS) and deoxyhypusine hydroxylase (DOHH).

22 o enzymes, deoxyhypusine synthase (DHPS) and deoxyhypusine hydroxylase (DOHH).

23 teps catalyzed by deoxyhypusine synthase and deoxyhypusine hydroxylase (DOHH).

24 Recombinant human deoxyhypusine hydroxylase (hDOHH) has been reported to h

25 modifying enzymes deoxyhypusine synthase and deoxyhypusine hydroxylase as well as for the cancer-rela

26 s depletion of deoxyhypusine synthase and/or deoxyhypusine hydroxylase causes lethality in adult mice

27 Deoxyhypusine hydroxylase is the key enzyme in the biosy

28 n is catalyzed by deoxyhypusine synthase and deoxyhypusine hydroxylase.

29 on multiple cell processes, suggesting that deoxyhypusine/hypusine biosynthesis could be a promising

30 tic NOD mice that received injections of the deoxyhypusine inhibitor N1-guanyl-1,7-diaminoheptane (GC

31 Deoxyhypusine is a modified lysine residue.

32 novel cofactor called trypanothione and for deoxyhypusine modification of eukaryotic translation ini

33 The hypusine or deoxyhypusine moiety was found to reside in a deep pocke

34 synthetic methods which allow access to (2S)-deoxyhypusine, natural (2S,9R)-hypusine, (2S,9S)-hypusin

35 Deoxyhypusine (Nepsilon-(4-aminobutyl)lysine) is the key

36 The antibodies bind to both hypusine and deoxyhypusine or selectively to hypusine but not to deox

37 thesis, as donor substrates for synthesis of deoxyhypusine (or its analog), and for synthesis of homo

38 e methods involve both the construction of a deoxyhypusine reagent in which the alpha-nitrogen protec

39 -containing eIF5A, indicating a role for the deoxyhypusine residue in binding.

40 s effectively catalyzed hydroxylation of the deoxyhypusine residue in the eIF5A intermediate.

41 at it can carry out the hydroxylation of the deoxyhypusine residue present in the elF5A substrate.

42 lation at the 4-aminobutyl side chain of the deoxyhypusine residue prevents deoxyhypusine synthase-me

43 iolabeled 4-aminobutyl side chain of the [3H]deoxyhypusine residue to 1,3-diaminopropane.

44 In addition to the deoxyhypusine residue, a large portion of the eIF5A poly

45 iolabeled in the 4-aminobutyl portion of its deoxyhypusine residue, was incubated with human deoxyhyp

46 A binding in which the amino group(s) of the deoxyhypusine side chain of the substrate is primarily a

47 s catalyzed by two enzymatic steps involving deoxyhypusine synthase (DHPS) and deoxyhypusine hydroxyl

48 sination, which is catalyzed by two enzymes, deoxyhypusine synthase (DHPS) and deoxyhypusine hydroxyl

49 It was recently shown that the enzyme deoxyhypusine synthase (DHS) promotes early cytokine-ind

50 copies inhibition or knockdown of the enzyme deoxyhypusine synthase (DHS).

51 Purified human deoxyhypusine synthase also exhibited homospermidine syn

52 pusine formation by a selective inhibitor of deoxyhypusine synthase and by its depletion with RNA int

53 two sequential enzymatic steps catalyzed by deoxyhypusine synthase and deoxyhypusine hydroxylase (DO

54 se models for the hypusine-modifying enzymes deoxyhypusine synthase and deoxyhypusine hydroxylase as

55 The reaction is catalyzed by deoxyhypusine synthase and deoxyhypusine hydroxylase.

56 Deoxyhypusine synthase and eIF5A are conserved throughou

57 Here, we tested the hypothesis that deoxyhypusine synthase and, secondarily, hypusinated eIF

58 We discovered that homozygous depletion of deoxyhypusine synthase and/or deoxyhypusine hydroxylase

59 Although both eIF-5A and deoxyhypusine synthase are essential genes for cell surv

60 Inhibition of deoxyhypusine synthase blocked post-transcriptional expr

61 Deoxyhypusine synthase catalyzes the crucial hypusine mo

62 Deoxyhypusine synthase catalyzes the first step in hypus

63 Deoxyhypusine synthase catalyzes the first step in the p

64 Deoxyhypusine synthase catalyzes the first step in the p

65 Deoxyhypusine synthase catalyzes the first step in the t

66 Deoxyhypusine synthase catalyzes the formation of deoxyh

67 In the initial step of this reaction, deoxyhypusine synthase catalyzes the production of NADH

68 The deoxyhypusine synthase competitive inhibitor N(1)-guanyl

69 SS, we identified sequences encoding HSS and deoxyhypusine synthase from various species of the Convo

70 of transcript levels have shown that HSS and deoxyhypusine synthase have also diverged with respect t

71 s suggest a previously unrecognized role for deoxyhypusine synthase in promoting T cell proliferation

72 5A-1 and -2, respectively) as substrates for deoxyhypusine synthase in vitro.

73 Th17, and Treg), but those treated with the deoxyhypusine synthase inhibitor GC7 showed a dose-depen

74 rwinian selection is sufficient to convert a deoxyhypusine synthase into a HSS.

75 Inhibition of deoxyhypusine synthase may provide a strategy for reduci

76 Trypanosoma brucei encodes two deoxyhypusine synthase paralogs, one that is catalytical

77 f previous biochemical investigations of the deoxyhypusine synthase reaction mechanism.

78 ficity of this enzyme and versatility of the deoxyhypusine synthase reaction.

79 tion in the single copy gene, yDHS, encoding deoxyhypusine synthase results in the loss of viability

80 A previous study of human deoxyhypusine synthase revealed four active sites of the

81 of human DOHH along with eIF5A precursor and deoxyhypusine synthase was required for overproduction o

82 endently by duplication of the gene encoding deoxyhypusine synthase, an enzyme involved in the posttr

83 Upon depletion of deoxyhypusine synthase, and consequently of eIF-5A, cess

84 ivity, formation of different complexes with deoxyhypusine synthase, and Km values (1.5 +/- 0.2 vs. 8

85 f eukaryotic initiation factor 5A (eIF5A) by deoxyhypusine synthase, employing spermidine as a butyla

86 xyhypusine residue, was incubated with human deoxyhypusine synthase, NAD, and 1,3-diaminopropane, [3H

87 nsion of this prozyme paradigm to the enzyme deoxyhypusine synthase, which is required for spermidine

88 chain of the deoxyhypusine residue prevents deoxyhypusine synthase-mediated reversal of the modifica

89 e spermidine biosynthetic pathway but retain deoxyhypusine synthase.

90 origin in the duplication of a gene encoding deoxyhypusine synthase.

91 were evaluated as donor substrates for human deoxyhypusine synthase.

92 slation elongation factor eIF5A, mediated by deoxyhypusine synthase.

93 a specific lysine residue (Lys329) in human deoxyhypusine synthase.

94 a) of the conserved lysine residues in human deoxyhypusine synthase.

95 This first structure of a deoxyhypusine synthase.NAD.inhibitor ternary complex und

96 t here at 2.2 A a new Form II crystal of the deoxyhypusine synthase:NAD holoenzyme grown at low ionic

97 enzyme-substrate intermediate formation and deoxyhypusine synthesis activity, indicating that Lys329

98 diate and that it is absolutely required for deoxyhypusine synthesis in the eukaryotic translation in

99 rison of spermidine analogs as inhibitors of deoxyhypusine synthesis, as donor substrates for synthes

100 type enzyme) suggesting that in contrast to deoxyhypusine synthesis, spermidine cleavage can occur w

101 ecently discovered the efficient reversal of deoxyhypusine synthesis.

102 The fact that labeled deoxyhypusine was found after treatment with a reducing

103 reduction of the eIF5A-imine intermediate to deoxyhypusine was reflected by a rapid decrease in the N