ライフサイエンスコーパス: depurinate

1 xic Tyr-123 mutants, although ribosomes were depurinated.

2 ly interfering with the capacity of StxA1 to depurinate 28S rRNA.

3 formed predominantly (> or =99%) the rapidly-depurinating 4-hydroxy estradiol (4-OHE(2))-1-N3Ade addu

4 iol (4-OHE(2))-1-N3Ade adduct and the slower-depurinating 4-OHE(2)-1-N7Gua adduct.

5 proteins (RIPs) are RNA N-glycosidases that depurinate a specific adenine residue in the conserved s

6 Ribosome inactivating proteins (RIPs) depurinate a universally conserved adenine in the alpha-

7 ME(1) was shown to depurinate a variety of partially denatured nucleic acid

8 Upon activation in the cytosol, RTA depurinates a single adenine from position 4324 of rat 2

9 Ricin toxin A-chain (RTA) depurinates a single adenylate on a GAGA stem-loop regio

10 StxA1 catalytically depurinates a well-conserved GAGA tetra-loop of 28S rRNA

11 The cytotoxin ricin disables translation by depurinating a conserved site in eukaryotic rRNA.

12 we discovered a site-specific self-catalyzed depurinating activity in short (14-18 nucleotides) DNA s

13 3,4-Q were bound to DNA in vitro to form the depurinating adduct 4-OHE1(E2)-1(alpha,beta)-N7Gua at 59

14 catechol estrogens (2-OHE2, 4-OHE2), and the depurinating adducts 4-OHE(2)-1(alpha,beta)-N7-guanine (

15 ]P can be activated to metabolites that form depurinating adducts in cells with either high peroxidas

16 ired apurinic sites derived from the loss of depurinating adducts.

17 mediates that covalently bind to DNA to form depurinating adducts.

18 , inhibitor screens, mechanistic analysis of depurinating agents on oligonucleotides and intact ribos

19 ngle-chain ribosomal inhibitory protein that depurinates an adenine residue in the alpha-sarcin loop

20 When rRNA is depurinated and translation is inhibited, the steady sta

21 nter cleft residues significantly impair the depurinating and ribosome inhibitory activity of PAP.

22 ), and (90)FND(92) substantially reduced the depurinating and ribosome inhibitory activity of PAP.

23 A resulted in greater than 3 logs of loss in depurinating and ribosome inhibitory activity.

24 t, all other purine residues in the sequence depurinate at the spontaneous background rate.

25 the loop; the 5'-G residue of the loop self-depurinates at least 10(5)-fold faster than random "spon

26 han the prokaryotic ribosomes, while PAP can depurinate both types of ribosomes.

27 pernatants were analyzed for the presence of depurinating BP-derived adducts by capillary electrophor

28 e, the sarcin-ricin loop (SRL), and were not depurinated by RTA.

29 ntitatively reacts with oxidatively modified depurinated/depyrimidinated (abasic) RNA.

30 P) has been reported to form both stable and depurinating DNA adducts upon activation by cytochrome P

31 e pathways result in formation of stable and depurinating DNA adducts, respectively.

32 elieved to include both stable and unstable (depurinating) DNA adduct formation as well as reactive o

33 Chemically depurinated duplex substrates representing the U5 end of

34 i and that Howardula ribosomal RNA (rRNA) is depurinated during Spiroplasma-mediated protection of D.

35 ed photoacid to effect 5'-detritylation, the depurinating effects of strong acid.

36 racterised by their ability to catalytically depurinate eukaryotic ribosomes, a modification that mak

37 bind ribosomes or depurinate them but could depurinate free RNA.

38 We found that PAP-H depurinates fungal ribosomes in vitro and in vivo, sugge

39 nogenic aromatic hydrocarbons indicates that depurinating hydrocarbon-DNA adducts generate oncogenic

40 Ribosomes were depurinated in yeast cells expressing the precursor form

41 Ribosomes from the P protein mutants were depurinated less than the wild-type ribosomes when treat

42 We present evidence showing that depurinated oligonucleotides react with cytosine-contain

43 The assay uses depurinated oligonucleotides that can form a Schiff base

44 oresis can vary >100-fold if the DNA is acid depurinated prior to Southern blotting.

45 nvestigated to improve the ionization of the depurinated product and assay reproducibility.

46 ns was prepared to test for their ability to depurinate prokaryotic and eukaryotic ribosomes.

47 ovide the first evidence that the ability to depurinate ribosomes and inhibit translation does not al

48 PAPn did not depurinate ribosomes and was non-toxic when expressed in

49 However, it cannot depurinate ribosomes in a translation-independent manner

50 cytotoxicity without altering the ability to depurinate ribosomes in trans and inhibit translation.

51 hese results demonstrate that the ability to depurinate ribosomes in trans in a catalytic manner is r

52 only during their translation and could not depurinate ribosomes in trans in a translation-independe

53 tic activity toward the SRL allows Stx2A1 to depurinate ribosomes, inhibit translation, and exhibit c

54 ion mutants was lost before their ability to depurinate ribosomes.

55 iminated its cytotoxicity and the ability to depurinate ribosomes.

56 Stx2A1 depurinated ribosomes and inhibited translation at a sig

57 Several nontoxic RTA mutants depurinated ribosomes and inhibited translation to the s

58 Stx2A1 depurinated ribosomes at a higher level in vivo and was

59 The RTA mutants that depurinated ribosomes but did not kill cells were not ab

60 The mutant proteins isolated from yeast depurinated ribosomes in vitro, indicating that they wer

61 These mutants depurinated ribosomes only during their translation and

62 I) ribosome-inactivating protein (RIP) that depurinates ribosomes at the alpha-sarcin/ricin loop of

63 ive-site mutant of PAP (PAPx) which does not depurinate rRNA, we present evidence that double-strande

64 Using a PAP variant which depurinates rRNA, inhibits translation but does not dest

65 Appearance of the apurinic site in the self-depurinating stem-loop was confirmed by digestion of pla

66 The detection of a depurinated substrate was enhanced by using a more effic

67 is required for PAP to bind to ribosomes and depurinate the 25 S rRNA, suggesting that it is located

68 hat PAP binds to the ribosomal protein L3 to depurinate the alpha-sarcin/ricin loop and binding of PA

69 We demonstrate that PAP can depurinate the rRNA in trans in a translation-independen

70 mericana, is characterized by its ability to depurinate the sarcin/ricin (S/R) loop of the large rRNA

71 n A chain (RTA), which binds to the stalk to depurinate the sarcin/ricin loop (SRL).

72 2 interactions work together allowing RTA to depurinate the SRL at a much higher rate on the intact r

73 P1 and P2 proteins of the ribosomal stalk to depurinate the SRL in yeast.

74 ; ricin A-chain is an RNA N-glycosidase that depurinates the adenosine at position 4324 and alpha-sar

75 Ricin depurinates the eukaryotic ribosomes more efficiently th

76 ng protein (RIP) that binds to ribosomes and depurinates the highly conserved alpha-sarcin/ricin loop

77 PAP) is a ribosome-inactivating protein that depurinates the highly conserved alpha-sarcin/ricin loop

78 Ricin inhibits protein synthesis by depurinating the alpha-sarcin/ricin loop (SRL).

79 he acceptor site groove prior to binding and depurinating the GAGA tetra-loop.

80 e capped by binding to the cap structure and depurinating the RNAs downstream of the cap.

81 Ricin holotoxin did not bind ribosomes or depurinate them but could depurinate free RNA.

82 nder physiologically relevant conditions and depurinate to release an alkylated nucleobase in a proce

83 ce that PAP is associated with ribosomes and depurinates tobacco ribosomes in vivo by removing more t

84 e yeast, ribosomes from mak8-1 cells are not depurinated when PAP expression is induced in vivo, indi

85 That residue is uniquely depurinated with a rate some 5 orders of magnitude faste

86 purination kinetics demonstrated that Stx2A1 depurinates yeast and mammalian ribosomes and an RNA ste

87 The ribosomes are eventually depurinated, yet cytotoxicity and loss of viability are