ライフサイエンスコーパス: derivatization

1 of other lipids during liquid-phase analyte derivatization.

2 he number of (13)C atoms incorporated during derivatization.

3 alogy to the classic procedure of heavy-atom derivatization.

4 to distinguish PCs and PSs without chemical derivatization.

5 not have an easy "handle" for bioreversible derivatization.

6 ion in comparison with phenylhydrazine (PHN) derivatization.

7 e of increasing the effectiveness of in situ derivatization.

8 no unmodified PS species identified prior to derivatization.

9 cal activity, or intermediates for synthetic derivatization.

10 e lupeol at 610nm after post chromatographic derivatization.

11 MR studies, molecular modeling, and chemical derivatization.

12 ps, thus providing the potential for further derivatization.

13 ucts which are highly predisposed to further derivatization.

14 cylindole building blocks suited for further derivatization.

15 luable C7-boroindoles that enable further C7-derivatization.

16 velopment of stabilized fluorophores through derivatization.

17 taining N-glycans without the need of sample derivatization.

18 and the last one is very mild conditions of derivatization.

19 d no necessity of sample preconcentration or derivatization.

20 n impact ionization, with no need for sample derivatization.

21 e compared with HPLC coupled with pre-column derivatization.

22 uires a combination of methods and/or sample derivatization.

23 unds an intriguing class of dyes for further derivatization.

24 ples), including examples of late-stage drug derivatization.

25 strated equivalent results with conventional derivatization.

26 ese critical intermediates without synthetic derivatization.

27 tive for many alkene classes and facilitated derivatization.

28 HP) in human urine is possible without prior derivatization.

29 in high yields and selectivities for further derivatization.

30 -tandem mass spectrometry (LC-MS/MS) without derivatization.

31 uantum mechanical calculations, and chemical derivatizations.

32 On-tissue analyte derivatization addresses these issues by selectively tag

33 ated electrospray deposition of liquid-phase derivatization agents as a means of on-tissue analyte de

34 Two derivatization agents were compared, TFAI and N,O-bis(tr

35 ut derivatization, compared to methods using derivatization agents.

36 and 5-position of the pyrrole, while further derivatization allows for the introduction of substituen

37 The direct on-probe derivatization allows high sample throughput with a typi

38 This derivatization altogether abolishes fluorescein fluoresc

39 Using p-toluenesulfonyl isocyanate for derivatization and a stable isotope labeled internal sta

40 rum GC-MS metabolomics in fruits using a new derivatization and a two dimensional peak deconvolution

41 ts and an amino acid measurement system with derivatization and electrophoretic separation integrated

42 t by using 2,4-dinitrophenylhydrazine (DNPH) derivatization and electrospray ionization-tandem mass s

43 ine by ion-pair HPLC method with post-column derivatization and fluorimetric detection is proposed.

44 hich include pseudouridine-specific chemical derivatization and gas phase dissociation of RNA during

45 As a proof of principle, we applied MCF derivatization and GC-APCI-TOFMS to the detection of cha

46 ree independent approaches consisting of the derivatization and halogenation of the CH3Hg standard so

47 13)C- and (15)N-metabolic labeling, multiple derivatization and ionization types, and heuristic filte

48 re was used in parallel with mercaptoethanol derivatization and LC-MS(2) analysis to demonstrate the

49 acid epimerization were analyzed by chemical derivatization and LC-MS/MS.

50 tion reagent and parameters affecting in the derivatization and microextraction steps were studied an

51 Under optimum conditions of derivatization and microextraction steps, the method yie

52 s, this method requires no sample cleanup or derivatization and presents improved values for the LOD

53 uided approach is more efficient than random derivatization and screening.

54 The compositions of the derivatization and separation buffers were optimized to

55 the development and evaluation of a chemical derivatization and tandem mass spectrometry method that

56 lysis, supported by chemical degradation and derivatization, and biosynthetic considerations.

57 ly derivatized metabolites and chemicals for derivatization, and in a complete cell incubated medium.

58 This derivatization approach overcomes the need for costly la

59 The optimum conditions of derivatization are: 500muL of isopropanolic eluate obtai

60 silylation techniques as well as in further derivatizations are also described.

61 provides numerous opportunities for further derivatization as well as site-selective functionalizati

62 Comparison of the alternate isotope-coded derivatization assay (AIDA) using deuterium versus (15)N

63 ded the first analogs fit for human use, and derivatization at C12 led to 12-epi-pleuromutilins with

64 The rapid and robust diacetyl derivatization at low temperatures (e.g., -20 degrees C,

65 Derivatization at the 9-position relies on facile reacti

66 Derivatizations avail cyclohexenes bearing four contiguo

67 alkyne-labeled 1c can be employed for toxin derivatization by click chemistry with an azide-containi

68 ore developed a linkage-specific sialic acid derivatization by dimethylamidation and subsequent amida

69 Direct access to 2-enoic acids allows derivatization by numerous approaches.

70 ivatization of TDGO by TFAI in comparison to derivatization by silylating agents.

71 resonance (HSQC-NMR) or, following suitable derivatization, by gas chromatography-mass spectroscopy

72 orward biomolecular attachment and multistep derivatization capabilities to these nanoparticles and,

73 Here, we demonstrate novel derivatization chemistry for depolymerization/desulfatio

74 Optimized conditions for sample derivatization, chromatographic separation, and MS/MS de

75 ificity was achieved by measuring E2 without derivatization, compared to methods using derivatization

76 The derivatization conditions can potentially lead to oligos

77 TFAI derivatization conditions were optimized and the method

78 utilizing mass spectrometry with or without derivatization, cost-effective and high throughput metho

79 aracterized using thermal desorption in situ derivatization coupled to a GCxGC-ToF/MS system.

80 yzed via a procedure based on injection-port derivatization coupled to gas chromatography-tandem mass

81 w tracer method based on gas-phase stripping-derivatization coupled to liquid chromatography-mass spe

82 Chemical derivatization coupled to X-ray photoelectron spectrosco

83 alkynes, SNO-OCTs contain multiple sites for derivatization, display stability under a variety of com

84 a more versatile building block for further derivatizations, e.g., the cyanide 14.

85 , and the reagent selection was based on the derivatization effectiveness and the capability of MS si

86 Critically, diacetyl derivatization enables the differentiation of discrete m

87 require many steps including extraction and derivatization ending in GC/MS or HPLC analysis, which m

88 The derivatization enhanced the ionisation efficiency of vit

89 Derivatization, extraction and rinsing steps are not req

90 A simultaneous derivatization/extraction method followed by liquid chro

91 involves deproteination of milk followed by derivatization/extraction of the HAAs in the supernatant

92 abolites in human plasma, based on precolumn derivatization followed by HPLC-inductively coupled plas

93 ol could be released from these lignins upon derivatization followed by reductive cleavage, a degrada

94 ion methods: acidolysis; thioacidolysis; and derivatization followed by reductive cleavage; were appl

95 products and pharmaceuticals, allowing rapid derivatization for investigation of structure-activity r

96 mers and their transformation products using derivatization gas chromatography-mass spectrometry.

97 In this work, we refined a derivatization gas chromatography-mass spectroscopy tech

98 The amount of NaBPr4 added for derivatization has been found to be the main factor resp

99 Real-time chemical derivatization has been incorporated for analyzing anabo

100 e been developed and numerous post-synthesis derivatizations have allowed for their exploitation in b

101 phic methods, HPLC-HPAEC-PAD and post-column derivatization HPLC-UV-Vis, were compared for carbohydra

102 A chemical isotope labeling or isotope coded derivatization (ICD) metabolomics platform uses a chemic

103 with phosphate buffer and sodium cyanide for derivatization in alkaline conditions.

104 d using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN)

105 12) with no sample carryover and with inline derivatization in the case of poorly ionizing compounds.

106 or modifications by late-stage semisynthetic derivatization in ways that enhance their biological pot

107 The validated method includes derivatization, in which FAs are sent through esterifica

108 urrent status of microbial BIA synthesis and derivatization, including rapid developments in the past

109 These results indicate FMOC derivatization is a suitable alternative for fumonisins

110 The derivatization is carried out on a 50-100 mg mineral sam

111 Derivatization is often required via chemical reaction.

112 to liquid chromatography/mass spectrometry (derivatization-LC/MS, d-LC/MS).

113 Subsequent derivatization led to a fragment collection featuring re

114 C10 amide of lead compound 2 and subsequent derivatization led to novel carbon- and nitrogen-linked

115 Derivatization makes small, poorly ionizable or retained

116 The derivatization method enables one to identify various ca

117 , we present a highly repeatable sialic acid derivatization method to allow subclass-specific MALDI-T

118 (ICD) metabolomics platform uses a chemical derivatization method to introduce a mass tag to all of

119 resent an in-gel protein N-terminal chemical derivatization method using (N-succinimidyloxycarbonylme

120 come this problem, we developed an on-tissue derivatization method with Girard's reagent T (GirT) in

121 A simple derivatization methodology is shown to extend the applic

122 However, previously developed on-fiber derivatization methods have been hindered by poor loadin

123 Solid phase microextraction (SPME) on-fiber derivatization methods have facilitated the achievement

124 We used sialic acid linkage-specific derivatization methods to improve separation and the lev

125 In addition, we explored compatible sample derivatization methods.

126 that cannot be determined accurately by DNPH derivatization methods.

127 zation techniques and stable isotope labeled derivatization methods.

128 es a versatile method for the site-selective derivatization of a broad range of vinyl arene substrate

129 oordinated palladium catalyst and allows the derivatization of a diverse range of heterocycles, inclu

130 atalytic step is employed for the asymmetric derivatization of alpha,beta-unsaturated aldehydes to 4,

131 We demonstrate here that derivatization of amine metabolites with tandem mass tag

132 Derivatization of biologically active peptides by conjug

133 This research showed that in situ derivatization of cannabinoids through addition of an N-

134 strategies for the synthesis and late-stage derivatization of complex molecules, but the chemical sp

135 The broad substrate scope, including the derivatization of complex natural products and neutral a

136 em high-resolution mass spectrometry reveals derivatization of CstR by both inorganic tetrasulfide an

137 Here, we report that both incomplete derivatization of Cys side chains and overalkylation of

138 Derivatization of Cys78 with maleimide creates a solutio

139 mophthalic anhydride (TBPA) was used for the derivatization of free NH2-groups.

140 te, was used as a convenient reagent for the derivatization of histamine, tyramine, tryptamine and 2-

141 on the surface of HSA molecules due to prior derivatization of HSA molecules with appropriate functio

142 PAAC) enables high resolution 3-D photoclick derivatization of hydrogels and tissues.

143 eview provides a comprehensive survey of the derivatization of hydrogen-terminated, oxide-free silico

144 Derivatization of malvidin-3-glucoside using stearoyl ch

145 ributed elements, which is crucial following derivatization of metabolites.

146 high-throughput LC-MS(n) workflow for rapid derivatization of peptide mixtures.

147 Fast online chemical derivatization of peptides with an aromatic label for en

148 ce a more flexible methodology for selective derivatization of phenolic moieties in complex biomolecu

149 r the synthesis of chlorins has entailed the derivatization of porphyrins.

150 gies for preparation of the ring and further derivatization of preformed oxetane-containing building

151 eve the pyrolyis reaction and facilitate the derivatization of pyrolysis products, by enabling the ma

152 ntageous over classical methods as clean up/ derivatization of samples is not required for formaldehy

153 emoenzymatic approach for immobilization and derivatization of sialic acids.

154 The derivatization of silicon surfaces can have profound eff

155 In summary, derivatization of specific amino acid residues in conjun

156 ure was elucidated through the semisynthetic derivatization of starting material via chemoselective a

157 Moreover, by-products are not formed during derivatization of TDGO by TFAI in comparison to derivati

158 of pyrroles into 3-methyl-pyrrolines and the derivatization of the 3-methyl-substituent arising from

159 this method is further demonstrated through derivatization of the chiral aziridine products to obtai

160 re or site-directed mutagenesis and chemical derivatization of the claudin-2 pore.

161 thine ylide, and dipolarophile), and further derivatization of the cycloadduct are reported.

162 ve also been discovered, commonly leading to derivatization of the internal carbon atom.

163 These new routes allow for the derivatization of the methoxyphenyl and chlorophenyl rin

164 lass presently inaccessible by semisynthetic derivatization of the natural product, by its late-stage

165 Following derivatization of the pair of spiked, isotopic reference

166 The derivatization of the phosphonic acid functionality of F

167 Finally, derivatization of the pi-electron framework of [n]cumule

168 Derivatization of the products by tert-butyl deprotectio

169 n the preparation of mixed bis-triazoles and derivatization of the protein BSA with fluorescent dye a

170 hloric acid hydrolysis, followed by chemical derivatization of the released monomer sugars and quanti

171 blished via the nitrocatechol ring, allowing derivatization of the side chain for future lead optimiz

172 Reduction of the nitro group followed by derivatization of the so formed anilines 14 gave the N-a

173 Thus, a derivatization of the steroids prior to the measurement

174 Derivatization of the target amines required minimal sam

175 Chemical derivatization of these diterpenoids has demonstrated th

176 Derivatization of these phenyl rings can lead to two ver

177 tantrone-containing RNA ligands based on the derivatization of this intercalator with two neamine moi

178 Rational systematic derivatization of this scaffold to maximize Eis inhibiti

179 trolled choices of highly diastereoselective derivatizations of 1.

180 Mechanochemical derivatizations of N- or C-protected amino acids were pe

181 oducts by subjecting our products to several derivatizations of the Meldrum's acid products as well a

182 hydroboration and subsequent stereospecific derivatizations of the secondary boronates.

183 of core histones that were separated without derivatization on a homemade capillary column packed wit

184 This method combines (13)C1-acetyl derivatization on the protein level, proteolysis by low-

185 both neutral and sialylated glycans without derivatization or labeling.

186 Such differences in SA derivatization, particularly O-acetylation, could have c

187 Prior to derivatization, plant ITCs were isolated and purified us

188 tudies were carried out using five different derivatizations possessing two NO2, five fluoro, two flu

189 id phase extraction chromatography, and FMOC-derivatization preceded analysis by reverse phase HPLC w

190 zinc in acidic solution, hydrophobization by derivatization, preconcentration on C18 cartridge, and L

191 es with guanidine avoiding its NH-protection/derivatization prerequisite for electronic modulation.

192 ed a cross-validation of the newly developed derivatization procedure and quantification based on mon

193 We present a derivatization procedure to chemically stabilize these c

194 nd HMFu in baby formula samples, without any derivatization process.

195 ation, proportionality, and stoichiometry in derivatization processes were determined by gas chromato

196 e enzymatic products during the trapping and derivatization processes.

197 A practically simple derivatization protocol for determining the enantiopurit

198 was explored for the development of a novel derivatization protocol, and the products were subjected

199 tion, sample clean-up, preconcentration, and derivatization protocols.

200 otein capture, N-glycan release, fluorescent derivatization, purification, and preconcentration steps

201 two orders of magnitude if TFAI is used for derivatization rather than silyl derivatizing agents (e.

202 In addition, reproducibility of the derivatization reaction was observed over 1 week (RSD <

203 by application to a common steroid-labeling derivatization reaction.

204 was found to be the suitable solvent for the derivatization reaction.

205 Three derivatization reactions have been carried out with N-me

206 is rationale has established that the use of derivatization reactions widens the scope of targeted mo

207 iophenes participate in a variety of in situ derivatization reactions, showcasing that these borylate

208 The main advantage of the new derivatization reagent 1,2-dimethylimidazole-5-sulfonyl

209 Xanthydrol was used as a derivatization reagent and parameters affecting in the d

210 MS) method is described that employs a novel derivatization reagent for the measurement of serum estr

211 reproducibility and standard lifetime due to derivatization reagent reactivity.

212 or a reactive, toxic, and otherwise unstable derivatization reagent standard.

213 methodology, combined with the use of a mild derivatization reagent, allowed communol pyrolysates fro

214 E fibers can now be reproducibly loaded with derivatization reagent, from an easy to use and safe pla

215 oquinolyl-N-hydroxysuccinimidyl carbamate as derivatization reagent.

216 geted application scenarios of the suggested derivatization route are enabled in the frame of a singl

217 rmination in conjunction with a novel sample derivatization scheme in which glucose oxidase and lacta

218 yme immobilization procedure, and the online derivatization scheme, the available sampling frequency

219 d chain length at the newly accessible G4-OH derivatization site.

220 y mass spectrometry with no prior separation/derivatization step has been developed.

221 The method uses a derivatization step that can be conducted either online

222 nol in finfish using an off-line dansylation derivatization step to enhance signal intensity.

223 The concept involves a derivatization step with a bifunctional reagent containi

224 For this purpose, a derivatization step with the new reagent 3,4-diaminopyri

225 ofile in mussel samples, avoiding a previous derivatization step, required when gas chromatographic a

226 The method requires no derivatization steps or sample extraction procedures tha

227 tion ionization (MALDI), typically requiring derivatization steps to overcome their low ionization ef

228 d does not require hydrolysis, extraction or derivatization steps, but rather a simple "dilute and sh

229 This application broadens the scope for derivatization strategies on tissue surfaces to elucidat

230 merization and post-polymerization chain-end derivatization strategies.

231 xy Gdm, and thereby provides a new synthetic derivatization strategy and implicates a potential uniqu

232 We have implemented a derivatization strategy based on carbamylation which con

233 three extracts by GC/MS using the silylation derivatization technique revealed 53 compounds, 17 of th

234 ompounds are susceptible to multiple further derivatizations through Suzuki coupling or nitrile trans

235 terms of optimal injection port temperature, derivatization time and sample: N-methyl-N-(trimethylsil

236 ucleosides and nucleotides generally require derivatization to induce gelation, guanosine and its cor

237 and positive ion modes, as well as chemical derivatization to methyl ester derivatives and analysis

238 migration, a facile low temperature diacetyl derivatization to stabilize regiospecificity, and tandem

239 Natural water experiments, including derivatization to transform primary and secondary amines

240 acetate and water, with preconcentration and derivatization, to produce stable fluorescent derivative

241 The derivatization treatment leads to characteristic band sh

242 6%, 346.6% as measured by o-phthaldialdehyde derivatization, trichloroacetic acid precipitation and h

243 l format after O-benzylhydroxylamine (O-BHA) derivatization under aqueous conditions.

244 MS studies show that analog 4 undergoes easy derivatization under mild conditions by reaction with OH

245 ation agents as a means of on-tissue analyte derivatization using 2-picolylamine; we were able to det

246 (MSI) is combined with an on-tissue chemical derivatization using diethyldithiocarbamate (DDTC).

247 mpling in conjunction with (i) online sample derivatization using glucose oxidase to generate H2O2, w

248 lcohols at room temperature with no chemical derivatization using Mg(II)bisporphyrin as a host.

249 ochloric acid (HCl), followed by amino acids derivatization using N,O-bis(trimethylsilyl)trifluoroace

250 and G1 was enhanced by post-column chemical derivatization using pyridinium bromide perbromide.

251 e mosquito body was conducted without sample derivatization using selected reaction monitoring of mas

252 thylsilyl) trifluoroacetamide (BSTFA), where derivatization using TFAI occurred quicker and under mil

253 ectrometry with in situ hexamethyldisilazane derivatization, using a thermal separation probe to perf

254 ecule chemical analysis and metabolomics for derivatization, vaporization, chromatography, and ioniza

255 a GC-MS injector to simulate on line in situ derivatization-volatilization-transfer steps.

256 Manual and in situ derivatization was assayed and the chromatographic respo

257 Liquid-liquid extraction followed by derivatization was employed for the identification of th

258 Furthermore, in-port derivatization was optimized in terms of optimal injecti

259 Sites selected for Alexa 488 (C5) derivatization were in the alpha-helical domain (residue

260 nd high quality MS(2) data resulted from GRT derivatization were observed as key benefits of this app

261 mine L-SOP levels in CSF employing precolumn derivatization with (5-N-succinimidoxy-5-oxopentyl)triph

262 hy-tandem mass spectrometry (GC-MS/MS) after derivatization with 1-(trifluoroacetyl)imidazole (TFAI)

263 (CE-LIF) method that analyzes Ado and Ino by derivatization with 2,4,6-trinitrobenzenesulfonic acid t

264 y been detected spectrophotometrically after derivatization with 2,4-dinitrophenylhydrazine (DNPH) to

265 nal and its fatty acid metabolites following derivatization with 2-diphenylacetyl-1,3-indandione-1-hy

266 re improved through ion/ion-mediated peptide derivatization with 4-formyl-1,3-benezenedisulfonic acid

267 ds in human plasma and serum using precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidy

268 Pre-column derivatization with 9-fluorenylmethyl chloroformate (FMO

269 was established, after total hydrolysis, by derivatization with a new chiral reagent, (5-fluoro-2,4-

270 Reactions involving hydroxyl group derivatization with a variety of electrophilic reagents

271 The specific detection via derivatization with an ethanolic potassium hydroxide sol

272 aldehydic (ALDO)-OxPLs was enabled by prior derivatization with bifunctional carbonyl-reactive reage

273 ) is presented, and its subsequent selective derivatization with Bronsted acids, MeOTf, Ph2ECl (E = P

274 :25% ammonium hydroxide (70:30:4:2 v/v/v/v), derivatization with Carr-Price reagent and video densito

275 or tandem mass spectrometry after pre-column derivatization with chiral reagent revealed spontaneous

276 A new and sensitive pre-column derivatization with dabsyl chloride followed by dispersi

277 Derivatization with dabsyl chloride leads to improving s

278 tone using a tissue homogenizer, followed by derivatization with dansyl chloride and analysis by liqu

279 of AA and BA was developed, using selective derivatization with dansyl chloride followed by HPLC wit

280 After pre-column derivatization with dansyl-chloride a Kinetex C18 core-s

281 nd serotonin) were determined by LC-UV after derivatization with dansyl-chloride in both ground coffe

282 of biological samples after facile one-step derivatization with dimethylglycine based on the princip

283 ranslational modifications, as well as rapid derivatization with diverse fluorophores for imaging.

284 % ammonium hydroxide (75:15:10:1.6 v/v/v/v), derivatization with Dragendorff reagent and scanning den

285 Here, derivatization with Girard T reagent was used to charge-

286 ntibodies with a new analytical approach via derivatization with Girard's Reagent T (GRT) and subsequ

287 ng direct acetonitrile extraction, precolumn derivatization with hydroxylamine, and LC-MS/MS analysis

288 13)C-isotope labeling of polyphenols through derivatization with isotopic dansyl chloride reagents an

289 and alpha2,6-linked sialic acids by covalent derivatization with methylamine.

290 ocyanates (ITCs) determination that involves derivatization with N-acetyl-l-cysteine (NAC) and separa

291 multimethod for human blood plasma based on derivatization with o-phenylenediamine under acidic cond

292 roperties of ametantrone by single or double derivatization with other RNA recognition motifs, which

293 rene, elution with acetonitrile and off-line derivatization with p-dimethylaminocinnamaldehyde in ace

294 tegy is based on rapid autosampler-in-needle-derivatization with p-toluenesulfonylhydrazine (TSH).

295 cation-exchange solid-phase extraction, (2) derivatization with pentafluorophenacyl trifluoromethane

296 permethylation or by specific carboxyl group derivatization with the possibility of discrimination be

297 ent tryptic digestion and N-terminal peptide derivatization with the ultraviolet chromophore AMCA yie

298 ysis of certain classes of metabolites is by derivatization with various types of tags to increase io

299 pathways may be created by means of cavity "derivatization" with selected guest molecules resulting

300 need for pre-functionalization or substrate derivatization, would therefore be valuable.