ライフサイエンスコーパス: desalting

1 thin materials for molecular separations or desalting.

2 o those from conventional guanidination with desalting.

3 asured directly from reaction mixtures after desalting.

4 the membrane to small ions allows efficient desalting.

5 MS analysis of the digestion mixture without desalting.

6 Rapid desalting and a low volume connection to an electrospray

7 ed using the described protein precipitation/desalting and cation-exchange solid-phase extraction iso

8 ents: (1) isolation by protein precipitation/desalting and cation-exchange solid-phase extraction, (2

9 s were identical regardless of the method of desalting and concentrating of protein samples, samples

10 e for in-well trypsin digestion, followed by desalting and concentrating the digestion products in th

11 ries of sample handling processes, including desalting and concentrating, to be performed directly on

12 ause of the post-electrophoresis extraction, desalting and concentration steps.

13 Desalting and concentration takes approximately 5 min wh

14 onable analytical procedures with respect to desalting and enrichment were established.

15 To ensure an efficient electrospray process, desalting and exchanging the biomolecular solutions into

16 All digestates were then combined for desalting and MALDI-TOF/TOF mass spectrometric analysis.

17 Desalting and sample concentration follows each fraction

18 applied to tryptic peptide mixtures prior to desalting and spotting onto MALDI-TOF plates.

19 eps involved in a sample preparation such as desalting and various chromatographic purification schem

20 ctions is the time-consuming chromatography, desalting, and concentration steps required to prepare a

21 n steps, including depletion, fractionation, desalting, and concentration.

22 e concentration, buffer exchange, digestion, desalting, and matrix/analyte cocrystallization for MALD

23 buffer exchange, protease digestion, peptide desalting, and, in the case of MALDI-MS, matrix and anal

24 general, and highly efficient, rapid in-line desalting approach using a small gel cartridge to assist

25 rotein A affinity chromatography followed by desalting by gel filtration and was characterized by ami

26 Following purification and desalting by reversed-phase HPLC, buffer exchange with M

27 yzed carbohydrates by replacing ion-exchange desalting cartridges with evaporative removal of HCl und

28 ddition, it provides an effective method for desalting cellular RNA samples having complex matrixes,

29 e antibody-drug conjugate (ADC) using native desalting conditions, we maintain the intact bivalent st

30 isobaric tag labeled glycopeptides after C18 desalting could be readily enriched by SAX and RAX cartr

31 ium dodecyl sulfate (SDS) and following with desalting/delipidation of the sample by chloroform/metha

32 We have developed a microfabricated desalting device that meets both requirements, thus prov

33 Two-stage desalting (during solid phase extraction and on the anal

34 Desalting efficiency and analyte loss was evaluated with

35 roximately 80% and demonstrate the exquisite desalting efficiency with high-performance electrospray

36 This approach allows effective sample desalting, enrichment, sequential elution, and MS detect

37 Desalting is not needed prior to MALDI-TOF MS.

38 edures are detrimental to MALDI-MS, and thus desalting is required before the digestion products can

39 This desalting method directly separates highly polar, ionic

40 MALDI-TOF, applying our successive on-plate desalting method that eliminates the insensitivity of th

41 sample preparation is improved using a novel desalting method that utilizes the hydrophobic surface o

42 ca columns; however, these entail subsequent desalting methodologies and consequent sample losses and

43 versed-phase liquid chromatography (IP-RPLC) desalting methods we previously employed do not effectiv

44 Its removal by various solid-phase desalting methods, catalase treatment, or freeze drying

45 We evaluated a range of desalting methods, including spin columns, dialysis memb

46 IMAC procedure was significantly improved by desalting methylated peptides, followed by gradient elut

47 Specifically for desalting NaCl, this enhancement of unipolar cation cond

48 octadecyl amine (ODA) to be employed in the desalting of complex mixtures and the results are compar

49 This protocol involves the desalting of nucleic acids using ammonium acetate precip

50 Desalting of protein solutions is demonstrated for ESI-M

51 Elimination of the need for desalting of samples after reaction raised the possibili

52 The complete desalting of samples, which is necessary for IEF, tends

53 Size-selective separation and desalting of small model molecules ( approximately 200-1

54 graphic media for the microconcentration and desalting of SUPREX samples.

55 phase purification approach also facilitates desalting of the captured oligonucleotides, which is ess

56 ut the need for intermediate chromatography, desalting, or concentration steps.

57 e presence of excess MnCl2 followed by rapid desalting over a gel filtration column resulted in the r

58 the molecular ion region of the analytes, a desalting procedure of the MBM sample directly on the MA

59 reby eliminating conventional time-consuming desalting procedures required for downstream analysis of

60 anes; the first one with MWCO below 5700 for desalting protein samples, and the second one with a hig

61 attractive alternative to other methods for desalting proteins prior to mass spectrometry analysis.

62 This tip-based purification/desalting protocol has two distinct advantages over prev

63 the stringent high-resolution and extensive desalting requirements that are essential to the pinpoin

64 applied to samples all at once as opposed to desalting samples one-by-one for 5 min each.

65 assay, which included PCA extraction, online desalting, separation of the high-energy phosphates on a

66 (ammonium sulfate precipitation followed by desalting size exclusion chromatography) to get purified

67 A second desalting step, achieved by dialysis utilizing a membran

68 y using the second-dimension separation as a desalting step.

69 ometric analysis was feasible after a single desalting step.

70 The N-glycan modification, digestion, and desalting steps were performed using a single-pot method

71 ant RapiGest (RG), eliminates alkylation and desalting steps, and accomplishes the reduced tryptic di

72 ALDI experiment, without having to resort to desalting steps.

73 substitute for conventional cation-exchange desalting techniques.

74 ocessor for integrated DNA sequencing sample desalting, template removal, preconcentration, and CE an

75 Carbamylation could be minimized by desalting the cyanylation reaction before cleavage or by

76 separation can be identified by trapping and desalting the fractions onto a series of reversed phase

77 d a porous graphitic carbon (PGC) column for desalting the peptides found in the unretained fraction.

78 hort reversed-phase chromatographic step for desalting the sample, rapid DMS separation of the analyt

79 With on-chip desalting, the limit of quantitation for histidine spike

80 Following trypsin digestion and desalting, the mitochondrial samples were analyzed by na

81 cially available ZipTip C-18 and Aspire RP30 Desalting Tip.

82 However, the former requires desalting to be MS-compatible, and the latter requires f

83 on-exchange (SCX) followed by reversed-phase desalting to remove Ficoll, a synthetic polymer, for HX-

84 The system also features fast sample loading/desalting using a vented column approach to improve samp

85 Efficient desalting was demonstrated for both DNA and protein samp

86 On-column enrichment and desalting was demonstrated for large sample volumes (>40

87 Even after desalting, we show that the choice of matrix still plays

88 enhance mass spectral quality often require desalting, which presents as a bottleneck in matrix-assi

89 rapid digestion, peptide concentration, and desalting while maintaining slow H/D exchange conditions

90 orward workflow without fraction pooling and desalting while showing comparable performance to the ot