ライフサイエンスコーパス: destain

1 Brilliant Blue R-250 for 16 to 24 h and then destained.

2 ed using capacitance measurements and FM-dye destaining.

3 ed fainter staining and significantly faster destaining.

4 stimulation at 0.5 Hz evoked about 66 +/- 5% destaining.

5 ostriatal kinetics with depression of FM1-43 destaining.

6 differences in the rate or extent of FM1-43 destaining.

7 tly smaller effect on stimulus-evoked FM1-43 destaining.

8 fold more rapidly than restoration of FM2-10 destaining.

9 ally released into the bathing medium during destaining.

10 ital dye FM1-43 in isolated NMJ preparations destained after application of BzATP (30 microm).

11 Partially bleached regions could be destained, although the rate of destaining was lower tha

12 alcium ionophore calcimycin stimulate FM1-43 destaining and quantal release in csp mutants at 32 degr

13 und a fixed proportionality between staining/destaining and summed endplate potentials (EPPs) represe

14 In addition, the kinetics of FM1-43 destaining and synaptic depression measured in the prese

15 BDNF was absent during dye loading, imaging, destaining and whole-cell recordings, these results demo

16 for their size range, stained with ethidium, destained, and a quantitative electronic image obtained.

17 g the required separation, staining, virtual destaining, and detection steps.

18 ue is relatively slow and requires staining, destaining, and scanning before the data can be processe

19 iminating the need for fixing, staining, and destaining as required for the conventional procedures.

20 f csp mutant neuromuscular junctions fail to destain at 32 degrees C after K+ depolarization, and tha

21 Moreover, quantitative analysis of FM1-43 destaining at these orphan release sites reveals similar

22 xin (type A, C, or D) blocked exocytosis and destaining, but intense nerve stimulation still did not

23 Stained terminals can subsequently be destained by repeating nerve stimulation in the absence

24 ain solution can, therefore, be recycled for destaining CBB-stained gels.

25 In destained cells, amylopectin essentially represents the

26 The temperature dependence of FM1-43 destaining correlated well with the effect of temperatur

27 itional sample preparation, electroblotting, destaining, etc.

28 These populations of destaining events are distinct in both brightness and ki

29 tion model, indicates that the small, slowly destaining events may be mediated by a narrow approximat

30 nerve stimulation in the absence of dye; the destaining evidently reflects escape of dye into the bat

31 Gel destaining following Coomassie Brilliant Blue (CBB) stai

32 n the evoked and spontaneous rates of FM1-43 destaining from terminals in CA1 stratum radiatum, mostl

33 FM1-43, and these restained regions could be destained fully by nerve stimulation.

34 Finding neurotransmission in the absence of destaining implied that rapidly endocytosed RRP vesicles

35 additional dye-loaded vesicles showed rapid destaining in response to strong stimulation and were al

36 Destaining in the FM2-10 loaded boutons during 3 min of

37 The kinetics of FM1-43 destaining indicate that synapses from a single neuron h

38 This BzATP evoked destaining is blocked by oxidized ATP (100 microm) and B

39 nerve terminals, but not activity-dependent destaining, is reduced.

40 Finally, we have shown that vesicle-destaining kinetics are not strongly influenced by the s

41 aded with kinetics substantially slower than destaining of FM dye, indicating that full-collapse fusi

42 -89; 10 microm), then washed for 60 min, the destaining of FM1-43 fluorescence evoked by the same sti

43 Observations of the dynamic staining and destaining of FM1-43 in frog motor nerve terminals sugge

44 However, destaining of FM1-43-labeled vesicles is abolished by lo

45 s to observe activity-dependent staining and destaining of functional synapses.

46 Activity-dependent staining and destaining of the endocytic probe FM1-43 were directly c

47 here was no direct dependence of staining or destaining on stimulus frequency, as would be expected i

48 unning buffer; in this case a brief one-step destaining procedure follows electrophoresis.

49 which converts the independent staining and destaining procedure into a single step.

50 In contrast, SDS-CGE requires no staining or destaining procedures and the peak quantitation is super

51 Using different dye staining and destaining protocols we were able to resolve two effects

52 orders of magnitude faster than the overall destaining rate (timescale of seconds) of these dyes fro

53 TrkB-IgG prevented the enhancement of FM1-43 destaining rate caused by induction of long-term potenti

54 lation of the RRP, BDNF increased the evoked destaining rate of FM4-64 only during the initial phase

55 gel slices, the procedure combines washing, destaining, reduction and alkylation into a single step.

56 Staining and destaining require only 30 min, and the method is compat

57 to 60 min following electrophoresis, with no destaining required.

58 Comparison with FM dye destaining revealed that kiss-and-run strongly prevailed

59 ing of postsynaptic responses and styryl dye destaining showed that after an initial round of exocyto

60 Nonradioactive destain solution can, therefore, be recycled for destain

61 a hexacyanoferrate-thiosulphate redox-based destain solution.

62 ally friendly manner and allows recycling of destaining solution.

63 st (hours) and does not require staining and destaining steps.

64 ining offers the advantage of no staining or destaining steps.

65 (10 Hz/2 min) elicited a frequency-dependent destaining that peaked at 20% reduction in fluorescence.

66 Cooling also reduced the terminal FM1-43 destaining that was induced by direct depolarization wit

67 r staining with Coomassie brilliant blue and destaining, the gels were analyzed with a video area den

68 reserpine-treated mice, quinpirole decreased destaining to a greater extent, and at a lower dose, con

69 tain removal with Kimwipes helps in reducing destain use and in reducing organic liquid waste, and it

70 ons could be destained, although the rate of destaining was lower than normal.

71 ations loaded with the optical probe FM1-43, destaining was reduced by latrunculin treatment, suggest