ライフサイエンスコーパス: destaining

1 ed using capacitance measurements and FM-dye destaining.

2 stimulation at 0.5 Hz evoked about 66 +/- 5% destaining.

3 ostriatal kinetics with depression of FM1-43 destaining.

4 differences in the rate or extent of FM1-43 destaining.

5 tly smaller effect on stimulus-evoked FM1-43 destaining.

6 fold more rapidly than restoration of FM2-10 destaining.

7 ally released into the bathing medium during destaining.

8 ed fainter staining and significantly faster destaining.

9 alcium ionophore calcimycin stimulate FM1-43 destaining and quantal release in csp mutants at 32 degr

10 und a fixed proportionality between staining/destaining and summed endplate potentials (EPPs) represe

11 In addition, the kinetics of FM1-43 destaining and synaptic depression measured in the prese

12 BDNF was absent during dye loading, imaging, destaining and whole-cell recordings, these results demo

13 g the required separation, staining, virtual destaining, and detection steps.

14 ue is relatively slow and requires staining, destaining, and scanning before the data can be processe

15 iminating the need for fixing, staining, and destaining as required for the conventional procedures.

16 Moreover, quantitative analysis of FM1-43 destaining at these orphan release sites reveals similar

17 xin (type A, C, or D) blocked exocytosis and destaining, but intense nerve stimulation still did not

18 ain solution can, therefore, be recycled for destaining CBB-stained gels.

19 The temperature dependence of FM1-43 destaining correlated well with the effect of temperatur

20 itional sample preparation, electroblotting, destaining, etc.

21 These populations of destaining events are distinct in both brightness and ki

22 tion model, indicates that the small, slowly destaining events may be mediated by a narrow approximat

23 nerve stimulation in the absence of dye; the destaining evidently reflects escape of dye into the bat

24 Gel destaining following Coomassie Brilliant Blue (CBB) stai

25 n the evoked and spontaneous rates of FM1-43 destaining from terminals in CA1 stratum radiatum, mostl

26 Finding neurotransmission in the absence of destaining implied that rapidly endocytosed RRP vesicles

27 additional dye-loaded vesicles showed rapid destaining in response to strong stimulation and were al

28 Destaining in the FM2-10 loaded boutons during 3 min of

29 The kinetics of FM1-43 destaining indicate that synapses from a single neuron h

30 This BzATP evoked destaining is blocked by oxidized ATP (100 microm) and B

31 nerve terminals, but not activity-dependent destaining, is reduced.

32 Finally, we have shown that vesicle-destaining kinetics are not strongly influenced by the s

33 aded with kinetics substantially slower than destaining of FM dye, indicating that full-collapse fusi

34 -89; 10 microm), then washed for 60 min, the destaining of FM1-43 fluorescence evoked by the same sti

35 Observations of the dynamic staining and destaining of FM1-43 in frog motor nerve terminals sugge

36 However, destaining of FM1-43-labeled vesicles is abolished by lo

37 s to observe activity-dependent staining and destaining of functional synapses.

38 Activity-dependent staining and destaining of the endocytic probe FM1-43 were directly c

39 here was no direct dependence of staining or destaining on stimulus frequency, as would be expected i

40 unning buffer; in this case a brief one-step destaining procedure follows electrophoresis.

41 which converts the independent staining and destaining procedure into a single step.

42 In contrast, SDS-CGE requires no staining or destaining procedures and the peak quantitation is super

43 Using different dye staining and destaining protocols we were able to resolve two effects

44 orders of magnitude faster than the overall destaining rate (timescale of seconds) of these dyes fro

45 TrkB-IgG prevented the enhancement of FM1-43 destaining rate caused by induction of long-term potenti

46 lation of the RRP, BDNF increased the evoked destaining rate of FM4-64 only during the initial phase

47 gel slices, the procedure combines washing, destaining, reduction and alkylation into a single step.

48 Staining and destaining require only 30 min, and the method is compat

49 to 60 min following electrophoresis, with no destaining required.

50 Comparison with FM dye destaining revealed that kiss-and-run strongly prevailed

51 ing of postsynaptic responses and styryl dye destaining showed that after an initial round of exocyto

52 ally friendly manner and allows recycling of destaining solution.

53 ining offers the advantage of no staining or destaining steps.

54 st (hours) and does not require staining and destaining steps.

55 (10 Hz/2 min) elicited a frequency-dependent destaining that peaked at 20% reduction in fluorescence.

56 Cooling also reduced the terminal FM1-43 destaining that was induced by direct depolarization wit

57 r staining with Coomassie brilliant blue and destaining, the gels were analyzed with a video area den

58 reserpine-treated mice, quinpirole decreased destaining to a greater extent, and at a lower dose, con

59 ons could be destained, although the rate of destaining was lower than normal.

60 ations loaded with the optical probe FM1-43, destaining was reduced by latrunculin treatment, suggest