ライフサイエンスコーパス: detergent

1 in retains high affinity ProTx-II binding in detergent.

2 ore complexes upon contact with membranes or detergent.

3 gle complexes remain very similar to that in detergent.

4 rown in both the absence and the presence of detergent.

5 ing on protein concentration and presence of detergent.

6 the transporter and MBP in nanodiscs and in detergent.

7 such regulation does not require maltose in detergent.

8 ation can be controlled by timed exposure to detergent.

9 hobic protein to be stored in the absence of detergent.

10 r-physiologic environment without the use of detergents.

11 cles without the requirement of conventional detergents.

12 ation of the enzyme from membranes with mild detergents.

13 e hydrocarbon binding site occupied by these detergents.

14 OWCs) such as pharmaceuticals, hormones, and detergents.

15 from their native membrane environment using detergents.

16 bile salts, a class of physiological anionic detergents.

17 s their extraction from native sources using detergents, a step that can lead, possibly irreversibly,

18 y proceeds in the absence of dialysis and/or detergent absorbents, and A2AR assimilation into synthet

19 osphate replacement in automatic dishwashing detergents (ADW).

20 nt contrasts, together with an estimation of detergent aggregation number around the protein, permits

21 cameric pore complexes after the addition of detergent and are hemolytically inactive.

22 ces, or after washing the glass surface with detergent and bare hands, was also observed, with measur

23 by resistance to solubilization in nonionic detergent and by copatching with a raft-resident sphingo

24 that this reaction requires the presence of detergent and does not take place in liposomes but in mi

25 ted a reduced tendency to solubilize in mild detergent and exit the endoplasmic reticulum.

26 ne-quarter-density caveolin-1 was soluble in detergent and formed a continuous population with the ca

27 ale measured as lower solubility in nonionic detergent and in the microscopic scale evident as the pr

28 rsed lower-density caveolin-1 was soluble in detergent and increased after the application of tension

29 tern of mobilities and spectral features, in detergent and liposomes, for residues at the pore domain

30 density, resistance to being solubilized by detergent and quenching of fluorophores within the vesic

31 sensitivity of the DeltapstA1 mutant to both detergent and reactive oxygen species.

32 he hospital WDS with a chlorinated, alkaline detergent and subsequent superchlorination followed by m

33 ures of M2, many of which were determined in detergent and/or with shorter constructs that are not fu

34 ously implementing legislation limiting P in detergents and increasing wastewater reuse across the en

35 Detergents and membrane mimetics can solubilize membrane

36 validated the method with a wide variety of detergents and membrane proteins.

37 l for stabilizing the AMPAR-CNIH3 complex in detergents and overlap with the contacts made between Gl

38 When detergents and phospholipid membranes are dispersed in a

39 oaches, whose limitations include the use of detergents and the micelle-mediated association of prote

40 tracellular virions are treated with various detergents and/or salts.

41 s with the original ATAC-seq protocol (using detergent) and treating them with CRISPR.

42 , a replacement for environmentally damaging detergents, and muconate, a renewable precursor to polye

43 S100) that comigrate with endogenous lipids, detergents, and/or micelles during blue native gel elect

44 Among all the variables tested, the use of detergent appeared to affect the total mass of fibers re

45 A critical flaw with detergent approaches is the removal of the protein from

46 Detergents are used most often for solubilizing membrane

47 Detergents are usually used to extract these bio-macromo

48 ing municipal WRRFs is mostly from soaps and detergents as dissolved organic matter, its fate can be

49 either a conformational change or binding of detergent at the binding site in a detergent micelle env

50 The presence of detergents at increasing micelle concentrations, on the

51 For satisfactorily solubilizing detergents, at concentrations much greater than the crit

52 o be 12.9% +/- 0.7% of that for conventional detergent-based lysis in yielding detectable protein.

53 rotein crystallization, and to visualize the detergent belt for Cryo-EM studies.

54 retinal from Rho in an in vitro phospholipid/detergent bicelle system.

55 ed rhodopsin reconstituted into phospholipid/detergent bicelles with rhodopsin reconstituted into det

56 membrane proteins studies require the use of detergents, but because of the lack of a general, accura

57 tability, and photodynamics were analyzed in detergent by CD, stationary, as well as time-resolved op

58 h analyses of both proteins solubilized with detergents (C12E8 and octyl-PoE) and supported by the fo

59 hilic interaction chromatography to mitigate detergent carryover and improve liquid chromatography-ma

60 Detergent chemistry, dominated by oxidizing agents, was

61 Low temperature expression and detergent choice significantly affected binding and turn

62 ibe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be us

63 In the absence of detergent, ClyA slowly forms soluble oligomers.

64 The available detergent-compatible liquid crystals are negatively char

65 Current methods for inspecting protein-detergent complex (PDC) interfaces require high concentr

66 al mass of fibers released the most, yet the detergent composition (liquid or powder) or overdosing o

67 lysis, the cleavage rate strongly depends on detergent concentration, because the reaction proceeds o

68 e fluorescence anisotropy was independent of detergent concentration.

69 In contrast, at detergent concentrations comparable with or below the CM

70 , whereas prefibrillar species required high-detergent conditions to retrieve, consistent with membra

71 Detergents containing oxidizing agents assisted with Ag

72 on fabrics may be negated when treated with detergents containing strong oxidants, such as chlorine.

73 show how to reliably and easily estimate the detergent corona diameter and select the smallest size,

74 d dimer of yeast iso-1-cytochrome c with the detergents, CYMAL-5, CYMAL-6, and omega-undecylenyl-beta

75 hydrophobic lipid environment minimizing the detergent dependence often seen in assays with membrane

76 rate loading into supported biomembranes was detergent-dependent, as evidenced by even colocalization

77 To gain insights into the mechanism of detergent destabilization of GPCRs, we used atomistic mo

78 suggest a model whereby bile salts or other detergents destabilize ToxR, increasing its interaction

79 position (liquid or powder) or overdosing of detergent did not significantly influence microplastic r

80 The addition of detergents did not influence the extractability of light

81 isolated transporter in a dodecyl-maltoside detergent environment.

82 fact, toxicity tests indicated that residual detergent exhibited greater adverse response than the re

83 eoliposomes, reconstituted from a microsomal detergent extract, lost their activity when made with an

84 We also exchanged native troponin detergent extracted fibers with reconstituted troponin c

85 graphy, the myofilament-Ca(2)(+) response of detergent-extracted fiber bundles, and used proteomic ap

86 s associated with C. trachomatis isolated by detergent extraction, but it may represent contamination

87 Our approach avoids detergent extraction, purification, and reconstitution u

88 unoprecipitation-Western blotting using high-detergent extracts revealed a variety of SDS-stable low-

89 Low-detergent extracts tested by 82E1 enzyme-linked immunoso

90 ct that membrane proteins retain activity in detergent extracts) that phospholipid environment is a s

91 er (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP) and ether extr

92 t digestibility of crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), crude

93 6) using DMI, and dietary digestible neutral detergent fiber and fatty acid contents as predictor var

94 tips into Alconox, a commercially-available detergent, followed by rinsing, we were able to reuse pi

95 ver enzyme-substrate complex and to optimise detergents for membrane protein study.

96 ed by critical selection of elution buffers, detergents for protein solubilization, and stabilizers t

97 nt proteins are very hydrophobic and require detergents for purification, which presents major obstac

98 The detergent form (i.e., powder vs liquid) was the other de

99 rradiation and/or washing in seven different detergent formulations was followed by NP characterizati

100 terminations but limits enzyme efficiency in detergent formulations.

101 vel peptide-based lipid nanodiscs, which are detergent-free and possesses size flexibility, and their

102 Here we describe a rapid cell-free and detergent-free co-translation method for producing full-

103 ree expression technologies, even completely detergent-free membrane protein characterization protoco

104 t amphipathic or hydrophobic substrates in a detergent-free native or artificial membrane environment

105 ools in biomedical research that can offer a detergent-free solubilization of membrane proteins maint

106 ns from their native environment in a single detergent-free step.

107 The detergent-free synthesis of membrane protein/nanodisc sa

108 Here, detergent-free washing experiments were conducted and re

109 rodomains, although our approach is valuably detergent-free.

110 ments using CRISPR technology and removal of detergent from the cell lysis buffer.

111 py, kinetically resolves the dissociation of detergents from membrane proteins and protein unfolding.

112 Deoxycholic acid, a strong detergent, greatly enhanced the conjugation yield likely

113 he headgroup and alkyl chain correlates with detergent harshness and suggests new avenues to develop

114 ever virus (HFV)-infected patients with 0.1% detergents has been recommended for virus inactivation a

115 ex in peptide-based nanodiscs, containing no detergents, has been demonstrated, which are characteriz

116 all systems studied, despite differences in detergent headgroup charge or dipole orientation.

117 The removal of detergent, however, resulted in increased background and

118 ysosomal permeability through a lysomotropic detergent in cells devoid of Bax/Bak1 restores autophagi

119 w how to obtain information of the amount of detergent in complex with a membrane protein, essential

120 We tested the influence of detergent in cultures of BCG and M. tuberculosis strains

121 The presence of detergent in growth media and a capsule on BCG were asso

122 Addition of detergent in milk can cause food poisoning and other com

123 usly attributed to ESAT-6 is due to residual detergent in the preparations.

124 that allows quantification of pure or mixed detergents in complex with membrane proteins.

125 Bile salts act as steroidal detergents in the gut, and could also interact with pept

126 ores via the same pathway in the presence of detergent, in which an unstructured, monomeric intermedi

127 lic organisms exhibit poor stability in mild detergents, indicating that instability is inherent to t

128 e found to have increased phosphorylated and detergent-insoluble alpha-synuclein deposits.

129 aspase-8 is proteolytically processed within detergent-insoluble ASC-enriched protein complexes prior

130 Notably, detergent-insoluble ferritin accumulates in RPE cells an

131 and hemin caused aggregation of PrP(C) to a detergent-insoluble form, limiting iron uptake.

132 ion, but it may represent contamination with detergent-insoluble host lipids rather than being an int

133 f plasma membrane (PM) and the corresponding detergent-insoluble membrane (DIM) fraction were analyze

134 uingly, this lipid profile is reminiscent of detergent-insoluble membrane microdomains, although our

135 e show that CATylation mediates formation of detergent-insoluble NC aggregates.

136 of reactive oxygen species were comparable, detergent-insoluble protein aggregates containing phosph

137 of multiple RPs, exceptional accumulation of detergent-insoluble proteins including multiple RPs, and

138 ined the 3-dimensional structure of knobs in detergent-insoluble skeletons of P falciparum 3D7 schizo

139 transgenic worms, reduced phosphorylated and detergent-insoluble tau accumulation, and reduced tau-me

140 penetrates the brain, reduces the levels of detergent-insoluble tau, neuronal loss and reverses neur

141 ylcholine and sphingomyelin; Irganox 1010 (a detergent); insulin; and rhodamine B-and show that usefu

142 ure medium showed that virus inactivation by detergents is annulled at physiological serum concentrat

143 standing how membrane proteins interact with detergents is of fundamental and practical significance

144 chondrial complex I using the branched-chain detergent lauryl maltose neopentyl glycol.

145 nd similar to denaturation by the nonnatural detergent lauryldimethylamine-N-oxide (LDAO).

146 , it has been hypothesized that emulsifiers, detergent-like molecules that are a ubiquitous component

147 Since the detergent-like property of BAs causes liver damage at hi

148 Because of detergent-like toxicity, BA levels must be tightly regul

149 These observations suggest a "detergent-like" mechanism, where lipids are extracted fr

150 rption frequencies of common constituents of detergent (linear alkyl benzene sulphonate).

151 nonspecific contacts between the protein and detergent/lipid micelles in the electrospray droplet.

152 g of drugs to membrane proteins from that of detergents, lipids and cofactors is challenging.

153 The choice of detergents, lipids, and ligands during purification dete

154 for detection and quantification of anionic detergent (lissapol) in milk.

155 of industrial products, from biomedicines to detergents, lubricants, and coatings.

156 We show that the chemical properties of the detergents mediate the charge state, both during ionizat

157 Remarkably, under the optimized detergent micelle conditions, Abeta40 and Abeta42 showed

158 ctivity of purified human or mouse P-gp in a detergent micelle environment.

159 inding of detergent at the binding site in a detergent micelle environment.

160 iers indirectly by increasing the associated detergent micelle size, but cardiolipin stabilizes by di

161 Importantly, we demonstrate that in the detergent micelle system, commonly used for the enzymati

162 teractions between BamA, B, D and E, and the detergent micelle that suggest communication between BAM

163 tions within the receptor and stiffening the detergent micelle.

164 ty increases with the size of the associated detergent micelle.

165 e used to describe, respectively, folding in detergent micelles and folding within a bilayer, which e

166 ated rhomboid proteases, can be used both in detergent micelles and in liposomes, and contain red-shi

167 protection of membrane proteins compared to detergent micelles and less shielding to those protein r

168 Interestingly, not only detergent micelles but also lipid bilayer nanodiscs or b

169 c peptide inhibitor QZ59-SSS was observed in detergent micelles compared with native or artificial me

170 utants embedded in either a lipid bilayer or detergent micelles of alkylmaltosides and alkylglucoside

171 reased stability, they are often superior to detergent micelles or liposomes for membrane protein sol

172 racellular loop 3 (ICL3) was crystallized in detergent micelles using vapor-phase diffusion.

173 Accordingly, various types of detergent micelles were extensively screened to identify

174 exhibit significantly increased stability in detergent micelles whilst preferentially occupying a sin

175 iberate an intact V-type ATPase complex from detergent micelles, a result that cannot be achieved by

176 ical properties of P-gp in native membranes, detergent micelles, and when reconstituted in artificial

177 ironments such as membranes, lipid vesicles, detergent micelles, bicelles, oriented bilayers, or nano

178 ion of LHCII has been extensively studied in detergent micelles, but recent results have indicated th

179 ins have been performed with the proteins in detergent micelles, locked in specific conformations and

180 ane domain, reconstituted in liposomes or in detergent micelles, revealed in all cases the existence

181 e helicity of this region in the presence of detergent micelles, which was prevented by an AH-disrupt

182 ia contacts between proteins and mixed lipid/detergent micelles.

183 imilar photocycle intermediates as GtACR1 in detergent micelles.

184 embrane environment than are the widely used detergent micelles.

185 mutations that accelerate GlpG's folding in detergent micelles.

186 d at physiological pH in the membrane and in detergent micelles.

187 ndent of maltose for purified transporter in detergent micelles.

188 e scale of a protein folding into and out of detergent micelles.

189 t bicelles with rhodopsin reconstituted into detergent micelles.

190 nts are highly similar in lipid vesicles and detergent micelles.

191 helical packing, and the interpenetration of detergent molecules between transmembrane alpha-helices.

192 f GPCRs by octylglucoside: (i) highly mobile detergent molecules form small micelles around the recep

193 Instead, the presence of bound detergent molecules inside the barrel suggests that Pput

194 transmembrane helices allowed penetration of detergent molecules into the core of the receptor.

195 oncerning the different components (protein, detergent molecules) of detergent-solubilized membrane p

196 oluble, inactive oligomers in the absence of detergent much faster than the reduced monomer, providin

197 n Mistic in the presence of the zwitterionic detergent n-dodecylphosphocholine (DPC).

198 for RDC measurements are compatible with the detergents needed in membrane protein studies.

199 hat places the hydrocarbon moieties of these detergents next to the heme.

200 remained similar to the crystal structure in detergent on the timescale of our simulations.

201 e also tested the impact of a large panel of detergents on PSII stability and found that very few are

202 ity assay for the enzyme either dissolved in detergent or embedded in GUV membranes.

203 ly charged bilayers, with no requirement for detergent or fusion-promoting proteins, and deliver larg

204 lonal antibodies showed that the addition of detergent or reducing agent improved extraction efficien

205 the environment, and they may be unstable in detergents or fail to crystallize.

206 integral membrane proteins are visualized in detergents or other artificial systems, an important lay

207 embranes, and LHCII solubilized in different detergents or trapped in polymer gel.

208 roximately 100 nm, the rate-limiting step in detergent- or membrane- induced pore assembly is the uni

209 rete clustering of samples based on level of detergent (p0.05) in milk.

210 leic acid copolymer was used to effect a non-detergent partial solubilization of thylakoids from spin

211 describe the interactions with cholesterol, detergents, peptides, and integral membrane proteins and

212 are mainly used in surfactants, lubricants, detergents, pharmaceuticals and cosmetics while medium c

213 om extracts of these organs were enriched by detergent phase separation, lectin affinity chromatograp

214 itored the changes in hydrodynamic radius of detergent/phosphatidylcholine particles during the micel

215 the specimens were perfused using a combined detergent/polar solvent decellularization protocol.

216 its 3.6-4.0 A from the nearest carbon of the detergents, positioned to act as a relay in radical abst

217 E-S complexes by temperature, compounds, or detergent promotes release of amyloidogenic Abeta.

218 Traditionally achieved with detergents, purification procedures can be costly and ti

219 ort assays by measuring substrate binding to detergent-purified SbMATE protein.

220 the charge state, both during ionization and detergent removal.

221 r Pmt4 activity in vitro We demonstrate that detergent requirements and acceptor substrates of yeast

222 scopy revealed that betaIII spectrin forms a detergent-resistant cytoskeletal network at these sites.

223 also showed the enrichment of sterols in the detergent-resistant membrane (DRM) fractions obtained fr

224 Although CFTR has been detected in a detergent-resistant membrane fraction prepared from airw

225 We demonstrate that detergent-resistant membrane nanodomains, also known as

226 subunitsBandCand preserving the integrity of detergent-resistant membrane organization.

227 ation between NS2 and E2 localization to the detergent-resistant membranes (DRM) and HCV particle ass

228 says to demonstrate that NS2 associates with detergent-resistant membranes (DRM) in a p7-dependent ma

229 oylation levels correlated with targeting to detergent-resistant membranes (rafts) and to caveolin-1.

230 ion proteins are associated with sterol-rich detergent-resistant membranes in yeast and plant cells.

231 PMA, but not DiC8, targeted PKCalpha to detergent-resistant membranes, and disruption of these d

232 HtrA from cellular lysates partitioned into detergent-resistant membranes, which contain cholesterol

233 -encoded vIRF-1 targets to the mitochondrial detergent-resistant microdomains via direct interaction

234 l cellular prion protein (PrP(C)) resides in detergent-resistant outer membrane lipid rafts in which

235 Detergent-resistant, ubiquitinated and hyperphosphorylat

236 mg fibers/g textile washed, without and with detergent, respectively), the overall microplastic fiber

237 and artificially adulterated milk (0.2-2.0% detergent) samples revealed clear differences in wavenum

238 ed by repetitive topical applications of the detergent SDS or by high-dose UV B radiation, IR/IGF-1R(

239 The association of LDAP3 with LDs was detergent sensitive, but the protein bound with similar

240 swatches in deionized water with or without detergent showed a range of silver release.

241 ady-state and dynamic contractile indices in detergent-skinned guinea pig (Cavia porcellus) cardiac m

242 stratified these proteins according to their detergent solubility profiles.

243 fferent antenna sizes was achieved with mild detergent solubilization of photosynthetic membranes and

244 using protein crosslinking followed by ionic detergent solubilization, we show that Hsp93 directly bi

245 ane protein that was efficiently isolated by detergent solubilization.

246 ions revealed that the native structures of detergent solubilized MPs were not always retained in th

247 like MP conformations in the gas phase using detergent solubilized proteins is often challenging and

248 We show that limited proteolysis of the detergent-solubilized and purified yeast flippase may re

249 The detergent-solubilized complex adopts a three-bladed prop

250 re, we report on the preparation of a stable detergent-solubilized complex between Rho* and a heterot

251 Stability of detergent-solubilized G-protein-coupled receptors (GPCRs

252 Analysis of detergent-solubilized Hrd1.KI cells indicates that the c

253 ture of these hydrophilic protein domains in detergent-solubilized LHCII.

254 components (protein, detergent molecules) of detergent-solubilized membrane protein complexes.

255 studied the kinetics of ATP hydrolysis using detergent-solubilized MetNI.

256 125)I-labeled radioligand to the unpurified, detergent-solubilized MP.

257 As with lipid nanodiscs, reconstitution of detergent-solubilized MsbA into the polymer nanodiscs si

258 LRET-based measurements of detergent-solubilized PglB from C. lari allowed direct c

259 egment (ROS) membranes shifted the resulting detergent-solubilized protein migration through a gel fi

260 All previous studies on human SQR have used detergent-solubilized protein.

261 Crystal structures of detergent-solubilized rat TRPV6 in the closed state have

262 need to be isolated and reconstituted from a detergent-solubilized state into a well-defined and cont

263 primarily been conducted with protein in the detergent-solubilized state rather than embedded in a me

264 ibition of Rho kinases in neurons diminished detergent-soluble and -insoluble tau through a combinati

265 P301S-tg mice, associated with a decrease in detergent-soluble tau species.

266 ily achieved by washing with a dilute acidic detergent solution at 4 degrees C.

267 lity of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the

268 tein fluorescence quenching, bound Fe(2+) in detergent solution with low micromolar affinity.

269 h RhoGC protein expressed in HEK293 cells in detergent solution.

270 These technical advances include novel detergents, specialised crystallography techniques as we

271 tants had similar sensitivity to osmotic and detergent stress and lipopolysaccharide profile and an i

272 hibited increased sensitivity to osmotic and detergent stress, lacked very long lipopolysaccharide, w

273 the C-terminus is essential for function and detergents strongly affect structure and activity, the m

274 lucoside are more denaturing than long chain detergents such as dodecylmaltoside.

275 state, and it is well-known that short chain detergents such as octylglucoside are more denaturing th

276 Earlier studies of HAfp in detergent support (1) N-helix/turn/C-helix structure at

277 We prepared kidney membrane analogs of detergent-susceptible membranes, depleted of cholesterol

278 ric structure and washing conditions (use of detergents, temperature, wash duration, and sequential w

279 embrane proteins solubilized in conventional detergents tend to undergo structural degradation, neces

280 Bile acids function not only as detergents that facilitate lipid absorption but also as

281 f liquid and powdered commercially available detergents that span a wide range of different chemistri

282 Specifically, in detergent the GluA2-A793F formed an unstable complex wit

283 investigation in DPC, implying that in this detergent, the protein structure is distorted.

284 Here we used calixarene detergent to solubilize and purify wild-type non-aggrega

285 leting its hydrophobic C-terminus and adding detergents to enhance solubility.

286 The detergent-treated Bcl-xL forms a dimer through three-dim

287 OV or herpes simplex virus type 1 (HSV-1) in detergents-treated cell culture medium containing variou

288 However, 0.1% detergent treatment did not inactivate EBOV in blood sam

289 atory test results were not affected by 0.1% detergent treatment of blood samples, in contrast with 1

290 Using low-concentration detergent treatments, the holo complex is dissected into

291 The different structures of HAfp in detergent vs membrane could be due to the differences in

292 efficient of determination for prediction of detergent was 0.94 for calibration and 0.93 for validati

293 After extraction with non-denaturing detergents, we affinity-purified 785 endogenously tagged

294 it similar stability profiles across various detergents, where stability increases with the size of t

295 sis strains grow bacteria in the presence of detergent, which also strips the mycobacterial capsule.

296 roteins was abolished with the addition of a detergent, which shifts the factors to the non-translati

297 propensity of dodecylphosphocholine (DPC), a detergent widely utilized in NMR studies of membrane pro

298 d human alpha4beta3delta GABAARs purified in detergent with [(3)H]azietomidate and a barbiturate, [(3

299 m and subsequently assembled with SNAP-25 in detergent with the correct 1:1 stoichiometry.

300 Reaction of the purified protein, in detergent, with the thiol-reactive N-ethylmalemide (NEM)