ライフサイエンスコーパス: deuterium isotope effect

1 and is consistent with a significant primary deuterium isotope effect.

2 dehydrogenation reaction exhibited the same deuterium isotope effect.

3 dependence, and a larger than usual upfield deuterium isotope effect.

4 inding specificity was assessed by using the deuterium isotope effect.

5 alysis and inhibition, we determined solvent deuterium isotope effects.

6 postulated on the basis of spectroscopy and deuterium isotope effects.

7 sidered unlikely given the lack of a solvent deuterium isotope effect above the breakpoint in the pH

8 Ru and zero order in phosphine, and kinetic deuterium isotope effects all point to a mechanism invol

9 Kinetic deuterium isotope effects along with K(m) values permitt

10 The non-competitive intramolecular deuterium isotope effect, an estimate of the intrinsic i

11 r, the observation in N694C of a significant deuterium isotope effect, anaerobic reduction of iron by

12 .0064 (assuming 5.7 as the intrinsic primary deuterium isotope effect and 1.054 as the product of the

13 Results on kinetic deuterium isotope effect and quenching studies are in co

14 ental steps were associated with an hydrogen/deuterium isotope effect and that glycolate alpha-deprot

15 An analysis of noncompetitive deuterium isotope effects and competitive tritium isotop

16 Solvent deuterium isotope effects and maps of the electrostatic

17 Furthermore, comparative deuterium isotope effects and the relative rates of inac

18 values of the theoretically relevant kinetic deuterium-isotope effect and its dependence on temperatu

19 decreases in activity, the measured solvent deuterium isotope effects, and changes in the pH depende

20 ith the NADPH-supported P450 reactions, high deuterium isotope effects ( approximately 7) were seen i

21 while a small pH-independent primary kinetic deuterium isotope effect (approximately 1.3) is observed

22 Because of a large deuterium isotope effect, approximately 7, the quantum a

23 For Mg(2+)-assisted reactions, the solvent deuterium isotope effects are 1.23 and 0.25 for general

24 Values of substrate and solvent deuterium isotope effects are consistent with the kineti

25 Furthermore, we demonstrate that solvent deuterium isotope effects are involved in the thermal de

26 The enzymatic deuterium isotope effects are lower by a factor of 2, bu

27 V/Km deuterium isotope effects are observed for both substrat

28 Significant deuterium isotope effects are obtained in these reaction

29 cal shifts, a Steiner-Limbach correlation, a deuterium isotope effect as well as quantitative values

30 Apparent intrinsic deuterium isotope effects as high as 15 were measured.

31 onsistent with a stepwise mechanism with the deuterium isotope effect at C3 being only on the decarbo

32 The observed 'inverse' deuterium isotope effect at pH < 8 can be explained by a

33 Secondary deuterium isotope effects at C-3 were 2.5% at pH 7 and 3

34 Deuterium isotope effects at C2 of aspartate and heavy a

35 With NADP, deuterium isotope effects at C3 of 1.17 and 1.08 for di-

36 ean lipoxygenase 1 have indicated very large deuterium isotope effects, but have not been able to dis

37 According to a pronounced deuterium isotope effect (CH3OD), this motion of heavy a

38 reduced values with deuterium substitution (deuterium isotope effect) characteristic of MAO B.

39 This relatively high intramolecular deuterium isotope effect confirmed the initial hydrogen

40 The deuterium isotope effects (D)(V/K) for (4R)-[4-(2)H]-NAD

41 The deuterium isotope effects (D)V and (D)(V/K) for (4R)-[4-

42 kylation of DMN had a high intrinsic kinetic deuterium isotope effect ((D)k(app) approximately 10), w

43 method yielded an apparent intrinsic kinetic deuterium isotope effect ((D)k) of 15.

44 tions of the intrinsic primary and secondary deuterium isotope effects ((D)k = 2.7, (alpha)(-D)k = 1.

45 Primary deuterium isotope effect data were measured for the wild

46 Solvent pH and deuterium isotope-effect data are also used to evaluate

47 Deuterium isotope effects demonstrate that solvent proto

48 The intrinsic primary deuterium isotope effect determined from single-waveleng

49 A trend of decreasing kinetic deuterium isotope effect for E225I > wild-type > mutant

50 The pH dependence and deuterium isotope effect for reduction of isolated compo

51 A large kinetic deuterium isotope effect for the amine activation proces

52 The deuterium isotope effect for the reaction was determined

53 to solvent accessibility by detection of the deuterium isotope effect for Y(Z) oxidation and by 2H ES

54 ly fast in the catalytic cycle; high kinetic deuterium isotope effects for all four lauric acid hydro

55 In contrast, significant suppression of the deuterium isotope effects for CYP2B1 occurred only with

56 (ii) Solvent deuterium isotope effects for hydrolysis of Z-Gln-Gly by

57 Inter- and intramolecular kinetic deuterium isotope effects for phenacetin O-deethylation

58 Intramolecular deuterium isotope effects for the benzylic hydroxylation

59 )/k(D) = 9.7 and 6.8, respectively), whereas deuterium isotope effects for the naphthyl and biphenyl

60 type of KR is found to exhibit a significant deuterium isotope effect (for 9 vs d(1)-9).

61 r oxidation of malate, while the equilibrium deuterium isotope effect from deuteration at C-2 of the

62 Noncompetitive measurements of the primary deuterium isotope effect give a value of ca. 40 which is

63 , high noncompetitive intermolecular kinetic deuterium isotope effects (>/= 5.5) were observed for al

64 In addition, a solvent deuterium isotope effect has been taken as evidence agai

65 2-(2)H4]choline ((18)F-D4-FCH), based on the deuterium isotope effect, has been developed.

66 Kinetic parameters and primary deuterium isotope effects have been determined for wild-

67 er conditions of catalytic turnover, kinetic deuterium isotope effects have been measured as a functi

68 15N isotope effects and solvent deuterium isotope effects have been measured for the hyd

69 ms and in the P-O(R) ester bond, and solvent deuterium isotope effects, have been measured for the hy

70 Secondary deuterium isotope effects (IEs) on acidities have been a

71 Secondary deuterium isotope effects (IEs) on basicities of isotopo

72 liable temperature dependence of the kinetic deuterium isotope effect in a 1,5-hydrogen shift, the ti

73 From the deuterium isotope effect in the FVP of PhCD(2)CH(2)OPh,

74 The overall deuterium isotope effect in the presence of AdoCbi-GDP (

75 Here, we show that the conformational deuterium isotope effect, in combination with Saunders'

76 Modest substrate deuterium isotope effects indicate that hydride transfer

77 lack of significant kinetic and partitioning deuterium isotope effects indicates that the isomerizati

78 ind that the magnitude of the conformational deuterium isotope effect is 252.1, 28.3, and 7.1 J/mol (

79 The solvent kinetic deuterium isotope effect is also unity at low AcCoA, but

80 e results it is estimated that the aldehydic deuterium isotope effect is approximately 1.9 after form

81 A solvent deuterium isotope effect is observed for the azide-accel

82 Remarkably, a primary hydrogen-deuterium isotope effect is readily detected at the sing

83 This study demonstrates that (a) the deuterium isotope effect is useful in assessing the bind

84 A large primary deuterium isotope effect (k(H)/k(D) = 18.9 at 295 K) ind

85 traction mechanism, in line with the kinetic deuterium isotope effects, k(H)/k(D), of 2.0 and 3.1 mea

86 These experiments yield a deuterium isotope effect, kH/kD approximately 3 for ABLM

87 Here we report results of kinetic deuterium isotope effect (KIE) measurements on ETp throu

88 CHO showed a high apparent intrinsic kinetic deuterium isotope effect (KIE), >/=8.

89 On the basis of (13)C and deuterium isotope effects, L-ribulose-5-phosphate 4-epim

90 Theoretical deuterium isotope effects match well with those from exp

91 microM, respectively, and a primary kinetic deuterium isotope effect of 1.3 and 1.8 on V/ K AcCoA an

92 Furthermore, a deuterium isotope effect of 1.9 and a linear proton inve

93 )]farnesylcysteine as a substrate, a primary deuterium isotope effect of 2 was observed on the steady

94 of a pL (L being H or D)-independent solvent deuterium isotope effect of 2.

95 An intermolecular deuterium isotope effect of 2.0-2.5 was observed under s

96 respectively, giving a calculated intrinsic deuterium isotope effect of 3.3 +/- 0.9, consistent with

97 eavage of the oxo linkage exhibits a solvent deuterium isotope effect of 3.6, but a similar effect is

98 A substrate deuterium isotope effect of 32 was measured for the k(ca

99 icroM, respectively, while a primary kinetic deuterium isotope effect of about 1.4 was obtained on V,

100 In the steady-state a solvent deuterium isotope effect of about 2 was measured on (V/K

101 ocus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed i

102 is asynchronous, however, with an intrinsic deuterium isotope effect of approximately 5 and a 13C is

103 A solvent deuterium isotope effect of three to seven was observed

104 Reaction of CD3O* showed a deuterium isotope effect of ~6.5.

105 Smaller deuterium isotope effects of 1.03-1.04 from dideuteratio

106 Using 1D (13)C NMR, we have found deuterium isotope effects of 1.043 +/- 0.004, 1.027 +/-

107 Solvent deuterium isotope effects of 1.3 and 2.6 on V/K and V(ma

108 Noncompetitive kinetic deuterium isotope effects of 2-3 were measured for all O

109 Intermolecular non-competitive deuterium isotope effects of 3.1-3.8 were measured for k

110 omparison of the pH profiles and the solvent deuterium isotope effects of A. thaliana GS and the Arg-

111 50) 2E1 substrates are known to show kinetic deuterium isotope effects of approximately 5 on Km (DK =

112 SB hydrolysis in the dark state and a strong deuterium isotope effect on dark state SB hydrolysis.

113 There was no selective solvent deuterium isotope effect on enzyme catalysis.

114 ansfer and the presence of a kinetic solvent deuterium isotope effect on hydride transfer.

115 n is the finding that [3-(2)H]-10 exhibits a deuterium isotope effect on inactivation of 3.3, suggest

116 A small primary deuterium isotope effect on k(cat) (1.5) and a slightly

117 The solvent deuterium isotope effect on k(cat) is 2.7 +/- 0.2 and 1.

118 A small solvent kinetic deuterium isotope effect on k(cat) of 1.76 +/- 0.25, ind

119 the origin of the relatively small substrate deuterium isotope effect on k(cat)/K(m)(O(2)).

120 The primary deuterium isotope effect on K1 for flavin reduction at h

121 The primary deuterium isotope effect on Kcat for cytochrome c reduct

122 The absence of a solvent deuterium isotope effect on product distribution in the

123 The deuterium isotope effect on the consumption of [1-2H,1,2

124 An analysis of the deuterium isotope effect on the two rapid-mix reaction s

125 oxidation and reduction rates for Y(Z), the deuterium isotope effect on these rates, and the Y(Z)* -

126 The deuterium isotope effect on this hydrogen bridge is 2.2

127 , lungs, kidneys, and spleen showed a robust deuterium isotope effect on uptake, IQ, k3, and lambdak3

128 The deuterium isotope effect on V/K(aspartate) is pH indepen

129 Secondary beta deuterium isotope effects on acidity constants of ammoni

130 Secondary beta-deuterium isotope effects on amine basicities are measur

131 Solvent deuterium isotope effects on binding were observed for b

132 High intermolecular non-competitive kinetic deuterium isotope effects on both kcat and kcat/Km, from

133 The deuterium isotope effects on k(cat) and k(cat)/K(m) in n

134 Significant primary deuterium isotope effects on kcat (Dkcat) and kcat/KPt (

135 Analysis of solvent deuterium isotope effects on NCS-chrom degradation and D

136 Deuterium isotope effects on nitrogen and proton are of

137 imental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase re

138 Interestingly, double-deuterium isotope effects on the Cys130Ser mutant also s

139 hy, (1)H NMR, site-directed mutagenesis, and deuterium isotope effects on the geometry and chemical s

140 Primary deuterium [NADPH(D)] and solvent deuterium isotope effects on the kinetic parameters were

141 The primary deuterium isotope effects on the V(max) and the V/K(lact

142 (R)-NADPD but not (S)-NADPD showed kinetic deuterium isotope effects on V and V/K of about 1.9 and

143 Large primary deuterium isotope effects on V and V/K using 3-APADPH in

144 Larger primary deuterium isotope effects on V and V/K were observed for

145 Solvent deuterium isotope effects on V and V/K(MgHIc) were near

146 th substrate and coenzyme, together with the deuterium isotope effects on Vmax and Vmax/Km, have been

147 Also, a deuterium-isotope effect on the burst rate constant of p

148 The primary deuterium-isotope effect on V/K6PG for both enzymes is c

149 Deuterium-isotope effects on V, V/KNADP, and V/K6PG are

150 d by solvation changes that generate solvent deuterium isotope effects originating from hydrogen ion

151 tly different isotope scrambling and kinetic deuterium isotope effect patterns.

152 A product deuterium isotope effect (PIE) of 1.0 was determined as

153 A similar experiment involving a deuterium isotope effect previously returned the same vo

154 The combination of solvent and substrate deuterium isotope effects rules out solvent deuterium ex

155 Solvent deuterium isotope effect studies indicate that transfer

156 uted tetrahydropyridines, we have undertaken deuterium isotope effect studies on the substrate and in

157 Viscosity and solvent deuterium isotope effects studies suggest the isomerizat

158 The deuterium isotope effect study suggested that substituti

159 howed a pH 6 activity maximum but no kinetic deuterium isotope effect, suggesting protons are not tra

160 o acetaldehyde is characterized by a kinetic deuterium isotope effect that increases K(m) with no eff

161 Catalysis is modulated by a kinetic deuterium isotope effect that reduces the overall interc

162 00Lys mutant does not exhibit the very large deuterium isotope effects that are observed for homolysi

163 er, pressure increases the primary intrinsic deuterium isotope effect, the opposite of what was obser

164 C-clorgyline ((11)C-clorgyline-D2) using the deuterium isotope effect to assess binding specificity.

165 IE results and the lack of a kinetic solvent deuterium isotope effect together provide strong evidenc

166 Deuterium isotope effects, typical of a concerted metala

167 The primary deuterium isotope effect using L-serine 2-D is one on (V

168 , (iv) the lack of a non-competitive kinetic deuterium isotope effect, (v) the lack of a kinetic burs

169 atter compound, a substantial intermolecular deuterium isotope effect was observed for N-demethylatio

170 A normal deuterium isotope effect was observed for the hydrogenol

171 A prominent secondary four-bond hydrogen/deuterium isotope effect was observed from proton NMR at

172 A pronounced deuterium isotope effect was observed in alternating d(G

173 A primary deuterium isotope effect was observed under single-turno

174 homoisocitrate as the substrate, no primary deuterium isotope effect was observed, and a small (13)C

175 % of that for the wild-type complex, and the deuterium isotope effect was significantly decreased.

176 he solvent, primary, secondary, and multiple deuterium isotope effects were most consistent with a ch

177 p is not rate-limiting, while larger primary deuterium isotope effects were observed for poor ketoaci

178 rs at 107.5 +/- 3 s-1 and exhibits a 10-fold deuterium isotope effect when (4R)-[2H]NADH is substitut

179 k of a kinetic burst, and (vi) the lack of a deuterium isotope effect when the reaction was initiated

180 ments also indicate that there is no primary deuterium isotope effect with L-serine 2-D.

181 mutations increase the value of the primary deuterium isotope effect with tryptophan as a substrate,

182 re observed in the magnitudes of the primary deuterium isotope effects with NADPD, consistent with de