ライフサイエンスコーパス: diacylglycerol kinase

1 r and generation of a functional oligomer of diacylglycerol kinase.

2 s of the polytopic integral membrane protein diacylglycerol kinase.

3 sequence similarity to the Escherichia coli diacylglycerol kinase.

4 homotrimer of the integral membrane protein, diacylglycerol kinase.

5 lical membrane protein from Escherichia coli diacylglycerol kinase.

6 ro, consistent with it acting primarily as a diacylglycerol kinase.

7 so applied to the integral membrane protein, diacylglycerol kinase A where the structures determined

8 rol, suggesting that FBGC formation involves diacylglycerol kinase activation.

9 Cells lacking diacylglycerol kinase activity (e.g. dgk1Delta mutation)

10 work, we provide evidence that DGK1-encoded diacylglycerol kinase activity is required to convert tr

11 Inhibition of diacylglycerol kinase activity results in the detachment

12 Diacylglycerol kinase activity was stimulated by major m

13 dicated by analyses of DGK1 mRNA, Dgk1p, and diacylglycerol kinase activity.

14 ctions of Dgk1p were specifically due to its diacylglycerol kinase activity.

15 the CTP transferase domain caused a loss of diacylglycerol kinase activity.

16 he CTP transferase domain was sufficient for diacylglycerol kinase activity.

17 In this paper, we show that diacylglycerol kinase alpha (DGK-alpha), which phosphory

18 Here we show that depletion of diacylglycerol kinase alpha (DGKalpha), a kinase devoid

19 The data presented point to diacylglycerol kinase alpha as a novel but selective tar

20 Diacylglycerol kinase alpha is an 82-kDa DGK isoform tha

21 r Aggr1 and Aggr2, respectively, include the diacylglycerol kinase alpha subunit gene (Dagk1) and the

22 on Ras-Erk1/2 signaling and is inhibited by diacylglycerol kinases alpha and zeta.

23 Diacylglycerol kinase-alpha (DGK-alpha) was more highly

24 iacylglycerol kinase-epsilon (DGKepsilon) or diacylglycerol kinase-alpha (DGKalpha) knockout mice wer

25 he growth of wild type cells, indicated that diacylglycerol kinase also functions to alleviate diacyl

26 recently described radioactive method using diacylglycerol kinase and 50 times more sensitive than a

27 ogenous ceramide measured by 32P labeling by diacylglycerol kinase and a 4-fold increase in ceramide

28 ide elevations were detected concurrently by diacylglycerol kinase and electrospray tandem mass spect

29 yl caged diC(8) is biologically inert toward diacylglycerol kinase and protein kinase C in vitro and

30 ition is primarily because of the failure of diacylglycerol kinase, and are consistent with the propo

31 In contrast, GOA-1 (Goalpha) and DGK-1 (diacylglycerol kinase) appear to negatively regulate syn

32 Diacylglycerol kinases are important mediators of lipid

33 We identify the DGK-3 diacylglycerol kinase as a thermal memory molecule that

34 ramide were detected using the enzymatic 1,2-diacylglycerol kinase assay or the non-enzymatic o-phtha

35 Ceramide levels were quantified by the diacylglycerol kinase assay using thin-layer chromatogra

36 Ceramide levels were quantified by the diacylglycerol kinase assay using thin-layer chromatogra

37 ceramide generation (as measured by in vitro diacylglycerol kinase assay) in LLC-PK1 cells.

38 Using diacylglycerol kinase assays to quantify ceramide, we fo

39 In particular, forced expression of diacylglycerol kinase beta abrogated DAG accumulation at

40 of DAG kinases or expression of an inactive diacylglycerol kinase beta mutant increased the proporti

41 Consistent with the reported activation of diacylglycerol kinase by alpha-tocopherol, the diacylgly

42 Diacylglycerol kinase catalyses the ATP-dependent conver

43 ids were identical in DgkB and the mammalian diacylglycerol kinase catalytic cores.

44 random equilibrium mechanism, implying that diacylglycerol kinase catalyzes direct phosphoryl transf

45 t Saccharomyces cerevisiae, the DGK1-encoded diacylglycerol kinase catalyzes the CTP-dependent phosph

46 d enzyme stalling provided evidence that the diacylglycerol kinase could desorb from these vesicles,

47 Prokaryotic diacylglycerol kinase (DAGK) and undecaprenol kinase (UD

48 Prokaryotic diacylglycerol kinase (DAGK) functions as a homotrimer o

49 The integral membrane enzyme diacylglycerol kinase (DAGK) has long been thought to re

50 Escherichia coli diacylglycerol kinase (DAGK) is a 13.2 kDa enzyme which

51 Escherichia coli diacylglycerol kinase (DAGK) is a homotrimeric helical i

52 f the homotrimeric integral membrane protein diacylglycerol kinase (DAGK) is addressed.

53 ng of the trimeric integral membrane protein diacylglycerol kinase (DAGK) is documented and a method

54 due, Tyr16, of the integral membrane protein diacylglycerol kinase (DAGK) plays a critical role in th

55 Escherichia coli diacylglycerol kinase (DAGK) represents a family of inte

56 this contribution, the catalytic activity of diacylglycerol kinase (DAGK) was measured following reco

57 lubilized membrane protein, Escherichia coli diacylglycerol kinase (DAGK), and to sustain its native

58 terface of the homotrimeric membrane protein diacylglycerol kinase (DAGK).

59 nt of cellular lipid extracts with bacterial diacylglycerol kinase (DAGK).

60 Diacylglycerol kinases (DagKs) are key enzymes in lipid

61 known genes are disrupted at Xp11.2, whereas diacylglycerol kinase delta (DGKD) is disrupted at 2q37.

62 t de-ubiquitination of EGFR was regulated by diacylglycerol kinase delta (DGKdelta), a lipid kinase t

63 This pathway is regulated by diacylglycerol kinase delta (DGKdelta).

64 transcriptional activity of HIF-1alpha in a diacylglycerol kinase-dependent manner.

65 ansduction cascade rapidly increases nuclear diacylglycerol kinase (DGK) activity and PA production.

66 Diacylglycerol kinase (DGK) activity was important for t

67 Using the key anergy target gene diacylglycerol kinase (DGK) alpha as a focal point, we i

68 for expressing either full-length Sus scrofa diacylglycerol kinase (DGK) alpha or the catalytic domai

69 Diacylglycerol kinase (DGK) catalyzes the conversion of

70 mplex lipids and in intracellular signaling; diacylglycerol kinase (DGK) catalyzes the phosphorylatio

71 Diacylglycerol kinase (DGK) converts DG into phosphatidi

72 gating via sequential activation of PKC and diacylglycerol kinase (DGK) coupled with upregulation of

73 T cells that lacked one or both isoforms of diacylglycerol kinase (dgk) expressed highly in T cells,

74 The diacylglycerol kinase (DGK) family of enzymes plays crit

75 Diacylglycerol kinase (DGK) from Escherichia coli contai

76 The integral membrane protein diacylglycerol kinase (DGK) from Escherichia coli has be

77 active site of the trimeric membrane protein diacylglycerol kinase (DGK) from Escherichia coli.

78 nsposon was inserted into the 3' region of a diacylglycerol kinase (dgk) gene.

79 We found that diacylglycerol kinase (DGK) inhibitor II (R59949) decrea

80 For retinal blood flow studies, diacylglycerol kinase (DGK) inhibitor R59949, at various

81 Diacylglycerol kinase (DGK) plays a key role in cellular

82 Diacylglycerol kinase (DGK) terminates diacylglycerol (D

83 Here we report the identification of a diacylglycerol kinase (DGK) which also associates with b

84 e we describe a solution to this problem for diacylglycerol kinase (DGK), an integral membrane protei

85 pathways generate PA: phospholipase D (PLD), diacylglycerol kinase (DGK), and lysophosphatidic acid a

86 of either phospholipase D (PLD1 and PLD2) or diacylglycerol kinase (DGK), two enzyme classes involved

87 In this study, we characterized the role of diacylglycerol kinase (DGK), which phosphorylates DAG to

88 orylation to phosphatidic acid, catalyzed by diacylglycerol kinase (DGK).

89 to phosphatidic acid, which is catalyzed by diacylglycerol kinase (DGK).

90 (PA) generated by phospholipase D (PLD) and diacylglycerol kinase (DGK).

91 Diacylglycerol kinase (DGK)zeta is a negative regulator

92 udy, we demonstrate that genetic deletion of diacylglycerol kinase (DGK)zeta, a negative regulator of

93 (beta2AR), the peptide transporter (PepTSt), diacylglycerol kinase (DgkA), the alginate transporter (

94 This gene encodes the diacylglycerol kinase DGKepsilon, which is an intracellu

95 Diacylglycerol kinases (DGKs) catalyze the ATP-dependent

96 Diacylglycerol kinases (DGKs) inhibit diacylglycerol (DA

97 Diacylglycerol kinases (DGKs) phosphorylate diacylglycer

98 Diacylglycerol kinases (DGKs) phosphorylate diacylglycer

99 Diacylglycerol kinases (DGKs) terminate signalling from

100 beta-Arrestins physically interact with diacylglycerol kinases (DGKs), enzymes that degrade DAG.

101 G), levels of which are tightly regulated by diacylglycerol kinases (DGKs), is a lipid mediator linke

102 metabolism in T cell anergy, we manipulated diacylglycerol kinases (DGKs), which are enzymes that te

103 Diacylglycerol kinases (DGKs), which negatively regulate

104 activity and substrate affinity of a soluble diacylglycerol kinase, DGKtheta.

105 h include loss of the GOA-1 G(o)alpha, DGK-1 diacylglycerol kinase, EAT-16 G protein gamma subunit-li

106 Saccharomyces cerevisiae DGK1 gene encodes a diacylglycerol kinase enzyme that catalyzes the formatio

107 complement defect, some have either impaired diacylglycerol kinase epsilon (DGKepsilon) activity, cob

108 Human diacylglycerol kinase epsilon (hDGK epsilon) displays hi

109 s-of-function mutations in the gene encoding diacylglycerol kinase epsilon result in atypical hemolyt

110 ansformed fibroblasts from wild-type or from diacylglycerol kinase-epsilon (DGKepsilon) or diacylglyc

111 cases; however, mutations in the non-C gene diacylglycerol kinase-epsilon have been described recent

112 DGKE, encoding diacylglycerol kinase-epsilon, has not been implicated i

113 genome-wide association studies have linked diacylglycerol kinase eta (DGKeta) to bipolar disorder (

114 Several lines of evidence indicate that the diacylglycerol kinase eta (DGKH) gene is implicated in t

115 The mechanism of folding of Escherichia coli diacylglycerol kinase from a partially denatured state i

116 temperature-sensitive Sec14p, expression of diacylglycerol kinase from Escherichia coli further impa

117 Unlike the diacylglycerol kinases from bacteria, plants, and animal

118 iation rate of the trimeric membrane enzyme, diacylglycerol kinase, from Escherichia coli as a proxy

119 this clone maps to 12q13, centromeric to the diacylglycerol kinase gene (DAGK) at 12q13.3.

120 This study suggests a novel role for diacylglycerol kinase in the mechanism of macrophage fus

121 tic activity of an integral membrane enzyme, diacylglycerol kinase, in the complete absence of additi

122 ells with induced expression of DGK1-encoded diacylglycerol kinase indicated that alteration in phosp

123 peroxide was suppressed by the loss of Dgk1 diacylglycerol kinase, indicating that the underpinning

124 acylglycerol kinase by alpha-tocopherol, the diacylglycerol kinase inhibitor R59022 completely abroga

125 This conversion was sensitive to the diacylglycerol kinase inhibitor R59949 and explains the

126 Consistent with this view, diacylglycerol kinase is identified as a key enzyme requ

127 s work, we showed that a functional level of diacylglycerol kinase is regulated by the Reb1p transcri

128 This study shows that diacylglycerol kinase is selectively activated by ANCA a

129 While a number of diacylglycerol kinase isoforms have been implicated in c

130 P is mostly associated with one unique mRNA: diacylglycerol kinase kappa (Dgkkappa), a master regulat

131 Our data suggest that diacylglycerol kinases limit the extent of NADPH oxidase

132 We thus identified diacylglycerol kinase-mediated phosphatidic acid biosynt

133 To identify the diacylglycerol kinase mediating this effect, we knocked

134 ssay is more specific than the commonly used diacylglycerol kinase method because the ubiquitous lipi

135 to that of the widely used Escherichia coli diacylglycerol kinase method.

136 Diacylglycerol kinase modulates the levels of diacylglyc

137 els were reduced approximately 5-fold in the diacylglycerol kinase mutant (rdgA), but basal PLC activ

138 PhgC has a domain with similarity to diacylglycerol kinases of eukaryotes and is the first de

139 , the liver can synthesize PAs by activating diacylglycerol kinase or phospholipase D, both of which

140 Increasing or decreasing levels of diacylglycerol kinase or phospholipase D-enzymes that pr

141 atidylinositol 3-kinase, but not p38 kinase, diacylglycerol kinase, or hypoxia-inducible factor-1alph

142 dicate that the Reb1p-mediated regulation of diacylglycerol kinase plays a major role in its in vivo

143 dioctanoylphosphatidic acid by an endogenous diacylglycerol kinase present in the cell-free reaction

144 es arachidonic acid via phospholipase D2 and diacylglycerol kinase rather than phospholipase A2.

145 vity only indirectly, not by influencing the diacylglycerol kinase reaction itself.

146 lication of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes.

147 In this pathway, RHO-1 inhibits diacylglycerol kinase, resulting in an increase in diacy

148 However, when PI 4-kinase IIIbeta, diacylglycerol kinase, Rho, or Rho-kinase was blocked, a

149 focuses upon misfolding of a mutant form of diacylglycerol kinase (s-DAGK), a 40 kDa homotrimeric pr

150 While diacylglycerol kinase's kcat/Km is modest compared with

151 lipid bilayer normally plays in maintaining diacylglycerol kinase's structure and in facilitating ca

152 We show that Dgk1p is a unique diacylglycerol kinase that uses CTP, instead of ATP, to

153 lipid vesicles to partially purify a soluble diacylglycerol kinase, then studied the relation between

154 Overexpression of diacylglycerol kinase-theta inhibited insulin signaling

155 by overexpression of phospholipase D1/D2 or diacylglycerol kinase-theta was always accompanied by di

156 , MgCl2, and ATP on the ability of a soluble diacylglycerol kinase to bind to 100-nm lipid vesicles.

157 es of the activity of the partially purified diacylglycerol kinase toward vesicle-associated diacylgl

158 tified recessive mutations in DGKE (encoding diacylglycerol kinase varepsilon) that co-segregated wit

159 Analysis of the membrane protein diacylglycerol kinase was accomplished using the combina

160 latory effects of phosphatidylserine on many diacylglycerol kinases, we examined the effects of vario

161 nd substrate specificity of Escherichia coli diacylglycerol kinase were examined.

162 ed variants of the integral membrane protein diacylglycerol kinase were measured.

163 Loss of the DGK1-encoded diacylglycerol kinase, which converts diacylglycerol to

164 Deletion of the DGK1-encoded diacylglycerol kinase, which counteracts PA phosphatase

165 ation product of vlmJ exhibits similarity to diacylglycerol kinases, while the translation product of

166 We demonstrate here that diacylglycerol kinase zeta (DGKzeta) interacts, via its

167 -reactive T cells deficient in the regulator diacylglycerol kinase zeta (DGKzeta) with or without PD-

168 Previous studies have shown that diacylglycerol kinase zeta (DGKzeta), which converts dia

169 Diacylglycerol kinase zeta (DGKzeta), which phosphorylat

170 genetic ablation of a negative regulator of diacylglycerol kinase zeta increased the suppressive abi

171 erol (DAG), and its levels are influenced by diacylglycerol kinase-zeta (DGKzeta), which metabolizes