ライフサイエンスコーパス: diaphanous

1 on in a human homolog of the Drosophila gene diaphanous.

2 nd promoted junction integrity by activating Diaphanous.

3 E with the intracellular effector, mammalian diaphanous 1 or DIAPH1.

4 h the effector protein, mDia1 (for mammalian Diaphanous 1).

5 Loss of the formin mammalian Diaphanous 1, a regulator of linear actin polymerization

6 -related gene in leukocytes alpha) and mouse diaphanous 1, autoinhibition regulates a novel membrane

7 t the RAGE cytoplasmic domain interacts with Diaphanous-1 (Dia-1) both in vitro and in vivo.

8 Conversely, the actin-nucleating formins, Diaphanous-1 (DIA1) and Formin-like-1 (FMNL1), did not a

9 lopodia formation via the Arp2/3 complex and Diaphanous 2 (Diaph2).

10 ubules, the activation of RhoA-GTP-dependent diaphanous 2 was observed, with no significant activatio

11 tidylinositol 3-kinase (PI3-K), Rho-GTPases, Diaphanous-2 (Dia-2), Ezrin, protein kinase C-zeta, extr

12 adhesion, suggesting a common mechanism for Diaphanous action during morphogenesis.

13 e hypothesized that differing Enabled and/or Diaphanous activity drives these differences.

14 rocesses, we generated embryos deficient for Diaphanous and analyzed three cell-cycle-regulated actin

15 The actin elongation factors Diaphanous and Enabled both promote barbed-end actin pol

16 n with quantitative approaches revealed that Diaphanous and Enabled each regulate filopodial behavior

17 ent of the septin Peanut and distribution of Diaphanous and F-actin at furrows.

18 FHOS has sequence homology to Diaphanous and Formin proteins within the Formin Homolog

19 in furrow-initiation mutants of RhoGEF2 and Diaphanous and in furrow-progression mutants of Anillin.

20 w that this actomyosin flow was regulated by Diaphanous and ROCK and that it elicited a wave of apica

21 -actin, the centralspindlin complex, formin (diaphanous), and profilin (chickadee) are required to st

22 lgi apparatus with the formin-family protein Diaphanous, and loss of either isoform perturbs cell cyc

23 ization of the Arp2/3 complex and the formin Diaphanous, and mutations in diaphanous or capping prote

24 inhibition depends upon the presence of the diaphanous auto-regulatory domain (DAD) C-terminal to FH

25 xamine previously identified coiled-coil and Diaphanous auto-regulatory domain sequences.

26 nhibited through intramolecular binding of a Diaphanous autoinhibitory domain (DAD) to a conserved N-

27 a role in nucleation was identified for the Diaphanous autoinhibitory domain (DAD), which is C-termi

28 GTPase binding domain (GBD) and a C-terminal Diaphanous autoinhibitory domain (DAD, ).

29 ition, INF2 binds actin monomers through its diaphanous autoregulatory domain (DAD) that resembles a

30 ous inhibitory domain (DID) and a C-terminal diaphanous autoregulatory domain (DAD).

31 yl terminus of Bni1 that are adjacent to its diaphanous autoregulatory domain (DAD).

32 itory domain (DID) and the carboxyl-terminal diaphanous autoregulatory domain (DAD).

33 predicting partial truncation of the DIAPH1 diaphanous autoregulatory domain.

34 ding of a Diaphanous inhibitory domain and a Diaphanous autoregulatory domain.

35 Recently, the C-terminal diaphanous-autoregulatory domain (DAD) and the C terminu

36 se-binding domain (mDiaN) and the C-terminal Diaphanous-autoregulatory domain (DAD).

37 d Cdc42, or the regulatory formins dDAAM and Diaphanous caused mislocalization of Zipper and induced

38 interacting with microfilaments, chickadee, diaphanous, Cdc42, quail, spaghetti-squash, zipper, and

39 We characterized Diaphanous (Dia) and Enabled (Ena) as a model, using com

40 Furthermore, we show that the formin Diaphanous (DIA) functions with APC2 in this process.

41 Diaphanous (Dia)-related formins (DRFs) coordinate cytos

42 phila leading-edge (LE) cells to explore how Diaphanous (Dia)-related formins and Ena/VASP proteins c

43 lex, is also capable of interacting with the Diaphanous (Dia)-related formins in the absence of Wave.

44 ables generated by the formin-family protein Diaphanous (Dia).

45 To gain insight into the function of Diaphanous during cytokinesis and explore its role in ot

46 deriving new insights into (a) the roles of Diaphanous, Enabled, and Capping protein in regulating f

47 A novel member of the Formin/Diaphanous family of proteins was cloned and characteriz

48 ntractile ring components, the Rho effectors diaphanous formin and myosin-II.

49 lymerization that involves activation of the Diaphanous formin Dia.

50 mDia1 is a member of the Diaphanous formin subfamily (Dia), whose members contain

51 is upstream of both myosin-II activation and diaphanous formin-mediated filamentous actin (f-actin) a

52 ical progenitors, unravel novel functions of Diaphanous formins and add insights into the pathobiolog

53 in tubular organs of Drosophila, the role of Diaphanous formins at the final stages of secretion appe

54 A subset of formins termed "diaphanous formins" are regulated by autoinhibition thro

55 nduced cellular activation, sequestration of Diaphanous formins, and clustering of the receptor.

56 Diaphanous formins, including mDia1, mDia2, and mDia3 in

57 We tested this hypothesis by reducing Diaphanous function, revealing striking roles in stabili

58 The diaphanous gene is the founding member of a family of Di

59 We found that Diaphanous has a dynamic expression pattern consistent w

60 Our findings thus indicate that Diaphanous has a role in actin cytoskeleton organization

61 The Drosophila Formin Homology (FH) protein Diaphanous has an essential role during cytokinesis.

62 The genes that have been identified encode diaphanous (HDIA1), alpha-tectorin (TECTA), the transcri

63 ns during cell shape change, suggesting that Diaphanous helps coordinate adhesion and contractility o

64 activating RhoA and the downstream effector Diaphanous homolog 1 (Dia1).

65 1) confirmed rs318125, downstream of DIAPH2 (diaphanous homolog 2 (Drosophila)) (Pallele=0.010, Pgeno

66 We have identified Diaphanous homolog 3 (DIAPH3) as the gene responsible fo

67 Diaphanous homologue 1 (DIAPH1) is a Rho effector protei

68 The diaphanous homologue Diaph3 (aka mDia2) is a major regul

69 We identified diaphanous in a screen for genes that are necessary for

70 previous results that identified a role for Diaphanous in apical secretion in tubular organs of Dros

71 f-function approaches to examine the role of Diaphanous in Drosophila morphogenesis.

72 the dia mutant phenotype reveals a role for Diaphanous in recruitment of myosin II, anillin and Pean

73 ession of an activated version of the formin Diaphanous, induced strong overgrowth in Drosophila imag

74 used by RhoB/mammalian homolog of Drosophila diaphanous-induced actin polymerization and RhoA/Rho kin

75 on through interaction between an N-terminal diaphanous inhibitory domain (DID) and a C-terminal diap

76 se binding domain (GBD) that encompasses the diaphanous inhibitory domain (DID) and the carboxyl-term

77 toinhibitory interaction with the N-terminal diaphanous inhibitory domain (DID).

78 sisting of the GTPase-binding region and the diaphanous inhibitory domain (G-DID), thought to mediate

79 hanism involving intramolecular binding of a Diaphanous inhibitory domain and a Diaphanous autoregula

80 These mutations, all within the diaphanous inhibitory domain of INF2, segregate with FSG

81 in spanning residues 129-369 (called DID for diaphanous inhibitory domain) is sufficient for auto-inh

82 ined six-helix bundle, from which extend two Diaphanous inhibitory domains (DIDs) composed of five ar

83 The second (residues 334-821) covers the Diaphanous inhibitory-dimerization-coiled coil domains,

84 identified a new interaction between Nef and diaphanous interacting protein (DIP), a recently describ

85 ll cytoskeleton, we propose a model in which Diaphanous links receptor tyrosine phosphatase signaling

86 ion studies of wild-type embryos reveal that Diaphanous localizes to the site where the metaphase fur

87 Mammalian Diaphanous (mDia) family formins are Rho-directed effect

88 nd CYK-1, the sole ortholog of the mammalian diaphanous (mDia) family of formins.

89 n Rho and its effector, mammalian homolog of Diaphanous (mDia), in migrating cells, but factors respo

90 Mammalian Diaphanous (mDia)-related formins and the N-WASP-activat

91 Rho GTPase-effector mammalian diaphanous (mDia)-related formins assemble nonbranched a

92 of small-molecule agonists of the mammalian Diaphanous (mDia)-related formins, which act downstream

93 ell characterized, the role of the mammalian Diaphanous (mDia)-related formins, which both nucleate a

94 in parallel, induces a switch from Arp2/3 to Diaphanous-mediated cortical actin nucleation that depen

95 Here, we demonstrate that diaphanous mutations perturb synaptic growth at the NMJ.

96 and the formin Diaphanous, and mutations in diaphanous or capping protein beta enhance abl phenotype

97 We have identified mDia1/Drf1 (mammalian Diaphanous or Diaphanous-related formin 1 protein) as a

98 Importantly, loss of Capping proteins and Diaphanous overexpression did not significantly affect c

99 iveness is primarily Enabled driven, whereas Diaphanous plays the primary role in the AS, and reveal

100 xpression of a constitutively active form of diaphanous protein in the auditory organ of Drosophila m

101 The diaphanous protein is a profilin ligand and target of Rh

102 Diaphanous protein is present both presynaptically and p

103 its furrow-extension phenotypes, Peanut and Diaphanous recruitment, and F-actin organization.

104 Knockdown of the actin nucleating protein Diaphanous Related Formin 3 (DRF3/Dia2) by RNA interfere

105 Mammalian diaphanous-related (mDia) formins act as Rho GTPase effe

106 Mammalian Diaphanous-related (mDia) formins are well known for the

107 mall GTP-binding proteins activate mammalian diaphanous-related (mDia) formins by directly binding an

108 The diaphanous-related formin (DRF) proteins have been ident

109 We have examined the role of the mouse Diaphanous-related formin (DRF) Rho GTPase binding prote

110 on of DIAPH1, which encodes the Rho-effector diaphanous-related formin 1 (DIAPH1), as a candidate gen

111 The murine mammalian Diaphanous-related formin 1 (mDia1) was identified as a

112 and physically interacted with the mammalian diaphanous-related formin 1 (mDia1), a downstream effect

113 We identified mammalian diaphanous-related formin 1 (mDia1), a potent actin nucl

114 entified mDia1/Drf1 (mammalian Diaphanous or Diaphanous-related formin 1 protein) as a PKD2-interacti

115 ssociated activator of morphogenesis-1) is a diaphanous-related formin first studied as a novel dishe

116 Here, we show that the Diaphanous-related formin G (ForG) from the professional

117 Mammalian and fungal Diaphanous-related formin homology (DRF) proteins contai

118 tation in one copy of a tandemly duplicated, diaphanous-related formin is perfectly associated with s

119 that the Rif effector in this pathway is the Diaphanous-related formin mDia2.

120 iated activator of morphogenesis (DAAM) is a diaphanous-related formin protein essential for the regu

121 s gene is the founding member of a family of Diaphanous-related formin proteins (DRFs).

122 A mammalian Diaphanous-related formin, mDia1, localizes at the jagge

123 DIAPH1 encodes the mammalian Diaphanous-related formin, mDia1.

124 Elongation proteins, Diaphanous-related formin, VASP, and fascin are recruite

125 Mammalian Diaphanous-related formins (Drfs) act as Rho small GTPas

126 Diaphanous-related formins (DRFs) are key regulators of

127 However, the activation mechanism for these Diaphanous-related formins (DRFs) is not completely unde

128 Diaphanous-related formins (DRFs) regulate dynamics of u

129 INF2 interacts with diaphanous-related formins (mDia) and antagonizes mDia-m

130 In addition, the Diaphanous-related formins 1-3 (mDia1-3) localized to pr

131 In cultured cells, Diaphanous-related formins also regulate cell adhesion,

132 actin-filament assembly that is mediated by Diaphanous-related formins and activators of Arp2/3, res

133 The Diaphanous-related formins are effectors for Rho GTPases

134 Diaphanous-related formins are eukaryotic actin nucleati

135 Diaphanous-related formins, the best-known subclass, are

136 uses several downstream effectors, including Diaphanous, Rok, and Pkn, simultaneously to mediate its

137 However, redundancy in mammals and Diaphanous' role in cytokinesis limited analysis of whet

138 la melanogaster embryos, we demonstrate that Diaphanous, SCAR, and WASp play distinct but overlapping

139 The basal cytonemes required diaphanous, SCAR, Neuroglian and Synaptobrevin, and both

140 al functions of FH proteins, we propose that Diaphanous serves as a mediator between signaling molecu

141 Genetic loss-of-function conditions for diaphanous, shibire, neuroglian, and capricious perturbe

142 We used constitutively active Diaphanous to examine its roles in morphogenesis and its

143 Male-sterile alleles of chickadee and diaphanous, which are deficient in germ cells, exhibit s

144 Rho pathway, such as pebble, racGAP50C, and diaphanous, which had profound effects on furrowing but