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1 oduction of oxidants was examined using 2',7'dichlorofluorescin, a dye that forms a fluorescent produ
2 e basis of monitoring the oxidation of 2',7'-dichlorofluorescin by spectrofluorometry, flow cytometry
5 ble of oxidizing the fluorescent probe 2',7'-dichlorofluorescin (DCFH) also accumulate intracellularl
6 Single-cell fluorescence microscopy of 2',7'-dichlorofluorescin diacetate (DCF)-loaded cells showed t
7 A myocytes, oxidation of intracellular 2',7'-dichlorofluorescin diacetate (DCFH) dye increased under
8 irectly using confocal microscopy with 2',7'-dichlorofluorescin diacetate (DCFH) or using electron mi
10 oxidation-sensitive fluorescent probe 2',7'-dichlorofluorescin diacetate and laser-scanning confocal
11 n species as measured by a fluorescent 2',7'-dichlorofluorescin diacetate assay, and >85% reduction i
13 en species (ROS) (measured as an increase in dichlorofluorescin diacetate fluores-cence) and that sim
15 (intracellular oxidative burst activity with dichlorofluorescin diacetate staining) and adhesion (int
16 phosphorylation; the fluorochrome probe 2'7'-dichlorofluorescin diacetate was used to measure cytosol
17 brane-permeable dyes hydroethidine and 2',7'-dichlorofluorescin diacetate, respectively, were used to
18 eroxidation-sensitive fluorescent dye, 2',7'-dichlorofluorescin diacetate, was significantly elevated
20 lic oxidant levels were measured with 2', 7'-dichlorofluorescin diacetate; emissions of the oxidized
21 on per oxygen consumed, as measured by 2',7'-dichlorofluorescin fluorescence levels, is approximately
22 eneration (reactive oxygen species via 2',7'-dichlorofluorescin oxidation and nitric oxide [NO] via 4
24 During induction, ROS oxidation of the probe dichlorofluorescin (sensitive to H2O2) increased approxi
25 the fluorescence produced on oxidizing 2',7'-dichlorofluorescin, was significantly lowered in (R)-lip
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