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1 mine whether CTRP5 and MFRP are functionally dicistronic.
2 gene, and these two genes are reported to be dicistronic.
3 CAT-LUC RNA, a helper-dependent cDNA-encoded dicistronic analog of RSV genomic RNA in which the viral
4 B10 and pMHB11 constructs described here are dicistronic and encode genes for herbicide (bar) and spe
6 istronic and encodes Adh alone, the other is dicistronic and includes the open reading frames of both
7 more stringent studies, such as promoterless dicistronic and monocistronic DNA and dicistronic RNA te
14 pression of the downstream RDRP:GFP from the dicistronic construct CP-RDRP:GFP could not be detected.
18 essed from the first cistron from all of the dicistronic constructs introduced into the fly genome.
19 eader sequence or upstream of either CIRE in dicistronic constructs markedly increased their regulato
20 second cistron was not expressed in control dicistronic constructs that lacked these sequences or co
21 ne sh-ble as a reporter in monocistronic and dicistronic constructs, and consistently yielded higher
22 suggested from studies using the traditional dicistronic DNA assay that the expression of p27kip1 is
23 Considering the inevitable drawbacks of the dicistronic DNA assay, which could mislead a promoter ac
25 re, we confirm that ORF35-37 is functionally dicistronic during infection and demonstrate that mutati
26 1 RNA is exceptional because it is naturally dicistronic, encoding two proteins essential for retrotr
27 ample of a naturally occurring, functionally dicistronic eukaryotic mRNA whose translation is control
29 ogous RNA must be considered when IRES-based dicistronic expression vectors are being constructed.
33 and G-418 resistance to be transcribed as a dicistronic fusion mRNA under the control of the phospho
34 tween the CAT and lacZ coding sequences in a dicistronic gene and tested for IRES activity in transge
35 ressing the CFTR gene, the expression of the dicistronic gene can be detected by lacZ staining and fo
37 stem for dependable high-level expression of dicistronic genes in Chlamydomonas reinhardtii has been
38 on for instability of the genome is that the dicistronic genome was at least 108% larger than the wil
40 is unique amongst picornaviruses in having a dicistronic genome with internal ribosomal entry sites (
42 We designed a series of monocistronic and dicistronic GluR2 cDNA constructs that model the natural
43 h the nonstructural proteins, we constructed dicistronic HAV genomes in which a heterologous picornav
44 on-competent, revertant viruses rescued from dicistronic HRV-14 RNAs containing the poliovirus cre, o
45 rotein knockdown by RNA interference using a dicistronic lentivirus small hairpin (shRNA) delivery sy
46 mon exon into the intercistronic region of a dicistronic luciferase construct, yielded luciferase act
57 alyzed in helper-dependent monocistronic and dicistronic minireplicons which were complemented by a s
59 rotransposition, L1 RNA functions first as a dicistronic mRNA and then as a template for cDNA synthes
60 we found no evidence of IRES function when a dicistronic mRNA containing the eIF4G sequence was trans
65 ite, expression from the distal cistron of a dicistronic mRNA increased as a function of the length o
66 resent evidence that the accumulation of the dicistronic mRNA is controlled at the level of the 3' en
69 RES module in the intercistronic region of a dicistronic mRNA strongly enhances IRES activity in vari
71 This method uses a vector that encodes a dicistronic mRNA with a reporter gene (Renilla luciferas
72 en present in the intercistronic region of a dicistronic mRNA, indicating that the leader contains an
78 ure that uses a retroviral vector to express dicistronic mRNAs encoding enhanced green and cyan fluor
79 ient translation of a second URA3 cistron in dicistronic mRNAs in S. cerevisiae, thereby conferring u
80 RES enhanced translation of monocistronic or dicistronic mRNAs in vitro under competitive conditions,
81 and in vivo, as can be shown with artificial dicistronic mRNAs or with chimeric viral genomes in whic
83 d translation from uncapped monocistronic or dicistronic mRNAs to a greater extent than did eIFiso4F.
84 studies, a library of constructs expressing dicistronic mRNAs with the HIS3 gene as the second cistr
85 when tested in the intercistronic region of dicistronic mRNAs, both sequences were shown to contain
95 was present downstream of other sequences in dicistronic or chimeric transcripts, the RNAs were no lo
98 ddress this possibility, we have constructed dicistronic poliovirus replicons by substituting the EMC
99 on have been dispelled by constructing novel dicistronic poliovirus replicons with the gene order [PV
103 to complement the RNA replication defect in dicistronic PV or dicistronic luciferase replicons in vi
104 olic labeling of monocistronic mRNAs and the dicistronic readthrough RNAs that result when terminatio
105 serial passages with VV-P1 revealed that the dicistronic replicon was encapsidated more efficiently t
106 e of the poliovirus P1 region; the resulting dicistronic replicons are smaller than the wild-type pol
109 be promoters, mimic both types of element in dicistronic reporter assays by causing these cryptic rea
113 We compared the translational activity of dicistronic reporter transcripts containing the HCV IRES
118 omoter using more stringent methods, such as dicistronic RNA and promoterless dicistronic and monocis
119 us Cricket Paralysis virus contains a unique dicistronic RNA genome arrangement, encoding two main op
121 erless dicistronic and monocistronic DNA and dicistronic RNA tests, are required to safeguard any fut
122 We analyzed the translational activity of dicistronic RNA transcripts containing wild-type or muta
125 s hypothesis, we examined the translation of dicistronic RNAs containing upstream and downstream repo
127 ection of permissive FRhK-4 cells with these dicistronic RNAs failed to result in the rescue of infec
128 However, when capped and polyadenylated dicistronic RNAs were synthesized in vitro and transfect
133 osition 1049 (L(1049N) or L(1049D)) by using dicistronic subgenomic replicons and found that the alte
134 ed the RNA synthetic activities of mono- and dicistronic subgenomic replicons of RS virus by direct m
136 the feasibility and utility of 2A-containing dicistronic systems that rely on a strong conditional pr
138 he D. pseudoobscura cluster also generates a dicistronic transcript from a gene pair analogous to the
143 nd the apportionment of monocistronic versus dicistronic transcription of both genes appears to be su
144 thesis, we constructed plasmids containing a dicistronic transcriptional unit in which the HAV IRES w
145 e transfected cells with plasmids expressing dicistronic transcripts in which the upstream cistron en
148 uch as A-to-I editing, alternative splicing, dicistronic translation, and selenocysteine incorporatio
149 nce of these events suggests that the 7.5 kb dicistronic unit is suitable for constructing Leishmania
150 V40 T antigen and H-ras val12 oncogenes as a dicistronic unit separated by a poliovirus internal ribo
151 e can be expressed in mammalian cells from a dicistronic vector and has been purified using sequentia
153 n checked carefully to determine whether the dicistronic vector produces only the intended dicistroni
155 -myc, and demonstrated by transfections in a dicistronic vector system that it contains a potent inte
156 this concern, we created a new promoterless dicistronic vector to test the putative IRES derived fro
157 testing is carried out using a conventional dicistronic vector, Northern blot analyses might not be
160 , devised to rule out splicing in tests with dicistronic vectors, are not valid and why experiments w
164 R analysis confirmed strong induction at the dicistronic Wbeta locus and at four other phage loci in
165 evidence of recombination was observed at a dicistronic Wbeta locus, encoding putative bacterial cel
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