ライフサイエンスコーパス: dideoxynucleotide

1 this enzyme with a template, a primer, and a dideoxynucleotide.

2 tension reactions in place of the unmodified dideoxynucleotide.

3 oligonucleotide primers extended by a single dideoxynucleotide.

4 lls treated with the proteasome C2 antisense dideoxynucleotide.

5 of T7 DNA polymerase to discriminate against dideoxynucleotides.

6 le-nucleotide extension incorporating tagged dideoxynucleotides.

7 generated in one tube by using biotinylated dideoxynucleotides.

8 family of DNA polymerases, and inhibited by dideoxynucleotides.

9 ivity for deoxyribonucleotides over ribo- or dideoxynucleotides.

10 000-fold over the wild-type incorporation of dideoxynucleotides.

11 ing fragments in one tube using biotinylated dideoxynucleotides.

12 DNA polymerase and a pair of fluoresceinated dideoxynucleotides.

13 that do not discriminate between deoxy- and dideoxynucleotides.

14 nslation in the presence of trace amounts of dideoxynucleotides.

15 e presence of defined mixtures of deoxy- and dideoxynucleotides.

16 nucleic acid templates using 2'-amino-2',3'-dideoxynucleotide-5'-phosphorimidazolides.

17 we find that DNA substrates terminating in a dideoxynucleotide allow Mu transposase to hydrolyze a ta

18 ase activity was partially inhibited by both dideoxynucleotide and aphidicolin.

19 t variants complexed with deoxy-, ribo-, and dideoxynucleotides and a DNA substrate.

20 for the ability of pol gamma to incorporate dideoxynucleotides and D4T-MP.

21 Dideoxynucleotides and D4T-TP were utilized by pol gamma

22 yr951 to Phe renders the enzyme resistant to dideoxynucleotides and D4T-TP without compromising the a

23 la slightly increased discrimination against dideoxynucleotides and D4T-TP.

24 ng method using solid-phase capturable (SPC) dideoxynucleotides and MALDI-TOF mass spectrometry on sy

25 ng method using solid phase capturable (SPC) dideoxynucleotides and single base extension (SBE), name

26 ch as high-mobility ions (e.g., chloride and dideoxynucleotides) and template DNA on the injected amo

27 w fragment of E. coli pol I, is inhibited by dideoxynucleotides, and resistant to aphidicolin.

28 sion products terminated by the biotinylated dideoxynucleotides are released from the magnetic beads

29 use this slow phase was also observed with a dideoxynucleotide at the 3' end of the primer which prev

30 The presence of a dideoxynucleotide at the 3'-primer terminus allows captu

31 ed by using small synthetic RNAs ending with dideoxynucleotides at the 3' termini.

32 The fluorescent dye-labeled dideoxynucleotide automated DNA sequencing system has be

33 resistant to inhibition by chain-terminating dideoxynucleotides because gp5-Y526F is deficient in the

34 ch using solid phase capturable biotinylated dideoxynucleotides (biotin-ddNTPs) in single base extens

35 nsion (SBE) using CFET tags and biotinylated dideoxynucleotides (biotin-ddNTPs).

36 otide-blocked oligonucleotides, but not with dideoxynucleotide-blocked oligonucleotides.

37 n utilize either acyclonucleotide-blocked or dideoxynucleotide-blocked oligonucleotides.

38 e same motif not only affects sensitivity to dideoxynucleotides, but also greatly influences enzyme a

39 The dideoxynucleotide chain termination method was used to d

40 ls (PBMC) was compared to consensus sequence dideoxynucleotide chain terminator sequencing for detect

41 rse transcription-PCR and sequenced by using dideoxynucleotide chain terminators for evaluation of mu

42 Single-sided primer extension with dideoxynucleotide chain terminators is avoided, with the

43 ead-end complex was formed between HIV-1 RT, dideoxynucleotide chain-terminated primer, and DNA templ

44 es from each time point was sequenced by the dideoxynucleotide chain-termination method.

45 ated by the inclusion of any one of the four dideoxynucleotides, consistent with the presence of all

46 0 primary human lung cancers by using direct dideoxynucleotide cycle sequencing and compared with seq

47 Inefficient excision of dideoxynucleotides, D4T, AZT, and CBV from DNA predicts

48 e novel Survivor assay detects the unreacted dideoxynucleotides (ddNTPs) remaining or surviving in so

49 vel set of chemically cleavable biotinylated dideoxynucleotides, ddNTPs-N(3)-biotin, for the DNA poly

50 Deoxy- and dideoxynucleotides differ only in whether they have a hy

51 BV) are not well understood because standard dideoxynucleotide direct polymerase chain reaction (PCR)

52 ladders (i.e., removal of excess dye-labeled dideoxynucleotides, DNA template, and salts) prior to ge

53 alysis, flow cytometry, or complimentary DNA dideoxynucleotide fingerprinting or sequencing.

54 bined with the PCR products and biotinylated dideoxynucleotides for SBE to generate 3'-biotinylated e

55 phenylalanine in polymerase motif B reduced dideoxynucleotide inhibition by a factor of 5000 with on

56 e T7 DNA polymerase efficiently incorporates dideoxynucleotides into DNA, resulting in chain terminat

57 hod is described here in which a mass-tagged dideoxynucleotide is employed in the primer extension re

58 nt of incorporation of fluorescently labeled dideoxynucleotides is influenced by the methylated bases

59 is insensitive to inhibition by aphidicolin, dideoxynucleotides, or NaCl up to 50 mm.

60 t unligated intermediates were trapped using dideoxynucleotides revealed that there was no gap fillin

61 a coli thioredoxin, a primer-template, and a dideoxynucleotide reveals how this enzyme interacts with

62 wed no alteration in the processivity or the dideoxynucleotide sensitivity patterns.

63 of these SNPs were confirmed using both DNA dideoxynucleotide sequencing and the VDA methodologies.

64 Dideoxynucleotide sequencing of the conserved regions of

65 0 species) was examined by both conventional dideoxynucleotide sequencing of the rpoB and 16S genes a

66 r sample detected by conventional direct-PCR dideoxynucleotide sequencing.

67 fected individuals in two laboratories using dideoxynucleotide sequencing.

68 nucleotide primer extension and quantitative dideoxynucleotide sequencing.

69 be achieved with the fluorescent dye-labeled dideoxynucleotide system.

70 measurements we infer that selection against dideoxynucleotides takes place in the transition state f

71 g method that is a hybrid between the Sanger dideoxynucleotide terminating reaction and SBS.

72 stribution of the extension products and the dideoxynucleotide termination pattern suggest that nucle

73 Standard Sanger dideoxynucleotide termination performed in a large-volum

74 of DNA polymerases than regular dye-labeled dideoxynucleotide terminators or indeed normal dideoxynu

75 reactions are supplemented with azido-2',3'-dideoxynucleotides that randomly terminate DNA synthesis

76 als with solid-phase-capturable biotinylated dideoxynucleotides to generate Sanger DNA sequencing fra

77 bility of using solid phase capturable (SPC) dideoxynucleotides to generate single base extension (SB

78 are combined with four cleavable fluorescent dideoxynucleotides to perform SBS.

79 primers using a fluorescently labeled 2',3'-dideoxynucleotide triphosphate terminator was originally

80 ly biased against the incorporation of 2',3'-dideoxynucleotide triphosphates (ddNTPs) indicating very

81 by a single base in the presence of all four dideoxynucleotide triphosphates and a thermostable DNA p

82 ecific for blocking DNA polymerase alpha and dideoxynucleotide triphosphates at concentrations specif

83 The use of dideoxynucleotide triphosphates labeled with different f

84 ng the 3' ends of transforming DNA with 2'3' dideoxynucleotides, we have reduced the frequency of end