ライフサイエンスコーパス: diethyl pyrocarbonate

1 80% acetone or 100% acetone containing 0.1% diethyl pyrocarbonate.

2 so blocked by the histidine-reactive reagent diethyl pyrocarbonate.

3 ions inactivate LpxE, as does treatment with diethyl pyrocarbonate.

4 vity to osmium tetroxide, hydroxylamine, and diethyl pyrocarbonate.

5 by the histidine-selective acylating reagent diethyl pyrocarbonate.

6 ily reacting with the group specific reagent diethyl pyrocarbonate.

7 sidual activity was relatively unaffected by diethyl pyrocarbonate.

8 d mutagenesis and chemical modification with diethyl pyrocarbonate.

9 1 channel with the histidine modifying agent diethyl pyrocarbonate also enhanced the hSlo1 currents a

10 the internal pH sensitivity, suggesting that diethyl pyrocarbonate and low pH may work on the same ef

11 y due to inhibitory effects to the enzyme by diethyl pyrocarbonate and N-ethylmaleimide, respectively

12 lity of histidyl residues to modification by diethyl pyrocarbonate and observed that more than 50% pr

13 Last, studies with diethyl pyrocarbonate and p-chloromercuribenzenesulfonat

14 etal ions, and buffer conditions using OsO4, diethyl pyrocarbonate, and chloroacetaldehyde probes.

15 e was extremely susceptible to inhibition by diethyl pyrocarbonate, and protection against inhibition

16 ss spectrometry of the peptide modified with diethyl pyrocarbonate before and after Cu binding sugges

17 Modification of His residues with diethyl pyrocarbonate completely inhibited Zn2+ binding

18 were no longer sensitive to inactivation by diethyl pyrocarbonate, demonstrating that this is the in

19 Each of the five mutants is inactivated by diethyl pyrocarbonate (DEP), and inactivation is reversi

20 Given the rather low specificity of diethyl pyrocarbonate (DEPC) for histidine modification,

21 ment with the reversible histidine-modifying diethyl pyrocarbonate (DEPC) inhibited acyltransferase a

22 Treatment with diethyl pyrocarbonate (DEPC) inhibits reduction of the c

23 Diethyl pyrocarbonate (DEPC) modification of isoform E n

24 radical protection, circular dichroism, and diethyl pyrocarbonate (DEPC) modification.

25 thermophilus Rieske protein was reacted with diethyl pyrocarbonate (DEPC) over a range of pH values.

26 Diethyl pyrocarbonate (DEPC), a histidine residue-specif

27 s more pronounced with hydrophobic reagents [diethyl pyrocarbonate (DEPC), p-bromophenacyl bromide] a

28 e (DAH 7-P) synthase (Phe) is inactivated by diethyl pyrocarbonate (DEPC).

29 inorganic phosphate (P(i)) is inactivated by diethyl pyrocarbonate (DEPC).

30 e presence of reversible histidine-modifying diethyl pyrocarbonate (DEPC); 0.3 mM DEPC results in 95%

31 utaryl-CoA (HMG-CoA) lyase is inactivated by diethyl pyrocarbonate (DEPC); activity can be fully rest

32 Diethyl pyrocarbonate (DEPC; 10 mM) treatment of P2X4-in

33 Treatment with diethyl pyrocarbonate inactivated recombinant human QC w

34 on of HGPRT with either tetranitromethane or diethyl pyrocarbonate inactivated the enzyme completely,

35 NEM and diethyl pyrocarbonate inhibition suggested that the cata

36 Diethyl pyrocarbonate inhibits the reaction of cytochrom

37 bility shift assays and dimethyl sulfate and diethyl pyrocarbonate interference footprinting.

38 of EDTA or upon treatment of the enzyme with diethyl pyrocarbonate, it is proposed that Cu(2+) of L2

39 Through a series of diethyl pyrocarbonate modification experiments, it was f

40 Diethyl pyrocarbonate modification of His-4 and His-8 in

41 Diethyl pyrocarbonate modification, UV-melting profiles,

42 determined by native gel electrophoresis and diethyl pyrocarbonate modification.

43 etioplasts with 100% acetone containing 0.1% diethyl pyrocarbonate prevents the conversion of Pchlide

44 istidine-selective inactivation studies with diethyl pyrocarbonate provide further evidence regarding

45 inines and histidines with phenylglyoxal and diethyl pyrocarbonate, respectively, renders the enzyme

46 Cys and His residues using iodoacetamide and diethyl pyrocarbonate, respectively.

47 Treatment of the dehalogenase with diethyl pyrocarbonate resulted in complete loss of catal

48 The histidine-modifying reagent, diethyl pyrocarbonate, reversibly blocks cx50 hemichanne

49 ous biochemical investigations showed that a diethyl pyrocarbonate-sensitive histidine residue is at

50 Modification of histidine residues by diethyl pyrocarbonate specifically inhibited Zn(2+) bind

51 Inhibition is observed in the presence of diethyl pyrocarbonate, suggesting that a histidine is cr

52 n the protection of His from modification by diethyl pyrocarbonate when this residue binds Cu(II) in

53 that Lit was susceptible to inactivation by diethyl pyrocarbonate, with about three histidines rever