ライフサイエンスコーパス: digest

1 th peptide standards and a HeLa cell tryptic digest.

2 eparation (LC) for the analysis of a protein digest.

3 ptides was optimised using trypsin and SMART Digest.

4 ase HPLC mass spectrometry in tryptic plasma digests.

5 enriching phosphopeptides from yeast protein digests.

6 es and for peptide quantification in protein digests.

7 ch as casein alpha-S1, in whole meat product digests.

8 on for proteomic analysis of complex protein digests.

9 ribed, were also identified in the simulated digests.

10 lving power of 120000 in analysis of protein digests.

11 376 glycated peptides from 10 mug of protein digests.

12 ugs, a peptide standard mixture, and protein digests.

13 absence of Na(+), the N terminus was rapidly digested.

14 eriods of high prey availability to be later digested.

15 ntains nearly intact antibody while HCPs are digested.

16 aration of chromatin by micrococcal nuclease digest; 4) ChIP for open chromatin-associated histone me

17 Using this method, it was possible to digest 500mg of animal feed in a microwave system under

18 Carboxypeptidase N immediately digested 6 amino acids from the C terminus of the longer

19 C1 containing about 209 mg of collagenase to digest a 100-g pancreas).

20 enzyme mixture (NCEM) dose commonly used to digest a 100-g pancreas.

21 i-reactor gastrointestinal model was used to digest a mixture of pure polyphenol compounds, including

22 amyl moieties, and both enzymes were able to digest a small, synthetic tetrasaccharide dipeptide PG f

23 ficant adaptation to recognize, detoxify and digest a variety of host-plants.

24 because they are activated in the attempt to digest Abeta which starts a neuroinflammatory response t

25 ure fermentations with faecal inoculate, the digested acai pulp precipitated reductions in the number

26 ns from dairy farms, caused by anaerobically digesting (AD) cattle manure.

27 Human jejunal digests after oral ingestion of casein and whey protein

28 hydroxide and nitric acid in combination to digest all organic material within 1 h plus an additiona

29 emical approaches to determine if lambda-exo digests all parental DNA sequences equally.

30 hat it cleaves preferentially the hardest to digest amino acid, proline.

31 Whole digest and fractions>10, 5-10, and<5kDa caused cytotoxic

32 identification of phosphopeptides from serum digest and HeLa cell extract.

33 se bacterial communities, which collectively digest and recycle essential nutrients.

34 pigenetic assays, 5hmC sensitive restriction digest and ten-eleven translocation-assisted bisulfite p

35 So, raw and cooked samples were digested and afterward the elements calcium, magnesium,

36 Isolated proteins were digested and analysed with label-free two-dimensional li

37 peptide conjugating luciferase and Au NPs is digested and cut off, which results in the recovery of b

38 The inhibitory activity of the non-digested and digested samples towards metabolic syndrome

39 nanoemulsions (100 MPa) were partially (66%) digested and highly bioaccessible (>70%).

40 ulin (BLG), the main whey protein, is poorly digested and is highly allergenic.

41 rtens the time required for the sample to be digested and loaded to a resin to up to 2 h, and it was

42 Human serum and IGNIS prime peptides were digested and the IGNIS assay was used to quantify APO-F

43 Kidneys were digested and the phenotypes of renal leukocyte populatio

44 inal fluidity while reclaiming water used in digesting and absorbing a meal, and is closely regulated

45 he Venus flytrap effectively detects, traps, digests and absorbs insect prey.

46 ers that were evaluated for binding to tumor digests and cultured TILs used for the treatment of pati

47 scular disease until 12 months, when trypsin digests and dye leakage assays showed high fat-fed mice

48 t number of peptides identified in beef meat digests and provides a comprehensive database for meat p

49 EM, SEM, and total metals analysis of fabric digests and wash water filtrates.

50 des were identified from 50 ng of an E. coli digest, and 2158 protein groups and 10005 peptides were

51 leanup by immunoaffinity extraction, tryptic digest, and preconcentration by solid-phase extraction (

52 hydrolysis was carried out in each protease digest, and the higher degree of hydrolysis was observed

53 copeptides, obtained via controlled protease digest, and their characterization by using advanced mas

54 ed species was recovered from the monolayer, digested, and deuterium incorporation monitored by MS.

55 , based on the analysis of intact, partially digested, and digested/reduced mAb samples.

56 ant human clusterin at a 1:1 molar ratio and digested, and the actual ratios for each combination of

57 A pool of preterm human milk was digested as raw or after Holder pasteurization.

58 tion (ChIP) and micrococcal nuclease (MNase) digest assays were performed to examine the changes in c

59 than in the smooth porridge (33% less starch digested at 90 min, P < 0.05, paired t test).

60 eriments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectro

61 nature of the original pretreated and enzyme digested biomass.

62 ments performed in rabbits demonstrated that digested boiled shrimp extract is able to induce IgG ant

63 simple and efficient way; LC-MS of a trypsin-digested bovine serum albumin (BSA) sample provided narr

64 the direct analysis of peptides from trypsin digested bovine serum albumin.

65 ration was at 5 ng/mul, LHRHa was completely digested by 5h of incubation, or earlier.

66 Samples were digested by a sialate-O-acetylesterase (NanS) to confirm

67 romatin is a potential self-antigen normally digested by circulating DNASE1L3.

68 The food samples were digested by dry ashing procedure and their minerals were

69 ivated platelets adhered were and ultimately digested by ECs in vivo and in vitro.

70 in-alpha5 in the islet BM were significantly digested by enzyme treatment.

71 proteins from buffalo colostrum & milk were digested by in vitro simulation digestion and analyzed b

72 y which dead or dying cells are engulfed and digested by phagocytes.

73 others (e.g. CYR61 and tenascin-C) could be digested by several KLKs.

74 he HRMS analysis, the remaining samples were digested by trypsin, followed by SRM detection.

75 the gut by bacterial fermentation of poorly digested carbohydrates.

76 secretion, consistent with their ability to digest carcasses.

77 system works for complex mixtures of trypsin-digested cell lysate.

78 FESEM imaging of xyloglucanase-digested cell walls revealed an altered microfibril orga

79 nalise components of host blood by endocytic digest cells that line the tick midgut epithelium.

80 omes to block Exo1, yet Exo1 also appears to digest chromatin with high processivity and at rates sim

81 es the dependence on a restriction enzyme to digest chromatin, instead relying on the endonuclease DN

82 cleosome-sized (such as 101-140 bp) peaks of digested chromatin fragments with MPE-seq but not with M

83 DNASE1L3 is uniquely capable of digesting chromatin in microparticles released from apop

84 urrent nucleosome-mapping strategies involve digesting chromatin with nucleases or chemical cleavage

85 from goat milk tended to be more efficiently digested compared to caseins from cow milk and peptide p

86 s, the results indicated that LHRHa would be digested completely in the human stomach and would pose

87 Wild type and acid tolerant strains digested corn stover with equal efficiency in batch cult

88 raw organic wastes (predigestion) and their digested counterparts (postdigestion, i.e., digestates).

89 scar with chondroitinase ABC (ChABC), which digests CSPG, would further allow caRheb-transduced neur

90 ile chondroitinase ABC (ChABC) enzymatically digests CSPGs, which are upregulated at the site of inju

91 D method was demonstrated by using a mask to digest designated sections of the sample surface with th

92 A mutualistic role of gut microbes is to digest dietary complex carbohydrates, liberating host-ab

93 ELAs) are pancreatic serine proteinases that digest dietary proteins.

94 tion, were used to determine if these cherry digests differed in their ability to protect Caco-2 cell

95 d to yield pure hybrids free of polydisperse digested DNA fragments and serum biomolecules.

96 pproach that combines BAL31 digestion, which digests DNA from the ends and chromosome breaks, with ne

97 DNase II digests DNA in endolysosomes.

98 TREX1 is an exonuclease that digests DNA in the cytoplasm.

99 ice lacking DNaseII, a lysosomal enzyme that digests DNA, rescued the lethal autoimmune phenotypes of

100 Here, we digest Drosophila chromatin with high and low concentrat

101 isulfide bonding from nonreduced proteolytic digests, due to the concurrent detection of disulfide-co

102 in contrast, proteins that are more readily digested (e.g., SurA) serve as more sensitive reporters

103 Moreover, the colon-digested EDB extract inhibited oxidant-induced mutagenic

104 SMART Digest enabled complete digestion in 30 minutes compared

105 s that chromatin be chemically cross-linked, digested, end-repaired, and proximity-ligated with a bio

106 Low-dose RCEM was efficient in digesting entire pancreases to obtain higher yield (5535

107 and an unlabeled yeast strain were mixed and digested enzymatically in solution.

108 topes and treatment of tissue with cell wall digesting enzymes, coupled with bioassay of guard cell f

109 missed cleavage peptides in protein tryptic digests even at prolonged digestion times.

110 n effectively separate peptides from protein digests exist, very few MDLC strategies, primarily consi

111 unds due to the digestion process, the colon-digested extract was able to reduce the excessive intrac

112 rich DNA, such that A/T-rich nucleosomes are digested faster than G/C-rich nucleosomes.

113 BACKGROUND & AIMS: Poorly digested, fermentable carbohydrates may induce symptoms

114 Poorly digested, fermentable carbohydrates may induce symptoms

115 Malabsorption of partially digested food causes bloating, overfilled intestines, ab

116 d was applied to various water and microwave digested food samples.

117 successfully applied to real water and acid digested food samples.

118 an 10-fold the radical scavenging ability of digested fractions compared with undigested seasonings.

119 Digested fractions derived from the seedless wine pomace

120 e reducing and antiradical capacities of the digested fractions.

121 This strategy ensures that only double-digested fragments are amplified and sequenced.

122 Cells treated with digests from cherries identified as containing high anth

123 report that lambda-exo does not efficiently digest G-quadruplex (G4) structures in a plasmid.

124 e used chronoamperometry to measure protease-digested GA samples.

125 tions rely on restriction enzymes capable of digesting genomic DNA at defined sequence motifs.

126 s include isolating and lysing single cells, digesting genomic DNA with a methylation-sensitive restr

127 imordial germ cells by actively excising and digesting germ cell lobes.

128 represents a novel approach to quantify the digested gluten peptides in human urine with outstanding

129 Digesting glycosaminoglycan side chains of CSPG with cho

130 arana, using Caco-2 cells, and the effect of digested guarana on carbohydrate metabolism enzymes.

131 HYAL2), one of two major hyaluronidases that digest HA in somatic tissues.

132 lity of SNM1A to accommodate and efficiently digest highly distorted DNA substrates, such as those co

133 ribonuclease that has the unusual ability to digest highly structured RNA.

134 3' to 5' hydrolytic exoribonuclease able to digest highly structured RNA.

135 tion and sensitivity to separate mixtures of digested HS oligosaccharides.

136 elected for subsequent identification in the digests/hydrolysates.

137 t peptides from a simulated gastrointestinal digest (Id) from Amaranthus mantegazzianus proteins (I),

138 ay, we simulate HIV-1 maturation in vitro by digesting immature particles and assembled virus-like pa

139 1 peptides were identified from 50 ng of the digest in a 2.5 h analysis, which approaches the identif

140 e dried at 100 degrees C for 2 days and then digested in a mixture of nitric acid/hydrogen peroxide (

141 icrobial populations in the feed sludge were digested in comparison with less than 50% calculated usi

142 ant, i.e. Prunin 1 and 2, were enzymatically digested in silico predicting 10 and 14 peptides, respec

143 The soluble kiwifruit fibre was highly digested in the foregut (80%), and the insoluble fibre i

144 red as a source of dietary fiber that is not digested in the mammalian digestive system.

145 io (P = 0.002) All 5 RS-containing bars were digested in vitro approximately 30% more slowly than the

146 Spinach (4g) was digested in vitro with added magnesium (0, 200, 400mg/L)

147 r of proteomics, where discrete spots can be digested in-gel, and proteins subsequently identified by

148 e undigested porridges and simulated gastric digests inhibited LDL oxidation.

149 embly of amyloid-like fibrils from partially digested insulin fragments.

150 unoprecipitated, rigorously washed, and then digested into peptides while avoiding antibody contamina

151 The effect of digested juice on Caco-2 cells proliferation was also st

152 but they are still lower than in initial non-digested juice.

153 tose intolerance is the decreased ability to digest lactose, and the population involved is rapidly i

154 acterium Escherichia coli makes proteins for digesting lactose only when lactose is available and glu

155 eversed-phase HPLC for purity (99%), peptide digest LC-MS/MS for sequence determination, and circular

156 Allometric constraints associated with digesting leaves require relatively small avian herbivor

157 e phenotyped by multicolor flow cytometry in digested lung tissue and bronchoalveolar lavage (BAL) si

158 Starting with cell encapsulation in digested lymphoid tissues, clusters of proliferating B c

159 ans, an algae-associated flavobacterium that digests many polysaccharides, including alginate.

160 ried out with untreated (control) and enzyme-digested matrices.

161 ies based on this strategy have consistently digested milligram amounts of protein as starting materi

162 The disappearance of epitopes in the digested mixtures could explain the lower IgE binding ob

163 a stable active MNAzyme, which continued to digest multiple hairpin H0 substrates, concomitantly gen

164 MMP-7 specifically digests negatively charged JR2EC immobilized on rGO, the

165 droxy-2-nonenal concentrations compared with digested nitrite-cured raw and cooked pork.

166 With only 100 mug of tryptic digested, nonstimulated HeLa protein and 45 h of LC-MS a

167 Analyzing digested normal human skin by single-cell RNA sequencing

168 n of the processes used by microorganisms to digest nutrients for their growth can be a viable method

169 uding the effects of gut microbiota over non-digested nutrients.

170 LC/MS data from a protein digest obtained at an independent laboratory demonstrate

171 Application of the method to a tryptic digest of bovine coagulation factor V resulted in identi

172 of 2D FT-ICR MS are explored with a tryptic digest of cytochrome c with both ECD and IRMPD as fragme

173 me transglutaminase 2) or the peptic-tryptic digest of gliadin (in native and deamidated forms) befor

174 pecific targets of the hmAb 1002-1E03 from a digest of gliadin treated by transglutaminase 2.

175 The input (starting peptide digest of prolamins), the flow-through (unbound peptides

176 versed-phase C18 functionality) to a tryptic digest of whole Jurkat cell lysate to estimate the depth

177 sequence coverage for both trypsin and Lys C digests of bovine serum albumin, providing ECD spectra f

178 ride isolated from bacterial heparin lyase 1 digests of heparin that contains a Delta-2S-iduronate on

179 ing, and annotating Cys34 adducts in tryptic digests of human serum/plasma.

180 bility by combining information from several digests of increasing depths.

181 Simulated duodenal digests of the porridges had hydroperoxides and therefor

182 release was compared with that found in the digests of the same substrates using a standardised prot

183 -linked precursors, derived from the tryptic digests of three model proteins (Human Serum Albumin, cr

184 to consume beyond what is sustainable (i.e. digested on a daily basis), but this phenomenon of hyper

185 By 24h of incubation, LHRHa was completely digested or barely detectable if the starting material w

186 hat has evolved to invade, reseal, kill, and digest other gram-negative bacteria in soils and water e

187 mbda exonuclease (lambda-exo) to efficiently digest parental DNA while leaving RNA-primer protected n

188 from the XPF-ERCC1-RPA-induced incisions and digest past the crosslink to quantitatively complete the

189 ls, present in all tissues, which engulf and digest pathogens, infected and dying cells, and debris,

190 Digested PC extract exhibited intracellular ROS-inhibito

191 ge due to colon mucosa cells' treatment with digested PC extract was observed.

192 rimental mass spectra with the theoretically digested peptides derived from a reference protein datab

193 Unlike T cells that recognize digested peptides, B cells recognize their cognate antig

194 inent gut symbiont Bacteroides ovatus, which digests polysaccharide at a cost to itself but at a bene

195 are unique among carnivorous plants as they digest prey in a complex mutualistic association in whic

196 alysis of a Chromeo P503-labeled BSA tryptic digest produced a 2D separation that made effective use

197 ectral features of PTM-dependent proteolytic digest products promise a generalized PTM site-mapping s

198 The digested products are transported out of the lysosome vi

199 tion of plasmepsin 2-3, encoding haemoglobin-digesting proteases, regardless of the location.

200 tion of plasmepsin 2-3, encoding haemoglobin-digesting proteases, regardless of the location.

201 In the initial stage, markers from tryptic digested protein of chilled, boiled and autoclaved pork

202 d of DS(-) from small volumes of peptide and digested protein samples prior to ESI-MS.

203 Higher % peptide recoveries from digested protein samples were obtained using the propose

204 Trypsin-digested proteins from Saccharomyces cerevisiae were tag

205 -modified membrane at the end of a pipet tip digests proteins in 30 s or less, and the short proteoly

206 he relevant antioxidant capacity revealed by digested puffed kernels can be ascribed to both the new

207 The HMW fractions were IdeS digested, reduced, and analyzed by size-exclusion chroma

208 analysis of intact, partially digested, and digested/reduced mAb samples.

209 through their digestive tract only partially digested, releasing endosymbiotic algae that still suppo

210 nd fermentation of the pretreated and enzyme-digested residues surprisingly resulted in ethanol yield

211 Using double-digest restriction-site associated DNA sequencing, we su

212 central, and eastern) via analysis of double digest restriction-site associated DNA using Next Genera

213 ample preparation technique for difficult-to-digest samples containing high carbon content, especiall

214 concentrations were measured in nitric acid-digested samples and haem Fe was extracted using acidifi

215 ile acid (BA)-binding capacities of in vitro digested samples and nutritionally important products pr

216 sensor strips were able to measure protease-digested samples containing GA in very small sample volu

217 inhibitory activity of the non-digested and digested samples towards metabolic syndrome-associated e

218 higher in the dry-ashed samples than the wet-digested samples.

219 Trypsin-digested, SDA-cross-linked human serum albumin (HSA) ser

220 orphisms obtained via restriction-associated digest sequencing.

221 ssessed the concentrations of 69 elements in digested sewage sludge and effluent samples from 64 muni

222 Chymotrypsin was used to digest sheep recombinant PrP to identify a set of charac

223 In vivo and in vitro digests showed comparable peptide profiles and high numb

224 dentified in only 10 mug of membrane protein digests, showing the superior sensitivity of the Hp-RP S

225 ne-bound beta-glucosidase could specifically digest soluble xyloglucan without affecting the wall-bou

226 The digested solution was injected into the FIC system, and

227 ion of total selenium in water and microwave digested some food samples with quantitative recoveries

228 its ability to efficiently and reproducibly digest specific classes of gluten in barley.

229 gh starch intake but low genetic capacity to digest starch had the lowest BMI, potentially because la

230 asting temperature, peak viscosity, and slow digesting starch (from 100 to 274mg/g) in normal, but no

231 MY2 genes, respectively) are responsible for digesting starchy foods.

232 t layer of cereal endosperm and functions to digest storage products accumulated in starchy endosperm

233 ound, amounting to nearly 12 mg per 100 g of digested strawberries.

234 p a detailed model that explains how RNase R digests structured RNA and how this differs from its act

235 lated by HPLC fractionation of enzymatically digested subunits.

236 e bioactive extracts, liposomes and in vitro digested surimi gels were determined.

237 molecules and a bovine serum albumin tryptic digest, TASF improved the peak shape and resolution in c

238 It seemed to be less digested than dicoccum and durum wheat pasta.

239 Tropomyosin in shrimp extract is worse digested than purified tropomyosin.

240 Macrophages engulfed and digested that speck-containing, pyroptotic debris.

241 cidental biological uptake can enzymatically digest the sugar based core.

242 NP to a pilot wastewater treatment plant and digested the sludge anaerobically.

243 o III recycles the target DNA by selectively digesting the dye-tagged sequence-matched probe DNA stra

244 CFTR peptides produced from proteolytically digesting the plasma membrane subproteome of cells.

245 reated milk, and infant formulas (IFs) after digesting the precipitated proteins with trypsin.

246 tion of Exo I as an enzyme which selectively digests the 3'-end of single stranded DNA (ssDNA).

247 Micrococcal nuclease (MNase) digests the linker to yield the chromatosome, containing

248 ent in both the nucleus and mitochondria and digests the RNA component of RNA-DNA hybrids.

249 the presence of RNase H, which specifically digests the RNA strands within RNA-DNA hybrids.

250 pproach of analyzing proteins by LC-MS is to digest them into peptides, which can serve as surrogates

251 ly biting deeply into bones, pulverizing and digesting them.

252 Although this organism digests these polysaccharides extracellularly, mutants l

253 Starting with the plating of digested tissue material, full-grown organoids can usual

254 fixed by coronary perfusion or enzymatically digested to isolate ventricular myocytes, which were sub

255 s isolated from the raw and cooked beans and digested to simulate human digestion.

256 matographic (LC) separation of optimized RNA digests to greatly simplify the interpretation of comple

257 We investigated its ability to digest triglycerides in a test meal and its efficacy in

258 beta-lactoglobulin was poorly digested under all gastric digestion conditions.

259 uits enriched in proteins and/or fibres were digested under the same conditions.

260 cleosome-free but instead occupied by easily digested, unstable, "fragile" nucleosomes.

261 Upon nutrient starvation, autophagy digests unwanted cellular components to generate catabol

262 ptamer) and a DNA binding dye, berberine, is digested upon the addition of exonuclease I, leading to

263 eptides were identified from 25 ng of a HeLa digest using single-shot analysis with a SCARAFT-acrylam

264 gg powder and its fractions were efficiently digested using 50mmolL(-1)NH4OH as an absorbing solution

265 Biscuits were digested using a multi-compartmental and dynamic system

266 ible of trace elements and heavy metals were digested using closed-nitric acid digestion and Rijksins

267 es C for one day, ground thoroughly and then digested using nitric acid/hydrogen per oxide (3:1).

268 was investigated using diluted anaerobically digested vinasse as cultivation medium.

269 and amount of antioxidants in undigested or digested WB.

270 Across peptide standards and protein digests, we demonstrate selective infrared multiphoton d

271 eptides followed by mass spectrometry of the digests, we demonstrated that the mutant sequence is sev

272 solved, and peptides from an albumin tryptic digest were much better resolved than with existing comm

273 alkylation, and digestion with trypsin, the digests were eluted with 200 mM ammonium bicarbonate at

274 Tryptic digests were prepared in 50 mM formic acid and loaded on

275 Digests were then loaded onto an online peptide immuno-a

276 , glycophorin A, ovalbumin and gp120 tryptic digests were used to build a spectral database of N- and

277 barrier remains intact, but that is rapidly digested when cells are lysed before evaluation.

278 ein phosphopeptides from a simulated tryptic digest with bovine serum albumin (BSA:casein, 100:1).

279 d mainly by bile concentration, with samples digested with 1mM of bile being more susceptible to inhi

280 o inhibitory effects of magnesium than those digested with 8mM (p<0.001).

281 Biopsies were incubated with gluten digested with chymotrypsin (modified or unmodified by th

282 Nase-seq libraries, cells are lysed and then digested with DNase I.

283 soluble pectins from strawberry fruits were digested with endo-polygalacturonase M2 from Aspergillus

284 All proteins were digested with endolysosomal extracts, and the resulting

285 The PCR products (294 bp) was subsequently digested with HinfI (New England Biolabs) at 37 degrees

286 In addition, the same extract was digested with LysN to confirm or further narrow down the

287 The acid denatured protein was digested with pepsin and analyzed by liquid chromatograp

288 y of serum sickness, the equine antisera was digested with pepsin to generate F(ab')2 fragments, with

289 ssues and 14- to 26-fold relative to samples digested with RNAse R to selectively remove linear RNA.

290 Plasma samples were digested with trypsin and analyzed with liquid chromatog

291 Few microliters of milk samples have been digested with trypsin and chymotrypsin and analyzed by n

292 series of buffers, the membrane proteins are digested with trypsin and quantitated by parallel reacti

293 The enriched fractions were digested with trypsin and RIP-II peptides were identifie

294 Simultaneously, the protein is digested with trypsin and the resulting fragments are pu

295 roduce both 100 bp and 1 kb DNA ladders when digested with two common restriction enzymes.

296 articles were efficiently separated from the digests with a hand-held magnet prior to analysis.

297 This condition allowed final digests with residual acidity and residual carbon conten

298 in which parts of eukaryotic cells are self-digested within the lysosome or vacuole.

299 ulsions containing small droplets were fully digested within the small intestine phase, and led to a

300 s, resulted in the accumulation of partially digested xyloglucan subunits, such as GXXG, GXLG, or GXF