ライフサイエンスコーパス: digitonin

1 entrifugation and membrane solubilization by digitonin.

2 assayed in situ after permeabilization with digitonin.

3 ved after permeabilization of the cells with digitonin.

4 abilization of membrane proteins by CHAPS or digitonin.

5 embranes were selectively permeabilized with digitonin.

6 nophore combinations (nigericin and CCCP) or digitonin.

7 orescence microscopy and catalase release by digitonin.

8 DNA synthesis induced by another detergent, digitonin.

9 ry alphabeta T cells after solubilization in digitonin.

10 in B or nonselectively to small solutes with digitonin.

11 eased from infected cells permeabilized with digitonin.

12 and SDS-lysed cells or from cells lysed with digitonin.

13 assayed in situ after permeabilization with digitonin.

14 ed hypersensitivity of the outer membrane to digitonin.

15 epithelial cells (HLE-B3) permeabilized with digitonin.

16 ibroblasts that have been permeabilized with digitonin.

17 -3 by surface membrane permeabilization with digitonin.

18 After permeabilization with digitonin, 50% of total glucokinase remained bound intra

19 dysenteriae with different concentrations of digitonin, a steroid glycoside that interacts with chole

20 On-column treatment with digitonin allowed for the separation of the plasma membr

21 ormational change is blocked in solutions of digitonin, although changes in optical absorbance accomp

22 complex by using the nondenaturing detergent digitonin and a one-step immunoaffinity technique.

23 lasma membrane permeabilizing agents such as digitonin and acetone/methanol, while cholesterol deplet

24 mbrane-permeable cholesterol-binding agents (digitonin and nystatin), but not the lipid-binding agent

25 the exported complexes by being stable in 1% digitonin and unstable in 1% Nonidet P-40.

26 face in solutions of dodecyl maltoside (DM), digitonin, and phospholipid bilayers of two compositions

27 The nonionic detergent, digitonin, and the anionic detergent, sodium dodecyl sul

28 s in state II, whose binding is sensitive to digitonin, and which are absent in DeltaLhca4.

29 Treating cells with digitonin at 5 micrograms/ml permeabilizes the plasma me

30 -treated 143B.TK(-) cells permeabilized with digitonin at a concentration not affecting the mitochond

31 ddress the intracellular functions of p22, a digitonin-based "bulk microinjection" assay was develope

32 Two of these compounds, amiodarone and digitonin, can act synergistically with all-trans-retina

33 rin from Bacillus subtilis QST713 as well as digitonin, CHAPS, and lysophosphatidylcholine solubilize

34 The sterol-containing agent digitonin completely inactivated IPC synthase.

35 Cells are permeabilized using saponin (SAP), digitonin (DIG) or recombinant perfringolysin O (rPFO) (

36 el electrophoresis in the presence of 0.003% digitonin displayed a supercomplex composed of homodimer

37 ria that may have been also permeabilized by digitonin do not contribute to ATP consumption by the ex

38 DTrc8 expression increased the level of digitonin-extractable CSN complex, consistent with eleva

39 evidence for specific binding of cytoplasmic digitonin-extractable glucokinase.

40 er photolysis measurements were conducted on digitonin extracts of artificial pigments prepared from

41 Digitonin extracts of mitochondria from cardiolipin-cont

42 es of similar composition were identified in digitonin extracts of ribosome-free membranes, indicatin

43 Using digitonin fractionation and fluorescence microscopy of F

44 Mis-localisation was confirmed by digitonin fractionation experiments.

45 p proteins by tyeA mutant yersiniae with the digitonin fractionation technique.

46 Using immunoblotting, digitonin fractionation, immunofluorescence, and electro

47 hicken stomach membranes were solubilized in digitonin, glycoproteins were separated from solubilized

48 ells, indicated that the cells responding to digitonin had already been primed for apoptosis, and tha

49 hat purified active human gamma-secretase in digitonin has a total molecular mass of approximately 23

50 permeabilization of the plasma membrane with digitonin; (ii) insertion of Factor Xa cleavage sites; (

51 Use of the mild detergent digitonin in immunofluorescence also allowed centrin 2 t

52 es of complexes solubilized in the detergent digitonin, in which bivalency was not observed.

53 s retained after treatment of the cells with digitonin, indicating that it may interact with a membra

54 n, the majority of the transfected beta1D is digitonin-insoluble and is strongly associated with the

55 lack of membrane association and revealed a digitonin-insoluble nuclear fraction of S100A2, which wa

56 ither 10 microM amphotericin B or 10 micro M digitonin led to an aqueous-to-serosa-positive ISC.

57 n the plasma membrane was permeabilized with digitonin or in membrane preparations but not in intact

58 e permeabilization of transfected cells with digitonin or Triton X-100, respectively.

59 ere permeabilized without detergents such as digitonin or Triton X-100.

60 pt the mitochondrial outer membrane, such as digitonin, or maintain outer membrane exchange of adenin

61 estruction of PP or PV sites was achieved by digitonin perfusion into the portal or inferior vena cav

62 After digitonin permeabilization of these cells, we found that

63 Cells depleted of ARF and Rho by digitonin permeabilization showed a significant reductio

64 nt study, submitochondrial fractionation and digitonin permeabilization studies indicated that both c

65 ods (detergent lysis, Dounce homogenization, digitonin permeabilization, and sonication) and three pr

66 s abolished Ca2+ -evoked secretion following digitonin permeabilization, compared with partial inhibi

67 ined to be exclusively within peroxisomes by digitonin permeabilization, immunofluorescence, protease

68 Interestingly, we found that digitonin permeabilization, which selectively releases s

69 Cytosolic calnuc was released by mild digitonin permeabilization.

70 Ran, which is released from cells during the digitonin permeabilization.

71 Digitonin-permeabilization studies of SINC-GFP-transfect

72 glucokinase overexpression combined with the digitonin-permeabilization technique.

73 es not stimulate nuclear protein import in a digitonin permeabilized cell assay and also inhibits wil

74 , that exhibited unexpected behavior both in digitonin-permeabilized and microinjected mammalian cell

75 nylenediamine as substrate, oxygen uptake by digitonin-permeabilized apoptotic mitochondria was great

76 is nucleotide inhibited cGMP accumulation in digitonin-permeabilized Caco-2 human colon carcinoma cel

77 se peptides for nuclear import activity in a digitonin-permeabilized cell assay.

78 Using digitonin-permeabilized cell assays to measure nuclear i

79 In a digitonin-permeabilized cell docking assay, RanBP1 stabi

80 Using a digitonin-permeabilized cell in vitro nuclear import sys

81 By using the digitonin-permeabilized cell system for protein import i

82 e requirements for plasmid nuclear import, a digitonin-permeabilized cell system was adapted to follo

83 lasmic fibers of the nuclear pore complex in digitonin-permeabilized cells and RanBP1 competition con

84 ity to stimulate the nuclear export of GR in digitonin-permeabilized cells and that the inhibition is

85 nses elicited from populations of intact and digitonin-permeabilized cells as well as from cells unde

86 Pax6 by Kap13 was shown to occur by using a digitonin-permeabilized cells assay.

87 in malate/glutamate-dependent respiration in digitonin-permeabilized cells by approximately 90% and a

88 th the binding data, nuclear import of L2 in digitonin-permeabilized cells could be mediated by eithe

89 mbly assay using purified COPII proteins and digitonin-permeabilized cells has been applied to demons

90 Similarly, KCN titration assays on digitonin-permeabilized cells have revealed a COX capaci

91 r nuclear import on a non-nuclear protein in digitonin-permeabilized cells in a temperature- and ATP-

92 Nuclear import in digitonin-permeabilized cells in the absence of added fa

93 at the nuclear envelope (NE) was observed in digitonin-permeabilized cells in the absence of cytosoli

94 hovanadate (Na(3)VO(4)) to medium containing digitonin-permeabilized cells inhibits all ADP-ATP-using

95 wever, mitochondrial respiration measured in digitonin-permeabilized cells is impaired in unstable cl

96 Nuclear export of GFP-NFAT in digitonin-permeabilized cells occurs in a temperature- a

97 ies with RanGDP-conjugated colloidal gold in digitonin-permeabilized cells showed a large number of R

98 We have used digitonin-permeabilized cells to examine in vitro nuclea

99 uclear accumulation of the conjugated DNA in digitonin-permeabilized cells via the classical pathway

100 ous nuclear karyopherin alpha from nuclei of digitonin-permeabilized cells was quantitatively monitor

101 lucokinase and of phosphoglucoisomerase from digitonin-permeabilized cells was slower when cells were

102 import of HIV-1 pre-integration complexes in digitonin-permeabilized cells was used to demonstrate th

103 In digitonin-permeabilized cells, beta2 was able to dock A1

104 s reacted with the biotinylating reagents in digitonin-permeabilized cells, demonstrating that the ab

105 Using digitonin-permeabilized cells, it was shown that the tra

106 In digitonin-permeabilized cells, Kap beta2B mediates TAP-G

107 lassical nuclear localization signal, and in digitonin-permeabilized cells, nuclear accumulation of J

108 Addition of IP3 to digitonin-permeabilized cells, or agonist treatment of i

109 In digitonin-permeabilized cells, the Kap but not Crm1 stim

110 genase-dependent respiration, as measured in digitonin-permeabilized cells, was specifically decrease

111 Using digitonin-permeabilized cells, we show that SMN import d

112 The linking reaction was also shown in digitonin-permeabilized cells, with UDP-N-acetylglucosam

113 ion signal (NLS)-dependent protein import in digitonin-permeabilized cells.

114 TP) activity in isolated mitochondria and in digitonin-permeabilized cells.

115 oorly with [3H]myristate or [3H]stearate) in digitonin-permeabilized cells.

116 y was studied using pulse-chase labeling and digitonin-permeabilized cells.

117 ycocyanin (APC) in standard import assays in digitonin-permeabilized cells.

118 luorescence recovery after photobleaching in digitonin-permeabilized cells.

119 ersion and induced nuclear import of NEMO in digitonin-permeabilized cells.

120 adhesive and lateral dimers was observed in digitonin-permeabilized cells.

121 ort the import of pA-RCC1 into the nuclei of digitonin-permeabilized cells.

122 TF2 can stimulate the accumulation of Ran in digitonin-permeabilized cells.

123 nsulin receptor in a cell-free system and in digitonin-permeabilized Chinese hamster ovary (CHO) cell

124 e epidermal growth factor (EGF) receptors in digitonin-permeabilized CHO cells that overexpress human

125 % of the ATP-dependent secretion observed in digitonin-permeabilized chromaffin cells.

126 80 was efficiently imported in the nuclei of digitonin-permeabilized COS-7 cells in the presence of r

127 Analyses of cathepsin B and D activities in digitonin-permeabilized cultures and the monitoring of c

128 e been directly compared after expression in digitonin-permeabilized fibroblastic (CV-1) cells, provi

129 ochondrial Ca2+ uptake capacity using either digitonin-permeabilized GT1-7 neural cells or isolated G

130 ier to gene transfer, was undertaken using a digitonin-permeabilized HeLa cell assay.

131 was effectively imported into the nucleus of digitonin-permeabilized HeLa cells after incubation with

132 uclear import of the HPV16 E6 oncoprotein in digitonin-permeabilized HeLa cells could be mediated by

133 ed E1 protein failed to enter the nucleus of digitonin-permeabilized HeLa cells in vitro.

134 In vitro import assays in digitonin-permeabilized HeLa cells reveal that ORF29p is

135 When digitonin-permeabilized HeLa cells were incubated with H

136 streptavidin is imported into the nuclei of digitonin-permeabilized HeLa cells.

137 mic determinants of pDNA nuclear import into digitonin-permeabilized HeLa cells.

138 d the nuclear import pathways of HPV16 E6 in digitonin-permeabilized HeLa cells.

139 d nuclear uptake of fluorescent substrate in digitonin-permeabilized HeLa cells.

140 in the mitochondrial intermembrane space of digitonin-permeabilized hepatocytes, indicative of VDAC

141 tion assays of apolipoprotein B100 (apoB) in digitonin-permeabilized HepG2 cells indicated that multi

142 were concentrated in perinuclear regions of digitonin-permeabilized HepG2 cells.

143 We used digitonin-permeabilized human cells to analyze the mecha

144 tivity of anti-7A6 was observed in normal or digitonin-permeabilized human peripheral blood lymphocyt

145 dk2/cyclin E and Cdc2/cyclin B1 complexes in digitonin-permeabilized mammalian cells and also examine

146 02) inhibited NE uptake in intact as well as digitonin-permeabilized PC12 cells, but had no effect on

147 NE uptake into large dense core vesicles in digitonin-permeabilized PC12 cells, which indicates that

148 e-8 or caspase-3 stimulates OPA1 cleavage in digitonin-permeabilized rat brain mitochondria, suggesti

149 Furthermore, cell-free incubation of digitonin-permeabilized TC7 cells with the nido-OPDs res

150 In addition, a digitonin-permeabilized, cell-free transport in vitro as

151 t to restore agonist-induced PLD activity to digitonin-permeabilized, cytoplasm-depleted cells.

152 In addition, both intact and digitonin-permeablized COS-7 cells transfected with CD39

153 SOAT2-CHO cells prepared by a treatment with digitonin (plasma membrane permeabilized), BeauIII selec

154 subjected to saponification, extraction, and digitonin precipitation.

155 Treatment of mitochondria with digitonin resulted in a 2-fold higher release of cytochr

156 solubilization of rough microsomes (RM) with digitonin, ribosomes co-sedimented in complexes containi

157 Digitonin solubilization of mitochondria demonstrated th

158 Digitonin solubilization of thylakoid membranes releases

159 recipitated by immobilized streptavidin from digitonin-solubilized cells that had been treated with N

160 ontaining the RC-LH1 and -LH2 complexes from digitonin-solubilized chromatophores revealed high level

161 Separation of digitonin-solubilized mitochondrial extracts in one- and

162 biotinylated-Coq3 and Coq4 polypeptide from digitonin-solubilized mitochondrial extracts shows their

163 The digitonin-solubilized OT complex was catalytically activ

164 hown that Cu(2+)-phenanthroline treatment of digitonin-solubilized preparation provides the most effi

165 electrophoresis and coimmunoprecipitation of digitonin-solubilized thylakoids showed that Hcf106 and

166 crylamide gel electrophoresis (BN-PAGE) from digitonin-solubilized thylakoids were similar in the wil

167 osome does not define the composition of the digitonin-soluble translocon.

168 Whereas in digitonin solution a highly electrostatic ribosome-trans

169 otein-coupled receptor (RGR) was isolated in digitonin solution from bovine RPE microsomes and copuri

170 Association produces digitonin stable complexes with an estimated mass of 950

171 tochondria with increasing concentrations of digitonin suggested that the NADH dehydrogenase is loose

172 f cellular beta1A integrin is extractable in digitonin, the majority of the transfected beta1D is dig

173 different techniques: permeabilization with digitonin to allow extracellular ATP to equilibrate with

174 ation of the eukaryotic plasma membrane with digitonin to measure Yop targeting during Yersinia infec

175 yed in situ using very low concentrations of digitonin to render their plasma membrane permeable to s

176 use of the ability of low concentrations of digitonin to selectively permeabilize the plasma membran

177 croscopy of cells treated with the detergent digitonin to selectively permeabilize the plasma membran

178 Using a low concentration of digitonin to selectively permeabilize the plasma membran

179 Selective permeabilization of cells with digitonin (to permeabilize the plasma membrane) or Trito

180 In digitonin-treated cells, the electropherograms consisted

181 ration 1, 2, and 5 days posthepatectomy when digitonin-treated microsomes were used.

182 es, p-nitrophenol and estrone, in intact and digitonin-treated microsomes.

183 re, the striking observation that controlled digitonin treatment caused a massive and very rapid rele

184 d to this meshwork, being extracted by brief digitonin treatment.

185 and timed exposure to the pore-forming agent digitonin, we controlled the plasma membrane permeation

186 basal respiration was slightly increased by digitonin when the cells were incubated in medium contai