ライフサイエンスコーパス: dimethyl sulfate

1 treating primary oviduct cell cultures with dimethyl sulfate.

2 as more classical reagents, such as Pb2+ or dimethyl sulfate.

3 lation of the respective diamidoxime 5a with dimethyl sulfate.

4 accelerated by replacing methyl iodide with dimethyl sulfate.

5 mple, diagnostic alkylation of guanine N7 by dimethyl sulfate.

6 nhibition is observed for DNA methylation by dimethyl sulfate.

7 and chemical modification interference with dimethyl sulfate, 1-cyclohexyl-3-(2-morpholinoethyl)carb

8 ins generally have higher reactivity to DMS (dimethyl sulfate), a chemical that covalently modifies a

9 proof of concept, we present high-throughput dimethyl sulfate accessibility data for a chimeric DNA/R

10 m riboswitch with N-methylisatoic anhydride, dimethyl sulfate and 1-cyclohexyl-3-(2-morpholinoethyl)c

11 robing of the P4-P6 tertiary structure using dimethyl sulfate and CMCT confirms that these TGGE exper

12 by electrophoretic mobility shift assays and dimethyl sulfate and diethyl pyrocarbonate interference

13 ing, chemical modification experiments using dimethyl sulfate and hydrazine were performed on both th

14 his study, we used a combination of DNase I, dimethyl sulfate and hydroxyl radical footprinting analy

15 edly demonstrated an average reactivity with dimethyl sulfate and minimal reactivity with RNase T1, t

16 This prediction was tested by using dimethyl sulfate and potassium permanganate footprinting

17 uch lower level by the SN2 methylating agent dimethyl sulfate and repaired much faster than 7MeGs in

18 For the procedure, gramine is treated with dimethyl sulfate and sodium in ethanol at room temperatu

19 We probed the structure of 13:22 by dimethyl sulfate and tested its partner in a base-triple

20 hyl) carbodiimide metho-p-toluene sulfonate, dimethyl sulfate, and kethoxal.

21 Chemical probing with chloroacetaldehyde, dimethyl sulfate, and potassium permanganate is consiste

22 groove-specific interactions, as detected by dimethyl sulfate, are diminished.

23 ctivity results in increased mutagenicity of dimethyl sulfate as evidenced by a 2-fold increase in La

24 on, surrounding C2394, a base protected from dimethyl sulfate by E site tRNA, and in the phylogenetic

25 thylpurine-DNA glycosylase (MPG protein) and dimethyl sulfate-damaged DNA manifested sequence context

26 on nuclear protein-DNA interactions by using dimethyl sulfate (DMS) and DNase I ligation-mediated PCR

27 in infected cells by in vivo treatment with dimethyl sulfate (DMS) followed by visualization through

28 d nuclei, the rmm3 rDNA lacked the wild-type dimethyl sulfate (DMS) footprint in the promoter region

29 is tract both in vitro and in vivo using the dimethyl sulfate (DMS) footprinting technique and nucleo

30 analysis of the G-rich strand combined with dimethyl sulfate (DMS) footprinting, a polymerase stop a

31 lar dichroism (CD), thermal denaturation and dimethyl sulfate (DMS) footprinting, we found that a sin

32 h strand DNA of NHE was identified by CD and dimethyl sulfate (DMS) footprinting.

33 e of comparing this method with the standard dimethyl sulfate (DMS) in vivo method and previously rep

34 mobility shift assays, DNase I footprinting, dimethyl sulfate (DMS) interference assays, and DMS prot

35 For decades, dimethyl sulfate (DMS) mapping has informed manual model

36 We describe a protocol in which dimethyl sulfate (DMS) modification of the base-pairing

37 Here we present dimethyl sulfate (DMS) mutational profiling with sequenc

38 Mg(2+) using both the traditional method of dimethyl sulfate (DMS) N1 methylation and a new approach

39 forms a Py.Pu.Py triplex as detected by both dimethyl sulfate (DMS) probing and a gel-shift assay; in

40 otides and then detects these events through dimethyl sulfate (DMS) probing and mutational profiling.

41 Dimethyl sulfate (DMS) probing of the cross-linked d[GCC

42 etermine aptamer sensitivity/selectivity and dimethyl sulfate (DMS) probing to explore aptamer bindin

43 Using time-resolved dimethyl sulfate (DMS) probing, we have analyzed time-de

44 ng the gel shift assay, chemical probing and dimethyl sulfate (DMS) protection studies, we determined

45 nd the same intracellular eukaryotic mRNA by dimethyl sulfate (DMS) structure probing.

46 We show through chemical probing with dimethyl sulfate (DMS) that conformational changes occur

47 Solvent accessibility reagents, such as dimethyl sulfate (DMS), 1-cyclohexyl-3-(2-morpholinoethy

48 and A1493 are protected from reactivity with dimethyl sulfate (DMS).

49 thylpyrocarbonate (DEPC; to probe A at N-7), dimethyl sulfate (DMS; to probe A at N-1, and C at N-3),

50 ween the two guanosines protected by in vivo dimethyl sulfate DNA footprinting (GAAGAGTG) in a lucife

51 ligation-mediated PCR combined with in vivo dimethyl sulfate, DNase I, or UV treatment-of ICR sequen

52 Dimethyl sulfate footprint analysis confirmed that the i

53 In vivo dimethyl sulfate footprint analysis confirmed the existe

54 In vivo dimethyl sulfate footprinting analysis of the CUP1 promo

55 We found here, through in vitro dimethyl sulfate footprinting and gel mobility shift ass

56 We show by dimethyl sulfate footprinting and RNA polymerase arrest

57 ques including DNase I, hydroxyl radical and dimethyl sulfate footprinting and the circular permutati

58 Electrophoretic mobility shift and dimethyl sulfate footprinting assays demonstrated that a

59 We used in vivo dimethyl sulfate footprinting by ligation-mediated PCR t

60 However, in vivo dimethyl sulfate footprinting demonstrated that protein-

61 an beta-globin locus, we analyzed by in vivo dimethyl sulfate footprinting erythroid cells expressing

62 In vivo dimethyl sulfate footprinting of the cyclin E promoter r

63 In vivo dimethyl sulfate footprinting of the HS-2 region reveale

64 First, in vivo dimethyl sulfate footprinting of the human LDL receptor

65 quence using a Taq polymerase stop assay and dimethyl sulfate footprinting revealed the formation of

66 Hydroxyl radical and dimethyl sulfate footprinting show that both I(trap) and

67 Dimethyl sulfate footprinting showed that the major groo

68 By using dimethyl sulfate footprinting, we recently identified tw

69 ng crudely similar complexes, as revealed by dimethyl sulfate footprinting.

70 A comparison of DNase I and dimethyl sulfate footprints in vivo and in vitro strongl

71 method reported here demonstrates the use of dimethyl sulfate for conversion of enaminoketones to bet

72 footprinting showed similar accessibility to dimethyl sulfate for PU.1/DNA and Ets-1/DNA complexes, i

73 se T1, RNAse V1, RNAse U2, lead acetate, and dimethyl sulfate has led to the generation of the first

74 ractions in this promoter were identified by dimethyl sulfate in vivo footprinting analysis of NG108-

75 Dimethyl sulfate in vivo footprinting identified 10 puta

76 se III mediated in vivo DNA footprinting and dimethyl sulfate in vivo footprinting revealed DNA prote

77 reased methylation of the N7 of guanines (by dimethyl sulfate) in the zinc finger contacts of the ICR

78 gle-stranded DNA-specific reagents KMnO4 and dimethyl sulfate indicated that RecBCD opened, in a Mg(2

79 Base excision repair of dimethyl sulfate induced N-methylpurines (NMPs) was meas

80 Base excision repair rates of dimethyl sulfate-induced 3-methyladenine and 7-methylgua

81 issing-nucleoside interference experiment, a dimethyl sulfate interference experiment, and an examina

82 Potassium permanganate and dimethyl sulfate interference experiments showed that RX

83 Potassium permanganate and dimethyl sulfate interference experiments using the AIIA

84 Induction of 7MeGs by dimethyl sulfate is affected by nearest-neighbor nucleot

85 The versatility of CAFA is illustrated by dimethyl sulfate mapping of RNA secondary structure and

86 ll occupied, are less protected from in vivo dimethyl sulfate methylation in a deltaGRF2 strain.

87 Dimethyl sulfate methylation interference assays indicat

88 Moreover, dimethyl sulfate methylation protection assays demonstra

89 ppears to form a stem-loop in vivo, based on dimethyl sulfate modification and the sequences of intra

90 In vivo dimethyl sulfate modification of ribosomal RNA and the d

91 We used dimethyl sulfate modification protection studies to show

92 Using time-dependent dimethyl sulfate modification, we have determined that a

93 ppears to bind proteins that protect it from dimethyl sulfate modification.

94 on factor binding sites identified by either dimethyl sulfate or DNase I in vivo footprinting of the

95 ethylation by treatment of cells or DNA with dimethyl sulfate or from reaction of DNA with DB[a,l]P i

96 was accomplished by treatment of cells with dimethyl sulfate or ultraviolet light, followed by ligat

97 Dimethyl sulfate probing results suggest that the compac

98 n living yeast (Saccharomyces cerevisiae) by dimethyl sulfate probing with or without Rev.

99 Dimethyl sulfate protection assays revealed limited cont

100 several areas of protein binding as shown by dimethyl sulfate protection or enhancement.

101 complex with 1, 10-phenanthroline-copper and dimethyl sulfate protection reveal that both the heptame

102 aracterize these conformational states using dimethyl sulfate reactivity studies and Bal 31 nuclease

103 of unpaired adenine and cytosine residues by dimethyl sulfate, result in a stop in reverse transcript

104 ease-sensitive sites in vitro and by in vivo dimethyl sulfate RNA modification.

105 Using native gel electrophoresis and dimethyl sulfate structural probing, we monitored Mss-11

106 this, we used in vivo chemical probing with dimethyl sulfate to detect changes in pre-rRNA structure

107 fold independently, we used Fe(II)-EDTA and dimethyl sulfate to probe the solvent accessibility of s

108 Using ligation-mediated PCR on in vivo dimethyl sulfate-treated HTLV-1-infected cell lines MT-2

109 an in vivo footprinting method that couples dimethyl sulfate treatment and ligation-mediated PCR was

110 tifs from DNase I digestion or reaction with dimethyl sulfate was observed and phenobarbital treatmen

111 obtain a map of potential interaction sites, dimethyl sulfate was used to footprint regions of the in

112 Using both Fe(II).EDTA and dimethyl sulfate, we have identified a strong footprint