ライフサイエンスコーパス: dinitrogenase

1 rone to the FeMo-co-deficient apo-NifDK (apo-dinitrogenase).

2 ifD1 and nifK1, which together encode the Mo dinitrogenase.

3 e the only previous example was that seen in dinitrogenase.

4 acetylene reduction ability of the resulting dinitrogenase.

5 red for its function in electron transfer to dinitrogenase.

6 r (FeMo-co) of the widely studied molybdenum-dinitrogenase.

7 omplex, forming a tight protein complex with dinitrogenase.

8 ignals of molybdenum- or vanadium-containing dinitrogenases.

9 rogenase 1 (a catalytically inactive form of dinitrogenase 1 that lacks the FeMo-co) to the FeMo-co-a

10 rogenase 2 (a catalytically inactive form of dinitrogenase 2 that lacks FeV-co) exhibit only the VNFG

11 cts of A. vinelandii strains that accumulate dinitrogenase 2, whereas extracts of strains impaired in

12 n be correlated with the formation of active dinitrogenase 2.

13 trogenase 2 results in its reconstitution to dinitrogenase 2.

14 processing apodinitrogenase 2 to functional dinitrogenase 2.

15 ertion mutation in vnfY has 10-fold less vnf dinitrogenase activity and exhibits a greatly diminished

16 enase reductase activity is lowered, whereas dinitrogenase activity remains essentially unaltered.

17 ected in diazotrophic growth and extractable dinitrogenase activity when cultured under conditions th

18 alancing systems, the Calvin-Benson-Bassham, dinitrogenase and dimethyl sulfoxide reductase systems,

19 vealed distinct clusters for Mo-, V-, and Fe-dinitrogenases and suggested that most methanogens also

20 ogenase when present in a 1:1 molar ratio to dinitrogenase, and 2OG fully relieved this inhibition.

21 ated between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in ni

22 nd for the maturation of alpha(2)beta(2) apo-dinitrogenase (apo-dinitrogenase maturation).

23 -loop of NifI(2) prevented copurification of dinitrogenase but did not affect copurification of NifI(

24 rect interaction of a NifI(1,2) complex with dinitrogenase causes inhibition, which is relieved by 2O

25 es serving as the obligate electron donor to dinitrogenase during nitrogenase turnover, dinitrogenase

26 In addition to reducing dinitrogenase during nitrogenase turnover, NIFH function

27 about 23 kb from vnfDGK, which encodes the V dinitrogenase; however, like vnfDGK, vnfH was expressed

28 binding between dinitrogenase reductase and dinitrogenase; however, this change did not have a subst

29 espond to 2OG and ATP, and bind and regulate dinitrogenase in Euryarchaeota and many Bacteria.

30 Dinitrogenase is a heterotetrameric (alpha(2)beta(2)) en

31 lectron flux from dinitrogenase reductase to dinitrogenase is also surprisingly insensitive to a depl

32 synthesize FeMo-co accumulate an apo form of dinitrogenase (lacking FeMo-co), with a subunit composit

33 A factor capable of activating apo-dinitrogenase (lacking the FeMo cofactor) from Azotobact

34 on of alpha(2)beta(2) apo-dinitrogenase (apo-dinitrogenase maturation).

35 rving as the physiological electron donor to dinitrogenase, NifH is involved in iron-molybdenum cofac

36 o its role as the obligate electron donor to dinitrogenase, NifH is required for the iron-molybdenum

37 99Mo label accumulates on dinitrogenase only when all known components of the FeMo

38 The dinitrogenase protein (ANF1) contains three subunits in

39 n paramagnetic resonance (EPR) signal of the dinitrogenase protein is not characteristic of the EPR s

40 inelandii was extracted from the alternative dinitrogenase protein with N-methylformamide.

41 hesized apo-dinitrogenase to generate mature dinitrogenase protein.

42 The alternative dinitrogenase reductase (ANF2) was purified as an alpha2

43 o dinitrogenase during nitrogenase turnover, dinitrogenase reductase (NifH) is required for the biosy

44 (the metabolic product of NifB), NifNE, and dinitrogenase reductase (NifH).

45 nitrogenase reductase ADP-ribosyltransferase/dinitrogenase reductase activating glycohydrolase (DRAT/

46 In these mutants, dinitrogenase reductase activity is lowered, whereas din

47 of dinitrogenase reductase catalyzed by the dinitrogenase reductase ADP-ribosyl transferase (DRAT)/d

48 bolism, including GlnE, NtrB/NtrC, and DRAT (dinitrogenase reductase ADP-ribosyl transferase)-DRAG (d

49 of dinitrogenase reductase, catalyzed by the dinitrogenase reductase ADP-ribosyl transferase-dinitrog

50 The nitrogenase-regulating enzymes dinitrogenase reductase ADP-ribosyltransferase (DRAT) an

51 cross-linking of dinitrogenase reductase and dinitrogenase reductase ADP-ribosyltransferase (DRAT) fr

52 be ADP-ribosylated or to form a complex with dinitrogenase reductase ADP-ribosyltransferase (DRAT) fr

53 ctase) activity is reversibly inactivated by dinitrogenase reductase ADP-ribosyltransferase (DraT) in

54 eductase is posttranslationally regulated by dinitrogenase reductase ADP-ribosyltransferase (DRAT) vi

55 P-ribosylation of dinitrogenase reductase by dinitrogenase reductase ADP-ribosyltransferase (DRAT).

56 The dra operon encodes dinitrogenase reductase ADP-ribosyltransferase and dinit

57 ADP-ribosylation catalyzed by the DRAT-DRAG (dinitrogenase reductase ADP-ribosyltransferase-dinitroge

58 eir nitrogenase activity, independent of the dinitrogenase reductase ADP-ribosyltransferase/dinitroge

59 Chemical cross-linking of dinitrogenase reductase and dinitrogenase reductase ADP-

60 Lys 143 to Gln decreased the binding between dinitrogenase reductase and dinitrogenase; however, this

61 Cross-linking between dinitrogenase reductase and DRAT requires the presence o

62 The R101F form of dinitrogenase reductase and DRAT together were not able

63 um chloride, as is the cross-linking between dinitrogenase reductase and DRAT, suggesting that ionic

64 reaction, does support cross-linking between dinitrogenase reductase and DRAT.

65 ubstantial effect on the interaction between dinitrogenase reductase and DRAT.

66 significantly change the interaction between dinitrogenase reductase and DRAT.

67 It is also possible that dinitrogenase reductase and/or NIFNE (both Fe-S proteins

68 e reduction assays, immunoblotting with anti-dinitrogenase reductase antibody, and [adenylate-(32)P]N

69 olve detectable covalent modification of the dinitrogenase reductase as in some bacteria, and the gen

70 1 is not critical for the binding of DRAT to dinitrogenase reductase but that the availability of arg

71 nslationally through the ADP-ribosylation of dinitrogenase reductase by dinitrogenase reductase ADP-r

72 pression and posttranslational regulation of dinitrogenase reductase by reversible ADP-ribosylation c

73 Both DRAT and dinitrogenase reductase can be labeled by [carbonyl-(14)

74 lation of the reversible ADP ribosylation of dinitrogenase reductase catalyzed by the dinitrogenase r

75 as the transcriptional regulator SoxR or the dinitrogenase reductase component of nitrogenase.

76 R140Q dinitrogenase reductase could not be ADP-ribosylated by

77 lation or any other covalent modification of dinitrogenase reductase during switch-off, suggesting th

78 f an arginine residue at position 101 in the dinitrogenase reductase eliminated this ADP-ribosylation

79 at both oxygen-denatured and ADP-ribosylated dinitrogenase reductase fail to form a cross-linked comp

80 -bound and adenine nucleotide-free states of dinitrogenase reductase form cross-linked complexes with

81 In this study, mutant forms of dinitrogenase reductase from A. vinelandii that are affe

82 The structure of the dinitrogenase reductase from Azotobacter vinelandii is k

83 and in the ability to recognize variants of dinitrogenase reductase have been found.

84 ither lost nitrogenase activity nor modified dinitrogenase reductase in response to darkness and NH4+

85 rogenase activity and normal modification of dinitrogenase reductase in response to NH(4)(+) and dark

86 Conformational changes in dinitrogenase reductase induced by an F135Y substitution

87 AT; however, cross-linking is inhibited when dinitrogenase reductase is in its ATP-bound state.

88 The DRAT-catalyzed ADP-ribosylation of dinitrogenase reductase is inhibited by sodium chloride,

89 Dinitrogenase reductase is posttranslationally regulated

90 H4+, suggesting that the ADP-ribosylation of dinitrogenase reductase is probably the only mechanism f

91 removal of the Fe(4)S(4) cluster resulted in dinitrogenase reductase not being a substrate for ADP-ri

92 The nifH1 gene, which encodes the dinitrogenase reductase of the Mo nitrogenase that is ex

93 The vnfH gene, which encodes the dinitrogenase reductase of the V nitrogenase, was locate

94 Thus, the Arg 140 residue of dinitrogenase reductase plays a critical role in the ADP

95 Although ADP-ribosylation of dinitrogenase reductase plays a significant role in the

96 reversible covalent ADP-ribosylation of the dinitrogenase reductase protein, NifH.

97 t nitrogenase 2 (vnf-encoded) has a distinct dinitrogenase reductase protein, VNFH.

98 These results suggest that the dinitrogenase reductase proteins do not specify the hete

99 The strain containing the R101F form of dinitrogenase reductase retains 91%, the strain containi

100 The ability of R101F dinitrogenase reductase to be labeled by [carbonyl-(14)C

101 ws that the ATP-dependent electron flux from dinitrogenase reductase to dinitrogenase is also surpris

102 that these changes decreased the ability of dinitrogenase reductase to form a cross-linkable complex

103 rubrum strains in which the arginine 101 of dinitrogenase reductase was replaced by tyrosine, phenyl

104 The R101F and R101Y forms of dinitrogenase reductase were able to form a complex with

105 genase activity of the strain containing the dinitrogenase reductase with arginine at position 101.

106 Fe protein (dinitrogenase reductase) activity is reversibly inactiva

107 red for FeMo-co biosynthesis (e.g. NIFNE and dinitrogenase reductase) are at the appropriate redox st

108 NifH (dinitrogenase reductase) has three important roles in th

109 lum rubrum strain that lacked its endogenous dinitrogenase reductase, and they supported high nitroge

110 Reversible ADP-ribosylation of dinitrogenase reductase, catalyzed by the dinitrogenase

111 s-linking is specific for native, unmodified dinitrogenase reductase, in that both oxygen-denatured a

112 in nif expression or the ADP-ribosylation of dinitrogenase reductase, since a mutant expressing GS-Y3

113 The ADP-ribosyl is removed by the dinitrogenase reductase-activating glycohydrolase (DraG)

114 G gene was used to generate altered forms of dinitrogenase reductase-activating glycohydrolase (DRAG)

115 ase reductase ADP-ribosyl transferase (DRAT)/dinitrogenase reductase-activating glycohydrolase (DRAG)

116 reductase ADP-ribosyltransferase (DRAT) and dinitrogenase reductase-activating glycohydrolase (DRAG)

117 Association of dinitrogenase reductase-activating glycohydrolase (DRAG)

118 itrogenase reductase ADP-ribosyl transferase-dinitrogenase reductase-activating glycohydrolase (DRAT-

119 nitrogenase reductase ADP-ribosyltransferase-dinitrogenase reductase-activating glycohydrolase) syste

120 ase reductase ADP-ribosyl transferase)-DRAG (dinitrogenase reductase-activating glycohydrolase).

121 ogenase reductase ADP-ribosyltransferase and dinitrogenase reductase-activating glycohydrolase, enzym

122 regulated by reversible ADP-ribosylation of dinitrogenase reductase.

123 These mutated dinitrogenase reductases also were expressed in a Rhodos

124 or NifH in the Mo nitrogenase and that these dinitrogenase reductases are not involved in determining

125 iption of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-

126 Altered dinitrogenase reductases from strains UR629 (R101Y) and

127 e mutants and no ADP-ribosylation of altered dinitrogenase reductases occurred either in vivo or in v

128 altered nucleotide binding regions of these dinitrogenase reductases, did not significantly change t

129 exhibited an EPR spectrum characteristic of dinitrogenase reductases.

130 The formation of an active dinitrogenase requires the synthesis and the insertion o

131 esulted in copurification of NifI(1) and the dinitrogenase subunits NifD and NifK, and 2OG or a delet

132 ession of both the Calvin-Benson-Bassham and dinitrogenase systems was influenced by dimethyl sulfoxi

133 n interaction between purified NifI(1,2) and dinitrogenase that was inhibited by 2OG.

134 ls for extracted FeMo-co and the M center of dinitrogenase; third, site-directed mutagenesis of nafY

135 finally inserted into a pre-synthesized apo-dinitrogenase to generate mature dinitrogenase protein.

136 nthesis of FeMo-co and the activation of apo-dinitrogenase under anaerobic-reducing conditions.

137 of the iron-molybdenum cofactor (FeMo-co) of dinitrogenase was investigated using 99Mo to follow the

138 9)V label incorporation into the V-dependent dinitrogenase when compared to the wild type.

139 a role in the maturation of the V-dependent dinitrogenase, with a specific role in the formation of