ライフサイエンスコーパス: diphtheriae

1 tatic and virulence genes in Corynebacterium diphtheriae.

2 nes were constructed on the chromosome of C. diphtheriae.

3 of known virulence genes in Corynebacterium diphtheriae.

4 ed the precise amount of DtxR per cell in C. diphtheriae.

5 than another studied HO from Corynebacterium diphtheriae.

6 l of iron-sensitive genes in Corynebacterium diphtheriae.

7 l ion-activated repressor in Corynebacterium diphtheriae.

8 cobacterium tuberculosis and Corynebacterium diphtheriae.

9 ecretion of diphtheria toxin by wild-type C. diphtheriae.

10 n(2+) transport systems may be present in C. diphtheriae.

11 egulated promoters (IRPs) in Corynebacterium diphtheriae.

12 loregulatory proteins to be identified in C. diphtheriae.

13 t time that dtxR is a dispensable gene in C. diphtheriae.

14 tem previously identified in Corynebacterium diphtheriae.

15 nose swab to be positive for Corynebacterium diphtheriae.

16 the general oxidative folding machine in C. diphtheriae.

17 mmunicable disease caused by Corynebacterium diphtheriae.

18 ane when expressed in Escherichi coli and C. diphtheriae.

19 nd may function as the haemin receptor in C. diphtheriae.

20 d the need for laboratories to screen for C. diphtheriae.

21 ay a crucial role in the pathogenicity of C. diphtheriae.

22 in from toxigenic strains of Corynebacterium diphtheriae.

23 emoglobin as iron sources by Corynebacterium diphtheriae.

24 al host iron sources that are utilized by C. diphtheriae.

25 negative global regulator in Corynebacterium diphtheriae.

26 -TOF MS) were conclusive for Corynebacterium diphtheriae.

27 ad no effect on hemin iron utilization in C. diphtheriae.

28 virulence and other genes in Corynebacterium diphtheriae.

29 se reaction intermediates in Corynebacterium diphtheriae.

30 nction as cell surface hemin receptors in C. diphtheriae.

31 currently the method of choice for typing C. diphtheriae.

32 h ferrous ion homeostasis in Corynebacterium diphtheriae.

33 of the regulation of heme homeostasis in C. diphtheriae.

34 gnition of the toxin gene in Corynebacterium diphtheriae.

35 mmalian HO-1 and the HO from Corynebacterium diphtheriae.

36 antigenically distinct from other pili of C. diphtheriae.

37 scheme, which is currently restricted to C. diphtheriae.

38 sights into transcriptional regulation in C. diphtheriae.

39 he SpaABC pili and possibly other pili of C. diphtheriae.

40 n linked to the virulence of Corynebacterium diphtheriae.

41 iosynthesis and transport in Corynebacterium diphtheriae.

42 esent a potential reservoir for toxigenic C. diphtheriae.

43 onsillectomy and immunity to Corynebacterium diphtheriae (1931), 2 papers from a longitudinal study o

44 r the clinically significant Corynebacterium diphtheriae (4 of 4) and Corynebacterium jeikeium (8 of

45 To further define the DtxR regulon in C. diphtheriae, a DtxR repressor titration assay (DRTA) was

46 ria toxin (Dtx) expressed by Corynebacterium diphtheriae also can function as part of an anti-predato

47 me oxygenase mutants of both Corynebacterium diphtheriae and C. ulcerans fail to use heme as an iron

48 C. diphtheriae and C. ulcerans mutants defective in haemin

49 etions in the hmuO gene from Corynebacterium diphtheriae and Corynebacterium ulcerans and show that t

50 Nontoxigenic Corynebacterium diphtheriae and Corynebacterium ulcerans cause invasive

51 Mutants of C. diphtheriae and Corynebacterium ulcerans that are defect

52 Corynebacterium diphtheriae and Corynebacterium ulcerans use haemin and

53 ection caused by a nontoxigenic strain of C. diphtheriae and discuss the epidemiology, possible sourc

54 of the SpaA-type pilus from Corynebacterium diphtheriae and FimA of the type 2 pilus from Actinomyce

55 m the gram-positive pathogen Corynebacterium diphtheriae and for eukaryotic heme oxygenases.

56 inc specific transcriptional regulator in C. diphtheriae and give new insights into the intricate reg

57 are and contrast galactan biosynthesis in C. diphtheriae and M. tuberculosis In each species, the gal

58 n of amino acids, as well as Corynebacterium diphtheriae and Mycobacterium tuberculosis, which cause

59 diphthamide, the target for Corynebacterium diphtheriae and Pseudomonas aeruginosa toxins.

60 d by a lack of molecular genetic tools in C. diphtheriae and related Coryneform species.

61 utilization of heme as an iron source by C. diphtheriae and that the heme oxygenase activity of HmuO

62 -based mutagenesis technique for use with C. diphtheriae, and we used it to construct the first trans

63 Diagnostic tests for toxinogenicity of C. diphtheriae are based either on immunoassays or on bioas

64 We show here that pili of Corynebacterium diphtheriae are composed of three pilin subunits, SpaA,

65 ix sortase genes encoded in the genome of C. diphtheriae are required for precursor processing, pilus

66 toxin repressor (DtxR) from Corynebacterium diphtheriae, are iron-dependent regulatory proteins that

67 Corynebacterium diphtheriae assembles on its surface three distinct pilu

68 ing classified the strain as nontoxigenic C. diphtheriae biotype Gravis.

69 cobacterium tuberculosis and Corynebacterium diphtheriae, but unlike the linear chromosomes of the mo

70 new IRP, designated IRP6, was cloned from C. diphtheriae by a SELEX-like procedure.

71 Inactivation of hmuT in C. diphtheriae by site-specific recombination had no effect

72 ized with either pentavalent Corynebacterium diphtheriae C7 (beta197) cross-reactive material (CRM197

73 operon contained a large deletion in the C. diphtheriae C7 strain, but the sid genes were unaffected

74 C. diphtheriae C7(-) acquired iron from heme, hemoglobin, a

75 d under high-iron conditions in wild-type C. diphtheriae C7(beta), but they were expressed constituti

76 In C. diphtheriae C7, optimal expression from the hmuO promote

77 xR(E175K) mutant allele from Corynebacterium diphtheriae can be expressed in Mycobacterium tuberculos

78 None of the eight C. diphtheriae cases were fully immunized.

79 ation enzyme in the pathogen Corynebacterium diphtheriae, catalyzes the oxygen-dependent conversion o

80 Toxigenic strains of Corynebacterium diphtheriae cause respiratory diphtheria.

81 eted by lysogenic strains of Corynebacterium diphtheriae, causes the disease diphtheria in humans by

82 tants in heme oxygenase from Corynebacterium diphtheriae (cd-HO).

83 reductase A of the pathogen Corynebacterium diphtheriae (Cd-MsrA) and shown that this enzyme is coup

84 r-lacZ fusion was dependent on the cloned C. diphtheriae chrA and chrS genes (chrAS), which encode th

85 In this study, two clones isolated from a C. diphtheriae chromosomal library were shown to activate t

86 ephages are capable of inserting into the C. diphtheriae chromosome at two specific sites, attB1 and

87 each of these systems was cloned from the C. diphtheriae chromosome, and constructs each carrying one

88 organism showed that several genotypes of C. diphtheriae circulated on different continents of the wo

89 ed the predominant strain of nontoxigenic C. diphtheriae circulating in the United Kingdom to see if

90 C. diphtheriae ciuE deletion mutants exhibited a defect in

91 e, we characterized a large collection of C. diphtheriae clinical isolates for their pilin gene pool

92 he findings from this study indicate that C. diphtheriae contains at least 18 DtxR binding sites and

93 The genome of Corynebacterium diphtheriae contains three pilus gene clusters, two of w

94 toxin gene CRM197 allele in Corynebacterium diphtheriae culture DNA samples.

95 e complemented the mutant phenotypes of a C. diphtheriae DeltadtxR strain.

96 al DtxR-regulated promoter/operators from C. diphtheriae designated IRP3, IRP4, and IRP5.

97 aR, with 26% identity to the Corynebacterium diphtheriae diphtheria toxin repressor (DtxR).

98 ein distantly related to the Corynebacterium diphtheriae diphtheria toxin repressor (DtxR).

99 In Corynebacterium diphtheriae, diphtheria toxin is encoded by the tox gene

100 Although C. diphtheriae does not depend on other actinomycetal genes

101 Corynebacterium diphtheriae DtxR is an iron-specific repressor of diphth

102 omologous to elements of the Corynebacterium diphtheriae DtxR regulon, which controls, in response to

103 In Corynebacterium diphtheriae, DtxR regulates not only the expression of d

104 tal-dependent regulator from Corynebacterium diphtheriae, DtxR.

105 Thus, C. diphtheriae employs a common sortase-catalysed mechanism

106 ings demonstrated that the irp6 operon in C. diphtheriae encodes a putative ABC transporter, that spe

107 Corynebacterium diphtheriae encodes six sortases, five of which are devo

108 egmatis (EsxA and EsxB), and Corynebacterium diphtheriae (EsxA and EsxB) are heterodimers and fold in

109 The Gram-positive pathogen Corynebacterium diphtheriae exports through the Sec apparatus many extra

110 In this study, we describe a Corynebacterium diphtheriae ferric uptake regulator-family protein, Zur,

111 a heme degradation enzyme in Corynebacterium diphtheriae, forms a stoichiometric complex with iron pr

112 transporter involved in the protection of C. diphtheriae from hemin toxicity.

113 resses the clone's origin and relation to C. diphtheriae from throughout Russia.

114 ts and on the results of experiments with C. diphtheriae genes cloned in Escherichia coli or analyzed

115 A search of the partially completed C. diphtheriae genome identified a gene, mntR, whose predic

116 A BLAST search of the C. diphtheriae genome identified a seven-gene cluster that

117 A BLAST search of the C. diphtheriae genome sequence revealed a second two-compon

118 The hrtAB genes are located on the C. diphtheriae genome upstream from the chrSA operon, which

119 Four clones from a C. diphtheriae genomic library complemented several of the

120 The C. diphtheriae GlfT2 gave rise to shorter polysaccharides t

121 on hemin utilization, which suggests that C. diphtheriae has an additional system for transporting he

122 olecular characterization of Corynebacterium diphtheriae has become a priority in order to be able to

123 ulation of endemic toxigenic Corynebacterium diphtheriae has been identified.

124 Corynebacterium diphtheriae has been shown to assemble a pilus structure

125 rom the pathogenic bacterium Corynebacterium diphtheriae has been subcloned and expressed in Escheric

126 iology of diseases caused by Corynebacterium diphtheriae has changed dramatically over the decades, a

127 f tools, genetic analysis of Corynebacterium diphtheriae has primarily relied on analysis of chemical

128 rom the pathogenic bacterium Corynebacterium diphtheriae, have been investigated by (1)H NMR spectros

129 txR mutant of C7, and in a hmuO mutant of C. diphtheriae HC1 provided further evidence that transcrip

130 nation and spin-state of the Corynebacterium diphtheriae heme oxygenase (Hmu O) and the proximal Hmu

131 l alpha-meso-hydroxylation of heme in the C. diphtheriae heme oxygenase-catalyzed reaction.

132 The C. diphtheriae hmuO gene encodes a heme oxygenase that is i

133 The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is i

134 Transcription of the Corynebacterium diphtheriae hmuO gene, which encodes a heme oxygenase in

135 n and heme whereas transcription from the C. diphtheriae hmuO promoter shows both significant iron re

136 ces from the mammalian HO-1 and bacterial C. diphtheriae HO structures, which suggests a structural b

137 endent activation at the hmuO promoter in C. diphtheriae; however, it was observed that significant l

138 report, we identify and characterize the C. diphtheriae hrtAB genes, which encode a putative ABC typ

139 Molecular subtyping of Corynebacterium diphtheriae identified significant genetic diversity wit

140 h a proposed mechanism of hemin uptake in C. diphtheriae in which hemin is initially obtained from Hb

141 n of iron-sensitive genes in Corynebacterium diphtheriae, including the diphtheria toxin gene.

142 Two (9.5%) cases died, one due to a C. diphtheriae infection and one due to C. ulcerans.

143 nerstone of the treatment of Corynebacterium diphtheriae infection for more than 100 years.

144 orted the improbability of importation of C. diphtheriae into this area and rather strongly suggest t

145 The use of hemin iron in C. diphtheriae involves the dtxR- and iron-regulated hmu he

146 erial IdeR, a homolog of the Corynebacterium diphtheriae iron regulator DtxR.

147 , represses transcription of Corynebacterium diphtheriae iron-regulated promoters in vivo and binds t

148 The Corynebacterium diphtheriae irp1 gene is negatively regulated by DtxR an

149 toxin repressor (DtxR) from Corynebacterium diphtheriae is a divalent metal-activated repressor of c

150 Corynebacterium diphtheriae is a Gram-positive, non-spore forming, non-m

151 of the pathogenic bacterium Corynebacterium diphtheriae is conferred by diphtheria toxin, whose expr

152 Transcription of the hmuO gene in C. diphtheriae is controlled under a dual regulatory mechan

153 DtxR of Corynebacterium diphtheriae is the best characterized of these important

154 iptional regulator DtxR from Corynebacterium diphtheriae is the prototype for a family of metal-depen

155 toxin repressor (DtxR) from Corynebacterium diphtheriae is the prototypic member of a superfamily of

156 The toxigenic C. diphtheriae isolate NCTC13129 produces three distinct he

157 A distinct clonal group of C. diphtheriae isolates (ET 8 complex) emerged in Russia in

158 tion of 53 U.S. and Canadian Corynebacterium diphtheriae isolates by multilocus enzyme electrophoresi

159 tive composite transposon associated with C. diphtheriae isolates that dominated the diphtheria outbr

160 corynephages, and DT is only produced by C. diphtheriae isolates that harbor tox+ phages.

161 In a blinded test of 209 verified C. diphtheriae isolates, a 99.5% agreement with the standar

162 d for the differentiation of Corynebacterium diphtheriae isolates.

163 A C. diphtheriae mntA mutant grew as well as the wild type in

164 min iron utilization assays using various C. diphtheriae mutants indicate that deletion of the chtA-c

165 the C. ulcerans mutants and three of the C. diphtheriae mutants.

166 tP site and the DIP0182 integrase gene of C. diphtheriae NCTC13129.

167 m were infected by toxigenic Corynebacterium diphtheriae of both mitis and gravis biotypes, showing t

168 -regulated promoters in vivo and binds to C. diphtheriae operators in a metal-dependent manner in vit

169 he source of galactan length variation, a C. diphtheriae ortholog of the M. tuberculosis carbohydrate

170 l eight tox regions identical to those of C. diphtheriae Park-Williams No. 8 (tox type 1).

171 y was caused by one major clonal group of C. diphtheriae (PFGE type A, ribotype R1), which was identi

172 heme and hemoglobin, which suggests that C. diphtheriae possesses a novel mechanism for utilizing he

173 Therefore, recent clinical isolates of C. diphtheriae produce a single antigenic type of DT, and d

174 Deletion of the hrtAB genes in C. diphtheriae produced increased sensitivity to hemin, whi

175 derophore, and several other Corynebacterium diphtheriae products.

176 Nontoxigenic strains of Corynebacterium diphtheriae represent a potential reservoir for the emer

177 of the Hb-Hp complex as an iron source by C. diphtheriae requires multiple iron-regulated surface com

178 The use of hemin iron by Corynebacterium diphtheriae requires the DtxR- and iron-regulated ABC he

179 Pilus assembly in C. diphtheriae requires the pilin motif and the C-terminal

180 f MdbA or a counterpart from Corynebacterium diphtheriae, rescues the Deltavkor mutant.

181 muO, the heme oxygenase from Corynebacterium diphtheriae, restores iron and heme levels, as well as A

182 dtxR genes in recent clinical isolates of C. diphtheriae revealed several tox alleles that encode ide

183 thogenic bacteria, including Corynebacterium diphtheriae, Salmonella enterica, and Vibrio cholerae, a

184 Protein localization studies with C. diphtheriae showed that HtaA is associated predominantly

185 on iron uptake or the utilization of the C. diphtheriae siderophore as an iron source.

186 ron uptake and reduced ability to use the C. diphtheriae siderophore as an iron source.

187 ved in the synthesis and transport of the C. diphtheriae siderophore.

188 Corynebacterium diphtheriae SpaA pili are composed of three pilin subuni

189 significant genetic diversity within the C. diphtheriae species, and ribotyping and MEE data general

190 ently, cell wall extracts of a particular C. diphtheriae strain (DSM43989) lacking mycolic acid ester

191 We report that an htaA deletion mutant of C. diphtheriae strain 1737 is unable to use the Hb-Hp compl

192 ae, FimA, is expressed in corynebacteria, C. diphtheriae strain NCTC13129 polymerized FimA to form sh

193 demic of 1993 to 1998 and 13 non-Georgian C. diphtheriae strains (10 Russian and 3 reference isolates

194 Sixty-six Corynebacterium diphtheriae strains (62 of the gravis biotype and 4 of t

195 Toxigenic Corynebacterium diphtheriae strains cause diphtheria in humans.

196 One hundred fifty-six Corynebacterium diphtheriae strains from throughout Russia, selected for

197 nd the emergence of epidemic Corynebacterium diphtheriae strains globally have highlighted the need f

198 reservoir for the emergence of toxigenic C. diphtheriae strains if they possessed functional diphthe

199 closely related to each other than to the C. diphtheriae strains isolated in other parts of the Unite

200 atory element (dtxR) from 72 Corynebacterium diphtheriae strains isolated in Russia and Ukraine befor

201 A total of 26 nontoxigenic C. diphtheriae strains isolated in the United Kingdom durin

202 strated that the majority (87.5%; 7/8) of C. diphtheriae strains represented new sequence types (STs)

203 We show that several C. diphtheriae strains use the hemoglobin-haptoglobin (Hb-H

204 PD typing, ribotyping, and PFGE typing of C. diphtheriae strains were improved to enable rapid and co

205 When 23 toxigenic Corynebacterium diphtheriae strains, 9 nontoxigenic C. diphtheriae strai

206 erium diphtheriae strains, 9 nontoxigenic C. diphtheriae strains, and 44 strains representing the div

207 n hemoglobin-iron utilization, whereas in C. diphtheriae strains, deletion of hmuO caused no or only

208 phism [AFLP]) for the characterization of C. diphtheriae strains.

209 eight (40.0%) were caused by Corynebacterium diphtheriae strains; six were biovar mitis, which were a

210 endent regulatory protein in Corynebacterium diphtheriae that controls gene expression by binding to

211 d characterized two novel genetic loci in C. diphtheriae that encode factors that bind hemin and Hb.

212 an extracellular protein of Corynebacterium diphtheriae that inhibits protein synthesis and kills su

213 iron-dependent repressor in Corynebacterium diphtheriae that regulates transcription from multiple p

214 eted by lysogenic strains of Corynebacterium diphtheriae, that causes the disease diphtheria in human

215 y human pathogens, including Corynebacterium diphtheriae, the causative agent of diphtheria, use host

216 Corynebacterium diphtheriae, the causative agent of diphtheria, utilizes

217 Corynebacterium diphtheriae, the causative agent of the severe respirato

218 Corynebacterium diphtheriae, the etiologic agent of diphtheria, utilizes

219 In Corynebacterium diphtheriae, the pilin-specific sortase SrtA catalyses p

220 livered to the cytoplasm of non-lysogenic C. diphtheriae, they integrated into either the attB1 or at

221 toxin produced by the causative organism, C. diphtheriae; this detection is the definitive test for t

222 chtC gene has no affect on the ability of C. diphtheriae to use hemin or Hb as iron sources; however,

223 nd binary toxin (CDTa-CDTb), Corynebacterium diphtheriae toxin (DT), and Pseudomonas aeruginosa exoto

224 of a heterotrimeric pilus in Corynebacterium diphtheriae, uncovering the molecular switch that termin

225 ia toxin repressor (DtxR) of Corynebacterium diphtheriae uses Fe(2+) as a corepressor.

226 Corynebacterium diphtheriae utilizes hemin and hemoglobin (Hb) as iron s

227 The human pathogen Corynebacterium diphtheriae utilizes hemin and hemoglobin as iron source

228 C. diphtheriae was also able to use human serum albumin (HS

229 Corynebacterium diphtheriae was examined for the ability to utilize vari

230 gulatory protein, DtxR, from Corynebacterium diphtheriae was identified from T. pallidum.

231 Toxigenic C. diphtheriae was isolated from five members of four house

232 Total cellular RNA isolated from C. diphtheriae was used to identify the transcriptional sta

233 d the systems for genetic manipulation of C. diphtheriae, we constructed plasmid vectors capable of i

234 A C. diphtheriae zur mutant was more sensitive to peroxide st