ライフサイエンスコーパス: directed evolution

1 sually carried out by rational design and/or directed evolution.

2 anced the thermal stability of Cel7A through directed evolution.

3 seek improved catalytic characteristics via directed evolution.

4 is a common task in protein engineering and directed evolution.

5 made by chimeragenesis, rational design, or directed evolution.

6 ationally designed hydrophobic pocket during directed evolution.

7 previously realized from rational design or directed evolution.

8 starting points for further optimization by directed evolution.

9 protein properties using techniques such as directed evolution.

10 new approach to optimizing gene networks via directed evolution.

11 tions are needed to stabilize proteins using directed evolution.

12 riants that may emerge in natural as well as directed evolution.

13 synthetase mutants with new specificities by directed evolution.

14 ility and recombinant expression yield using directed evolution.

15 tive bacterial species, Escherichia coli, by directed evolution.

16 with interesting structural properties using directed evolution.

17 dary metabolite glycosyltransferase (GT) via directed evolution.

18 he opportunity to improve ArM performance by directed evolution.

19 ch an expanded genetic code is available for directed evolution.

20 ing enzymes through screening, selection and directed evolution.

21 ne to attain desired scFv properties through directed evolution.

22 imeric NRPSs can be functionally restored by directed evolution.

23 xhibited by the fastest mutants obtained via directed evolution.

24 ubstrate was enhanced more than 1000-fold by directed evolution.

25 e and they have yet to be engineered through directed evolution.

26 g site of an enzyme that can be subjected to directed evolution.

27 ning focused protein libraries to be used in directed evolution.

28 or those at others) is a notable feature of directed evolution.

29 ecular-recognition motifs identified through directed evolution.

30 retroaldolase designs prior to comprehensive directed evolution.

31 d mutagenesis, computational refinement, and directed evolution, a modestly active, computationally d

32 Using directed evolution, a variant N-acetyl amino acid racema

33 shes a generic platform for rapid eukaryotic-directed evolution across an array of target cargo.

34 Optimization of this activity by directed evolution afforded an efficient enzyme variant

35 DNA approaches have enabled the extension of directed evolution algorithms to the genome-scale.

36 In contrast, directed evolution also yielded CBL(P113S), which cataly

37 we combine ancestral protein reconstruction, directed evolution and biophysical analysis to explore a

38 We herein report the directed evolution and characterization of an orthogonal

39 Here we use a combination of directed evolution and chemical and biochemical approach

40 A combined directed evolution and computational design approach is

41 fluorescent properties of iLOV, we employed directed evolution and determined five LOV crystallograp

42 ssayed, has emerged as a powerful format for directed evolution and functional metagenomics but is cu

43 these new technologies within the context of directed evolution and inverse metabolic engineering.

44 Directed evolution and protein engineering approaches us

45 Extensive directed evolution and protein engineering resulted in s

46 ting Evolutionary Adaptive Paths (REAP), for directed evolution and protein engineering that exploits

47 ering new structural folds experimentally by directed evolution and random recombination of secondary

48 hermal green protein (TGP) and eCGP123 using directed evolution and ratiometric sorting.

49 In addition, modern methods of directed evolution and synthetic biology, especially tho

50 powerful techniques of genetic engineering, directed evolution, and biomimetics.

51 tural information, bacterial selection-based directed evolution, and combinatorial design.

52 n fields as varied as genomics, diagnostics, directed evolution, and drug screening.

53 onomeric FP, for enhancing its brightness by directed evolution, and for thoroughly characterizing th

54 Following one amino acid at a time path of directed evolution, and using the strategy of structure-

55 Herein we describe directed evolution applied to the integrin alpha(L) inse

56 An iterative directed evolution approach employing random mutagenesis

57 A directed evolution approach has been used for the genera

58 We therefore applied a directed evolution approach involving the generation of

59 Taken together, the directed evolution approach reveals an unexpectedly flex

60 t targets RSV F protein, were generated by a directed evolution approach that allowed convenient mani

61 We describe a directed evolution approach that should find broad appli

62 We used a directed evolution approach to examine the molecular mec

63 ormous potential of a combined computational/directed evolution approach to protein engineering.

64 First, with a directed evolution approach, we isolated mutations in D1

65 re then further optimized using a FACS-based directed evolution approach.

66 In parallel, protein design and directed evolution approaches are beginning to be exploi

67 either partner, enabling the application of directed evolution approaches for high-throughput charac

68 Inspired by the great success of directed evolution approaches in tailoring enzyme proper

69 Herein, we have combined rational design and directed evolution approaches to test this hypothesis.

70 In contrast to other in vivo directed evolution approaches, CPR largely mitigates hos

71 ction of a protein, researchers have adopted directed evolution approaches, which use iterated cycles

72 Biocatalyst discovery and directed evolution are central to many pharmaceutical re

73 d mutants of this monooxygenase generated by directed evolution are excellent catalysts for the oxida

74 absolute catalytic effect and the effect of directed evolution are reproduced and analyzed (assuming

75 enzymes created by computational design and directed evolution are versatile biocatalysts whose prom

76 ombinatorial strategy of rational design and directed evolution as a powerful tool for the discovery

77 enantioselectivity proved to be possible by directed evolution based on saturation mutagenesis (up t

78 ch design-based strategies, we report here a directed evolution-based method for generating optimal b

79 With the development of directed evolution beginning in the 1990s and continuing

80 erase orthologues from the genus Thermus and directed evolution by CSR in the presence of inhibitors.

81 the experimental library-size limitations of directed evolution by focusing diversity toward the posi

82 It augments recombination-based directed evolution by incorporating a strategy for stati

83 generating novel molecular diversity during directed evolution by random substitution of one contigu

84 fluorescent protein mPlum was developed via directed evolution by selection for red emission, and we

85 h smaller, nonantibody therapeutics, we used directed evolution by yeast-surface display to engineer

86 Therefore a directed evolution campaign with two rounds of random mu

87 ld take approximately 3-5 d, whereas a whole directed evolution can be performed in 3-10 rounds, depe

88 As an alternative approach, directed evolution can provide a rapid and efficient mea

89 Directed evolution can thus be used to enhance P450 2B1

90 C) protein via a novel Closed Loop Aptameric Directed Evolution (CLADE) approach.

91 Closed loop aptameric directed evolution, (CLADE) is a technique enabling simu

92 enzymes created by computational design and directed evolution constitute a potentially valuable sou

93 with the more empirical methods of classical directed evolution (DE) for improving kcat (where natura

94 We model the process of directed evolution (DE) in silico using genetic algorith

95 We note that directed evolution differs in a number of ways from natu

96 e activity of TEM, have been coupled using a directed evolution domain insertion approach.

97 itate nucleotidyltransferase mechanistic and directed evolution/engineering studies.

98 ms in immunology we may be able to perform a directed evolution experiment where we follow the evolut

99 ortantly, the consensus binder isolated from directed evolution experiments (6.2.18) forms a higher o

100 In addition, directed evolution experiments identified a mutation, V6

101 Directed evolution experiments identified I-TevI variant

102 While directed evolution experiments show that new functions c

103 We also conducted directed evolution experiments to evolve 412d beyond the

104 Here, using directed evolution experiments with the GIY-YIG homing e

105 n used as the starting model for a series of directed evolution experiments, resulting in mutants of

106 te state machines-which can be inferred from directed evolution experiments.

107 combining computational protein design with directed evolution for creating new enzymes, and we anti

108 this variant was then further engineered via directed evolution for practical application in a manufa

109 method should find widespread application in directed evolution for the assembly of gene libraries an

110 PTE has previously proven amenable to directed evolution for the improvement of catalytic acti

111 Directed evolution formats take advantage of the dynamic

112 tal test of evolutionary reversibility using directed evolution from a phosphotriesterase to an aryle

113 losonic acid (L-KDO) aldolase was created by directed evolution from the Escherichia coli D-Neu5Ac (N

114 Directed evolution generated 70 GR variants with absorpt

115 We highlight recent cases in which directed evolution generated enzymatic activities and su

116 Additional directed evolution generated two enzymes having NADH-dep

117 is now supports our original hypothesis that directed-evolution had generated an evolutionary interme

118 Engineering and study of protein function by directed evolution has been limited by the technical req

119 In the laboratory, directed evolution has emerged as a powerful tool for th

120 Aided by clever selection schemes, directed evolution has generated new parts for genetic c

121 Directed evolution has proved to be an effective strateg

122 Directed evolution has revealed that single amino acid m

123 Over the past two decades, directed evolution has transformed the field of protein

124 IFRS, a product of directed evolution, has distinct binding modes for diffe

125 mputational design approaches, combined with directed evolution, has provided an opportunity to revis

126 Directed evolution identified two point mutations that i

127 emonstrate its power, we apply the system to directed evolution, identifying new mutants of the enzym

128 of in silico molecular docking and in vitro directed evolution in a well-characterized drug-sensitiv

129 We used directed evolution in an organotypic human airway model

130 We previously used directed evolution in human airway epithelia to create a

131 We have initiated a program of directed evolution in order to create mutant amide synth

132 ts to RNA aptamers using rational design and directed evolution in Saccharomyces cerevisiae.

133 of wild-type AAV genomes from tissues during directed evolution in the absence of wild-type adenoviru

134 By directed evolution in the laboratory, we previously gene

135 the gene encoding pocr to several rounds of directed evolution in which codons for the corresponding

136 Directed evolution, in addition to its principal applica

137 ncrease affinity are typically identified by directed evolution involving combinatorial libraries.

138 sence of detailed mechanistic understanding, directed evolution is a powerful complement to 'rational

139 It is well known that directed evolution is a powerful method for creating pro

140 Directed evolution is a powerful strategy for protein en

141 Directed evolution is a powerful tool for the creation o

142 Directed evolution is a powerful tool to improve the cha

143 Directed evolution is a powerful, but experimentally str

144 Directed evolution is a valuable technique to improve en

145 Directed evolution is a widely-used engineering strategy

146 Directed evolution is an important tool for overcoming t

147 Directed evolution is applied to increase the dynamic ra

148 Directed evolution is the experimental improvement of bi

149 and their improved variants from laboratory directed evolution (LDE), using atomistic simulations wi

150 Optimization by directed evolution led to the identification of two prom

151 In an analysis of a directed evolution longitudinal yeast data set, we are a

152 nered replication (CPR) is an emulsion-based directed evolution method based on a robust and modular

153 We have successfully developed a new directed evolution method for generating integral protei

154 This protocol describes a directed evolution method for in vitro mutagenesis and r

155 ELMA (SELection with Modified Aptamers) is a directed evolution method which can be used to develop D

156 Using a directed-evolution method that takes advantage of the na

157 While directed evolution methods for altering RO function have

158 Although in vitro and directed evolution methods have created genetic variants

159 While directed evolution methods have provided valuable tools

160 Most existing directed evolution methods, both in vivo and in vitro, s

161 When combined with directed evolution, molecular dynamics simulations, and

162 Unexpectedly, it is found that the directed evolution mutants lead to the reduction of solv

163 Directed evolution of 2-keto-3-deoxy-6-phosphogalactonat

164 Here we report the directed evolution of a ligand trap for Ang2 by harnessi

165 We use this system to perform the directed evolution of a phosphotriesterase (a bioremedia

166 We consider the directed evolution of a population after an intervention

167 Here we describe a strategy using in vitro-directed evolution of a single TCR to change its peptide

168 step one, we target a protein of interest by directed evolution of a small beta-sheet scaffold; in st

169 of its negatively charged amino acids using directed evolution of a synthetic form of Ocr, termed po

170 recombinant AAV-packaging cell lines and the directed evolution of AAV capsids.IMPORTANCE We first re

171 Directed evolution of an engineered beta-isopropylmalate

172 Previously we reported the directed evolution of an intein-based molecular switch i

173 Here we report the directed evolution of an iron-containing enzymatic catal

174 Directed evolution of Arabidopsis thaliana phytochelatin

175 This protocol describes the directed evolution of artificial endonuclease and ligase

176 Directed evolution of B. napus DGAT1 (BnaDGAT1) previous

177 s activated alpha(IotaIotab)beta(3), we used directed evolution of beta(3)(K716A) to identify substit

178 The directed evolution of biomolecules to improve or change

179 sign a new M13 phagemid-based system for the directed evolution of biomolecules.

180 ructuring mechanisms are key elements in the directed evolution of cellular metabolic pathways.

181 Thus, the knowledge generated from directed evolution of DGAT1 in one plant species can be

182 le tools for high-throughput screens for the directed evolution of efficient nerve agent organophosph

183 To investigate this phenomenon, we used directed evolution of EmrE to assess the rate of develop

184 many new applications: for example, assay of directed evolution of enzymes catalyzing halo-metabolite

185 protein engineering permit the design and/or directed evolution of enzymes specifically tailored for

186 powerful platform for single cell analysis, directed evolution of enzymes, and high throughput scree

187 , which exhibits features reminiscent of the directed evolution of enzymes, delivers catalysts that c

188 echnology enables the in vitro selection and directed evolution of functional proteins from libraries

189 and are not used for parallel and continuous directed evolution of gene networks or genomes.

190 escribe a system that enables the continuous directed evolution of gene-encoded molecules that can be

191 The reporter enabled in vivo directed evolution of Gerbera hybrida 2-pyrone synthase

192 nd corticosterone, were chosen to assist the directed evolution of hERalphaLBD.

193 a highly sensitive selection method for the directed evolution of homing endonucleases that couples

194 Directed evolution of maize HPPD was accomplished by pro

195 system should also be readily applicable for directed evolution of other DNA cleavage enzymes.

196 Two screening processes were developed for directed evolution of Pdc1 expressed in Escherichia coli

197 Here we describe a system for the continuous directed evolution of proteases using phage-assisted con

198 Alternative search strategies for the directed evolution of proteins are presented and compare

199 munized animals and/or performing laboratory-directed evolution of proteins with an existing low affi

200 in libraries, and should enable the in vitro directed evolution of proteins with designer single-mole

201 ggesting that PERMUTE will be useful for the directed evolution of proteins with new functions.

202 code can confer a selective advantage in the directed evolution of proteins with specific properties.

203 ion of unnatural amino acids in vivo and the directed evolution of proteins, show promise in elucidat

204 Using directed evolution of RlmN under antibiotic selection, w

205 Directed evolution of Rma cyt c in the bacterial catalys

206 Through directed evolution of SecB, we have identified and chara

207 Early studies on the directed evolution of serine recombinase DNA sequence sp

208 a selection mechanism for the isolation and directed evolution of ternary complexes where unnatural

209 Here, we show that structure-guided directed evolution of the active site of the cytochrome

210 nstrate our approach using as an example the directed evolution of the bacteriophage lambda cI TF aga

211 for the target function followed by stepwise directed evolution of the corresponding protein along th

212 Herein, we report the directed evolution of the fluorinase FlA1 for improved c

213 We conducted directed evolution of the Ir(Me)-myoglobin and generated

214 Via directed evolution of the natural iron-sequestering ferr

215 creen and the first proof of concept for the directed evolution of this enzyme class toward the ident

216 Overall, the directed evolution of three different designed Kemp elim

217 amatically decreases the screening effort in directed evolution of transaminases, as only active vari

218 Directed evolution of two computationally designed Kemp

219 gainst an entirely combinatorial approach of directed evolution of vast protein libraries.

220 king further affinity maturation in vitro by directed evolution or computational design necessary.

221 uld be useful in high throughput screens for directed evolution or drug discovery.

222 sensitive fluorescent reporter proteins and directed evolution or mutagenesis of proteins in general

223 y of recombinases can be altered either with directed evolution or via fusions to modular DNA-binding

224 y provide solutions to otherwise intractable directed evolution problems and address novel questions

225 ere included in the libraries throughout the directed evolution process.

226 These directed evolution processes dramatically enhanced both

227 Most in vitro directed evolution processes utilize nucleotide content

228 Directed evolution produced an unexpected combination of

229 in crude lysates and can be applied to other directed evolution projects.

230 bility to optimize non-natural reactivity by directed evolution promises to yield exceptional catalys

231 uce a high-fidelity, high-throughput Rubisco-directed evolution (RDE2) screen that negates false-posi

232 llowing this template of rational design and directed evolution, Rec(ZF)s may eventually mediate gene

233 screening of 17 cut sites in HRP followed by directed evolution, reconstitute into an active form whe

234 Directed evolution relies on both random and site-direct

235 Directed evolution relies on iterative cycles of randomi

236 Directed evolution remains a powerful, highly generaliza

237 Five generations of directed evolution resulted in yeast pyruvate decarboxyl

238 eliminated by using protein engineering and directed evolution, resulting in superior probes for use

239 We utilized a combination of directed evolution, saturation mutagenesis, chemical mod

240 We then applied directed evolution so as to alter the recognition of a p

241 Here, we describe a directed evolution strategy for enzymes that catalyze, i

242 s this problem, we have created a two-tiered directed evolution strategy in Escherichia coli that ena

243 We propose that this structure-directed evolution strategy might be generally applicabl

244 Directed evolution studies are enabled through the const

245 uct) is the basis for using microdroplets in directed evolution studies, and the approaches described

246 nction, in no insignificant part a result of directed evolution studies, are increasingly empowering

247 indicating that this method can be used for directed evolution studies.

248 and also provides support for mutagenesis or directed evolution studies.

249 Directed evolution techniques (random mutagenesis and hi

250 Here we applied directed evolution techniques to create magnetic resonan

251 ld include determining how computational and directed-evolution techniques can be implemented to incr

252 1) as a proof of principle, we show that two directed evolution technologies that we recently develop

253 ctivity and give insight into the process of directed evolution that gave rise to this family of neut

254 Using directed evolution, the properties of a beta-class CA fr

255 everal useful RFPs have been developed using directed evolution, the quest for further red-shifted an

256 Non-natural activities can be improved by directed evolution, thus mimicking the process used by n

257 Recent advances have focused on applying directed evolution to a variety of enzymes, such as epox

258 Here we apply part mining and directed evolution to build a set of transcriptional AND

259 e engineering of a cytochrome P450 enzyme by directed evolution to catalyze metal-oxo-mediated anti-M

260 We have used directed evolution to construct IL-2 mutants that bind t

261 e combine substrate specificity analysis and directed evolution to develop a diverse collection of Gi

262 E. coli.To overcome this problem, we applied directed evolution to E. coli ribosomal protein S1.

263 This constitutes the first reported use of directed evolution to enable the functionalization of su

264 Previously, we used directed evolution to engineer knottin peptides that bin

265 To address this need, we applied in vivo directed evolution to engineer potent retrograde functio

266 or production of Trp analogs and for further directed evolution to expand substrate and reaction scop

267 We used directed evolution to explore allosteric regulation as a

268 a zinc finger scaffold, followed by in vitro directed evolution to further optimize these enzymes.

269 high affinity antagonists to TSST-1, we used directed evolution to generate a panel of high affinity

270 We used directed evolution to identify mutations that dramatical

271 that are being developed to allow us to use directed evolution to improve enzyme properties, often d

272 ll molecules is dependent upon techniques in directed evolution to improve production titers.

273 To this end, we used in vitro-directed evolution to increase the affinity of human CD4

274 Cytochrome P450 2B1 has been subjected to directed evolution to investigate the role of amino acid

275 isplay of Mac-1 inserted (I) domain library, directed evolution to isolate active mutants of the I do

276 cally disordered region has been modified by directed evolution to quantitatively assess the thermody

277 Here, we combine rational design and directed evolution to re-engineer the serine recombinase

278 We have applied directed evolution to TrpB from Pyrococcus furiosus and

279 E(p4), a variant previously obtained through directed evolution, transforms several chlorobiphenyls,

280 turis pennsylvanica that was optimized using directed evolution (Ultra-Glo, Promega).

281 motifs from mutant libraries by experimental directed evolution under no co-evolutionary constraints.

282 In this study, directed evolution using a surrogate loop approach and y

283 rom the 14-kDa photoactive yellow protein by directed evolution using yeast display and fluorescence-

284 , Vbeta) was subjected to multiple rounds of directed evolution using yeast display.

285 gineering that combines rational design with directed evolution, using a yeast surface display high-t

286 Directed evolution, using active-site randomization and

287 Structure-based directed evolution utilizing iterative saturation mutage

288 op reagents suitable for diagnosis, in vitro directed evolution was applied to identify consensus pep

289 cle, the only previous example of continuous directed evolution was the landmark study of Wright and

290 Directed evolution was used to discover a further gain-o

291 Directed evolution was used to engineer a small, disulfi

292 Using mRNA display selection and directed evolution, we designed novel antibody-like prot

293 Using directed evolution, we engineered a high-affinity cameli

294 Using directed evolution, we enhanced the catalytic function o

295 Here we describe how, using directed evolution, we have created a novel switch that

296 Using directed evolution, we isolated mutants of the beta and

297 odule for protein engineering, two rounds of directed evolution were performed to improve the activit

298 mining the structures of enzymes produced by directed evolution, where the specificity determinants m

299 By combining directed evolution with deep sequencing, it is now possi

300 ts on throughput imposed during the standard directed evolution workflow (library construction, trans