ライフサイエンスコーパス: directed mutagenesis

1 atalytic residues were identified using site-directed mutagenesis.

2 ated the predicted interface in vivo by site-directed mutagenesis.

3 two glutamate residues were changed by site-directed mutagenesis.

4 substituted by a cysteine codon through site-directed mutagenesis.

5 tudies by x-ray crystallography, and by site-directed mutagenesis.

6 te in IL-23R by the use of deletion and site-directed mutagenesis.

7 critical residues for 26D1 binding via site-directed mutagenesis.

8 uch as targeted genome modification and site-directed mutagenesis.

9 genase I from Pyrococcus furiosus using site directed mutagenesis.

10 ntly validated these candidate sites by site-directed mutagenesis.

11 Four mutants were obtained via site-directed mutagenesis.

12 topology whose structure we support by site-directed mutagenesis.

13 amino acid residues were validated with site-directed mutagenesis.

14 and their roles are investigated using site-directed mutagenesis.

15 cardiac RyR (RyR2) via structure-guided site-directed mutagenesis.

16 asparagines in various combinations via site-directed mutagenesis.

17 PS interactions, which are validated by site-directed mutagenesis.

18 ants on both allergens was performed by site-directed mutagenesis.

19 ch to stabilize full-length antibody by site-directed mutagenesis.

20 on, and we investigated these by random site-directed mutagenesis.

21 of these binding sites was confirmed by site-directed mutagenesis.

22 DNA molecules with homologous ends for site-directed mutagenesis.

23 tween ubiquitin and PLP2 were probed by site-directed mutagenesis.

24 ated conformationally locked mutants by site-directed mutagenesis.

25 site in the ion pore that we confirm by site-directed mutagenesis.

26 ype 2a-derived HCV clone (Jc1Gluc2A) by site-directed mutagenesis.

27 ort signal, and could be inactivated by site-directed mutagenesis.

28 with native mass spectrometry (MS) and site-directed mutagenesis.

29 CAT2 promoter activity was analyzed by site directed mutagenesis.

30 , adding a dynamic dimension to protein site-directed mutagenesis.

31 rminal domains, molecular modeling, and site-directed mutagenesis, a molecular basis for the observed

32 enzyme in combination with deletion and site-directed mutagenesis allowed identification of its unusu

33 a genetically encoded peptide scaffold, site-directed mutagenesis allows for rapid generation of anal

34 data and a catalytic model supported by site-directed mutagenesis allows full comparison with dicot C

35 Biochemical and site-directed mutagenesis analyses revealed, in contrast to t

36 We have carried out an extensive site-directed mutagenesis analysis of the CD16A receptor comp

37 In this study, site-directed mutagenesis analysis showed that the R128A or R

38 We have combined site-directed mutagenesis and activity assays with a structur

39 relocated by pressure was validated by site-directed mutagenesis and by inhibition by small peptides

40 residues between E. coli RF1 and RF2 by site-directed mutagenesis and characterized their preference

41 , and blocked by reduced temperature or site-directed mutagenesis and chemical derivatization of the

42 Using site-directed mutagenesis and computational modeling, we stud

43 In this report, combining single site-directed mutagenesis and double mutant analyses, we cond

44 Site-directed mutagenesis and electrophysiological analysis i

45 lumen, which could both be confirmed by site-directed mutagenesis and electrophysiology.

46 Here, using site-directed mutagenesis and exploiting the simplicity of ta

47 acids, which can be altered readily by site-directed mutagenesis and expressed in vitro and in vivo,

48 36 receptor antagonist protein by using site-directed mutagenesis and expression in HEK293T cells.

49 Site-directed mutagenesis and functional analyses revealed th

50 Through site-directed mutagenesis and functional analysis, we found t

51 e x-ray scattering and a combination of site-directed mutagenesis and functional assays, we show that

52 Site-directed mutagenesis and gene rescue studies show that A

53 nd infected cells, and we identified by site-directed mutagenesis and green fluorescent protein (GFP)

54 leus of HTB-9 cells, as demonstrated by site-directed mutagenesis and green fluorescent protein (GFP)

55 Here, we use site-directed mutagenesis and heterologous expression of chan

56 Combinations of site-directed mutagenesis and homogenous time-resolved fluore

57 Site-directed mutagenesis and homology models show the import

58 These findings were corroborated by site-directed mutagenesis and identification of bestatin as a

59 Site-directed mutagenesis and kinetics demonstrated that Asp-

60 Using a combination of site-directed mutagenesis and locked nucleic acids (LNA) comp

61 Molecular dynamics simulations, point-directed mutagenesis and mass spectrometry demonstrate t

62 olled proteolysis, gel electrophoresis, site-directed mutagenesis and microsecond MD simulations.

63 y we explored this binding site through site-directed mutagenesis and molecular dynamics simulations.

64 -3 and a Thr residue at P-2 By means of site-directed mutagenesis and NMR spectroscopy, we have been

65 ole in VU591-dependent inhibition using site-directed mutagenesis and patch-clamp electrophysiology.

66 We used site-directed mutagenesis and patch-clamp recordings to probe

67 The structure is confirmed by site-directed mutagenesis and provides a molecular framework

68 e promoter activity assay combined with site-directed mutagenesis and sequential chromatin immunoprec

69 es) is demonstrated here by a combination of directed mutagenesis and spectroscopic techniques, among

70 of new inhibitors was characterized by site-directed mutagenesis and structure-activity relationship

71 By site-directed mutagenesis and structure-guided analyses, we d

72 ning procedures (insertions, deletions, site-directed mutagenesis and sub-cloning).

73 Structural analysis combined with site-directed mutagenesis and the scintillation proximity rad

74 A three-dimensional model-guided site-directed mutagenesis and the use of defined disaccharide

75 erstood, we used its structure to guide site-directed mutagenesis and to dissect its function.

76 utational observations are validated by site-directed mutagenesis and transcript cleavage assays, and

77 We used site-directed mutagenesis and transient-kinetic approaches to

78 regulating Pi release was supported by site-directed mutagenesis and transport assays.

79 Site-directed mutagenesis and truncation analysis of ATR1 sug

80 ochondrial function, as demonstrated by site-directed mutagenesis and use of STAT3 knockout and mitoc

81 We show through site-directed mutagenesis and X-ray crystallography that this

82 Site-directed mutagenesis and yeast-two hybrid assays identif

83 coupled to MS, computational modeling, site-directed mutagenesis, and analysis of the CYP46A1 crysta

84 gh a combination of SPOT peptide array, site-directed mutagenesis, and bio-layer interferometry, we i

85 cs studies can be directly tested using site-directed mutagenesis, and findings from transposon studi

86 ethods including chemical modification, site-directed mutagenesis, and fluorescent spectroscopy, we d

87 In silico molecular modeling, site-directed mutagenesis, and functional assays demonstrate

88 ing combinations of molecular modeling, site-directed mutagenesis, and homogenous time-resolved FRET,

89 ping was performed by pepscan analysis, site-directed mutagenesis, and hydrogen/deuterium exchange-ma

90 kB2, activity assays, solution studies, site-directed mutagenesis, and in vivo complementation were u

91 crystal structures, molecular docking, site-directed mutagenesis, and kinetic and thermodynamic anal

92 , were deduced from crystal structures, site-directed mutagenesis, and kinetic and thermodynamic anal

93 tensive molecular dynamics simulations, site-directed mutagenesis, and kinetic measurements that char

94 of Tg for SPCA1a by in silico modeling, site-directed mutagenesis, and measuring the potency of a ser

95 cations, mass spectrometric techniques, site-directed mutagenesis, and molecular modeling, we show th

96 onal antibody (3C3G3) by phage display, site-directed mutagenesis, and surface plasmon resonance.

97 tion of NMR, fluorescence spectroscopy, site-directed mutagenesis, and thermodynamics to elucidate th

98 relationships, using electrophysiology, site-directed mutagenesis, and voltage-clamp fluorometry.

99 Using MS analysis, site-directed mutagenesis, and X-ray structural data analysis

100 used a stepwise, sequential, cumulative site-directed mutagenesis approach, based on rebuilding the A

101 emical labeling, mass spectrometry, and site-directed mutagenesis approaches, we show that thiram (an

102 Crystal structures and site-directed mutagenesis are routinely used to identify thes

103 a unique cysteine residue introduced by site-directed mutagenesis) as free-radical trapping 'tags' fo

104 pinpointed by computational results and site-directed mutagenesis at Ala33.

105 A combination of site-directed mutagenesis, biochemical and electrophysiologic

106 By combining site-directed mutagenesis, biochemical assays, and spectrosco

107 Using site-directed mutagenesis, cDNAs were generated in which Tyr(

108 s, NMR titrations, DNA-binding studies, site-directed mutagenesis, charge distribution, and sequence

109 ous complementary approaches, including site-directed mutagenesis, chemical cross-linking, peptide ma

110 Using luciferase reporter assays, site-directed mutagenesis, ChIP assays, and mithramycin A tre

111 KEY MESSAGE: Successful site-directed mutagenesis combined with in silico modeling an

112 Site-directed mutagenesis combined with kinase assays and spe

113 Direct binding assays, combined with site-directed mutagenesis, confirm that the primary collagen-

114 Site-directed mutagenesis confirmed residues involved in bind

115 Truncation mutations and site-directed mutagenesis confirmed that the KH domain in DDX

116 The molecular dynamics simulation and site-directed mutagenesis confirmed the important roles of th

117 Site-directed mutagenesis confirms the observed interaction i

118 Using a combination of site-directed mutagenesis, DA-uptake, and cross-linking exper

119 Site-directed mutagenesis data confirm the importance of Asp-

120 ive structure-activity relationship and site-directed mutagenesis data facilitates the prediction of

121 n- and loss-of-function experiments and site-directed mutagenesis demonstrated that TonEBP binding to

122 Site-directed mutagenesis disclosed residues Phe(241) and Ser

123 Site-directed mutagenesis established a requirement for ERK-m

124 Site-directed mutagenesis established that the N-terminal dom

125 Sequence analyses and site-directed mutagenesis established the importance of histi

126 Site-directed mutagenesis establishes that a hydrophobic clus

127 lyzable thioether linkage as well as by site-directed mutagenesis, evaluation of the pH dependence of

128 teine (Cys) residues were eliminated by site-directed mutagenesis, except an introduced E294C mutatio

129 large-scale molecular simulations and a site-directed mutagenesis experiment of a key residue.

130 cysteines within the mature peptide and site-directed mutagenesis experiments demonstrated that the f

131 Site-directed mutagenesis experiments have implicated a basic

132 Here, we use site-directed mutagenesis experiments in transgenic tobacco g

133 profiling on pulse overexpression, and site-directed mutagenesis experiments using a heterologous re

134 ing molecular evolutionary analyses and site-directed mutagenesis experiments, we provide evidence fo

135 with previous cross-linking results and site-directed mutagenesis experiments.

136 ge coupling effects and predictions for site-directed mutagenesis experiments.

137 strate interactions were examined using site-directed mutagenesis followed by steady state kinetic an

138 Here, we report a form of oligonucleotide-directed mutagenesis for precision genome editing in pla

139 ts of the classic T7-lac promoter using site-directed mutagenesis, generating a panel of inducible hy

140 tween mammalian and archaeal ADPGK, and site-directed mutagenesis has confirmed residues essential fo

141 By combining site-directed mutagenesis, heterologous expression, and molec

142 -driven hENT3 nucleoside transport with site-directed mutagenesis, homology modeling, and [(3)H]adeno

143 nd computational analysis together with site-directed mutagenesis identified five basic residues dist

144 Systematic site-directed mutagenesis identified the core amino acid resi

145 Site-directed mutagenesis identified the precise site at whic

146 Promoter deletion and site-directed mutagenesis identified three functional sterol

147 Additionally, site-directed mutagenesis identifies essential residues and m

148 Molecular modeling and site-directed mutagenesis implicate several residues around t

149 , we addressed these questions by using site-directed mutagenesis in combination with enzymatic, tran

150 through VWF patient samples and through site-directed mutagenesis in the VWF A1 domain can decrease o

151 ion of alpha-terpineol and 1,8-cineole, site-directed mutagenesis, in silico modeling, and semiempiri

152 ilization of the coiled-coil domains by site-directed mutagenesis increases the effective diffusivity

153 Furthermore, structure-guided site-directed mutagenesis indicated that residues in Pf-Avr4

154 Computational modeling and site-directed mutagenesis indicated that the mode of fatty ac

155 Site-directed mutagenesis indicates that tyrosine 645 in this

156 Site directed mutagenesis is widely used to understand the st

157 ul when cloning multiple fragments, for site-directed mutagenesis it is unnecessary.

158 Using site-directed mutagenesis, kinetic assays, and quantitative m

159 By site-directed mutagenesis, L249P was identified as the critic

160 We also demonstrated that site-directed mutagenesis leading to substitution of cleavage

161 Molecular modeling and site-directed mutagenesis led to creation of a mutant FgaPT2_

162 Site-directed mutagenesis, ligand-binding measurements, stead

163 Site-directed mutagenesis, mass spectrometry, and kinetic ana

164 The QuikChange site-directed mutagenesis method is popular but imperfect.

165 Compared with alternative site-directed mutagenesis methods, our protocol requires less

166 Here, we combine electrochemistry, site-directed mutagenesis, molecular dynamics and quantum che

167 To further assess pathogenicity, site-directed mutagenesis, mouse and human brain expression a

168 the model, including bacterial growth, site-directed mutagenesis, mouse inoculation (from cultured c

169 Using site-directed mutagenesis, NMR spectroscopy, and computer mod

170 Furthermore, site-directed mutagenesis of a predicted ETS-binding site wit

171 Site-directed mutagenesis of a putative ubiquitination site o

172 We describe in this paper a systematic site-directed mutagenesis of an endoglycosidase from Streptoc

173 Site-directed mutagenesis of any of the two conserved catalyt

174 Molecular modeling and site-directed mutagenesis of ARH3 revealed that numerous resi

175 molecular-dynamics (MD) simulations and site-directed mutagenesis of Cpx and SNAREs in Drosophila.

176 Site-directed mutagenesis of cysteine 175 or residues on the

177 mportance of its C-terminal region, and site-directed mutagenesis of each nonconserved residue in thi

178 ly validated through binding assays and site-directed mutagenesis of functional interfaces.

179 of the iron-bound CO and NO ligands by site-directed mutagenesis of Glu-87 and His-89.

180 Site-directed mutagenesis of GluN1-Glu781 reduced the potency

181 spectroscopy, heme quantification, and site-directed mutagenesis of histidine residues, we demonstra

182 Here we use molecular modeling and site-directed mutagenesis of hNaCT followed by transport char

183 Site-directed mutagenesis of IRE1alpha(Cys931) prevented S-ni

184 Site-directed mutagenesis of Lys-85 and Thr-86 in helix 1 rev

185 Site directed mutagenesis of lysine 68 to glutamine (K68Q), m

186 structure of the AT1R was used to guide site-directed mutagenesis of outward-facing hydrophobic resid

187 nserved D-H-S residues was probed using site-directed mutagenesis of PkPSD.

188 e models such as interpreting/designing site-directed mutagenesis of proteins.

189 Site-directed mutagenesis of putative cleavage sites identifi

190 Site-directed mutagenesis of PxaTPS8 revealed several catalyt

191 Site-directed mutagenesis of RadH was used to identify cataly

192 Site-directed mutagenesis of RCAR1 showed that its tyrosine r

193 Furthermore, by site-directed mutagenesis of residues at the trimerization in

194 Site-directed mutagenesis of residues involved in the respect

195 Site-directed mutagenesis of selected amino acids within ALMT

196 Site-directed mutagenesis of sequentially highly similar Msex

197 he engineered Cys239 mAb indicates that site directed mutagenesis of Ser239 to cysteine has no impact

198 Site-directed mutagenesis of some p37 sequence traits, includ

199 By site-directed mutagenesis of SR34 RNA-binding sequences and A

200 encoded by CHO1 Truncation analysis and site-directed mutagenesis of the CHO1 promoter indicated that

201 -terminal receptor peptides followed by site-directed mutagenesis of the cleavage sites links recepto

202 We performed site-directed mutagenesis of the L protein of vesicular stoma

203 Further, site directed mutagenesis of the miR-134 binding site in Sabi

204 Here, using site-directed mutagenesis of the mitochondrial COX1 gene from

205 Site-directed mutagenesis of the phytaspase cleavage sites in

206 In this study, we made site-directed mutagenesis on the surface-exposed hydrophobic

207 vivo and in vitro approaches including site-directed mutagenesis, phage plaque assays, circular dich

208 mass spectrometry, truncation analysis, site-directed mutagenesis, phosphopeptide mapping, and phosph

209 Using directed mutagenesis, phosphorylation of alphaPIX Rho-GE

210 Site-directed mutagenesis, protein biochemical, and structura

211 tures of four CBMs, in conjunction with site-directed mutagenesis, provide insight into the mechanism

212 ipitation (ChIP), promoter cloning, and site-directed mutagenesis, real-time quantitative PCR (RT-qPC

213 its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydrogen-deu

214 Remarkably, cccA deletion or site-directed mutagenesis resulted in an almost complete loss

215 Crystal structures of RPE65 and site-directed mutagenesis reveal aspects of its catalytic mec

216 Site directed mutagenesis revealed an essential role in NO re

217 ChIP and site-directed mutagenesis revealed prominent hypoxia response

218 s by top-down MS, NMR spectroscopy, and site-directed mutagenesis revealed specific and well-conserve

219 Site-directed mutagenesis revealed that GRK2 Ser-685 phosphor

220 Site-directed mutagenesis revealed that the McpM precursor pe

221 enetic analysis, sequence alignment and site-directed mutagenesis revealed that the region immediatel

222 ected patients (n = 168), together with site-directed mutagenesis, revealed Nef position 9 as a subty

223 Site-directed mutagenesis reveals that C191 and C192 are palm

224 Site-directed mutagenesis reveals that this interaction promo

225 polymorphisms (SNPs) as a surrogate of site-directed mutagenesis reveals the sequence dependency of

226 Site-directed mutagenesis (SDM) of these two sites demonstrat

227 orylated by AMPK at three sites, and by site-directed mutagenesis, Ser304 phosphorylation is importan

228 Mass spectrometry and site-directed mutagenesis showed that chemically distinct HNO

229 Site-directed mutagenesis showed that Glu-713, Leu-716, and L

230 Published modeling and site-directed mutagenesis studies had previously shown that t

231 lis-Menten, competitive inhibition, and site-directed mutagenesis studies identified exosite 2 as the

232 Site-directed mutagenesis studies indicate that amino acid re

233 Site-directed mutagenesis studies of K65R and T69del assessed

234 Site-directed mutagenesis studies on this enzyme and its subs

235 Site-directed mutagenesis studies revealed four substitutions

236 Site-directed mutagenesis studies showed that Cys-805, Cys-93

237 Site-directed mutagenesis studies suggest that at least predi

238 Extensive site-directed mutagenesis studies supported the importance of

239 studies of the proteins and a number of site-directed mutagenesis studies.

240 s of 1 was first conducted, followed by site-directed mutagenesis studies.

241 A site-directed mutagenesis study allowed the identification of

242 Site-directed mutagenesis substitution of the amino acids for

243 Mechanistic studies using site-directed mutagenesis suggest that, following initial enz

244 Molecular modeling-predicted and site-directed mutagenesis supported that this unique property

245 Through site-directed mutagenesis targeting the acidic pocket, we mod

246 cDNAs were constructed by site-directed mutagenesis that encode PORB mutant proteins wi

247 Here we show by site-directed mutagenesis that multiple residues within the S

248 Site-directed mutagenesis that swapped residue 153 between UG

249 Furthermore, we show by site-directed mutagenesis that tyrosine (Y382-384) within the

250 is-466, were also examined and shown by site-directed mutagenesis to be critical for CTQ biogenesis.

251 Site-directed mutagenesis to block HspB1 phosphorylation inhi

252 SagS domain was used as constructs and site-directed mutagenesis to elucidate how SagS performs its

253 In this study we used site directed mutagenesis to estimate the contribution of HLA

254 Here, we have made use of site-directed mutagenesis to examine the contribution of subu

255 onstructs, bioinformatics analysis, and site-directed mutagenesis to identify Lon domains and residue

256 We use mass spectrometry and site-directed mutagenesis to identify major sites of ADA3 ace

257 Site-directed mutagenesis to introduce single K458R, D445E, D

258 omplex, we used truncation variants and site-directed mutagenesis to investigate domains and residues

259 ative PKA phosphorylation sites and use site-directed mutagenesis to show that only phosphorylation a

260 In this report we used site-directed mutagenesis to substitute specific amino acid r

261 R) spectroscopic methods, combined with site-directed mutagenesis, to determine the mechanism of a si

262 ed x-ray crystallography, together with site-directed mutagenesis, to determine the minimal enzymatic

263 fect on STAT4 function was evaluated by site-directed mutagenesis using a lymphoblastoid B cell line

264 Directed mutagenesis was also used to further dissect th

265 s demonstrate that a rational design of site-directed mutagenesis was effective in producing a mutant

266 Site-directed mutagenesis was employed to map the sites of S-

267 Site-directed mutagenesis was performed by partial gene synth

268 In this study, site-directed mutagenesis was used to explore the hydrophobic

269 Site-directed mutagenesis was used to investigate the relativ

270 Site-directed mutagenesis was used to replace these side chai

271 Site-directed mutagenesis was used to show that the NADH:quin

272 ing computational molecular docking and site-directed mutagenesis we identify key residues within the

273 Using site-directed mutagenesis we restored all p-lsrR-box sites to

274 By site-directed mutagenesis we show that a histidine residue (H

275 Using truncation mutants and site-directed mutagenesis, we define the inhibitory face of t

276 Using site-directed mutagenesis, we demonstrate that key residues i

277 Using in vitro assays and site-directed mutagenesis, we demonstrate that the Nbp35 homo

278 Using luciferase assays and site directed mutagenesis, we demonstrate that these two elem

279 ce alignment, pH-activity profiles, and site-directed mutagenesis, we evaluated a series of active si

280 esonance energy transfer technique, and site-directed mutagenesis, we examined the domains involved i

281 Using site-directed mutagenesis, we found that ABCA1's PIP2 and pho

282 Using site-directed mutagenesis, we found that disruption of the st

283 crum." While validating the model using site-directed mutagenesis, we found that the Tyr-542 residue

284 Through systematic site-directed mutagenesis, we have discovered 12 taf2 tempera

285 nd mouse 5-HT3A subunits, and by use of site-directed mutagenesis, we have identified transmembrane a

286 combination of in silico prediction and site-directed mutagenesis, we have mapped an exosite to a non

287 Applying mutual information theory and site-directed mutagenesis, we identified an allosteric intrad

288 Using docking simulations and site-directed mutagenesis, we identified specific interaction

289 otease cleavage-predicting software and site-directed mutagenesis, we identified that calpain-1 cleav

290 g a random mutational screen as well as site-directed mutagenesis, we identify point mutations within

291 tic resonance titration experiments and site-directed mutagenesis, we located a potential contact reg

292 Using site-directed mutagenesis, we studied the effect on Physcomit

293 Using site-directed mutagenesis, we validated those surfaces in vit

294 Furthermore, by site-directed mutagenesis, we were able to identify amino aci

295 equences from one patient combined with site-directed mutagenesis, we were able to restore BST-2 anta

296 inally, in vitro PLK1 kinase assays and site-directed mutagenesis were employed to demonstrate that H

297 quential mutagenesis using swapping and site-directed mutagenesis, which identified residues critical

298 ellular localization were identified by site-directed mutagenesis, which identified serine residues 4

299 y lineage analysis and structure-guided site-directed mutagenesis with large-scale functional signali

300 nitrite over hydrogen peroxide by using site-directed mutagenesis, X-ray crystallography, (11)B NMR,